AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Chromatography, Ion ExchangeChromatography, High Pressure LiquidChromatography, GelChromatography, AffinityChromatographyAnion Exchange ResinsGelsMolecular WeightChromatography, GasChromatography, LiquidElectrophoresis, Polyacrylamide GelChromatography, Thin LayerChromatography, DEAE-CelluloseAmino Acid SequenceMolecular Sequence DataKineticsAmino AcidsHydrogen-Ion ConcentrationMass SpectrometryChromatography, AgaroseSubstrate SpecificityCattleIsoelectric FocusingIsoelectric PointCarbohydratesGas Chromatography-Mass SpectrometryPeptide FragmentsTrypsinChemical FractionationOligosaccharidesEscherichia coliTemperatureRabbitsCarbohydrate SequenceSpectrophotometry, UltravioletElectrophoresis, Gel, Two-DimensionalRecombinant ProteinsProtein BindingMacromolecular SubstancesHydrolysisBase SequencePeptidesLiverChromatography, Reverse-PhaseChemistryMagnetic Resonance SpectroscopyChemical PhenomenaAmmonium SulfateImmunodiffusionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTandem Mass SpectrometryCloning, MolecularChromatography, PaperDrug StabilitySpectrometry, Mass, Electrospray IonizationIsoenzymesGlycoproteinsSequence Homology, Amino AcidCarbohydrate ConformationProteinsCell LineEnzyme StabilityBinding SitesIndicators and ReagentsMethodsAgaricalesLectinsCarrier ProteinsSepharosePolysaccharidesSolubilityHot TemperatureElectrophoresis, Agar GelDeuterium Exchange MeasurementFruiting Bodies, FungalGlycosaminoglycansBacterial ProteinsCentrifugation, Density GradientProtein ConformationSwineProteomicsGlycopeptidesBiological AssayGlycoside HydrolasesCytosolPulmonary Gas ExchangePlant LectinsRadioimmunoassayCell MembraneProteoglycansCyanogen BromideBlotting, WesternSulfatesBlood ProteinsPeptide MappingPeptide HydrolasesGalactoseExchange Transfusion, Whole BloodCells, CulturedTritium
AffinitySize-exclusion ChroFiltration chromatographyMembraneMass spectrometryCation-exchangeHigh-performance LiConcentrationNucleic acidsCharacterizationPermeation chromatographyColumn ChromatographySoluble proteinsAgaroseSeparationAmmonium sulfate preciElectrophoresisRecombinant ProteinSeparate proteinsFPLCPeptidesHPLCEscherichiaPolyacrylamide GelPeptideEnzymeMoleculesAggregatesAnionPhosphotransferase systemBacterialBiologicalHydrophobicCrystallographyInteractionsHomogeneityAminoIntracellularProteomicsFunctionalCysteine-richFractionsMoleculeDisulfidePurification StrategiesDetectionInteractionSolubilityExpressionBindTherapeuticMethodsWorkflowMolecular weightsPreparationBiochemicalMixtures
Affinity14
Size-exclusion Chro2
Filtration chromatography2
Membrane36
  • How Do Detergents Solubilize Membrane Proteins? (sigmaaldrich.com)
  • Common applications include cell lysis, solubilization of membrane proteins and lipids, protein crystallization, and reduction of background staining in blotting experiments. (sigmaaldrich.com)
  • Even though studying membrane proteins is a major challenge in protein biochemistry, they remain an important area of study due to their significant biological and pharmacological relevance. (sigmaaldrich.com)
  • Understanding the structure and function of membrane proteins requires their careful isolation in the native form in a highly purified state. (sigmaaldrich.com)
  • 1 These integral membrane proteins (IMPs) ( Figure 2 ) are not soluble in aqueous solutions as they aggregate to protect their hydrophobic domains, but are soluble in detergent solutions as micelles formed by detergents are analogous to the bilayers of the biological membranes. (sigmaaldrich.com)
  • Hydrophobic regions of membrane proteins, normally embedded in the membrane lipid bilayer, are now surrounded by a layer of detergent molecules and the hydrophilic regions are exposed to the aqueous medium. (sigmaaldrich.com)
  • This keeps the membrane proteins in solution. (sigmaaldrich.com)
  • Complete removal of detergent could result in aggregation due to the clustering of hydrophobic regions and, hence, may cause precipitation of membrane proteins. (sigmaaldrich.com)
  • Membrane solubilization by detergents can be described as a three stage process where the detergent-lipid-protein ratio is an important factor ( Figure 3 ). (sigmaaldrich.com)
  • Excess detergent is normally employed in solubilization of membrane proteins to ensure complete dissolution of the membrane and provide a large number of single protein molecule containing micelles. (sigmaaldrich.com)
  • However, for further physicochemical and biochemical characterization of membrane proteins, it is often necessary to remove the unbound detergent. (sigmaaldrich.com)
  • Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
  • Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)
  • The SNARE complex assembles from the vesicular protein Synaptobrevin-2 as well as SNAP25 (synaptosome-associated protein of 25 kDa) and Syntaxin-1, which are both anchored to the presynaptic membrane 2 . (nature.com)
  • Fusion of synaptic vesicles with the presynaptic membrane occurs in the presence of the NSF (N-ethylmaleimide-sensitive-factor) / α-SNAP (α-soluble NSF attachment protein) disassembly machinery. (nature.com)
  • These centrifugal devices feature a high-flux polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. (goldenbiotechnologies.com)
  • The vertical design of the membrane allows high-flux rates, low non-specific binding, and negligible protein aggregation on the membrane, giving reliable and consistent results for processing sample volumes between 0.5 and 100 mL. (goldenbiotechnologies.com)
  • Separation of different sized molecules Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. (goldenbiotechnologies.com)
  • To extract membrane proteins, detergents that solubilize membrane proteins (membrane protein solubilizers) are used. (fujifilm.com)
  • This page shows how to solubilize membrane proteins with products from Cytiva. (sigmaaldrich.com)
  • leadXpro is a team of experts in membrane protein science and a rapidly growing biotech company. (myscience.uk)
  • We have an integrated platform for membrane protein biochemistry, biophysics and structural biology, including X-ray crystallography and cryo-EM. (myscience.uk)
  • You will perform membrane protein biochemistry on ion channels, transporters and GPCRs to provide high quality samples for structural and biophysical studies. (myscience.uk)
  • Contribution to scientific research on membrane proteins for drug discovery within an experienced team. (myscience.uk)
  • Ultrafiltration renaturation: select a membrane with a suitable molecular weight retention to allow denaturant to pass through the membrane and prevent protein from passing through. (medicilon.com)
  • Soluble proteins released by vigorous homogenization of cells with glass beads are shown to be necessary for the phosphoenolpyruvate-dependent phosphorylation of sucrose in combination with one or more proteins that remain tightly associated with the membrane fraction. (ox.ac.uk)
  • Membrane proteins play a key role in the cell's energy metabolism and in its signalling and communication with its environment. (lu.se)
  • More than half of all drugs that are in use today target membrane proteins. (lu.se)
  • The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)
  • The lectures address the three different main types of membrane proteins and associated cellular processes: transport and transporters, signal transduction and receptors, bioenergetics and photosynthetic and respiratory proteins. (lu.se)
  • Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
  • This is followed by experimental determination using genetic construction and expression of a fusion protein of the membrane protein and a marker protein in a bacterial system which is subsequently analysed. (lu.se)
  • In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)
  • Group discussions about e.g. the similarities/dissimilarities, cloning and overexpression strategies, and structure and function of membrane proteins. (lu.se)
  • An individually planned and executed minor project during two weeks, in which the students express a membrane protein of their choice and demonstrate in some way that the expression was successful. (lu.se)
  • The project entails practice in literature searching, project planning and documentation, and provides specialised practical knowledge of expression and management of membrane proteins. (lu.se)
Mass spectrometry7
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
  • Analytical techniques, for example chromatography and mass spectrometry, along with various tools such as cloning, CRISPR/Cas, single-use equipment, machine learning [and] use of advanced sensors and automation have helped in the advancement of biopharma research," said Dr. Johannes Buyel , head of the department of Bioprocess Engineering at the Fraunhofer IME and associate professor at the RWTH Aachen University. (technologynetworks.com)
  • The overall goal of Core C, the Protein Characterization Core is to provide mass spectrometry, HPLC, and peptide synthesis services for SUPERFUND investigators. (nih.gov)
  • Proteins separated by SDS-PAGE or other methods can be identified by mass spectrometry . (fujifilm.com)
  • The peptide fragments are then recovered from the gel and subjected to mass spectrometry (LC-MS/MS or MALDI-TOF MS) to determine the mass of each fragment. (fujifilm.com)
  • Avantor has the resources to make your Chromatography or Mass Spectrometry applications run efficiently and effectively-from the measuring apparatus needed for chromatography, or the proteins used to fulfill sample manipulation during mass spectrometry. (vwr.com)
  • If the coding sequence of the protein is unknown but the protein is purified, mass spectrometry (or chemical methods such as Edman degradation) can be used to determine the amino acid sequence of the protein, which can then be used to search for the the corresponding DNA sequence. (hideyuki-ts.com)
Cation-exchange1
High-performance Li1
Concentration9
  • 5 A further increase of detergent concentration causes progressive delipidation of the lipid-protein-detergent mixed micelles. (sigmaaldrich.com)
  • Plasma has a significant dynamic range, with more than 10 orders of magnitude separating albumin concentration and the rarest measurable proteins identified to date [ 10 ]. (hindawi.com)
  • Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. (cshlpress.com)
  • For protein and other biological samples, such as nucleic acids, with a 30K molecular-weight cutoff (MWCO), Pierce Protein Concentrators PES offer rapid protein concentration, excellent sample recovery, and instrument compatibility. (goldenbiotechnologies.com)
  • Versatile -easily perform sample concentration, diafiltration, or buffer-exchange of biological samples For easy identification, the MWCO value is etched on the side of each device. (goldenbiotechnologies.com)
  • Protein recovery will vary depending on the specific protein in the sample and its starting concentration. (goldenbiotechnologies.com)
  • To achieve >90% recovery of protein with the 0.5 mL concentrators, the minimum protein sample concentration should be 0.05 mg/mL. (goldenbiotechnologies.com)
  • For all other concentrators, the minimum protein sample concentration should be 0.1 mg/mL. (goldenbiotechnologies.com)
  • Dilute protein concentration: high concentration is easy to form aggregates (lower renaturation yield). (medicilon.com)
Nucleic acids1
  • GFC is used to separate water-soluble high polymers such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and aqueous mobile phase. (shimadzu.com)
Characterization3
  • Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
  • Our team combines specialized knowledge in protein science, pioneering technologies in structural biology and expertise in ligand design and characterization. (myscience.uk)
  • Resolution of the phosphotransferase enzymes of Streptococcus mutans: purification and preliminary characterization of a heat-stable phosphocarrier protein. (ox.ac.uk)
Permeation chromatography2
Column Chromatography3
Soluble proteins2
  • Like many Chinese medicinal herbs, licorice root has high content of soluble proteins, some of which are glycated and remain soluble even in the boiling decoction. (springer.com)
  • Co-expression of molecular chaperones is used to increase the proportion of soluble proteins. (medicilon.com)
Agarose2
  • Final purification was achieved by gel filtration on 2 or 4% agarose or ion exchange chromatography on diethylaminoethyl cellulose. (elsevierpure.com)
  • Comparable amounts of highly purified protein were obtained from normal and hemophilic plasma following the agarose chromatography step. (elsevierpure.com)
Separation5
  • At its core, chromatography is the interaction between a mobile phase (which carries the mixture being separated) and a stationary phase (which performs the separation). (coleparmer.com)
  • Today, most applications of chromatography are generally less colorful but the underlying principle of separation remains unchanged. (coleparmer.com)
  • Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. (shimadzu.com)
  • Fujifilm Wako offers standard reagents such as protein extraction reagents and SDS-PAGE reagents, as well as unique reagents such as "Phos-tag™" products for separation and detection of phosphoproteins. (fujifilm.com)
  • Only when a suitable renaturation buffer is selected to make the protein fold correctly, can further chromatographic separation be successfully completed. (medicilon.com)
Ammonium sulfate preci2
Electrophoresis4
  • Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. (cshlpress.com)
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
  • Protein electrophoresis is an effective method to separate specific proteins from a protein mixture. (fujifilm.com)
  • However, when reduced by β mercaptoethanol and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, a single subunit species with an estimated 195,000 molecular weight was found for both normal and hemophilic factor VIII. (elsevierpure.com)
Recombinant Protein8
  • Buyel's work focuses on recombinant protein expression, overall bioprocess integration, the modeling of manufacturing processes and their digitalization. (technologynetworks.com)
  • Recombinant protein expression and purification workflows depend on many variables. (sigmaaldrich.com)
  • Significant advancements have been made in the area of recombinant protein expression and purification methodology along with a plethora of commercially available systems and kits. (sigmaaldrich.com)
  • Recombinant protein biochemical or biological activity is partially determined by discrete domains within the protein, of which are often reliant on the protein being folded into secondary, tertiary, and quaternary structures. (sigmaaldrich.com)
  • When the rare tRNAs are depleted to produce the recombinant protein, proliferation of the host cells is restricted, leading to low yield. (hideyuki-ts.com)
  • The environment of the recombinant protein: When the fermentation temperature is high (37-42℃) or the intracellular pH is close to the isoelectric point of the protein, inclusion bodies are easily formed. (medicilon.com)
  • The recombinant protein is a heterologous protein of Escherichia coli . (medicilon.com)
  • Protein renaturation is the most critical and complex issue in recombinant protein purification . (medicilon.com)
Separate proteins3
FPLC1
Peptides3
HPLC1
  • Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. (shimadzu.com)
Escherichia1
Polyacrylamide Gel1
  • In SDS-PAGE, proteins are given negative charges by addition of sodium dodecyl sulfate (SDS) and then electrophoresed on a polyacrylamide gel. (fujifilm.com)
Peptide3
Enzyme4
  • We have partially purified phosphotransferase enzyme I and have purified a heat-stable phosphocarrier protein (HPr) to apparent homogeneity, by gel filtration and ion-exchange chromatography from the soluble fraction. (ox.ac.uk)
  • Furthermore, it appears to be partially complexed with a heat-stable enzyme III-like protein in cell-free fractions from S. mutans, and we also report the purification of this complex. (ox.ac.uk)
  • Enzyme I from S. mutans is a protein (native Mr greater than 100,000) that cross-complements enzyme I from S. aureus. (ox.ac.uk)
  • Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)
Molecules5
  • At concentrations equal to, or higher than the detergent's CMC, the lipid bilayer becomes saturated with detergent molecules and breaks apart generating lipid-protein-detergent mixed micelles (c). 8 A detergent/protein ratio of around 1-2 (w/w) is believed to be sufficient to solubilize IMPs to form lipid-protein-detergent mixed micelles. (sigmaaldrich.com)
  • These proteins can be present in a cell as single molecules (monomers) or as a group of several chaperone molecules (oligomers). (elifesciences.org)
  • When separating out molecules or proteins of different charges at a certain pH. (flashcardmachine.com)
  • Due to their three-dimensional structures, proteins can bind specifically to certain molecules, called ligands. (fujifilm.com)
  • It can be used for downstream purification of large molecules such as high molecular weight (HMW) plasma proteins IgA and IgM, viruses, VLPs, and PEGylated proteins. (bio-rad.com)
Aggregates2
  • The ephedrine alkaloid-containing colloidal nanoparticles discovered in another licorice containing Chinese medicinal decoction [ 12 ] imply that licorice root proteins would hypothetically interact with aconitine to form aggregates thereafter affecting the toxicity. (springer.com)
  • Insoluble protein aggregates associated with Alzheimer's and Prion disease. (flashcardmachine.com)
Anion3
  • Oligosaccharide fingerprinting employing high performance anion exchange liquid chromatography with pulsed amperometric detection (HPAE-PAD). (chipsbooks.com)
  • Nuvia HP-Q Anion Exchange Resin was introduced to fill customers' need for a high-performance resin compatible with high-throughput (HTP) large biomolecule purification. (bio-rad.com)
  • To learn more about Nuvia HP-Q Anion Exchange Resin, please visit bio-rad.com/Nuvia HP-Q . (bio-rad.com)
Phosphotransferase system1
  • It is concluded that fructose is transported into A. radiobacter and related organisms via a periplasmic fructose/mannose-binding-protein-dependent active-transport system, in contrast to the phosphotransferase system used by many other species of bacteria. (microbiologyresearch.org)
Bacterial1
  • These variables include but are not limited to the physical properties and biological function of the protein, and whether a bacterial or eukaryotic cell line should be used to express the protein of interest. (sigmaaldrich.com)
Biological8
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • Understanding how proteins function is an essential part of many biological research endeavors. (cshlpress.com)
  • When exposed to biological medium or multiple composition dispersions like herbal decoction, protein corona formed on the nanoparticles (NPs) diversely alters the biological fate and pharmaceutical efficacy of particles [ 10 ]. (springer.com)
  • One representative experiment of two biological replicates using different protein lots in separate assays is shown. (elifesciences.org)
  • Easily and quickly concentrate 30K MWCO protein and biological samples with Thermo Scientific Pierce Protein Concentrators PES (polyethersulfone), which are disposable centrifugation devices that enable fast sample recovery and a short processing time. (goldenbiotechnologies.com)
  • Proteins have diverse functions in biological phenomena such as catalysis for biological reactions, response to stimuli, and molecular transport. (fujifilm.com)
  • Proteins make up about 15% of the components of cells and play a central role in biological phenomena. (fujifilm.com)
  • Numerous protein purification methods are widely used in both biological and biomedical research. (sigmaaldrich.com)
Hydrophobic3
  • Proteins are held in the lipid bilayer by hydrophobic interactions between the lipid tails and hydrophobic protein domains. (sigmaaldrich.com)
  • 2 Proteins are incorporated into these micelles via hydrophobic interactions. (sigmaaldrich.com)
  • Protein-ligand binding occurs primarily through relatively weak bonds such as hydrogen bonds, van der Waals forces, and hydrophobic interactions. (fujifilm.com)
Crystallography3
  • Now, high-resolution pump-probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. (nature.com)
  • Outline an expression and purification strategy, using both traditional and high-throughput methods, to produce a sample suitable for protein crystallography. (hideyuki-ts.com)
  • For crystallography studies, a protein sample with high purity, solubility and yield must be obtained. (hideyuki-ts.com)
Interactions4
  • The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. (cshlpress.com)
  • It has been known that liquiritin from licorice forms complexation with aconitine and therefore reduces the amount of free aconitine [ 7 ], indicating that interactions between aconitine with other major amphiphilic compounds (i.e., protein) from licorice may facilitate the formation of aconitine complex of such kind, too. (springer.com)
  • For this reason, this study aims to clone, express, purify, and characterize Dengue ns5 and its interactions with other Dengue proteins. (umsystem.edu)
  • pH should not affect folded protein stability and stabilize interactions. (flashcardmachine.com)
Homogeneity1
  • Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. (cshlpress.com)
Amino8
Intracellular2
  • The intracellular protein extracts of a fungal strain Pycnoporus sanguineus was used as reducing and stabilizing agents to synthesize AuNPs with various shapes and dimensions, which present good catalyzing ability on the degradation of 4-nitroaniline [ 11 ]. (springer.com)
  • Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)
Proteomics2
  • The issue of abundant (often housekeeping) proteins masking regulatory proteins of lower abundance (such as signaling proteins and cytokines) continues to be a challenging issue for proteomics particularly in the case of biofluids. (hindawi.com)
  • While modern proteomics experiments permit the analysis of hundreds to thousands of proteins in complex samples, the most powerful use of this data would combine information on protein activity with the identities of the active proteins. (hindawi.com)
Functional3
  • Structural prediction of sugar-binding proteins functional in chemotaxis and transport. (microbiologyresearch.org)
  • To understand a protein, it is necessary to know what kind of protein it is (identification), what kind of structure it has (structural analysis), and what kind of function it has (functional analysis). (fujifilm.com)
  • A number of proteins from each process, for which the structure is known, are explored in greater detail in order to highlight the functional molecular mechanisms. (lu.se)
Cysteine-rich2
  • This toxin belongs to the cysteine-rich secretory protein (CRISP) family. (wikipedia.org)
  • Ophanin is a cysteine-rich secretory protein and therefore belongs to the CRISP family. (wikipedia.org)
Fractions2
  • Migration of THP-1 monocytes in response to complete or fractionated platelet releasate was assessed and located to just one of the forty-nine ion-exchange fractions. (hindawi.com)
  • Proteins identified within the fraction with monocyte promigratory activity and not in other inactive fractions included vimentin, PEDF, and TIMP-1. (hindawi.com)
Molecule1
  • The experiments show that oligomers form when the interdomain linker of one BiP molecule is bound to the region of an adjacent BiP molecule that is normally reserved for binding to unfolded proteins. (elifesciences.org)
Disulfide5
  • These proteins possess 16 strictly conserved cysteines and contain 8 disulfide bonds. (wikipedia.org)
  • Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)
  • Describe the basice disulfide exchange mechanism. (flashcardmachine.com)
  • The expression and purification strategies depend on the characteristics of the protein of interest, such as size, shape, intrinsic solubility, requirement of post-translational modifications and/or cofactors, presence of disulfide bridges, in vitro stability. (hideyuki-ts.com)
  • The expression level is too high: the reason may be that the synthesis speed is too fast, so that there is not enough time to fold, the disulfide bonds cannot be paired correctly, too many non-specific bindings between proteins, and the protein cannot achieve sufficient solubility, etc. (medicilon.com)
Purification Strategies2
  • However, proteins are complex macromolecules, and optimal protein expression and purification strategies must be determined empirically. (sigmaaldrich.com)
  • The structure of some homologous proteins might have been solved previously and we can refer to their expression and purification strategies, which might prevent some waste of time on trial-and-errors. (hideyuki-ts.com)
Detection1
Interaction1
  • These proteins may modulate the interaction of platelets with their local cellular environment. (hindawi.com)
Solubility2
  • Additionally, protein solubility is a highly desirable attribute for successful protein purification and is influenced by numerous factors including, size and N- and C-terminal elements. (sigmaaldrich.com)
  • We might fail in crystallising the single full-length protein (often because of low solubility). (hideyuki-ts.com)
Expression7
  • MP increased the mRNA expression of T-bet and the protein expression of Bcl-2 in the spleen and decreased the protein expression of caspase-3 and Bax. (hindawi.com)
  • The Wadsworth Center Protein Expression Core is available to help generate the protein of interest and can assist in many aspects of protein expression and/or purification. (wadsworth.org)
  • In order to request service from the Protein Expression Core, please contact Dr. Karen Chave , Director. (wadsworth.org)
  • We spent more than 4 months trying to optimize conditions for our prokaryotic expression prior to using the Protein Expression Core services. (wadsworth.org)
  • 5). Protein expression system a. (ntu.edu.tw)
  • If the gene encoding a target protein is already known, the protein can be produced in cells by introducing a protein expression vector containing a cDNA of the target protein. (fujifilm.com)
  • Inclusion bodies have nothing to do with the type of protein and expression system, only the result of protein overexpression. (medicilon.com)
Bind3
Therapeutic1
  • Thus, altered or deregulated platelet function underpins many diseases, and platelet proteins are potential targets for novel therapeutic agents. (hindawi.com)
Methods6
  • Most chromatography methods have an inert mobile phase that carries the analyte through a long stationary phase housed inside a column. (coleparmer.com)
  • Protein-based methods. (chipsbooks.com)
  • Gel-based methods. (chipsbooks.com)
  • The methods commonly used in protein research are described below. (fujifilm.com)
  • Whether researchers aim to study protein function or seek to scale up protein purification using strategies for downstream, industrial-scale biologics and pharmaceutical production, there are numerous protein purification methods, reagents, and tools available. (sigmaaldrich.com)
  • Determination of the transmembrane topology of a protein starts with a model of the protein based on sequence information and theoretical methods. (lu.se)
Workflow1
  • The selected protein purification method will partially determine the sample preparation workflow. (sigmaaldrich.com)
Molecular weights1
Preparation1
Biochemical2
  • Over 300 proteins were identified in the releasate, with a wide range of annotated biophysical and biochemical properties, in particular platelet activation, adhesion, and wound healing. (hindawi.com)
  • used biochemical techniques to study how an Hsp70 protein in the endoplasmic reticulum called BiP forms oligomers. (elifesciences.org)
Mixtures1
  • Chromatography is a technique for separating mixtures into their constituent parts. (coleparmer.com)