• All other groups showed comparatively high basal expression levels and moderate heat-inducibility, signifying constitutively expressed genes. (biomedcentral.com)
  • Furthermore, based on differentially expressed genes and WGCNA analyses, we found that the hypoxia signaling pathway was enriched under heat shock conditions. (biomedcentral.com)
  • Heat shock proteins (HSPs) are highly conserved and immunogenic proteins that are shared among diverse groups of mammals and microbial agents. (eg.net)
  • Heat shock proteins (HSPs) are a highly conserved family of proteins critical to both environmental stress response and cellular function under normal conditions. (charlotte.edu)
  • Throughout evolution, eukaryotes adopted a network of highly conserved proteins that buffer the positive charges of histones, maintaining their solubility and, therefore, avoiding aberrant interactions with other cellular components. (aging-us.com)
  • One important heat shock protein is discussed further in the blog entry HSP70 to the rescue The relatively recent blog entries Mitohormesis and Radiation hormesis define hormesis in more detail and discuss it as it relates to mitochondrial oxidative stress and stress induced by radiation. (anti-agingfirewalls.com)
  • Therefore, we have characterised the Hsp70 gene family of Paramecium caudatum to gain insight into the evolution and differential heat stress response of the distinct family members in protists and to investigate the diversification of eukaryotic hsp70 s focusing on the evolution of heat-inducibility. (biomedcentral.com)
  • These treatments induced exceptionally high mRNA up-regulations in one cytosolic group with a low basal expression, indicative for the major heat inducible hsp70 s. (biomedcentral.com)
  • Our analyses suggest an independent evolution of the heat-inducible cytosol-type hsp70 s in Paramecium and in its close relative Tetrahymena , as well as within higher eukaryotes. (biomedcentral.com)
  • This result indicates convergent evolution during hsp70 subfunctionalization and implies that heat-inducibility evolved several times during the course of eukaryotic evolution. (biomedcentral.com)
  • Prokaryotic Hsp70 (DnaK) proteins share about 50% amino acid identity with eukaryotic Hsp70s. (biomedcentral.com)
  • The co-chaperone Hep1 is required to prevent the aggregation of mitochondrial Hsp70 proteins. (cipsm.de)
  • and HSPA6 (heat shock protein family A (Hsp70) member 6). (nih.gov)
  • In other animals, heat shock response is a transcriptional response driven by the heat shock transcription factor 1 ( HSF1 ) and thermal stress can trigger HSP70 expression to protect the organism via HSF1 . (biomedcentral.com)
  • In the upregulated genes, we identified one HSP70 potentially regulated by HSF1 in response to heat shock. (biomedcentral.com)
  • Our results revealed that the expression of HSP70 and HSP20 was initially triggered after 2 h of heat shock, and one of the HSP70 genes was potentially regulated by HSF1 . (biomedcentral.com)
  • Prokaryotic and eukaryotic organisms respond to heat shock or other environmental stress by inducing the synthesis of proteins collectively known as heat-shock proteins (hsp). (wikipedia.org)
  • We have previously produced the recombinant AMP-17 protein in a prokaryotic expression system and showed that it possesses excellent antifungal activity (Guo et al. (researchsquare.com)
  • We also did massive analysis of domain profiles in that GOS dataset and explored the unexpected diversity of protein kinase-like proteins in prokaryotic genomic and metagenomic data. (manninglab.org)
  • However, the fact that OsClpC3 protein appears mitochondrial/chloroplastic with equal probability and OsClpC4 a plasma membrane protein reflects functional diversity of this class. (biomedcentral.com)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • Mitochondrial dna replication, mrna transcription, and protein translation occur independently, including the synthesis of polypeptides encoded. (caisouthern.com)
  • The first is that ATP production, coupled to electron transport, and translation of mitochondrial proteins represent the essence of mitochondrial function: these functions are common to all mitochondrial genomes and can be traced unambiguously and directly to an α-proteobacterial ancestor. (biomedcentral.com)
  • The pore-forming BCL-2 family proteins mediate mitochondrial poration to initiate apoptosis through protei-protein and protein-lipid interactions. (portlandpress.com)
  • Additionally, the crosstalk between protein synthesis and proteasome-mediated protein degradation was analyzed in eukaryotic organisms under various cellular conditions. (diva-portal.org)
  • Protein synthesis inhibition was observed to increase proteasome function and assembly in human primary embryonic fibroblasts, with heat shock protein chaperone machinery to contribute to the elevated proteasome assembly. (diva-portal.org)
  • Alternatively, protein synthesis inhibition increased the protein levels of specific proteasome subunits without influencing the proteasome activity in C. elegans. (diva-portal.org)
  • Furthermore, proteasome activation by means which have also pro-longevity effects decreased the protein synthesis rate both in human fibroblast cellsand nematodes. (diva-portal.org)
  • Generally, there is a transient increase in HSPs synthesis at a low level of temperature elevation, with a more sustained response observed at higher temperatures, a pattern of response observed in numerous organisms. (eg.net)
  • In all living cells, protein synthesis occurs on ribonucleoprotein particles called ribosomes. (cipsm.de)
  • The environmental stress response in all organisms as diverse as pro- and eukaryotes is generally coupled with a remarkable change in gene expression patterns and an enhanced synthesis of several 'stress proteins' [ 1 ]. (biomedcentral.com)
  • The genetic code is a system of specific base sequences that specify which amino acids are to be used for the synthesis of a protein during translation. (caisouthern.com)
  • Protein synthesis is the process of creating protein molecules. (caisouthern.com)
  • Dna is housed within the nucleus, and protein synthesis takes place in the cytoplasm, thus there must be some sort of intermediate messenger. (caisouthern.com)
  • Protein synthesis is tightly regulated at each step of translation. (caisouthern.com)
  • Protein synthesis is a process of synthesizing proteins in a chain of amino acids known as polypeptides. (caisouthern.com)
  • The proteostasis network (PN) is an assembly of distinct dynamic molecular pathways that control the functionality of the proteome (proteome homeodynamics) during protein synthesis, folding, trafficking, and degradation. (hindawi.com)
  • This is due to a paucity of functional annotation from sequence homology, which is compounded by low genetic tractability compared with many model organisms. (portlandpress.com)
  • We are using comparative genomics to explore cellular machinery and transcriptional responses across major model organisms. (manninglab.org)
  • Members of the Alb3/Oxa1/YidC protein family function as insertases in chloroplasts, mitochondria, and bacteria. (cipsm.de)
  • Using the respective signal sequences cloned upstream to GFP/CFP reporter proteins and transient expression studies with onion epidermal cells, evidence is provided that rice ClpB-m and Clp-c proteins are indeed localized to their respective cell locations mitochondria and chloroplasts, respectively. (biomedcentral.com)
  • Is the amitochondrial condition in these organisms a secondary adaptation or is it evolutionarily primitive - or, in other words, did any organisms diverge from the main line of eukaryotic evolution before the advent of mitochondria? (biomedcentral.com)
  • Whereas the issue of how the eukaryotic cell arose remains controversial [ 8 , 9 ], current genomic data do allow us to make a number of reasonably compelling inferences about how mitochondria themselves originated and have since evolved. (biomedcentral.com)
  • Mitochondria are highly dynamic organelles that provide essential metabolic functions and represent the major bioenergetic hub of eukaryotic cell. (hindawi.com)
  • Mitochondria are the energy producing organelles in eukaryotic cell providing ATP through oxidative phosphorylation (OXPHOS). (hindawi.com)
  • The mitochondria have their own chaperones and proteolytic enzymes that remove damaged or unfolded proteins [ 18 - 20 ]. (hindawi.com)
  • Mitochondria are essential for eukaryotic life, because they provide most of the energy for a variety of cellular processes. (uni-goettingen.de)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)
  • The microbial assemblages of marine organisms play fundamental biological roles in their eukaryotic hosts. (charlotte.edu)
  • In contrast, one protein forms various structures and implements other biological phenomena depending on the situation. (springeropen.com)
  • Our review presents a description of the components of supramolecular protein assembly and their application in understanding biological phenomena to therapeutics. (springeropen.com)
  • Lastly, we speculate the biological phenomena from organism and their application with their own properties. (springeropen.com)
  • Biological weapons include any organism or toxin found in nature that can be used to incapacitate, kill, or otherwise impede an adversary. (medscape.com)
  • The biological function of the differentially expressed circRNAs was determined by protein interaction network, Gene Ontology (GO), and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment. (biomedcentral.com)
  • We found 1100 differentially expressed circRNAs (675 downregulated and 425 upregulated) which were involved in various biological processes such as protein ubiquitination modification, cell cycle regulation, RNA transport, and autophagy. (biomedcentral.com)
  • During the infection Borrelia burgdorferi migrate into different tissues of the host organism to reach so-called "immune privileged niches" where they are protected from the attack of the immune system. (altmeyers.org)
  • These seem to act as protein chaperones that can protect other proteins against heat-induced denaturation and aggregation. (wikipedia.org)
  • Evaluation of the antiaging and protein anti-aggregation effects of this bioactive compound indicated that 18α-GA promoted longevity in nematodes through proteasome-and SKN-1-mediated activation and decelerated Alzheimer'sdisease progression and neuropathology both in nematodes and neuronal cells. (diva-portal.org)
  • This thesis suggests: 1) that a diet-derived compound could act as a pro-longevity and anti-aggregation agent in the context of amulticellular organism and 2) the existence of a complex interplay between anabolic and catabolic processes under different cellular conditions, across species. (diva-portal.org)
  • The holdase function is reliably and easily achieved by monitoring the suppression of heat-induced aggregation of well-characterized model protein substrates. (bvsalud.org)
  • Using DnaK from Escherichia coli and heat-induced aggregation of malate dehydrogenase, we describe a protocol for absorbance and fluorescence-based miniaturized versions of the standard aggregation suppression assay that are affordable and have wide application for low abundance holdases. (bvsalud.org)
  • These sequences represent the protein coding region of the dnajb11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • These proteins bind to specific DNA sequences in any organism and introduce cuts. (vectorbuilder.cn)
  • The clinical C31 online artificial coverage treatments enriched for time Integrate have aimed from the Eukaryotic transgenic vertebrate scan Purification accuracy HEK293 and CHO microRNAs, which are from artifacts of the observed Hamster. (scoutconnection.com)
  • This process allows a variety of important applications, including the study of gene regulation, the analysis of the expression of proteins within eukarotic cells such as those of mammals, the production of transgenic organisms , and the methods of gene therapy. (newworldencyclopedia.org)
  • Proteasome degradation machinery is responsible for the turnover of a huge variety of normal and abnormal proteins, thus regulating a plethora of cellular processes. (diva-portal.org)
  • If the coding sequence of the protein is unknown but the protein is purified, mass spectrometry (or chemical methods such as Edman degradation) can be used to determine the amino acid sequence of the protein, which can then be used to search for the the corresponding DNA sequence. (hideyuki-ts.com)
  • Along with PTMs, the partial or complete disassembly of nucleosomes allows the exchange and degradation of pre-existing histone proteins, with the incorporation of newly synthesised histones onto chromatin that can eventually result in the resetting of previous epigenetic marks. (aging-us.com)
  • Chloroplast-located proteins which are encoded by the nuclear genome have to be imported from the cytosol into the organelle in a post-translational manner. (cipsm.de)
  • This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. (maayanlab.cloud)
  • Protein-protein interactions (PPI) in cells play a pivotal role in cellular function and dynamics. (bvsalud.org)
  • Heat-shock proteins of the 70 kDa family (Hsp70s) are essential chaperones required for key cellular functions. (biomedcentral.com)
  • Extensive research on Hsps revealed also a constitutive expression of some members of these proteins, suggesting that they are also essential in maintaining the cellular functions under normal physiological conditions. (biomedcentral.com)
  • In addition to dedicated duplex-DNA-binding domains, cellular initiators possess AAA+ elements that drive features ranging from protein assembly to origin recognition. (justdoitsnow.com)
  • Cells express a pool of thousands of different proteins that need to be tightly controlled for proper cellular structure, organization, and function. (hindawi.com)
  • cellular components containing CCT8 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset. (maayanlab.cloud)
  • cellular components containing CCT8 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset. (maayanlab.cloud)
  • cellular components co-occuring with CCT8 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset. (maayanlab.cloud)
  • Extreme thermal events are increasing in frequency and duration as the climate continues to warm, with potential detrimental effects on marine organisms. (frontiersin.org)
  • A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. (lookformedical.com)
  • In this paper, we will discuss about the component of supramolecular protein assembly and classify their conformation according to structural dimensionality. (springeropen.com)
  • then, introns distributed to joint online artificial photosynthesis 2016 integrase like the Helmholtz Protein Sample Production Facility( PSPF) have a longitudinal site of blunt mortality Carriers obtaining changes, structural construct survival and the embodiment efficiency RRS exchange( BEVS). (scoutconnection.com)
  • CCT, chaperonin containing TCP-1), uses a built-in lid to mediate protein folding in an enclosed central cavity. (cipsm.de)
  • Thus, GCGs contain proteins involved in translation initiation, translation control, and mRNA decay, which is consistent with their proposed role in the regulation ofs maternal mRNA expression. (rupress.org)
  • This panel should contain proteins such as Osp C, BmpA (Borrelia membrane antigen = p39 antigen), p41 antigen and p100 antigen. (altmeyers.org)
  • This assay allows for the distribution and spatiotemporal analysis of HSP90-CDK4 complexes in live or fixed cells and is amenable to studying the effects of inhibitors and mutations on chaperone-client protein networks. (bvsalud.org)
  • We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. (cipsm.de)
  • protein complexs containing CCT8 protein from the CORUM Protein Complexes dataset. (maayanlab.cloud)
  • Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
  • An emerging field proposes that organismal aging is caused in part by a decline in proteostasis - the cell's ability to keep proteins folded and functional, through chaperones and other mechanisms. (manninglab.org)
  • Hsp20, like all heat shock proteins, is in abundance when cells are under stressed conditions. (wikipedia.org)
  • In the present thesis, 18α-glycyrrhetinic acid (18α-GA), a natural compound with known proteasome activating properties in cells, was indicated to activate proteasome also in the multicellular organism Caenorhabditis elegans (C. elegans). (diva-portal.org)
  • An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. (lookformedical.com)
  • Consequently, strategies to visualize and analyze PPI in cells are useful in understanding protein homeostasis regulation. (bvsalud.org)
  • The Bimolecular Fluorescence Complementation (BiFC) assay has emerged as a useful tool for studying PPI between proteins in live or fixed cells. (bvsalud.org)
  • After the pathogenic bacterium Legionella pneumophila is phagocytosed, it injects more than 250 different proteins into the cytoplasm of host cells to evade lysosomal digestion and to replicate inside the host cell. (cipsm.de)
  • From the complementation assays, OsClpD1 appears to be more effective than OsClpB-cyt protein in rescuing the thermosensitive defect of the yeast ScΔhsp104 mutant cells. (biomedcentral.com)
  • When the rare tRNAs are depleted to produce the recombinant protein, proliferation of the host cells is restricted, leading to low yield. (hideyuki-ts.com)
  • As scaffolding molecules, they significantly regulate the DNA packaging into the nucleus of all eukaryotic cells. (aging-us.com)
  • Eukaryotic cells compact their large genome into highly ordered chromatin structures within the nucleus. (aging-us.com)
  • Circular RNAs (circRNAs) belong to a class of non‐coding RNAs that are widespread in the cytoplasm of eukaryotic cells and are structurally and functionally different from linear RNA molecules [ 1 ]. (biomedcentral.com)
  • Commonly, the term transfection is used in the sense of non-viral introductions of nucleic acid specifically into eukaryotic cells, or even more specifically into animal cells. (newworldencyclopedia.org)
  • It now includes any means of artificial introduction of foreign DNA into cells, or more specifically cultured eukaryotic cells. (newworldencyclopedia.org)
  • Transformation of eukaryotic cells in tissue culture is usually called transfection. (newworldencyclopedia.org)
  • Instead, a transformation technique is utilized, where competent bacterial cells are made temporarily permeable due to brief shock using either heat or electricity. (vectorbuilder.cn)
  • These include electroporation, where cells are shocked and become permeable to plasmid DNA, and lipofection, where plasmids are contained within a lipid bilayer which merges with the host cell. (vectorbuilder.cn)
  • Protein Kinases are one of the largest gene families in eukaryotes, covering about 2% of most eukaryotic proteomes, and with about 60 kinase families shared between most eukaryotes. (manninglab.org)
  • While expression of OsClpB-m was significantly less as compared to its cytoplasmic and chloroplastic counterparts in different tissues, this transcript showed highest heat-induced expression amongst the 3 ClpB proteins. (biomedcentral.com)
  • Body temperature is determined by the balance between heat production by tissues, particularly the liver and muscles, and heat loss from the periphery. (msdmanuals.com)
  • especially, in localization, Nonetheless small lipid requires purified digested to the protein of the most TSLC1 random expression to be the two figures. (scoutconnection.com)
  • Ageing leads to a gradual dysfunction of the proteostasis network and thus to proteome instability due to accumulation of damaged and/or misfolded proteins [ 2 ]. (hindawi.com)
  • Xenopus tropicalis DnaJ heat shock protein family (Hsp40) member B11 (dnajb11), mRNA. (genscript.com)
  • Some of the most important and well investigated Hsps are the members of the 70 kDa heat-shock protein family (Hsp70s). (biomedcentral.com)
  • From these results, it is evident that not all heat-inducible genes were triggered simultaneously in response to heat shock stress. (biomedcentral.com)
  • Hsp20 proteins seem to form large heterooligomeric aggregates. (wikipedia.org)
  • Hsp20 has been studied extensively in cardiac myocytes and is known to act as a chaperon protein, binding to protein kinase 1 (PDK1) and allowing its nuclear transport. (wikipedia.org)
  • Due to hsp20 commonly forming dimers with itself when heated, its function of chaperoning can be greatly affected. (wikipedia.org)
  • The redox swap in eukaryotic DNA primases appears to control polymerase handoff, and in DNA polymerase δ, the redox change supplies a means to modulate replication in response to oxidative stress. (justdoitsnow.com)
  • Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues. (biomedcentral.com)
  • Of these 9, only 3 belonging to ClpB group are heat stress regulated. (biomedcentral.com)
  • Heat stress threatens future prospects of increased grain production in crops. (biomedcentral.com)
  • Induction of stress proteins by heat shock and metal ions l. (caisouthern.com)
  • ABA is a stress hormone perceived by the soluble receptor PYR1 (encoded by Pyrabactin resistance 1 ) and its related PYR1- like and R egulatory C omponent of ABA R ecepto r proteins ( PYL/RCAR , PYLs for simplicity) 18 . (nature.com)
  • There were abundance differences in proteins associated with stress, photosynthesis, carbohydrate and lipid metabolism proteins. (biomedcentral.com)
  • These differences in protein abundance have given greater understanding of the mechanism by which salt stress promotes fatty acid accumulation in the un-sequenced microalga C. nivalis as it switches to a non-growth state, whereas C. reinhardtii does not have this response. (biomedcentral.com)
  • Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. (biomedcentral.com)
  • Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)
  • HSP100 is a major heat-regulated protein family in diverse organisms. (biomedcentral.com)
  • Crystal structures often deviate from the natural system: Proteins, especially membrane-bound ones, do not necessarily crystallize in their biologically active structures and the measures needed to obtain suitable GPCR crystals tend to increase the diversity between the natural environment and the crystal. (gauss-centre.eu)
  • We've worked in the past on a few aspects of metagenomics: one was to use protein sequence similarity to assemble metagenomes and understand their diversity. (manninglab.org)
  • In particular, the formation of the basic cap-binding complex, eukaryotic initiation. (caisouthern.com)
  • Translation across the human genome reveal that isoforms from the same gene can differ in protein production by two orders of magnitude. (caisouthern.com)
  • Explore how a protein is made from an mrna sequence. (caisouthern.com)
  • Genes encode proteins, and the instructions for making proteins are decoded in two steps: first, a messenger rna (mrna) molecule is produced through the. (caisouthern.com)
  • Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)
  • An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. (lookformedical.com)
  • They could adjust themselves well to the harsh environment of the textile effluent because of two reasons (1) they are capable of synthesizing the heat shock proteins (2) their ability to synthesize a wide variety of enzymes. (scialert.net)
  • The vesicle-inducing protein in plastids 1 (Vipp1) is an essential component for thylakoid biogenesis in cyanobacteria and chloroplasts. (cipsm.de)
  • Associated with their diverse cell locations, domain structures of OsClpB-c, OsClpB-m and OsClpB-cyt proteins are noted to possess a high-level conservation. (biomedcentral.com)
  • The basic principle that forms these hierarchical structures is protein self-assembly. (springeropen.com)
  • Supramolecular assembly is a very common phenomena in nature and these natural supramolecular proteins have various structures, from simple structures to complex structures [ 1 , 2 ]. (springeropen.com)
  • however, more protein structures have been identified by advanced technology (Fig. 1 ). (springeropen.com)
  • A better understanding of the proteins contributes to designing new supramolecular structures from rational design to de novo design. (springeropen.com)
  • Recently, the de novo design of proteins that start from scratch can create a new topology beyond experimentally determined structures. (springeropen.com)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
  • Protein expression is influenced by many factors that may vary between experiments or laboratories. (genscript.com)
  • Outline an expression and purification strategy, using both traditional and high-throughput methods, to produce a sample suitable for protein crystallography. (hideyuki-ts.com)
  • The expression and purification strategies depend on the characteristics of the protein of interest, such as size, shape, intrinsic solubility, requirement of post-translational modifications and/or cofactors, presence of disulfide bridges, in vitro stability. (hideyuki-ts.com)
  • The structure of some homologous proteins might have been solved previously and we can refer to their expression and purification strategies, which might prevent some waste of time on trial-and-errors. (hideyuki-ts.com)
  • The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. (researchgate.net)
  • We identified 150 genes responsive to heat shock including seven HSP genes, six of which belonging to the group of 17 HSP genes enriched in response to heat shock, according to weighted gene co-expression network analysis (WGCNA). (biomedcentral.com)
  • The inventors have already established a system to express an L-lactate dehydrogenase gene using an original promoter function of the foreign PDC1 gene and exclude the expression of PDC1 protein that is expressed by the original foreign promoter, by linking the objective valuable gene (L-lactate dehydrogenase gene in this case) in the downstream of chromosomal PDC1 promoter and disrupting the yeast chromosomal PDC1 gene simultaneously. (justia.com)
  • A major focus is in three transcriptional responses - to heat shock, insulin signaling, and dietary restriction - that both modulate aging and may affect neurodegeneration. (manninglab.org)
  • Here, we describe the application of the BiFC assay to a model of chaperone-client interactions using Hsp90 and the validated client protein CDK4. (bvsalud.org)
  • Involved in heat shock-mediated polytene chromosome puffing and response to hypoxia. (nih.gov)
  • The gene, for culprit protein, MeCP2 (methylated CpG binding protein) is located on chromosome X and the majority of patients are young girls. (uni-goettingen.de)
  • The basic purpose of hormesis is maintaining a health condition of homeostasis or better in a cell, organ or entire organism. (anti-agingfirewalls.com)
  • Distribution of ClpB proteins to different cell organelles indicates that their functioning might be critical in different cell locations. (biomedcentral.com)
  • Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
  • An example for the most primitive form of eukaryotic system is the fungal cell. (scialert.net)
  • These debates have posed such questions as the following: Did the mitochondrion arise at the same time as, or subsequent to, the rest of the eukaryotic cell? (biomedcentral.com)
  • The first described CID systems involve microbe-derived dimerizing ligands, such as rapamycin, that direct protein neo-associations that rewire eukaryotic cell signaling 5 . (nature.com)