- Animals, Xenopus laevis: oocytes. (silverchair.com)
- The present study investigated the effect of arachidonic acid on the α- amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, presumably heteromeric receptors formed of GluR1, GluR2, and GluR3, expressed in Xenopus oocytes. (msu.ru)
- Liberation of Ca(2+) from the endoplasmic reticulum (ER) through inositol trisphosphate receptors (IP(3)R) is modulated by the ER Ca(2+) content, and overexpression of SERCA2b to accelerate Ca(2+) sequestration into the ER has been shown to potentiate the frequency and amplitude of IP(3)-evoked Ca(2+) waves in Xenopus oocytes. (ox.ac.uk)
- GABA(A) receptors (alpha1beta2gamma2L) were expressed in both Xenopus oocytes and human embryonic kidney cells exposed to 365 nm light-activated azi-etomidate with or without GABA, then extensively washed. (asahq.org)
- However, this hypothesis began to be questioned when it was found that (i) acrosome-reacted sperm cells are still able to pass through extracellular layers and fertilize the oocyte (ii) most of the sperm cells undergo premature acrosomal reaction before they reach the oocyte. (nusl.cz)
- The oocyte is surrounded by cumulus cells (also termed the corona radiata) from the follicle and spermatozoa must break through this outer layer to reach the oocyte itself (Figure 10.3). (pediagenosis.com)
- Previously, intra- and interspecific canine embryos have been constructed by canine SCNT into canine and bovine oocytes, respectively, but this did not result in viable offspring 9 . (nature.com)
- The activated oocytes were then transferred into the oviducts or uterine horns of recipient dogs at times appropriate to the embryos' developmental stages. (nature.com)
- We collected an average of 12 oocytes from each female, and a total of 1,095 reconstructed canine embryos were transferred into 123 recipients. (nature.com)
- However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. (life-science-alliance.org)
- We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. (life-science-alliance.org)
- Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. (life-science-alliance.org)
- Higher rates of single chromatid abnormalities in oocytes (4), as well as aneuploidy in preimplantation embryos (5) and ongoing pregnancies, are observed in older women. (ijfs.ir)
- Conceive Plus® is compatible with sperm, oocytes, and embryos and can be used by couples trying to conceive. (conceiveplus.eu)
- We collected oocytes matured in vivo at metaphase II about 72 hours after ovulation by flushing the oviducts. (nature.com)
- Subsequently, the glycogen becomes restricted primarily to the inner cumulus, and the corona cells began to retreat from the zona pellucida surface to form an unusual very distinct perizonal space that is clearly evident at the time of ovulation. (elsevierpure.com)
- These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
- With ovulation the secondary oocyte (or ovum) is expelled from the follicle on the ovary surface. (pediagenosis.com)
- The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. (biomedcentral.com)
- The presence of CMNV in fertilized eggs and larvae indicates that CMNV can transmit vertically via sperm and oocytes in E. carinicauda , which highlights the high probability of vertical transmission of CMNV in the main species of cultured shrimp and prawns. (microbiologyresearch.org)
- Defects in the biogenesis of the acrosome often lead to the impaired development of sperm cells and consequently, to the inability to fertilize the oocyte. (nusl.cz)
- This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
- Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
- ROS will first damage the sperm membrane, decreasing sperm motility and its ability to fuse with the oocyte. (swisscaviarlieri.com)
- It is evident from the following scientific study which is published a in peer-reviewed journal that Caviarlieri can help to reduce oxidative stress and cellular damage, stimulating increase in sperm count and sperm viability. (swisscaviarlieri.com)
- It is evident from the results of this study that the group of mice fed with 10 mg of Caviar DNA (Bioactive Caviar Marine Compound) from Caviarlieri showed a significant increase in Sperm Count (p (swisscaviarlieri.com)
- The acrosomal cap of the head of the sperm breaks down, releasing enzymes that dissolve the zona pellucida locally allowing the spermatozoon to enter the oocyte (Figure 10.4). (pediagenosis.com)
- The results of ISH indicated that the positive signals were evident in the oocytes within ovarian tissue and nauplii. (microbiologyresearch.org)
- A number of tests have been proposed and evaluated that may be used to prognosticate ovarian responsiveness to exogenous gonadotropin stimulation, quality of the oocytes, subsequent implantation and pregnancy rates (PR) (6-9). (ijfs.ir)
- These disorders, which are usually evident by age 35, increase risk of ovarian germ cell cancer. (msdmanuals.com)
- Unlike other mammals, dogs ovulate at first meiotic prophase, and their oocytes mature for 2 to 3 days in the oviduct's distal regions. (nature.com)
- The meiotic spindle in the oocytes of older women frequently exhibits abnormalities in chromosome alignment and microtubular matrix composition (3). (ijfs.ir)
- In addition, we determined significant relationships between AMH and the number of collected oocytes (r=0.38, P=0.01), AMH and the number of metaphase II oocytes (r= 0.35, P=0.01), AMH and the number of embryo (r=0.19, P=0.04) as well as AMH and total dose of the drugs (r=-0.25, P=0.01). (ijfs.ir)
- Although it is evident that the acrosome is an essential structure, its main function has not been solved yet. (nusl.cz)
- This statement is based on evidence that acrosome is full of proteolytic enzymes, and inhibition of these enzymes reduces the ability to fertilize the oocyte. (nusl.cz)
- Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates. (biomedcentral.com)
- This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro . (nature.com)
- SERCA2b and Ca(2+) permeable nicotinic plasmalemmal channels were expressed in oocytes, and hyperpolarizing pulses were delivered to induce Ca(2+) influx and thereby load ER stores. (ox.ac.uk)
- Arachidonic acid (10 μM) potentiated currents through receptors expressing GluR1 and 3 (GluR1,3) to 170% of basal level during initial 20 min following application, being still evident at 60-min washing- out of the drug, while it never or little enhanced currents through receptors expressing GluR1 and 2 (GluR1,2) or GluR1, 2, and 3 (GluR1,2,3) (110% 30 min after treatment). (msu.ru)
- In the assay of intracellular calcium mobilizations, Ca2+ influx in response to receptor activation was greatest with receptors formed in oocytes expressing GluR1,3. (msu.ru)
- For SCNT, the chromosomes of the unfertilized canine oocytes were removed by micromanipulation, and a single donor cell was transferred into each enucleated oocyte. (nature.com)
- Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo . (nature.com)
- In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
- Reduced functioning of the ovaries, the female reproductive organs that produce egg cells (oocytes) and female sex hormones, is also very common. (medlineplus.gov)
- The localization of the bacterium (via light and fluorescence in situ hybridization microscopy) is in the X. pachtaicum females clustered around the developing oocytes, primarily found embedded inside the epithelial wall cells of the ovaries, from where they are dispersed in the intestine. (uibk.ac.at)
- Puffs evoked by photoreleased IP(3) were significantly potentiated in terms of numbers of responding sites, frequency and amplitude following transient Ca(2+) influx in SERCA-overexpressing cells, whereas little change was evident with SERCA overexpression alone or following Ca(2+) influx in control cells not overexpressing SERCA. (ox.ac.uk)
- The peptidoglycan layer is, however, evident only occasionally and not detectable by TEM in most cells. (uibk.ac.at)
- The two cells that result from this division are the definitive oocyte and the second polar body. (pediagenosis.com)
- iOS that ranked honored in the mammalian oocyte regulation methods and protocols drought Below was her of in-depth, first thoughts teaching out at her. (pckltdlaw.com)
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- Pregnancy was established only after embryo transfer of very-early-stage nuclear-transfer constructs (that is, less than 4 hours after oocyte activation). (nature.com)
- this decline in reproductive ability is marked by increased rates of infertility, miscarriages, and birth defects that arise from aneuploidies and generally decreased oocyte quality. (gcrle.org)
- Currently it is not possible for a woman to know where she stands on this scale of age-related oocyte quality decline, as current measures of oocyte quality are both short-range and highly invasive. (gcrle.org)
- Here we propose the development of a non-invasive diagnostic that would predict age-related oocyte quality decline to identify changes that are reflected in aging of reproductive tissues, which will accelerate our ability to both study and treat reproductive aging. (gcrle.org)
- The age-associated decline in female fecundity as well as increased risk for spontaneous abortion, are largely attributable to abnormalities in the oocyte. (ijfs.ir)
- This reduction in fecundity was accompanied by a significant increase in the incidence of oocyte atresia along with a significant increase in the accumulation of oocyte debris in the oviduct. (europa.eu)
- Furthermore, there are no concentration-response relationships evident in the study by Moreels et al (2006b), no historical database comparison to give further information on normal biological ranges and limited information on the procedure elements of the measurements. (europa.eu)