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  • proteins
  • eng] The ion-exchange chromatography is a technique used to separate the milk whey proteins ß-Lactoglobulin A and B. These proteins are very important to obtain different products for the food industry. (upv.es)
  • fractionation
  • This is due to the optimization of enrichment protocols for phosphoproteins and phosphopeptides, better fractionation techniques using chromatography, and improvement of methods to selectively visualize phosphorylated residues using mass spectrometry. (wikipedia.org)
  • zwitterionic
  • With that exception, HILIC phases can be grouped into five categories of neutral polar or ionic surfaces: simple unbonded silica silanol or diol bonded phases amino or anionic bonded phases amide bonded phases cationic bonded phases zwitterionic bonded phases A typical mobile phase for HILIC chromatography includes acetonitrile ("MeCN", also designated as "ACN") with a small amount of water. (wikipedia.org)
  • Reversed-Phase
  • This methodology employs isoelectric focusing of peptides on immobilized, pH gradient (IPG) gels in the first dimension, followed by reversed-phase chromatography in the second dimension, and subsequent tandem mass spectrometry analysis. (rti.org)
  • For the detection of polar compounds with the use of electrospray-ionization mass spectrometry as a chromatographic detector, HILIC can offer a ten fold increase in sensitivity over reversed-phase chromatography because the organic solvent is much more volatile. (wikipedia.org)
  • The derivatized amino acids are subjected to reversed phase chromatography, typically using a C8 or C18 silica column and an optimised elution gradient. (wikipedia.org)
  • Higher selectivity: for example, positive, negative and neutral substances could be separated by a reversed phase (RP)/anion-cation exchange (ACE) column in a single run. (wikipedia.org)
  • Column
  • If it is stable at pH values below the pI, the cation exchange column be used. (wikipedia.org)
  • and subjected to ion-exchange chromatography in a DEAE-cellulose column (Sigma Chemical) equilibrated and percolated with 50 mM AcNa buffer until complete removal of non-retained polysaccharides, followed by SP fractioning by elution with the same equilibrium buffer containing NaCl in different concentrations (0. (thefreedictionary.com)
  • However, there are also disadvantages involved when performing ion-exchange chromatography, such as constant evolution with the technique which leads to the inconsistency from column to column. (wikipedia.org)
  • An example of the ion-exchange chromatography is given by the NTRC using sulfonated polystyrene as a matrix, adding the amino acids in acid solution and passing a buffer of steadily increasing pH through the column. (wikipedia.org)
  • applications
  • MSS was established as there were no dedicated conferences to this technology and to bring together the top international scientists and researchers in the area of monolith chromatography to share their experiences and innovative applications. (wikipedia.org)
  • techniques
  • Further, Cellufine MAX ion exchange (IEX) media are made using surface modification techniques that dramatically increase ligand availability, which translates to higher dynamic binding capacities. (amsbio.com)
  • Not until 1975 was "ion chromatography" established as a name in reference to the techniques, and was thereafter used as a name for marketing purposes. (wikipedia.org)
  • Analysis
  • Because of the abundant separating columns, elution systems, and detectors available, chromatography has developed into the main method for ion analysis. (wikipedia.org)
  • media
  • The differences between X-h and X-r type Cellufine MAX strong ion exchange media (X) are due to the design of the media. (amsbio.com)