Exosomal rna transfer ala. Medical search. Frequent questions

to characterize exosomal RNA using the Agilent Bioanalyzer technology showed little to no trace of 18S and 28S rRNA in exosomes secreted by MC/9 murine mast cells, and similar conclusions were made by Skog et al. (wikipedia.org)
Moreover, exosomal miRNA-130a was transferred from CAFs to recipient NSCLC cells and knockdown of miRNA-130a reversed the effect of CAFs-derived exosomes during chemoresistance of NSCLC cells. (dovepress.com)
Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)
Taken together, these results suggest that CAFs-derived exosomes confer cisplatin resistance of NSCLC cells through transferring miRNA-130a and that PUM2 is a critical factor for packaging miRNA-130a into exosomes. (dovepress.com)
Chahar HS, Bao X, Casola A (2015) Exosomes and their role in the life cycle and pathogenesis of RNA viruses. (virosin.org)
Here, we report that plasma exosomes of COVID-19 patients contain SARS-CoV-2 double stranded RNA (dsRNA) and stimulate robust production of interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α), and other inflammatory cytokines and chemokines by human peripheral mononuclear cells. (wphobby.com)
Chen J, Wang S, Jia S, Ding G, Jiang G, Cao L. Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profile in Pancreatic Cancer Derived Exosomes Treated Dendritic Cells by Microarray Analysis. (jcancer.org)
For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)
We aimed to figure out whether exosomes and exosomal miRNA from necrotic bone tissues of patients with NONFH are involved in the pathogenesis of NONFH and reveal the underlying mechanisms. (biomedcentral.com)
We performed differential centrifugation to isolate exosomes from serum or conditioned media of cells and analyzed the impact of exosomal ERp44 on cells cisplatin sensitivity. (biomedcentral.com)
More importantly, our results showed under ERS, NPC cells secreted exosomes containing ERp44 and could transfer them to adjacent cells to strengthen chemoresistance. (biomedcentral.com)
Non-coding RNAs (ncRNAs) are enriched in exosomes, which participated in generation, progression, and resistance of BC. (biomedcentral.com)
The overlap between HIV-1 and exosome biogenesis in a infected cell shows that HIV-1 items, including RNA and proteins could be encased within exosomes or contaminate exosome arrangements from HIV-1 contaminated liquids [13C15]. (researchdataservice.com)
Within this research, we confirmed that exosomes can boost HIV-1 entrance into individual T and monocytic cell lines via exosomal tetraspanin protein Compact disc81 and Compact disc9. (researchdataservice.com)
Isolation CD81 of individual plasma-derived exosomes Plasma was gathered from whole bloodstream of individual donors into pipes containing ethylenediaminetetraacetic acidity (EDTA) and prepared as explained by Konadu et al [21] with some adjustments. (researchdataservice.com)

exRNA can be shielded from RNases by RNA binding proteins (RBPs), on their own or within/associated with lipoprotein particles and extracellular vesicles. (wikipedia.org)
EVs released from HLSCs were enriched with miRNAs and expressed Alix and several RNA-binding proteins, including Argonaute 2 (Ago2), a member of the Argonaute family known to be involved in the transport and the processing of miRNAs. (spandidos-publications.com)
EVs are a natural carrier system that can transfer nucleic acids, proteins, and lipids between donor and recipient cells in an autocrine, paracrine, and endocrine manner. (imrpress.com)
Exosome contain proteins, miRNAs, and mRNAs, and the exosomal lipid bilayer protects this genetic information from degradation. (biomedcentral.com)

In recent years, noncoding RNAs (ncRNAs), especially microRNAs, long noncoding RNAs and circular RNAs, have been found to be associated with abnormalities of the ASMCs. (ersjournals.com)
This review highlights findings concerning noncoding RNAs (ncRNAs) in asthmatic ASMCs so far and proposes a schematic diagram that helps us to better understand how ncRNAs are involved in pathophysiological behavioural changes in asthmatic ASMCs. (ersjournals.com)
Many noncoding RNAs (ncRNAs) have been identified in asthmatic ASMCs in recent years [ 5 ]. (ersjournals.com)
MicroRNAs (miRNAs) are the most altered subset of ncRNAs, followed by long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). (ersjournals.com)
Recently, noncoding RNAs (ncRNAs) have been reported to regulate protein expression, and it is definite that PTEN expression is controlled by ncRNAs epigenetically or posttranscriptionally as well. (biomedcentral.com)
However, the mechanism underlying the chemoresistance and metastasis in BC cells mediated by the BC-derived exosomal ncRNAs remained to be elucidated. (biomedcentral.com)

During vesiculation, nucleic acids, including mRNAs, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs or miRs) are encapsulated within EVs and when released, they are protected from degrading enzymes ( 8 - 10 ). (spandidos-publications.com)
MicroRNAs (miRNAs) are short non-coding RNAs that posttranscriptionally regulate gene expression inside the cell. (frontiersin.org)
Ever since the discovery of small non-coding RNAs, microRNAs have been identified to play a critical role in development and function of pancreatic insulin-producing beta cells. (edu.pl)

Extracellular RNA (exRNA) describes RNA species present outside of the cells in which they were transcribed. (wikipedia.org)
Carried within extracellular vesicles, lipoproteins, and protein complexes, exRNAs are protected from ubiquitous RNA-degrading enzymes. (wikipedia.org)
The United States National Institutes of Health (NIH) published in 2012 a set of Requests for Applications (RFAs) for investigating extracellular RNA biology. (wikipedia.org)
Funded by the NIH Common Fund, the resulting program was collectively known as the Extracellular RNA Communication Consortium (ERCC). (wikipedia.org)
Although RNAs can be excreted from the cell without an enveloping container, ribonucleases present in extracellular environments would eventually degrade the molecule. (wikipedia.org)
Extracellular RNA should not be viewed as a category describing a set of RNAs with a specific biological function or belonging to a particular RNA family. (wikipedia.org)
Similar to the term "non-coding RNA", "extracellular RNA" defines a group of several types of RNAs whose functions are diverse, yet they share a common attribute which, in the case of exRNAs, is existence in an extracellular environment. (wikipedia.org)
To function or even survive as full-length RNA in extracellular environments, exRNA must be protected from digestion by RNases. (wikipedia.org)
Extracellular vesicles in particular are thought to be a way to transport RNA between cells, in a process that may be general or highly specific, for example, due to incorporation of markers of the parent cell that may be recognized by receptors on the recipient cell. (wikipedia.org)
Extracellular miRNAs are capable of targeting mRNAs in the recipient cell through RNA interference pathways. (wikipedia.org)
The horizontal transfer of extracellular RNAs carried by EVs has been shown to be able to reprogram hematopoietic progenitors ( 11 ) and to activate endothelial cells ( 12 ). (spandidos-publications.com)
Furthermore, the inhibitors reduced the levels of extracellular and intracellular viral RNA. (virosin.org)
Intercellular mitochondrial transfer occurs in different ways, including tunnelling nanotubes (TNTs), extracellular vesicles (EVs), and gap junction channels (GJCs). (biomedcentral.com)
Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
CRC cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner, and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or paracrine. (besjournal.com)
Intercellular communication is mediated by extracellular vesicles (EVs), as they enclose selectively packaged biomolecules that can be horizontally transferred from donor to recipient cells. (biomedcentral.com)

The expression profiles of long noncoding RNAs (lncRNAs) and mRNAs of PEX-treated dendritic cells are unknown. (jcancer.org)

The following types of RNA have been found outside the cell: Messenger RNA (mRNA) Transfer RNA (tRNA) MicroRNA (miRNA) Small interfering RNA (siRNA) Long non-coding RNA (lncRNA) Though prevalent inside of the cell, ribosomal RNA (rRNA) does not seem to be a common exRNA. (wikipedia.org)
Messenger RNA contained in microvesicles secreted from glioblastomal cells were shown to generate a functional protein in recipient (human brain microvascular endothelial) cells in vitro. (wikipedia.org)
Messenger RNA was checked for quality (RIN value 5.0 for the samples used in the validation phase). (tam-receptor.com)

Circular RNAs (circRNAs) are noncoding RNAs with widespread distribution and diverse cellular functions. (biomedcentral.com)
CircRNAs have essential functions as microRNA sponges, RNA-binding protein- and transcriptional regulators, and protein translation templates. (biomedcentral.com)
Because of this extraordinary construction, circRNAs are resistant to degradation by exonucleases and are more stable than linear RNA. (biomedcentral.com)
Dysregulation of circular RNAs (circRNAs) has recently been found to play an important role in the progression and development of cancers such as non-small cell lung cancer (NSCLC). (biomedcentral.com)
Circular RNAs (circRNAs) belong to a class of noncoding and protein-coding RNAs localized to the cytoplasm which originate from both single and multiple exons [ 7 , 8 ]. (biomedcentral.com)

On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis. (pmoptics.com)

The Endosomal Sorting Complex Required for Transport (ESCRT) machinery was previously considered as a possible mechanism for RNA secretion from the cell, but more recently research studying microRNA secretion in human embryonic kidney cells and Cercopithecus aethiops kidney cells identified neutral sphingomyelinase 2 (nSMase2), an enzyme involved in ceramide biosynthesis, as a regulator of microRNA secretion levels. (wikipedia.org)
Treatment with GW4869 and si-Rab27a, two exosomal secretion inhibitors, inhibited exosome release. (virosin.org)
Such exosomal secretion of protein is likely to influence faraway cell signaling or promote a distinct segment that sustains tumor microenvironment resulting in disease spread. (biomasswars.com)

EVs were instrumental in transferring selected miRNAs from HLSCs to human endothelial cells absent in the latter cells. (spandidos-publications.com)
These results may represent a novel function of Alix, demonstrating its involvement in the EV-mediated transfer of miRNAs. (spandidos-publications.com)
The EV-mediated transfer of miRNAs is a particularly good candidate for the epigenetic alterations observed in recipient cells. (spandidos-publications.com)
In fact, it has been shown that EVs are enriched in miRNAs and that they can be transferred to target cells ( 13 , 14 ). (spandidos-publications.com)
Gibbings et al ( 16 ) suggested that the constituents of the miRNA effector complex, such as GW182 and Argonaute 2 (Ago2), are involved in the packaging of miRNAs within multivesicular bodies during exosome biogenesis. (spandidos-publications.com)
RNA polymerase II mainly transcribes microRNA genes as primary miRNA transcripts (pri-miRNAs) that contain 5′cap and 3′poly(A) tails ( 28 ). (frontiersin.org)
Drosha, RNase III, and DGCR8, the RNA-binding protein, further process pri-miRNAs into stem-loop structured precursor miRNAs (pre-miRNAs) of ~70 nt ( 29 ). (frontiersin.org)
After pre-miRNAs are transported to the cytoplasm, RNase III Dicer and TRBP (transactivation-response RNA-binding protein) cleave them into double-stranded miRNA duplexes of ~22 nt ( 30 , 31 ). (frontiersin.org)
Changes in exosomal miRNAs have been reported in patients diagnosed with Alzheimer's disease (AD), and miRNAs have been shown to provide diagnostic biomarkers [ 26 ]. (biomedcentral.com)

In vitro experiments have shown the transfer of specific exRNAs into recipient cells inhibiting protein expression and preventing cancer cell growth. (wikipedia.org)
For example RNA that is shuttled from one cell to another known as "exosomal shuttle RNA " could potentially impact protein production in the recipient cell [69]. (biomasswars.com)
The effects of PTX-induced exosomal circBACH1 on BC cell function were assessed using RNA Binding Protein Immunoprecipitation (RIP), dual luciferase reporter gene, tube formation, CCK-8, and Western Blot assays. (biomedcentral.com)
Together, our findings should motivate caution when interpreting conditional alleles, and suggest the exciting possibility of inter-tissue RNA or protein trafficking in modulation of reproductive biology. (elifesciences.org)

Then RT-qPCR was applied to test miR-21-5p level in tumor derived exosomal (TDE-miR-21-5p). (hindawi.com)
The subcutaneous tumor/caudal vein transfer mouse model was used for tumor growth and invasion analysis. (biomedcentral.com)

MiRNA usually binds directly to the 3′-untranslated region (3′-UTR) of target gene mRNA through the RNA-induced silencing complex (RISC) [ 15 , 16 ]. (ersjournals.com)

Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)

Alix knockdown did not influence the number of EVs released by HLSCs, but it significantly decreased miRNA expression levels in the EVs and consequently their transfer to the endothelium. (spandidos-publications.com)
Exosomal miRNA expression profiles may provide supplemental biomarkers for diagnosing and subtyping HFMD infections. (biomedcentral.com)

Microrna genes are transcribed by rna polymerase ii. (edu.pl)

Physique 3 elicits the RNA sequencing expression levels of the 10 genes selected CD28 for validation by qRT-PCR: Cytochrome P450 family 4 subfamily F member 22 (and displayed late amplification curves in several samples and were excluded from further analyses. (tam-receptor.com)
These results demonstrated that PTX-induced exosomal circBACH1 promoted stemness and migration of BC cells by sponging miR-217 to upregulate the expression of G3BP2, which provided a new therapeutic target for PTX-resistance and progression of BC via circBACH1/miR-217/G3BP2 axis. (biomedcentral.com)

Since 2010 he has been a PhD researcher at the Center for Neurosciences and Cell Biology (CNC-CIBB) at the University of Coimbra (UC), where he develops gene transfer systems for the development of disease models and treatment of neurodegenerative diseases. (cienciavitae.pt)
Currently he is an Assistant researcher at the Interdisciplinary Research Institute at University of Coimbra (III/UC), Researcher at the Vectors, Gene and Cell Therapy Group (CNC-CIBB) and Coordinator of the "ViraVector", a national strategic research infrastructure with the aim of developing viral vectors and carrying innovative gene transfer studies. (cienciavitae.pt)
Elegans heterochronic gene lin-4 encodes small rnas with antisense complementarity to lin-14. (edu.pl)

Fu Y, Zhang L, Zhang F, Tang T, Zhou Q, Feng C, Jin Y, Wu Z (2017) Exosome-mediated miR-146a transfer suppresses type I interferon response and facilitates EV71 infection. (virosin.org)

This study indicates that CAFs-derived exosomal miRNA-130a may be a potential therapeutic target for cisplatin resistance in NSCLC. (dovepress.com)

Aliotta et al ( 15 ) demonstrated that lung-derived vesicles carried RNA to marrow cells and altered the phenotype of these cells both genetically and functionally. (spandidos-publications.com)
Vesicles derived from adult human liver stem-like cells (HLSCs) have also been shown to contain ribonucleoproteins and different RNA species involved in hepatic regeneration ( 20 ). (spandidos-publications.com)
These vesicles can transfer signals capable of altering cell function and/or reprogramming targeted cells. (unicyte.ch)
This concept is based on the observation that vesicles may transfer different types of signals between cells [7, 8]. (unicyte.ch)

In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)
Intercellular mitochondrial transfer can be regarded as an extension of intracellular mitochondrial movement or intercellular communication, which undoubtedly increases the mtDNA content of the recipient cells and restores the respiration and survival of the recipient cells. (biomedcentral.com)

qRT-PCR Validation Urinary sediment total RNAs from 54 T1D sufferers were utilized to validate the results of the transcriptomic study. (tam-receptor.com)

Here, we report a surface enhanced Raman scattering technology for rapid and label-free exosomal detection (Exo-SERS) to aid in the discrimination of different cancer cells based on specific Raman phenotypes and multivariate statistical analysis. (mdpi.com)

This paper reviews intercellular spontaneous mitochondrial transfer modes, mechanisms, and the latest methods of AMT/T. Furthermore, potential application value and mechanism of AMT/T in disease treatment are also discussed. (biomedcentral.com)
Collectively, although scientists are increasingly aware of mitochondria's dynamic and transferable capabilities of mitochondria, the unclear mechanism and effectiveness of mitochondrial transfer/transplantation therapy limit its widespread application in diseases. (biomedcentral.com)
Objective To investigate the role and molecular mechanism of exosomal miR-224-5p in colorectal cancer (CRC). (besjournal.com)

In addition, we also discuss the existing methods of artificial mitochondrial transfer/transplantation (AMT/T) and their therapeutic effects on diseases to explore the potential applications of mitochondrial transfer and transplantation in the treatment of mitochondrial-related diseases, mitochondrial diseases, and cancer. (biomedcentral.com)

In addition, these therapies carry particular risks that may make them undesirable for many depressed patients (Andrade et al. (studylib.ru)

We hypothesized that the non-coding RNAs transcript may serve as susceptibility to disease biomarkers by smoking and vaping. (researchsquare.com)

while the stimulation of EV release increased the intercellular transfer of prions. (izon.com)
Recently developed imaging techniques provide direct evidence of cellular phenotypic change as a consequence of intercellular transfer of genetic materials mediated by EVs. (biomedcentral.com)

Activating mutations in the Leucine Rich Repeat Kinase 2 (LRRK2) cause Parkinson's disease and previously we showed that activated LRRK2 phosphorylates a subset of Rab GTPases (Steger et al. (stanford.edu)

Both prokaryotic and eukaryotic cells are known to release RNA, and this release can be passive or active. (wikipedia.org)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)
In the field of stem cells, cumulative evidence has shown that mitochondrial transfer contributes to stem cell-triggered repair of damaged cells [ 9 ]. (biomedcentral.com)
Our data suggested that exosomal ERp44 derived from ER-stressed NPC cells took an inevitable role in NPC chemoresistance and might act as a treatment target. (biomedcentral.com)

Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods. (norgenbiotek.com)

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. (norgenbiotek.com)

High quality, purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. (norgenbiotek.com)

Using a combination of parabiosis and serum transfer experiments, we find evidence supporting the hypothesis that Cre may be trafficked from its cell of origin to the epididymis through the circulatory system. (elifesciences.org)

Plasma/Serum RNA Purification Kits (Cat. (norgenbiotek.com)

According to research on intercellular mitochondrial transfer in physiological and pathological environments, mitochondrial transfer hold great potential for maintaining body homeostasis and regulating pathological processes. (biomedcentral.com)

Based on the nascent literature on mitochondrial transfer, we address the modes of mitochondrial transfer, their mechanisms, and their roles in physiological and pathological conditions. (biomedcentral.com)

This study indicated that the exosomal miRNA from patients with different condition of HFMD express unique miRNA profiles. (biomedcentral.com)

8] Hutvagner G., McLachlan J., Pasquinelli A.E., Balint E., Tuschl T., and Zamore P.D., A cellular function for the rnainterference enzyme dicer in the maturation of the let-7 small temporal rna. (edu.pl)

Further investigation is required to validate the functions and associations of these RNAs. (jcancer.org)

Anatomy15

Organisms15

Diseases4

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology1

Phenomena and Processes83

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science6

Health Care4

Exosomes15

Proteins4

NcRNAs6

MicroRNAs3

Extracellular16

LncRNAs1

Messenger RNA3

CircRNAs5

Pancreatic cancer1

Secretion3

MiRNAs9

Protein4

Tumor2

MRNA1

Molecules2

MiRNA expression2

Polymerase1

Expression2

Gene3

Exosome1

Cisplatin resistance1

Vesicles4

Intracellular2

Utilized to validate1

Detection1

Mechanism3

Therapeutic1

Therapies1

Biomarkers1

Intercellular transfer2

Subset1

Cells4

Methods1

EDTA1

Downstream1

Cell1

Serum1

Environments1

Pathological1

Study1

Small1

Validate1