Nuclear Localization SignalsCell NucleusActive Transport, Cell Nucleusalpha KaryopherinsMolecular Sequence DataAmino Acid SequenceKaryopherinsbeta KaryopherinsCytoplasmProtein Sorting SignalsNuclear ProteinsNuclear Export SignalsRecombinant Fusion ProteinsHeLa CellsProtein TransportGreen Fluorescent ProteinsProtein Structure, TertiaryBase SequenceCell LineCOS CellsSequence Homology, Amino AcidLuminescent ProteinsMutationran GTP-Binding ProteinProtein BindingDNA-Binding ProteinsSequence DeletionTransfectionTranscription FactorsSignal TransductionSubcellular FractionsCell NucleolusConserved SequenceNucleoplasminsCell CompartmentationCercopithecus aethiopsAmino Acid MotifsMicroscopy, FluorescenceBiological TransportCloning, MolecularBinding SitesMutagenesis, Site-DirectedSequence AlignmentRNA-Binding ProteinsNuclear EnvelopeMicroscopy, ConfocalDNA, ComplementaryPhosphorylationZinc FingersFluorescent Antibody TechniquePlasmidsAmino Acids, BasicSaccharomyces cerevisiae ProteinsRecombinant ProteinsCarrier ProteinsSaccharomyces cerevisiaeRNA, MessengerTwo-Hybrid System TechniquesTranscription, GeneticDNA PrimersViral ProteinsBlotting, WesternFluorescent Antibody Technique, Indirectbeta-GalactosidaseNucleocytoplasmic Transport ProteinsReceptors, Cytoplasmic and NuclearCells, CulturedProtein IsoformsMutagenesis3T3 CellsGenes, ReporterDrosophila ProteinsNuclear PoreEvolution, MolecularPhosphoproteinsTrans-ActivatorsModels, BiologicalTranscriptional ActivationRepressor ProteinsGene Expression RegulationNIH 3T3 CellsAntigens, Polyomavirus TransformingCell Cycle ProteinsFungal ProteinsImmunohistochemistryDNAAlternative SplicingFatty Acids, UnsaturatedN-Acylneuraminate CytidylyltransferaseNuclear Pore Complex ProteinsMembrane ProteinsPlant ProteinsImmunoprecipitationProteinsDimerizationAT-Hook MotifsDrosophila melanogasterNuclear MatrixProtein-Serine-Threonine KinasesPoint Mutation