Loading...
  • buffer
  • Desalting and buffer exchange both entail recovering the components of a sample in whatever buffer is used to pre-equilibrate the small, porous polymer beads (resin). (wikipedia.org)
  • Desalting occurs when buffer salts and other small molecules are removed from a sample in exchange for water (with the resin being pre-equilibrated in water). (wikipedia.org)
  • Buffer exchange occurs when the buffer salts in a sample are exchanged for those in another buffer. (wikipedia.org)
  • The macromolecular components are recovered in the buffer used to pre-equilibrate the gel-filtration matrix, while the small molecules can be collected in a later fraction volume or be left trapped in the resin. (wikipedia.org)
  • There are a number of common formats for performing gel filtration for smaller (less than 4mL) volumes: Chromatography columns Gravity-flow columns Chromatography cartridges Centrifuge columns Centrifuge plates Gravity-flow, or drip, columns use head-pressure from a buffer-chase to push the sample through the gel filtration matrix. (wikipedia.org)
  • If the pH of the buffer is below the pI of the protein being run, the protein will migrate to the negative pole of the gel (positive charge is attracted to the negative pole). (wikipedia.org)
  • In the most common FPLC strategy, ion exchange, a resin is chosen that the protein of interest will bind to the resin by a charge interaction while in buffer A (the running buffer) but become dissociated and return to solution in buffer B (the elution buffer). (wikipedia.org)
  • column
  • For gel filtration applications it is important to select a column size and format that is suitable for your sample. (wikipedia.org)
  • The mobile phase moves through the chromatography column (the stationary phase) where the sample interacts with the stationary phase and is separated. (wikipedia.org)
  • different
  • A variety of chemically modified agaroses with different melting and gelling temperatures are available through chemical modifications. (wikipedia.org)
  • Researchers found that the main principles of Tsvet's chromatography could be applied in many different ways, resulting in the different varieties of chromatography described below. (wikipedia.org)
  • However
  • Synthetic methylation however have the reverse effect, whereby increased methylation lowers the gelling temperature. (wikipedia.org)
  • degradation
  • Hydrolytic degradation can provide for dissolution of the gel components and for controlled release of trapped molecules, including drugs. (google.com)
  • forms
  • Agarose is available as a white powder which dissolves in near-boiling water, and forms a gel when it cools. (wikipedia.org)
  • work
  • Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry. (wikipedia.org)
  • Volume
  • Gel filtration has the advantage of speed (a few minutes vs. hours for dialysis), though, along with the ability to remove contaminants from relatively small-volume samples compared to dialysis, an important feature when working with toxic or radioactive substances. (wikipedia.org)
  • available
  • At Patents you can conduct a Patent Search, File a Patent Application, find a Patent Attorney, or search available technology through our Patent Exchange. (patents.com)
  • water
  • On standing the agarose gels are prone to syneresis (extrusion of water through the gel surface), but the process is slow enough to not interfere with the use of the gel. (wikipedia.org)
  • positive
  • Dendrotoxins: Dendrotoxins inhibit neurotransmissions by blocking the exchange of positive and negative ions across the neuronal membrane lead to no nerve impulse, thereby paralysing the nerves. (wikipedia.org)