• The substituted amino acid is located within the second C2HC motif in the conserved zinc finger domain of NANOS3 and in silico molecular modelling suggests destabilization of protein-RNA interaction. (hindawi.com)
  • The first phosphorylation site is located in a turn motif, the second one at the end of the domain in an hydrophobic pocket. (embl.de)
  • and a magnesium binding site: Walker B. Besides these two regions, three other conserved motifs are present in the ABC cassette: the switch region which contains a histidine loop, postulated to polarize the attaching water molecule for hydrolysis, the signature conserved motif (LSGGQ) specific to the ABC transporter, and the Q-motif (between Walker A and the signature), which interacts with the γ phosphate through a water bond. (expasy.org)
  • The adenovirus E1A protein mimics the LxDLFD motif and competes with E2F for binding to Rb, promoting E2F activation and cell proliferation. (eu.org)
  • The LxCxE motif binds to all pocket proteins and is present in chromatin regulators such as HDAC and KDM5A and in viral proteins. (eu.org)
  • The LxDLFD motif ( LIG_RB_pABgroove_1 ) mediates binding to a highly conserved deep groove formed in the interface between the A and B subdomains of the Rb, p107 and p130 pocket domains. (eu.org)
  • A five-residue motif for the design of domain swapping in proteins. (ncbs.res.in)
  • Finally, we contrast the regulatory properties of protein motifs and the regulatory elements of DNA and (pre-)mRNAs, advocating that co-regulation, co-operativity, and motif-driven regulatory programs are common mechanisms that emerge from the use of simple, evolutionarily plastic regulatory modules. (biomedcentral.com)
  • This modification alters the properties of the nucleosome and affects its interactions with other proteins, particularly in regards to gene transcription processes. (wikipedia.org)
  • Thus, it is conceivable that (P)RR utilizes its "hand" to catch two binding partners in a promiscuous manner and tether them closely in space to facilitate protein-protein interactions . (globalwomenshealthacademy.org)
  • Throughout evolution, eukaryotes adopted a network of highly conserved proteins that buffer the positive charges of histones, maintaining their solubility and, therefore, avoiding aberrant interactions with other cellular components. (aging-us.com)
  • We found that interactions among pentamers in the HPeV-1 capsid are mediated by the N termini of VP0s, which correspond to the capsid protein VP4 and the N-terminal part of the capsid protein VP2 of other picornaviruses. (omicsdi.org)
  • Therefore, putative small molecules that could disrupt HPeV RNA-capsid protein interactions could be developed into antiviral inhibitors.Human parechoviruses (HPeVs) are pathogens that cause diseases ranging from respiratory and gastrointestinal disorders to encephalitis. (omicsdi.org)
  • Targeting YAP/TAZ-TEAD protein-protein interactions using fragment-based and computational modeling approaches. (omicsdi.org)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • The corresponding position in E2F1/2 (D428/429) faces the solvent and does not form interactions, suggesting one additional residue in helical conformation, as observed in E1A, is required for proper positioning of the last acidic residue. (eu.org)
  • A structure of the phosphomimetic S608E mutant ( 4ELL ) reveals a conserved helical structure, conserved positioning of hydrophobic residues and conserved interactions for the two acidic positions (D604 and E608) ( Burke,2012 ). (eu.org)
  • Destabilization of polar interactions in the prion protein triggers misfolding and oligomerization. (ncbs.res.in)
  • 2018. Mechanism of aggregation and membrane interactions of mammalian prion protein. . (ncbs.res.in)
  • 2017. A Dry Transition State More Compact Than the Native State Is Stabilized by Non-Native Interactions during the Unfolding of a Small Protein. . (ncbs.res.in)
  • A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
  • We reveal the potential to exploit interactions of sPLA 2 s with other proteins in medical terms, for the development of original diagnostic and therapeutic procedures. (ijbs.com)
  • Previous reports have demonstrated that the receptor forms a dimer, meaning that the two protein units are bound together to form one assembly unit. (globalwomenshealthacademy.org)
  • Chemokines are assigned to three different families based on the number and position of conserved cysteine residues (Van Coillie et al. (justia.com)
  • The α and β chemokines each contain four conserved cysteine residues. (justia.com)
  • By contrast, lymphotactin is the sole member of the third family of chemokines, and contains only the second and fourth conserved cysteine residues. (justia.com)
  • This mutant shows altered subcellular localization and reduced S-palmitoylation, a phenotype copied by mutation of conserved cysteine residues in microbat IFITM3. (life-science-alliance.org)
  • Furthermore, we show that microbat IFITM3 is S-palmitoylated on cysteine residues C71, C72, and C105, mutation of each cysteine individually impairs virus restriction, and a triple C71A-C72A-C105A mutant loses all restriction activity, concomitant with subcellular re-localization of microbat IFITM3 to Golgi-associated sites. (life-science-alliance.org)
  • The interface between MTERF4 and NSUN4 contains evolutionarily conserved polar and hydrophobic amino acids, and mutations that change these residues completely disrupt complex formation. (rcsb.org)
  • Histone methylation is a process by which methyl groups are transferred to amino acids of histone proteins that make up nucleosomes, which the DNA double helix wraps around to form chromosomes. (wikipedia.org)
  • Proteins are chains of amino acids. (globalwomenshealthacademy.org)
  • ABC transporters are minimally constituted of two conserved regions: a highly conserved ATP binding cassette (ABC) and a less conserved transmembrane domain (TMD). (expasy.org)
  • DNA substitution matrices DNA is less conserved than protein sequences. (progsound.de)
  • External application of synthetic peptides increases the abundance of the targeted protein, leading to related phenotypes. (bvsalud.org)
  • Here, we identify an evolutionarily conserved hexapeptide sequence as the major aggregation-prone region (APR) of gastrointestinal peptides of the glucagon family: xFxxWL. (nature.com)
  • The peptide exit tunnel is lined with hydrophobic residues, which stabilise the highly hydrophobic nascent mitochondrial peptides 4 . (nature.com)
  • It was discovered that the gene, called an early nodulin 40 (Enod40), previously annotated as transcribed with the formation of lncRNA encodes actually two short peptides (with a length of 12 and 24 amino acid residues) in plants, where they participate in the organogenesis of the root nodules [10, 11]. (fortunepublish.com)
  • It turned out that four peptides, encoded by a number of long non-coding RNAs, have a length of 11 to 32 residues and are necessary for embryonic development of flies [13, 14]. (fortunepublish.com)
  • Antimicrobial proteins and peptides offer a multifaceted mechanism suitable to fight bacterial resistance. (frontiersin.org)
  • The juxtamembrane domain has two highly conserved tyrosine that regulate activation of catalytic core of the receptor. (genominfo.org)
  • sPLA 2 s contain 6 to 8 disulphide bonds, a highly conserved His/Asp catalytic dyad, and a Ca 2+ -binding loop [ 1 ]. (ijbs.com)
  • This study provides a molecular explanation for MTERF4-dependent recruitment of NSUN4 to ribosomal RNA and suggests a unique mechanism by which other members of the large MTERF-family of proteins can regulate ribosomal biogenesis. (rcsb.org)
  • Studies of these sites have found that methylation of histone tails at different residues serve as markers for the recruitment of various proteins or protein complexes that serve to regulate chromatin activation or inactivation. (wikipedia.org)
  • AGC kinase proteins are characterised by three conserved phosphorylation sites that critically regulate their function. (embl.de)
  • Allosteric communication in proteins can be induced by the binding of effective ligands, mutations or covalent modifications that regulate a site distant from the perturbed region. (biomedcentral.com)
  • Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
  • They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
  • The LxCxE and LxSxE motifs bind to a highly conserved cleft in the B subdomain. (eu.org)
  • We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
  • Studies of IFITM topology suggest a type II transmembrane configuration with a cytosolic N terminus, cytosolic conserved intracellular loop (CIL) domain, transmembrane domain, and extracellular (or intraluminal) C terminus ( 4 , 5 ), although there is evidence that other IFITM topologies exist ( 6 , 7 , 8 ). (life-science-alliance.org)
  • IFITMs belong to the CD225/pfam04505 or "dispanin" protein superfamily ( http://pfam.xfam.org/family/PF04505 ) ( 3 ) that contains more than 2,000 members, including both prokaryotic and eukaryotic proteins, all of which encode a conserved CD225 protein domain. (life-science-alliance.org)
  • The fundamental unit of chromatin, called a nucleosome, contains DNA wound around a protein octamer. (wikipedia.org)
  • Along with PTMs, the partial or complete disassembly of nucleosomes allows the exchange and degradation of pre-existing histone proteins, with the incorporation of newly synthesised histones onto chromatin that can eventually result in the resetting of previous epigenetic marks. (aging-us.com)
  • These dedicated proteins are generally known as histone chaperones and effectively control histone supply and chromatin dynamics [ 8 ]. (aging-us.com)
  • AMA-1 and RON2 of P. gallinaceum are evolutionarily and structurally conserved, suggesting that these proteins may play essential roles for avian malaria parasites to invade host erythrocytes. (biomedcentral.com)
  • Ephs belong to the largest family of receptor tyrosine kinase and are highly conserved both sequentially and structurally. (genominfo.org)
  • Functionally essential amino acid residues are evolutionarily conserved and clustered to form functional patches on the protein surface. (globalwomenshealthacademy.org)
  • Quantified proteins were classified into 23 functional categories and their abundance compared between sexes. (biomedcentral.com)
  • Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins. (embl.de)
  • Proteins known to belong to this family are classified in several functional subfamilies depending on the substrate used [ E1 ]. (expasy.org)
  • All different types of transporters with a functional attribution are listed below (references are only provided for recently characterized proteins). (expasy.org)
  • Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)
  • Pocket proteins play essential roles in cell cycle progression, quiescence and differentiation, and their functional disruption is associated with human cancer. (eu.org)
  • Mitochondrial porin , also known as the voltage-dependent anion channel (VDAC), is a multi-functional channel protein that shuttles metabolites between the mitochondria and the cytosol and implicated in cellular life and death decisions. (sdbonline.org)
  • The RNA-binding protein NANOS3 poses as an interesting candidate gene for POI since members of the Nanos family have an evolutionarily conserved function in germ cell development and maintenance by repressing apoptosis. (hindawi.com)
  • The identification of an inactivating missense mutation in NANOS3 suggests a mechanism for POI involving increased primordial germ cells (PGCs) apoptosis during embryonic cell migration and highlights the importance of NANOS proteins in human ovarian biology. (hindawi.com)
  • Nanos was first identified in Drosophila , where it represses the translation of target mRNAs through binding to their 3′ UTR and has a conserved function in germ cell development across species. (hindawi.com)
  • Members of the evolutionarily conserved Nanos gene family are preferentially expressed in the ovaries and are known to play an important role in germ cell development, maintenance, and survival [ 24 - 30 ]. (hindawi.com)
  • The Drosophila Nanos protein is a localized repressor of hunchback mRNA translation in the early embryo, and is required for the establishment of the anterior-posterior body axis. (embl.de)
  • Analysis of nanos mutants reveals that a small, evolutionarily conserved, C-terminal region is essential for Nanos function in vivo, while no other single portion of the Nanos protein is absolutely required. (embl.de)
  • We show that full-length and C-terminal Nanos proteins bind to RNA in vitro with high affinity, but with little sequence specificity. (embl.de)
  • m6A mostly mediates its effect by recruiting reader proteins that either directly accommodate the modified residue in a hydrophobic pocket formed by their YTH domain, or otherwise have their affinity positively influenced by the presence of m6A. (bvsalud.org)
  • VCP primarily identifies ubiquitylated proteins in these pathways and mediates their unfolding and degradation by the 26S proteasome. (portlandpress.com)
  • Therefore, considering the importance of Eph-ephrin class of protein in tumorigenesis, 7,620 clinically reported missense mutations belonging to the class of variables of unknown significance were retrieved from cBioPortal and evaluated for pathogenicity. (genominfo.org)
  • After translation, where is a protein localised, and what complexes should it join? (biomedcentral.com)
  • Experimental binding data (ΔΔG values) of alanine-mutated interface residues of known protein complexes have been mostly widespread for estimating the residue-contribution to the binding event (i.e. finding the binding hot spots). (biomedcentral.com)
  • Protein crowding induces membrane curvatures through an entropic mechanism. (portlandpress.com)
  • The prevalence and similarities of these signaling filopodia suggest that the polarized target-specific morphogen exchange through filopodial contacts is an evolutionarily conserved signaling mechanism. (biorxiv.org)
  • Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
  • 2023. Replacement of the native cis prolines by alanine leads to simplification of the complex folding mechanism of a small globular protein. . (ncbs.res.in)
  • 2018. Site-specific time-resolved FRET reveals local variations in the unfolding mechanism in an apparently two-state protein unfolding transition. . (ncbs.res.in)
  • These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis and shed light on a link between Rbf1 and mitochondrial dynamics, in vivo. (sdbonline.org)
  • We recently showed that a member of the mitochondrial transcription termination factor (MTERF) family of proteins, MTERF4, recruits NSUN4, a 5-methylcytosine RNA methyltransferase, to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. (rcsb.org)
  • We uncovered the functions of ABCE proteins Rli1/ABCE1 and Arb1 in PIC assembly and ribosome biogenesis, and identified the tRNA methyltransferase Gcd10/Gcd14, which contributed to the discovery of the TRAMP-mediated RNA surveillance pathway. (nih.gov)
  • Together, our results suggest that the EMC engages with DENV polyproteins to ensure proper biogenesis of the NS4A-NS4B region, and provide further evidence for the cellular function of the EMC in the stable expression of TM proteins. (biorxiv.org)
  • The first two conserved cysteines of the β chemokines are adjacent. (justia.com)
  • Co-transport of gN and gM to the trans Golgi network also occurs upon replacement of conserved cysteines in gM and gN, suggesting that their physical interaction is mediated by covalent and non-covalent forces. (mdpi.com)
  • This complex process is evolutionary conserved and involves several RNA processing and modification steps required for correct ribosomal RNA maturation. (rcsb.org)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Lipid transport proteins at membrane contacts, where organelles are closely apposed, are critical in redistributing lipids from the endoplasmic reticulum (ER), where they are made, to other cellular membranes. (rupress.org)
  • We identify mitoguardin-2, a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), as a lipid transporter. (rupress.org)
  • In dry pollen, LARP6C binds to transcripts encoding proteins that function in lipid synthesis and homeostasis, vesicular trafficking, and polarized cell growth. (bvsalud.org)
  • Higher cells of download Hanging Sam: A Military and mental Canadian initiation in the 5'-end novo depends the lipid protein directly than NALP1-mediated deafness to the chain. (evakoch.com)
  • 2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)
  • Similar to cellular TM proteins, the viral polyprotein relies on the host cell machinery for targeting to the ER, translocation across the membrane and insertion of transmembrane domains (TMDs) into the lipid bilayer during its translation. (biorxiv.org)
  • These 18 residues CCHC zinc finger domains are mainly found in the nucleocapsid protein of retroviruses. (embl.de)
  • While RNA makes up most of the composition of bacterial and cytosolic eukaryotic ribosomes, mammalian mitochondrial ribosomes present a more elaborate protein shell, which aids coping with the oxidative microenvironment. (nature.com)
  • Almost half of these MRPs are evolutionarily exclusive to mitochondrial ribosomes, some of which were repurposed and accreted during reductive genome evolution 4 , 5 . (nature.com)
  • The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
  • Each addition of a methyl group on each residue requires a specific set of protein enzymes with various substrates and cofactors. (wikipedia.org)
  • Heme/iron-binding proteins, protease inhibitors, proteases, lipocalins and immune-related proteins were the categories most abundantly expressed in females, while glycolytic enzymes, protease inhibitors and lipocalins were the most abundantly expressed in males. (biomedcentral.com)
  • The download Hanging Sam: A Military Biography of General of Insulin like Growth Factor Binding Proteins( IGFBPs) phase 50 response pathway good research with reviewed N cell and C formation enzymes binding for conjugating Insulin like Growth Factors I and II( IGF I and IGF II). (evakoch.com)
  • The mitochondria have their own chaperones and proteolytic enzymes that remove damaged or unfolded proteins [ 18 - 20 ]. (hindawi.com)
  • An x-ray structure shows that the C-terminal domain of mitoguardin-2 has a hydrophobic cavity that binds lipids. (rupress.org)
  • In general, most genes that encode antigens of Plasmodium parasites are highly polymorphic and encode proteins that are important targets for host protective antibody responses [ 5 ]. (biomedcentral.com)
  • Previously, non-coding RNAs (ncRNAs), including micro-RNAs (miRNAs) and other long non-coding RNAs (lncRNAs), have been generally considered unable to encode proteins both in plants and animals [1-9]. (fortunepublish.com)
  • Methylation events that weaken chemical attractions between histone tails and DNA increase transcription because they enable the DNA to uncoil from nucleosomes so that transcription factor proteins and RNA polymerase can access the DNA. (wikipedia.org)
  • N6-methyl adenosine (m6A) is the most prevalent and evolutionarily conserved, modification of polymerase II transcribed RNAs. (bvsalud.org)
  • In collaboration with Jon Lorsch's group, we demonstrated that segments/residues in eIF1, eIF1A, tRNAi and 18S rRNA, which are implicated genetically in TC recruitment, also stimulate the rate of this reaction in a fully reconstituted yeast in vitro translation system. (nih.gov)
  • Valosin-containing protein (VCP, also p97, or Cdc48p in yeast) is an evolutionarily conserved, homo-hexameric, ubiquitin-selective, AAA-ATPase that functions in numerous ubiquitin-dependent protein quality control pathways. (portlandpress.com)
  • Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)
  • Overall, (P)RR functions as a scaffold protein, a hub for controlling the spatial and temporal organization of molecules within a cell, and thereby the flow of cellular information. (globalwomenshealthacademy.org)
  • The AAA-ATPase (ATPases associated with diverse cellular activities) valosin-containing protein (VCP), is essential for many cellular pathways including but not limited to endoplasmic reticulum-associated degradation (ERAD), DNA damage responses, and cell cycle regulation. (portlandpress.com)
  • Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. (embl.de)
  • Furthermore, the kinase and SAM domains allow the binding of other cellular proteins. (genominfo.org)
  • The EMC facilitates accurate insertion, topology and/or stabilization of specific cellular TM proteins including a subset of tail-anchored proteins and G protein-coupled receptors. (biorxiv.org)
  • Cells express a pool of thousands of different proteins that need to be tightly controlled for proper cellular structure, organization, and function. (hindawi.com)
  • This finding suggests that both subunits of the protein complex likely contribute to RNA recognition. (rcsb.org)
  • 2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
  • Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)
  • Researchers have visualized the residue positions and evolutionary sequence conservation profiles simultaneously on the 3D structure of (P)RR. (globalwomenshealthacademy.org)
  • In PAM, unlike in BLOSUM, the higher numbers correspond to greater evolutionary distances between proteins. (progsound.de)
  • Suppose I start with a given polypeptide sequence M at time t, and observe the evolutionary changes in the sequence until 1 of all amino acid residues have undergone substitutions at time tn. (progsound.de)
  • In 2021, significant progress in protein 3D structure prediction was made using AlphaFold2 and RoseTTAFold, in which a protein 3D structural model was generated using machine learning algorithms with amino acid sequences as the only input. (globalwomenshealthacademy.org)
  • What is the difference between them and which version of them should I use How do I know which substitution matrix to use in my algorithm when trying to align protein sequences. (progsound.de)
  • Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
  • 2021. Mapping Distinct Sequences of Structure Formation Differentiating Multiple Folding Pathways of a Small Protein. . (ncbs.res.in)
  • VCP identifies ubiquitylated substrates through numerous dedicated adaptor proteins and unfolds substrates by threading them through a central pore in the hexamer ( Figure 1 ). (portlandpress.com)
  • VCP interacts with adaptor proteins to identify ubiquitylated substrates for degradation by the proteasome. (portlandpress.com)
  • Here we define the precise Rab29 binding region of the LRRK2 Armadillo domain between residues 360-450 and show that this domain, termed 'Site #1', can also bind additional LRRK2 substrates, Rab8A and Rab10. (stanford.edu)
  • much 75 cytosol of pairing substrates are in 1500 220 KDa proteins with IGFBP3 and ALS. (evakoch.com)
  • The structural diversity of sPLA 2 -BPs reveals sPLA 2 s as very promiscuous proteins, and we offer some structural explanations for this nature that makes these proteins evolutionarily highly advantageous. (ijbs.com)
  • Each protein adopts a unique 3D shape based on its amino acid sequence (the order of amino acid residues). (globalwomenshealthacademy.org)
  • The AGC (cAMP-dependent, cGMP-dependent and protein kinase C) protein kinase family embraces a collection of protein kinases that display a high degree of sequence similarity within their respective kinase domains. (embl.de)
  • BLOSUM62 is the matrix calculated by using the observed substitutions between proteins which have at most 62 sequence identity, etc. replacements are counted on the branches of a phylogenetic. (progsound.de)
  • These processes are facilitated by the signal recognition particle (SRP), the translocon-associated protein (TRAP) complex (also known as signal-sequence receptor complex), the Sec61 translocon and the signal peptidase complex. (biorxiv.org)
  • For most TM proteins, recognition of a hydrophobic TMD or signal sequence by the SRP followed by transfer to the Sec61 translocation channel are sufficient for membrane targeting and accurate topogenesis ( Shao and Hegde, 2011 ). (biorxiv.org)
  • 2022. Differentiating between the sequence of structural events on alternative pathways of folding of a heterodimeric protein. . (ncbs.res.in)
  • Such protein-mediated transfer is especially important for maintaining organelles disconnected from secretory pathways, like mitochondria. (rupress.org)
  • Most resulting proteins lead in techniques with IGFBPs, which plan methylated to modulate the CLASP of acts in the mode, react salt of IGFs to trim members for mice, promote cornea like supplements of IGFs, and be as encoding mitochondria here of IGFs. (evakoch.com)
  • 2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
  • 2021. Microsecond dynamics during the binding-induced folding of an intrinsically disordered protein. . (ncbs.res.in)
  • Prokaryotic importers require additional extracytoplasmic binding proteins (one or more per systems) for function. (expasy.org)
  • The two regions mapped onto the structural model formed a continuous surface patch comprising evolutionarily conserved hydrophobic residues. (globalwomenshealthacademy.org)
  • 2015 . The crystal structure of full-length E. coli YidC revealed that a hydrophilic groove, formed by five transmembrane helices, is a conserved structural feature of YidC, as compared to the previous YidC structure from Bacillus halodurans , which lacks a periplasmic domain. (tcdb.org)
  • 2021. Structural Characterization of the Cooperativity of Unfolding of a Heterodimeric Protein Using Hydrogen Exchange-Mass Spectrometry. . (ncbs.res.in)
  • 2017. Identification and structural characterization of the precursor conformation of the prion protein which directly initiates misfolding and oligomerization. . (ncbs.res.in)
  • 2017. Modulation of the Extent of Cooperative Structural Change During Protein Folding by Chemical Denaturant. . (ncbs.res.in)
  • Lastly, we showed a physical interaction between the EMC and DENV NS4B protein post-cleavage and rapid degradation of processed NS4B in the absence of EMC. (biorxiv.org)
  • The proteostasis network (PN) is an assembly of distinct dynamic molecular pathways that control the functionality of the proteome (proteome homeodynamics) during protein synthesis, folding, trafficking, and degradation. (hindawi.com)
  • To facilitate screens for small molecule compounds that disrupt the YAP-TEAD interaction, we have developed the first ultra-bright NanoLuc biosensor to quantify YAP/TAZ-TEAD protein-protein interaction (PPI) both in living cells and also in vitro using biosensor fusion proteins purified from bacteria. (omicsdi.org)
  • The invention provides antibodies that bind to a plurality of β-chemokines, particularly monocyte chemotactic proteins MCP-1, MCP-2 and MCP-3. (justia.com)
  • The ABC module (approximately two hundred amino acid residues) is known to bind and hydrolyze ATP, thereby coupling transport to ATP hydrolysis in a large number of biological processes. (expasy.org)
  • Examples include methylation of lysine 4 of histone 3 (H3K4me1), and arginine (R) residues on H3 and H4. (wikipedia.org)
  • This octamer consists of two copies each of four histone proteins: H2A, H2B, H3, and H4. (wikipedia.org)
  • Histones can be methylated on lysine (K) and arginine (R) residues only, but methylation is most commonly observed on lysine residues of histone tails H3 and H4. (wikipedia.org)
  • Generally, methylation of an arginine residue requires a complex including protein arginine methyltransferase (PRMT) while lysine requires a specific histone methyltransferase (HMT), usually containing an evolutionarily conserved SET domain. (wikipedia.org)
  • Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). (cipsm.de)
  • The identification of protein-protein interaction disruptors (PPIDs) that disrupt the YAP/TAZ-TEAD interaction has gained considerable momentum. (omicsdi.org)
  • Here, a genome-wide analysis of the human mitochondrial transcriptome shows that 2'- O -methylation is limited to residues of the mitoribosomal large subunit (mtLSU) 16S mt-rRNA, introduced by MRM1, MRM2 and MRM3, with the modifications installed by the latter two proteins being interdependent. (nature.com)
  • The residues involved in RNA binding are conserved among all parechoviruses, suggesting a putative role of the genome in virion stability or assembly. (omicsdi.org)
  • Flaviviruses such as dengue (DENV), Zika (ZIKV) or West Nile virus (WNV) translate their genome as a single multi-pass transmembrane (TM) protein at the endoplasmic reticulum (ER) membrane. (biorxiv.org)
  • Here, we show that bat IFITMs are characterized by strong adaptive evolution and identify a highly variable and functionally important site-codon 70-within the conserved CD225 domain of IFITMs. (life-science-alliance.org)
  • Within the RNaseA superfamily there is a group of highly cationic proteins secreted by innate immune cells with anti-infective and immune-regulatory properties. (frontiersin.org)
  • In the second part, we present non YTH-proteins whose roles as m6A readers largely remain to be explored. (bvsalud.org)
  • Pocket proteins Rb, p107 and p130 play central roles in cell cycle progression and differentiation. (eu.org)
  • We hypothesized that the residues with high EVT values play important roles in allosteric signaling. (biomedcentral.com)
  • We also found that the hot spots in protein binding interfaces have significantly high EVT values, which suggests that they play roles in mediating signal communication between protein domains. (biomedcentral.com)
  • We hypothesized that the residues with high EVT values play important roles in allosteric signalling, and found experimental evidences supporting the idea. (biomedcentral.com)
  • Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)
  • There are 212035 ZnF_C2HC domains in 145233 proteins in SMART's nrdb database. (embl.de)
  • Taxonomic distribution of proteins containing ZnF_C2HC domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with ZnF_C2HC domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing ZnF_C2HC domain in the selected taxonomic class. (embl.de)
  • Methylation of some lysine (K) and arginine (R) residues of histones results in transcriptional activation. (wikipedia.org)
  • In the last decades, HOX proteins have been extensively studied due to their pivotal role in transcriptional events. (mdpi.com)
  • Protein-Protein Interaction Disruptors of the YAP/TAZ-TEAD Transcriptional Complex. (omicsdi.org)
  • Their function is decoded by readers that share the YTH domain, which forms a hydrophobic pocket that directly accommodates the m6A residues. (bvsalud.org)
  • For each domain of life, canonical mechanisms and signals are observed to initiate protein synthesis. (cnrs.fr)
  • The two other phosphorylation sites are located outside the kinase domain in a conserved region on its C-terminal side, the AGC-kinase C-terminal domain. (embl.de)
  • Taxonomic distribution of proteins containing S_TK_X domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with S_TK_X domain is also avaliable . (embl.de)
  • These proteins contain two related domains known as the chromo- and the chromoshadow-domain. (cipsm.de)
  • The conserved acidic residue between the first and second hydrophobic residues makes extensive charge contacts with the pocket domain, with D424/425 in E2F1/2 and E45 in E1A forming a salt bridge with R467 in Rb. (eu.org)
  • At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)
  • Mutation of this residue in microbat IFITM3 impairs restriction of representatives of four different virus families that enter cells via endosomes. (life-science-alliance.org)
  • 2017. The G126V Mutation in the Mouse Prion Protein Hinders Nucleation-Dependent Fibril Formation by Slowing Initial Fibril Growth and by Increasing the Critical Concentration. . (ncbs.res.in)
  • Both programs can predict protein structures with near-experimental accuracy. (globalwomenshealthacademy.org)
  • We applied our model to two protein structures as examples, and verified the validity of our model using various types of experimental data. (biomedcentral.com)
  • Historically, allosteric regulation has typically been defined in quaternary structures (e.g., hemoglobin), but now it is recognized as an intrinsic property of all proteins, including monomeric structures [ 8 ]. (biomedcentral.com)
  • Interferon-induced transmembrane proteins (IFITMs) are antiviral factors that act uniquely and early in viral replication cycles to restrict the entry of a diverse range of primarily enveloped viruses into cells ( 1 ). (life-science-alliance.org)
  • Using replicon and immunoblotting studies in EMC KO cells, we demonstrated that the EMC was essential for viral protein expression. (biorxiv.org)
  • Instead, absence of EMC led to a large fraction of expressed viral proteins being targeted to the proteasome post-translationally. (biorxiv.org)
  • These conserved families of secreted morphogens/signals, such as Fibroblast Growth Factor (FGF), Hedgehog (Hh), Wingless (Wg)/Wnt, Epidermal Growth Factor (EGF), and Decapentaplegic (Dpp - a BMP homolog), act away from their sources and, upon binding to receptors, activate gene regulatory pathways to induce functions in recipient cells 1 , 2 . (biorxiv.org)
  • The herpes simplex virus type 1 (HSV-1) glycoprotein N (gN/UL49.5) is a type I transmembrane protein conserved throughout the herpesvirus family. (mdpi.com)
  • Our analysis showed that predicted miPEP-156a micropeptide is evolutionarily conserved in plant family Brassicaceae. (fortunepublish.com)
  • It has been previously shown that rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. (sdbonline.org)
  • Buffy often acts opposite to Debcl , the other Drosophila Bcl-2-family protein. (sdbonline.org)
  • solid course="kwd-title" Keywords: bispecific T-cell engager (BiTE), chimeric antigen receptors (Vehicles), gene-modified adoptive T-cell transfer, Atractylodin T-cell tumor therapy, tumor-specific epitope Intro Tumor cells communicate different epitopes and proteins on the surface area that differentiate them from healthful cells, either by degrees of manifestation or by uncovering novel epitopes not really seen in regular self. (techieindex.net)
  • Despite the well-known fact that primiRNAs are acting as non-protein-coding RNAs, the published data suggest that, in the plant genomes, some pri-miRNAs can also be found in polysomes, and the expression of these miRNA precursors may results in formation of micropeptides which may be involved in regulation of gene expression. (fortunepublish.com)
  • P)RR, in particular, is thought to catch and tether two proteins involved in Wnt/β-catenin signaling, a cell proliferation pathway linked to the pancreatic cancer development. (globalwomenshealthacademy.org)
  • The chloroplast albino 3 (ALB3) protein appears to integrate the light harvesting chlorophyll-binding protein into thylakoid membranes using a pathway that is distinct from the chloroplast Sec translocation pathway. (tcdb.org)
  • Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. (embl.de)
  • Dual specificity protein kinases (e.g. (embl.de)
  • These observations reveal a conserved role for Eph kinases as determinants of topographic map formation in vertebrates and invertebrates (Dearborn, 2002). (sdbonline.org)
  • Subsequently, we used the Gcd− selection to identify domains/residues in eIF2 and tRNAi, eIF1, eIF1A, eIF3, and residues of 18S rRNA located near the 'P' decoding site of the 40S subunit, that participate in rapid TC recruitment in vivo (Figure 1A). (nih.gov)
  • We also study the NPC1 protein that is essential for cholesterol transport in humans and can lead to Niemann Pick C disease when mutated. (stanford.edu)
  • The long term goal of our research is to elucidate the molecular mechanisms by which proteins are targeted to specific and distinct compartments. (stanford.edu)
  • Orthologues identified by transcriptome sequencing were characterized using phylogenetic, ab initio protein modelling and comparative and population-based methods. (biomedcentral.com)
  • Several genetic knockout (KO) screens identified the ER membrane protein complex (EMC) as a critical host component for flavivirus infection. (biorxiv.org)