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Chromatography, Ion ExchangeIon Exchange ResinsChromatography, High Pressure LiquidChromatography, GelIon ExchangeChromatography, AffinityChromatographyAnion Exchange ResinsMolecular WeightElectrophoresis, Polyacrylamide GelChromatography, GasChromatography, LiquidKineticsChromatography, Thin LayerAmino Acid SequenceAmino AcidsMolecular Sequence DataChromatography, DEAE-CelluloseHydrogen-Ion ConcentrationMass SpectrometrySubstrate SpecificityChromatography, AgaroseCattleCarbohydratesIsoelectric PointIsoelectric FocusingPeptide FragmentsTrypsinGas Chromatography-Mass SpectrometryDeuterium Exchange MeasurementOligosaccharidesChemical FractionationSpectrophotometry, UltravioletTemperatureCation Exchange ResinsRabbitsPulmonary Gas ExchangeProtein BindingEscherichia coliMacromolecular SubstancesMagnetic Resonance SpectroscopyPeptidesExchange Transfusion, Whole BloodRecombinant ProteinsChemistrySister Chromatid ExchangeHydrolysisChemical PhenomenaImmunodiffusionCarbohydrate SequenceLiverBase SequenceDrug StabilitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBinding SitesSpectrometry, Mass, Electrospray IonizationProtein ConformationAmmonium SulfateCloning, MolecularAgaricalesSequence Homology, Amino AcidGlycoproteinsSolubilityIon ChannelsCell LineFruiting Bodies, FungalCarrier ProteinsSodiumProteinsMethodsIsoenzymesChromatography, Reverse-PhaseCalciumGlycosaminoglycansTandem Mass SpectrometryEnzyme StabilityCell MembraneSulfatesHot TemperatureLectinsCyanogen BromideGlycoside HydrolasesCationsProteoglycansBacterial ProteinsErythrocytesCytosolMolecular StructureChromatography, PaperPolysaccharidesSwineGlycopeptidesBiological AssayMagnesiumHydrogenAdsorptionPlant LectinsIndicators and ReagentsCentrifugation, Density GradientAdenosine Triphosphate
Affinity ChromatographyResinsHigh Performance LiCationAdsorptionResinReverse-Phase ChromatoSeparationSeparatesProtein PurificationMoleculeDEAE-CelluloseBiomoleculesProteinsReagentsSeparationsElutionColumnsStationary phaseIonicLiquidOppositely chargedCationsInorganicElectrophoresisChromatographicEluteAmino acidSolventAqueousPurification methodRetentionTypes of ionSuitableSaltsMethodTechniqueAnalysisPhaseWeakApplicationsQuaternarySilica-basedReliableEfficiently
Affinity Chromatography10
Resins20
  • Bio-Rad manufactures a wide range of chromatography resins for process separations, providing process developers with great flexibility and high productivity. (bio-rad.com)
  • UNOsphere IEX Resins are produced in a controlled single-step polymerization using matrix, crosslinker, and functional monomers.These ion exchange resins have excellent pressure-flow properties and perform well in capture, intermediate, and polishing purification steps. (bio-rad.com)
  • Size exclusion chromatography and ion-exchange resins are used to separate sucrose from fructose and dextrose in the sugar industry and to separate lactose, salts, and non-protein nitrogen in the dairy industry. (eurodia.com)
  • size exclusion chromatography and ion-exchange resins are used to separate dextrose or maltose from molecules with a high degree of polymerization (sucrose, amino acids, salts). (eurodia.com)
  • Chromatography resins are available in three major types includes, synthetic, inorganic, and natural media. (prnewswire.co.uk)
  • Industry prefers synthetic and natural type media over inorganic chromatography resins. (prnewswire.co.uk)
  • The key drivers for the chromatography resins could be the heavy demand for monoclonal antibodies owing to a heighten number of critical diseases concluding in types of therapeutics. (prnewswire.co.uk)
  • Pharmaceuticals and biotechnology are the major end-users for chromatography resins and are estimated to continue with higher numbers. (prnewswire.co.uk)
  • Various opportunities for the chromatography resins market could be in the academic usage of drug recovery and genetic engineering. (prnewswire.co.uk)
  • The growth in terms of therapeutic antibodies demand, increasing utilization of separation & purifying techniques in the food industry, and emerging R&D activities in biopharmaceutical are the some of the factors contributing to the chromatography resins industry growth. (prnewswire.co.uk)
  • DuPont offers a full selection of high-quality strong acid cation and weak base anion ion exchange resins to tackle the challenging processing conditions that come with synthesizing organic and amino acids. (dupont.com)
  • Producing high quality acids with highly stable ion exchange resins. (dupont.com)
  • The dissolved citric and lactic acids are subsequently purified through a deashing step using ion exchange resins. (dupont.com)
  • The high acid loading causes excessive osmotic stress, which can break ion exchange resins. (dupont.com)
  • DuPont is an industry leading ion exchange resin manufacturer, delivering resins with physical stability and purification performance for your acid production needs. (dupont.com)
  • DuPont offers a wide variety of ion exchange resins for the recovery and purification of essential amino acids, including lysine, histidine, tryptophan, methionine and glutamine. (dupont.com)
  • DuPont™ Amberlite™ ion exchange resins resist breakage under high loading stress while maintaining purification performance. (dupont.com)
  • In organic acid fermentation, DuPont's acrylic chromatography resins can separate the organic acids from contaminating salts and residual dextrose with sharp and efficient elution peaks. (dupont.com)
  • DuPont chromatography resins quickly and efficiently elute the salts and non-anion compounds while retaining the anionic compounds. (dupont.com)
  • IEC separates amino acids based on their charge characteristics using ion exchange resins. (pharmiweb.com)
High Performance Li3
  • Aim: The present study is aimed to assess the precision and reproducibility of Ion Exchange Chromatography (IEC) resin method in comparison to High Performance Liquid Chromatography (HPLC) with respect to the estimation of Glycated Haemoglobin (HbA1c). (manipal.edu)
  • A theoretical description of analyte retention in high-performance liquid chromatography (HPLC) has been the subject of various publications. (springer.com)
  • Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. (springer.com)
Cation9
Adsorption6
  • The 2021 release of the GoSilico™ Chromatography Modeling Software has introduced a new adsorption isotherm class that is based on a colloidal description of proteins, the colloidal particle adsorption model (CPA). (pharmaceuticalonline.com)
  • Chromatography, ion exchange, and adsorption are similar technologies that involve specific uses of porous, calibrated, and functionalized polymer-resin beads. (eurodia.com)
  • Adsorption chromatography separates organic acids into salts, colors, and sugars. (eurodia.com)
  • An original process based on a combination of technologies (membrane filtration, electrodialysis, ion exchange, adsorption) to produce second-generation sugars. (eurodia.com)
  • There are several ways to model the separation mechanism: partition, adsorption, ion exchange, and size exclusion. (springer.com)
  • In this sense, the physicochemical characterisation of different industrial (technical) lignins are performed exploiting different methods, like Brunauer-Emmett-Teller N2 adsorption, particle size distribution, Fourier transform infrared spectroscopy, ultraviolet-visible absorption spectroscopy, gel permeation chromatography, and thermogravimetric analysis. (lu.se)
Resin6
  • Ion exchange is an extremely robust and adaptable technique allowing the extraction from a liquid of a charged molecule, which is then exchanged for an ion initially bound to the resin. (eurodia.com)
  • Chromatography makes it possible to separate the molecules in a mixture, according to their affinities with the resin used. (eurodia.com)
  • Hydrophilic polymer-based, exchange columns packed with non-porous resin particles are available for the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, DNA fragments, nucleic acids, oligo DNA, and siRNA. (tosohbioscience.com)
  • The global Chromatography Resin Market is estimated to grow at a decent rate in terms of value. (prnewswire.co.uk)
  • Lysine is usually recovered from the fermented broth of sugar molasses, but it requires a larger volume of ion exchange resin for production than any other amino acid. (dupont.com)
  • Our resin strength can match the most advanced continuous ion exchange process (CIX) with cost-effective manufacturing of lysine and other amino acids. (dupont.com)
Reverse-Phase Chromato2
Separation14
  • Starting from 1947, Spedding and Powell used displacement ion-exchange chromatography for the separation of the rare earths. (wikipedia.org)
  • Additionally, they showed the ion-exchange separation of 14N and 15N isotopes in ammonia. (wikipedia.org)
  • At the start of the 1950s, Kraus and Nelson demonstrated the use of many analytical methods for metal ions dependent on their separation of their chloride, fluoride, nitrate or sulfate complexes by anion chromatography. (wikipedia.org)
  • Ion-exchange is used for the analysis of small ions but the key application area of the technique is the separation of biomolecules such as peptides, proteins, and oligonucleotides. (galaklc.com)
  • Ion exchange chromatography is the most common separation mode for protein purification schemes. (tosohbioscience.com)
  • PROTE INDEX ™ Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. (marvelgent.com)
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
  • Novel ion exchange chromatography method for nca arsenic separation. (atomki.hu)
  • NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
  • For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
  • Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
  • Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
  • TFA is widely used as a mobile phase additive in the HPLC separation of biological molecules, such as proteins and peptides, because it acts as an ion-pairing reagent and equilibrates quickly so that it can be used with gradient elution. (wazeesupperclub.com)
  • Reversed-phase chromatography is the most common HPLC separation technique and is used for separating compounds that have hydrophobic moieties and do not have a dominant polar character (although polarity of a compound does not exclude the use of RP-HPLC). (wazeesupperclub.com)
Separates2
Protein Purification1
  • The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
Molecule1
  • The molecule is positively charged because the pH for chromatography is less than the pI (a/k/a pH(I)). In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. (wikipedia.org)
DEAE-Cellulose2
  • The isoform I of the glucoamylase produced by 15.1 strain was sequentially submitted to DEAE-Cellulose and CM-Cellulose chromatography, and purified 141-fold, with 5.45 percent recovery. (bvsalud.org)
  • An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. (bvsalud.org)
Biomolecules4
Proteins2
  • Chromatography is a code of techniques used to purify as well as separate bimolecular substances such as proteins. (prnewswire.co.uk)
  • In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)
Reagents9
  • The global market for chromatography reagents will grow from $8.3 billion in 2014 to nearly $11 billion by 2019 at a compound annual growth rate (CAGR) of 5.2% between 2014 and 2019. (bccresearch.com)
  • An overview of the global market for reagents for chromatography. (bccresearch.com)
  • Analysis on the market size, market leaders, and factors affecting the market of chromatography reagents from the perspective of its end users, which includes the pharma and biotech, food and beverages, environmental agencies and other sectors. (bccresearch.com)
  • Comparisons, usage statistics, and the advantages and disadvantages of different types of chromatography products and reagents. (bccresearch.com)
  • The scope of this study encompasses multiple technologies and procedures related to chromatography reagents. (bccresearch.com)
  • The report examines the overall chromatography reagents industry and the types of technologies currently in use as well as future technological developments, end-user markets and regional distribution, in addition to regulatory influences, patents filed and pipeline products that affect product distribution. (bccresearch.com)
  • Expensive ion pair reagents are not required in HILIC, and it can be conveniently coupled to mass spectrometry (MS), especially in the electrospray ionization (ESI) mode. (springer.com)
  • How do ion pairing reagents work? (wazeesupperclub.com)
  • The ionic samples form an ion-pair with ion-pair reagents in the mobile phase to become electrically neutral. (wazeesupperclub.com)
Separations2
  • Automatic in-line detection was progressively introduced from 1960 to 1980 as well as novel chromatographic methods for metal ion separations. (wikipedia.org)
  • Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. (fishersci.com)
Elution1
  • Another advantage of ion exchange is the predictability of elution patterns (based on the presence of the ionizable group). (wikipedia.org)
Columns7
  • A challenge that needs to be overcome in the future development of IC is the preparation of highly efficient monolithic ion-exchange columns and overcoming this challenge would be of great importance to the development of IC. (wikipedia.org)
  • Limited water consumption, due to the special care taken in designing our ion-exchange columns. (eurodia.com)
  • Polystyrene-based ion exchange columns are available for analyzing low MW sugars, amino acids, nucleic acid bases, and small drug candidates. (tosohbioscience.com)
  • Specialty columns are available for organic acids by ion-exclusion (OApak-A and SCX) and mono- and disaccharides (Sugar AXI and Sugar AXG). (tosohbioscience.com)
  • These quaternary amine strong anion-exchange columns are highly stable for aqueous and low pH mobile phases. (fishersci.com)
  • Based on high purity silica bonded with an ion-exchange ligand, Hypersil GOLD SAX HPLC columns provide outstanding peak shape and sensitivity. (fishersci.com)
  • Ion Exchange PhyTip ® columns is used for efficient parallel purification of small protein samples. (biotage.com)
Stationary phase6
  • Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it. (wikipedia.org)
  • The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. (springer.com)
  • In normal phase liquid chromatography (NP-LC), the stationary phase is more polar than the mobile phase. (springer.com)
  • In ion pair chromatography, the solute ion is distributed between the mobile and the stationary phase together with an ion of opposite charge (a so-called counterion). (wazeesupperclub.com)
  • Reversed-phase chromatography is a technique using alkyl chains covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase, which has a stronger affinity for hydrophobic or less polar compounds. (wazeesupperclub.com)
  • The increase in hydrophobic character of the ion-pair results in a greater affinity for the reverse stationary phase and leads to sample resolution. (wazeesupperclub.com)
Ionic4
  • Ion-exchange phases separate solutes on the basis of differences in ionic charge. (galaklc.com)
  • Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. (galaklc.com)
  • For the former, ionic species are attached to the silica surface, whereas for the latter the ion-exchange groups are distributed throughout the matrix. (galaklc.com)
  • Ion-pairing agents are compounds that contain both an ionic functional group and a hydrophobic portion, such as a hydrocarbon chain. (wazeesupperclub.com)
Liquid7
  • During my college study, I found liquid chromatography to be a profound subject. (galaklc.com)
  • Corn, soybean meal, and isolated soybean protein samples were acid-hydrolyzed for amino acid content by reverse phase liquid chromatography (LC) and by conventional ion-exchange chromatography (IEC) using an amino acid analyzer. (psu.edu)
  • Elkin, RG 1984, ' Quantitative amino acid analysis of feedstuff hydrolysates by reverse phase liquid chromatography and conventional ion-exchange chromatography ', Journal of the Association of Official Analytical Chemists , vol. 67, no. 5, pp. 1024-1026. (psu.edu)
  • After derivatization, the amino acids are separated using liquid chromatography. (pharmiweb.com)
  • Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
  • Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. (springer.com)
  • Normal or reversed-phase liquid chromatography can be used for analysis. (springer.com)
Oppositely charged2
  • Ion Exchange chromatography (IEC) is a technique based on the difference in the strength of the interaction between a sample ion and an oppositely charged functional group on the support. (tosohbioscience.com)
  • Ion pairing describes the (partial) association of oppositely charged ions in electrolyte solutions to form distinct chemical species called ion pairs. (wazeesupperclub.com)
Cations3
Inorganic1
  • Weak ion-exchangers are used for the analysis of inorganic ions, a technique more specifically termed ion chromatography. (galaklc.com)
Electrophoresis1
  • Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. (cshlpress.com)
Chromatographic1
  • Modeling the impact of holdup volume from chromatographic workstations on ion-exchange chromatography , Industrial and Engineering Chemistry Research 61,28 (2022): 10195-10204. (cadet-web.de)
Elute1
  • For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
Amino acid1
Solvent1
Aqueous1
Purification method2
Retention2
  • In the schematics below, the ion strength of the counterions can be adjusted to shift the equilibrium position and thus the retention times of the analytes. (galaklc.com)
  • The technique is often used in reversed-phase chromatography as a convenient method to control the retention of solutes. (wazeesupperclub.com)
Types of ion1
Suitable1
Salts1
  • They performed ion methods to treat clays with the salts, resulting in the extraction of ammonia in addition to the release of calcium. (wikipedia.org)
Method3
Technique1
  • However, there are also disadvantages involved when performing ion-exchange chromatography, such as constant evolution with the technique which leads to the inconsistency from column to column. (wikipedia.org)
Analysis3
Phase2
Weak2
  • Weak ion-exchangers often provide slightly different selectivity from strong exchangers. (tosohbioscience.com)
  • Others form weak ion exchangers, where the degree of dissociation, and thus the exchange capacity, varies markedly with pH. (marvelgent.com)
Applications2
Quaternary1
  • Sulfonic and quaternary amines form strong ion exchangers, which are completely ionized over a broad pH range. (marvelgent.com)
Silica-based1
  • Both silica-based and polymer-based ion-exchangers are available. (galaklc.com)
Reliable1
  • Marvelgent ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography. (marvelgent.com)
Efficiently1
  • Our advanced chromatography systems are designed to efficiently isolate and purify charged analytes to meet the exact specifications of your application. (sheng-han.net)