The first system was manual and involved the use of a fluorescence microscope to measure the absolute nuclear fluorescent intensity (ANFI) of acridine-orange (AO) stained cells. (cdc.gov)
The handheld UV analyzer is widely used for nucleic acid testing (observe fluorescence) in many industries including molecular biology, biological chemistry, medical science, forensic autopsy examination as well as biological agent. (jualalatlab.com)
In the Kawamoto technique, blood smears on a slide are stained with acridine orange and examined with either a fluorescence microscope or a light microscope adapted with an interference filter system. (cdc.gov)
The acridine orange test (AOT) exemplifies the second type of assay and differentiates sperm with normal double-stranded DNA (green fluorescence) and abnormal denatured or single-stranded DNA (orange-red fluorescence) with the help of the metachromatic shift properties of the stain [ 10 , 11 ]. (ecerm.org)
4. Yagci A, Murk W, Stronk J, Huszar G. Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. (dergipark.org.tr)
Ethidium4
Apoptosis was checked by acridine orange/ethidium bromine staining assay and by execution of Western blotting analysis. (ijpsonline.com)
Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
LWA treatment could induce apoptosis in ascites cells evidenced by acridine orange/ethidium bromide, Hoechst, and Giemsa staining. (gp120inhibitor.com)
The Cytotoxicity of methanolic extract was tested by Brine shrimp lethality assay, Trypan blue dye exclusion method, Neutral red Cytotoxicity assay, MTT assay and Ethidium bromide - acridine orange staining. (iajpr.com)
Giemsa1
Various stains like Giemsa and Acridine Orange, are used along with wet mount examination for the microscopic diagnosis of Trichomonas vaginalis . (sigmaaldrich.com)
Microscopic examination4
Microscopic examination of vaginal secretions in saline shows moving organisms. (wdxcyber.com)
For example, a urine microscopic examination might report: 0-5 red blood cells seen per high power field. (who.int)
Microscopic examination of cells collected within the plume also revealed that the dominant cell type exhibits a distinctive morphology typical of the Oceanospirillales . (lbl.gov)
Colors and staining are often utilized in the field of histology (microscopic examination of living tissues) as well as in areas of medical histopathology, Hematology, and Cytopathology which are focused on studying and diagnosis of disease at a microscopic scale. (microbiologynote.com)
Dyes2
Morphological features of the roots were assessed using a root scanner, and then attempts were made to stain the roots in four types of dyes: 0.01% methylene blue, 0.01% acridine orange, 0.01% malachite green, and 0.01% carbol fuchsin. (scirp.org)
Ingenuity pathway examination the dyes regulated genes in pediatric AML To investigate possible biological Inhibitors,Modulators,Libraries interactions of differ ently regulated genes, datasets representing genes with altered expression profile derived from serious time PCR array analyses had been imported in to the Ingenuity Pathway Evaluation Instrument. (micrornainhibitors.com)
Qualitative examinations are those that measure the presence and semi- or absence of a substance, or evaluate cellular characteristics quantitative such as morphology. (who.int)
Examples of qualitative examinations include microscopic examinations for cell morphology or presence of parasitic organisms, serologic procedures for presence or absence of antigens and antibodies, some microbiological procedures, and some molecular techniques. (who.int)
Chromatin1
Signals will be analysed with software programming in examination of both sperm DNA fragmentation (sDF) and atypical chromatin structure. (wikipedia.org)
Light microscope1
Rapid diagnosis of malaria using acridine orange (AO) staining and a light microscope with a halogen lamp and interference filter was deployed in some malaria-endemic countries. (biomedcentral.com)
Fluorescent1
Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. (researchgate.net)
Physical Examination1
No single measurement or criterion reliably predicts fertility, and therefore several criteria are usually evaluated, including breeding ability and libido, general physical examination and inspection of the genital organs, as well as assessment of sperm production and quality. (merckvetmanual.com)
Apoptosis1
HDACs one is vital in en hancing cytarabine induced apoptosis in pediatric AML, not less than partly mediated by Bim. (microrna21.com)
Laboratory1
methods are applied to qualitative and semi-quantitative laboratory examinations. (who.int)
Abnormal1
Furthermore, in a repeated dose oral study equivalent to OECD 408, all rats dosed with 250, 500 or 1000 mg/kg bw/day for 90 days survived, and no abnormal symptoms related to nano calcium carbonate administration were observed through general clinical observation and eye examination. (europa.eu)
Tissues1
During microscopic examinations, a satisfactory contrast was noted between root tissues and the structures of mycorrhizal fungi. (scirp.org)
Cells1
Some microscopic examinations are considered semi- quantitative because results are reported as estimates of the number of cells seen per low power field or high power field. (who.int)
Samples3
These controls may be built-in (on-board, or procedural) controls, traditional controls that mimic patient samples, or may consist of stock cultures for use with microbiological examinations. (who.int)
Becton Dickinson) method, blood samples are collected in a special tube containing acridine orange, an anticoagulant, and a float, and then are centrifuged in a microhematocrit centrifuge. (cdc.gov)
SDF was assessed using the acridine orange test on density gradient centrifugation prepared samples. (ecerm.org)
Blood1
Microscopical examination (ME) of stained blood films remains the gold standard method for the detection of these infections in birds, particularly because co-infections predominate in wildlife. (biomedcentral.com)
Types1
Qualitative and semi-quantitative examinations include tests that utilize a variety of types control materials. (who.int)
Presence1
Examination of some of these workers indicated the presence of a damaged urothelium consistent with premalignant conditions. (cdc.gov)
Significant1
IPA examination showed the best significant pathways for pediatric AML are p53 and Huntingtons illness sig naling. (micrornainhibitors.com)
Primary1
Reagents required for this multi-step staining process include crystal violet (primary stain), aniline dye, iodine solution (mordant), and safranin orange (secondary stain), or carbol fuchsin counterstains. (sigmaaldrich.com)
Sperm DNA fragmentation2
[ 4 ] The recently published 6th edition of the WHO laboratory manual for the examination and processing of human semen has acknowledged such limitations, and in an attempt to identify and underline the importance of sperm function testing, it has introduced sperm DNA fragmentation (SDF) as an extended examination and recommends this assessment in certain clinical settings, which has been quoted as a major strength of the new manual. (medscape.com)
Signals will be analysed with software programming in examination of both sperm DNA fragmentation (sDF) and atypical chromatin structure. (wikipedia.org)
Physical1
Medical students, practitioners, health care institutions, school officials, and local businesses cooperated to provide a program that included physical examinations, educational sessions, entertainment, and information on health careers, especially for young people from "minority groups. (nih.gov)
Measure1
Qualitative examinations are those that measure the presence and semi- or absence of a substance, or evaluate cellular characteristics quantitative such as morphology. (who.int)