• MCB probes are established by chromosome microdissection followed by whole genomic DNA amplification. (biomedcentral.com)
  • Comparison with the recently reported two-superdomain structure of the human inactive X shows that the genomic content of the superdomains differs between species, but part of the boundary region is conserved and located near the Dxz4 / DXZ4 locus. (biomedcentral.com)
  • By applying a novel Hi-C method to map allelic chromatin contacts, we discover a specific bipartite organization of the mouse inactive X chromosome that probably plays an important role in maintenance of gene silencing. (biomedcentral.com)
  • Chromosomes occupy specific territories within the nucleus [ 1 ]. (biomedcentral.com)
  • The heterochromatization of one of the X chromosomes in female somatic cells is initiated by the long non-coding RNA (lncRNA) Xist that coats the Xi in early embryogenesis and silences transcription by recruiting specific proteins that put in place repressive histone modifications such as tri-methylation of histone H3K27, ubiquitination of histone H2AK119, and de-acetylation [ 9 - 12 ]. (biomedcentral.com)
  • The workflow was successfully applied for murine chromosomes that are difficult to identify unambiguously. (biomedcentral.com)
  • The absolutely necessary condition for chromosome microdissection is the possibility to identify the target chromosome unambiguously. (biomedcentral.com)
  • The inactive X chromosome (Xi) becomes highly condensed compared with the active X (Xa) and forms the Barr body, often visible as a dense region within the nucleus [ 8 ]. (biomedcentral.com)