AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Expressed Sequence TagsMolecular Sequence DataBase SequenceRNA SplicingAmino Acid SequenceBinding SitesGene LibraryIntronsSequence Analysis, DNARNA PrecursorsRibonucleoproteins, Small NuclearIsomerasesSequence Homology, Nucleic AcidDNA, ComplementaryContig MappingExonsSequence AlignmentRNA Splice SitesRibonucleoprotein, U2 Small NuclearConsensus SequenceSubstrate SpecificityProtein Disulfide-IsomerasesCloning, MolecularRNA, Small NuclearSpliceosomesAlternative SplicingRNA, MessengerConserved SequenceSequence Homology, Amino AcidTranscription, GeneticGene Expression ProfilingEscherichia coliThioredoxinsSequence Tagged SitesRibonucleoproteinsDatabases, GeneticPhylogenyMutationGenome, PlantProtein BindingNucleic Acid ConformationKineticsHeLa CellsPolymerase Chain ReactionGenes, PlantPromoter Regions, GeneticDNAOpen Reading FramesPlasmidsDNA-Binding ProteinsChromosome MappingMutagenesis, Site-DirectedTranscription FactorsDNA PrimersDatabases, Nucleic AcidRegulatory Sequences, Nucleic AcidComputational BiologyRepetitive Sequences, Nucleic AcidRestriction MappingCell LineModels, GeneticDNA, PlantRecombinant ProteinsDNA Mutational AnalysisRadiation Hybrid MappingDNA Transposable ElementsGenomicsDNA, BacterialEndopeptidasesModels, MolecularChickensOligodeoxyribonucleotidesPeptide FragmentsDNA, ViralCluster AnalysisMolecular WeightRNAMutagenesisMicrosatellite RepeatsMolecular Sequence AnnotationStructure-Activity RelationshipPeptidesGene Expression Regulation, PlantOligonucleotide Array Sequence AnalysisProtein ConformationCicerRNA, ViralNuclear ProteinsProtein Structure, TertiarySaccharomyces cerevisiaeGenetic MarkersElectrophoresis, Polyacrylamide GelSoftwareRNA, PlantViral ProteinsDatabases, FactualTranscriptomeVirus ReplicationBacterial ProteinsLens Plant
Genome sequenceGenesCDNA clonesGenomic sequencesWhole genomeCDNAsGenbank DatabaseProteinSSRsNucleotidesPolymeraseClonesChromosomesAccession2002ConsortiumCentersMRNAJaponicaFragmentRiceIncompleteMicrobialProteinsAlignmentsMotifsPredictionNext-generationDataMolecular BiologyLociIdentificationBlastPubliclyFunctional genomicsGenerateTechnologyTranslationMarkersCompleteHighIntegrateMethodsFullyContentReal estateSourceProjectLength
Genome sequence6
  • 1998) Genome sequence of the nematode C. elegans: a platform for investigating biology. (wikipedia.org)
  • We will be looking at working towards the $100,000 genome sequence landmark in 2007. (outsourcing-pharma.com)
  • Bulk submissions of Expressed Sequence Tag (EST), Sequence Tagged Site (STS), Genome Survey Sequence (GSS), and High-Throughput Genome Sequence (HTGS) data are most often submitted by large-scale sequencing centers. (iospress.nl)
  • The NCBI Datasets Command Line Interface (CLI) tools provide easy, straightforward programmatic downloads of assembled genome sequence data. (nih.gov)
  • In the absence of a finished quality genome sequence, insights into wheat genome structure and function have been accumulating through survey sequencing of individual chromosomes or chromosome-specific Bacterial Artificial Chromosome (BAC) libraries. (biomedcentral.com)
  • Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
Genes13
  • We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)
  • The use of expression levels of transcription factor-encoding genes according to hierarchical anatomical classifications covering different tissues and cell types makes it possible to filter out irrelevant binding site predictions and to identify candidates of potential functional importance for further experimental testing. (lu.se)
  • Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat (NBS-LRR) resistance genes ( R ) have been identified, but a small portion with expressed transcripts has been found. (jipb.net)
  • The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. (jipb.net)
  • A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. (jipb.net)
  • These expressed RGAs are the source for RGA-tagged marker development and identification of peanut resistance genes. (jipb.net)
  • incollection{RaeSon04, author = {R\"atsch, G. and Sonnenburg, S.}, pages = "277-298", title = "Accurate Splice Site Prediction for Caenorhabditis Elegans", publisher = "MIT Press", year = "2004", series = "MIT Press series on Computational Molecular Biology", abstract = "We propose a new system for predicting the splice form of Caenorhabditis elegans genes. (tu-berlin.de)
  • As a first step we generate a clean set of genes from available exressed sequence tags (EST) and complete complementary (cDNA) sequences. (tu-berlin.de)
  • From all such genes we then generate potential acceptor and donor sites as they would be required by any gene finder. (tu-berlin.de)
  • 293 rice sequences are full length (from both subspecies)I will be trying to name all the rice genes before August 20. (uthsc.edu)
  • The transcript profiles of uterine tissue were compared in treated versus control in both maternal and neonatal sites using cDNA microarray to determine the expression levels of approximately 13,000 genes and expressed sequence tags (ESTs). (diethylstilbestrol.co.uk)
  • Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
  • Aspergillus flavus expressed sequenced tags (ESTs) provides a rapid and effective method for identification of the genes involved in aflatoxin formation and promises effective control strategy being developed through biotechnology. (usda.gov)
CDNA clones3
  • Single pass sequencing of cDNA clones provides a rapid route to gene discovery and production of EST's (2). (le.ac.uk)
  • Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for conifer biology. (le.ac.uk)
  • More than 26,110 cDNA clones have been sequenced. (usda.gov)
Genomic sequences2
  • The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)
  • Despite the advent of next-generation sequencing technologies, the above mentioned attributes of the wheat genome have rendered the assembly of genomic sequences extremely difficult. (biomedcentral.com)
Whole genome2
  • The system is capable of expressed sequence tag (EST) sequencing, the identification of small RNAs and transcription binding sites as well as whole genome sequencing and metagenomics. (outsourcing-pharma.com)
  • One of the things that is really important for high throughput DNA sequencing is for people to see that researchers can look at the whole genome of bacteria and viruses. (outsourcing-pharma.com)
CDNAs2
  • Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)
  • If the cDNAs contain translation initiation sites, they may be suitable for functional genomics studies. (umn.edu)
Genbank Database1
  • I have done systematic searches of the Genbank Database for rice P450 ESTs, GSSs, sequences in nr and HTGS hits. (uthsc.edu)
Protein8
  • The site now also provides tools for viewing protein and metabolic pathways, and a comparative genomics platform that can aid in nematode drug, vaccine and pesticide research. (wikipedia.org)
  • NemaBLAST - a tool allowing the alignment of user defined protein or nucleotide sequence against nematode transcripts or cDNA reads organized by organism or library. (wikipedia.org)
  • The GenBank sequence database is an annotated collection of all publicly available nucleotide sequences and their protein translations. (iospress.nl)
  • On June 20th, we'll show you how to see the genomic context of bacterial proteins using the identical protein report and the graphical sequence viewer. (nih.gov)
  • Entrez is NCBI's primary text search and retrieval system that integrates the PubMed database of biomedical literature with 38 other literature and molecular databases including DNA and protein sequence, structure, gene, genome, genetic variation and gene expression. (nih.gov)
  • Conserved Domains is a database of protein domains represented by sequence alignments and profiles for protein domains conserved in molecular evolution. (nih.gov)
  • On one hand, codon redundancy (i.e. that 61 codons specify 20 amino acids [ 21 ]) potentially allows a wide variety of mRNA sequences carrying different codons to specify the same protein product. (biomedcentral.com)
  • As a consequence, the frequent use of codons matching common (i.e. 'preferred codons') or rare aminoacyl-tRNA species can lead to different levels of protein production, as delays or pauses in translation are likely to occur at rare codon sites. (biomedcentral.com)
SSRs3
  • Twenty-eight simple sequence repeats (SSRs) were identified from 25 peanut expressed RGAs. (jipb.net)
  • Co-dominant simple sequence repeat (SSRs) are popular DNA markers, widely applied in oil palm genetic studies, especially in genetic mapping, marker-trait association, diversity analysis and detecting illegitimacy. (mpob.gov.my)
  • This study also characterised a large number of expressed sequence tags (EST)-SSRs using an in-silico approach. (mpob.gov.my)
Nucleotides3
  • If you sequence more than one nucleotide at once you get a stronger signal and we see a very linear response between the signal strength and the number of nucleotides added. (outsourcing-pharma.com)
  • Background: Expressed Sequence Tag (EST) sequences are generally single-strand, single-pass sequences, only 200-600 nucleotides long, contain errors resulting in frame shifts, and represent different parts of their parent cDNA. (umn.edu)
  • Future research programs can be focused on the development of transgenic plants with enhanced stress tolerance in field conditions based upon the outcome of genomic approaches and knowing the mystery of nucleotides sequences hidden in cells. (intechopen.com)
Polymerase1
  • During the nucleotide flow each of the beads is sequenced in parallel, with the polymerase extending the sequencing strand only if the nucleotide is complimentary to the template strand. (outsourcing-pharma.com)
Clones5
  • What primer should I use to sequence MXO clones? (indiana.edu)
  • Exelixis provided BDGP and Flybase with their EST sequences, but the clones were discarded because a majority were redundant with the BDGP EST collection. (indiana.edu)
  • Where can I find out more about my clones (restrictions sites, sequence, etc. (indiana.edu)
  • How do I find maps and sequence information on the Gateway clones (Terence Murphy collection)? (indiana.edu)
  • These GOLD clones can be found in the DGC or EST collections - please enter your clone ID through the DGC or EST collection page and place your order. (indiana.edu)
Chromosomes3
  • An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
  • Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
  • The construction of BAC-based physical maps of individual chromosomes reduces the complexity of this allohexaploid genome, enables elucidation of gene space and evolutionary relationships, provides tools for map-based cloning, and serves as a framework for reference sequencing efforts. (biomedcentral.com)
Accession3
  • Upon receipt of a sequence submission, the GenBank staff assigns an Accession number to the sequence and performs quality assurance checks. (iospress.nl)
  • Assembly provides versioned accession identifiers for submitted and RefSeq assemblies, links to the components in the Nucleotide system and direct access to the downloads on the NCBI FTP site. (nih.gov)
  • 4 We screened the coding sequence of ESPN (GenBank accession number AL136880) by sequencing PCR amplified ESPN exons from genomic DNA of affected individuals in the two families. (bmj.com)
20023
  • 1111, Yata, Mishima, Shizuoka 411-8540, Japan REFERENCE 2 AUTHORS Mishima,H., Shizuoka,T. and Fuji,I. TITLE Glyceraldehyde-3-phosphate dehydrogenase expressed in human liver JOURNAL Unpublished (2002) COMMENT Human cDNA sequencing project. (nig.ac.jp)
  • Sept. 25, 2002, There are 458 different P450 gene sequences in rice. (uthsc.edu)
  • August 9, 2002, 875 total rice contigs, 341 are japonica, 534 are indica 250 japonica sequences are full length. (uthsc.edu)
Consortium1
Centers2
  • The first Genome Sequencer FLX systems were delivered to evaluation sites in November 2006, including major genome centers. (outsourcing-pharma.com)
  • GenBank is built by direct submissions from individual laboratories, as well as from bulk submissions from large-scale sequencing centers. (iospress.nl)
MRNA1
  • Positions Contents --------- -------- 01-05 'LOCUS' 06-12 spaces 13-28 Locus name 29-29 space 30-40 Length of sequence, right-justified 41-41 space 42-43 'bp' 44-47 spaces 48-54 DNA, RNA, mRNA, rRNA, tRNA or cRNA, left justified 55-55 space 56-63 'linear' followed by two spaces, or 'circular' 64-64 space 65-67 The division code (see '3.1. (nig.ac.jp)
Japonica1
  • The study began with the isolation of an apparent homolog of the vertebrate gene Islet from an EST (expressed sequence tag) library for the planarian species Dugesia japonica , which is indigenous to Japan. (riken.jp)
Fragment1
  • These fragments are then ligated by two short adaptors, which provide primers for both amplification and sequencing of the fragment as well as a biotin tag that immobilises it onto a streptavidin-coated bead. (outsourcing-pharma.com)
Rice1
  • A blast server for the rice P450 sequences is available. (uthsc.edu)
Incomplete3
  • Conclusions: ATGpr demonstrates high sensitivity, specificity, and overall accuracy in identifying start sites while also rejecting incomplete sequences. (umn.edu)
  • The BioProject database is a searcheable collection of complete and incomplete (in-progress) large-scale molecular projects including genome sequencing and assembly, transcriptome, metagenomic, annotation, expression and mapping projects. (nih.gov)
  • There are now 202 full length P450s, 61 pseudogene fragments and 61 incomplete sequences. (uthsc.edu)
Microbial1
  • The GenBank direct submissions group also processes complete microbial genome sequences. (iospress.nl)
Proteins4
  • The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). (rndsystems.com)
  • While we could place a number of these peptides within proteins that had already been identified, there were many cysteine-rich peptides that traced to fragments of avian nucleic acid sequences that have yet to find their proper place in the chicken genome. (udel.edu)
  • Different proteins were known to have unique sequences, whereas it was thought that all DNA molecules have the same sequence. (proprofs.com)
  • Grouping proteins into sequence-based clusters is a fundamental step in many bioinformatic analyses ( e.g. , homology-based prediction of structure or function). (biomedcentral.com)
Alignments1
  • Computer programs for the generation of multiple sequence alignments such as "Clustal W" allow detection of regions that are most conserved among many sequence variants. (biomedcentral.com)
Motifs1
  • We cannot say whether these CREMP modules are sometimes paired with other sequence motifs. (udel.edu)
Prediction2
  • A database of EST sequences suitable for validating programs for translation initiation site prediction is now available. (umn.edu)
  • These tools and materials may open an avenue for future improvements in start site prediction and EST analysis. (umn.edu)
Next-generation2
  • Martin J, Abubucker S, Heizer E, Taylor CM, Mitreva M. (2012) Nematode.net update 2011: addition of data sets and tools featuring next-generation sequencing data. (wikipedia.org)
  • Genotyping by sequencing (GBS) is an improved strategy for addressing a complex genome via next-generation sequencing technology. (biomedcentral.com)
Data2
  • We have compared five methods to predict translation initiation sites in EST data: first-ATG, ESTScan, Diogenes, Netstart, and ATGpr. (umn.edu)
  • Notice for patent related sequence data 4. (nig.ac.jp)
Molecular Biology1
  • page: All Methods remain been not to this experiment From: sites in Molecular Biology, Vol. 164: Kinesin Protocols Edited by: I. A) Schematic research of the materials taken and carried for these Polymorphisms. (swcomsvc.com)
Loci1
  • The deafness segregating in family PKSN32 was not linked to any of the known recessive or dominant deafness loci (Hereditary Hearing Loss Homepage, http://dnalab-www.uia.ac.be/dnalab/hhh/ ). (bmj.com)
Identification1
Blast2
  • These are compiled into the set of sequences on the blast server. (uthsc.edu)
  • If you blast the sequence found in the Flybase record against the known or hypothesized full length cDNA of your gene (can also be found through FlyBase , then you'll know if your clone is full-length or will at least have an idea how much of the cDNA you have. (indiana.edu)
Publicly1
  • In 2013, the genome sequences of O. ulmi and O. novo-ulmi were publicly released. (sisef.it)
Functional genomics1
  • These sequence datum have a significant impact to the research community studying fungal biology, field ecology, structural genomics, comparative genomics, and functional genomics. (usda.gov)
Generate1
  • In order to reduce and eliminate aflatoxin contamination of food and feed, a project to generate Aspergilllus flavus Expressed Sequence Tags (ESTs) have been initiated. (usda.gov)
Technology2
  • The technology is based on 'sequencing-by-synthesis' technology, which begins by breaking up the DNA sample into fragments that are between 300 and 500 bases in length. (outsourcing-pharma.com)
  • Kartra is an all-in-one platform that includes internet sites, touchdown pages, email marketing/automation with tags and sequences, cart abandonment modern technology, digital downloads for membership websites, native helpdesk support with product wiki capabilities along with total CRM capability. (desiluweb.com)
Translation9
  • It makes your site translation ready and cross-browser compatible. (themeforest.net)
  • Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. (ucsf.edu)
  • Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags. (ucsf.edu)
  • Click a Field or Translation tag to filter the publications. (ucsf.edu)
  • Results: A dataset of 100 EST sequences, 50 with and 50 without, translation initiation sites, was created. (umn.edu)
  • Based on analysis of this dataset, ATGpr is found to be the most accurate for predicting the presence versus absence of translation initiation sites. (umn.edu)
  • As a baseline for comparison, choosing the first ATG correctly identifies the translation initiation site in 74% of the sequences. (umn.edu)
  • ESTScan and Diogenes, consistent with their intended use, are able to identify open reading frames, but are unable to determine the precise position of translation initiation sites. (umn.edu)
  • Using this system we are able to predict the exon-intron structure of a given gene with known translation initiation and stop co don site. (tu-berlin.de)
Markers2
  • We aimed to identify resistance gene analogs (RGAs) from peanut expressed sequence tags (ESTs) and to develop polymorphic markers. (jipb.net)
  • Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers [J]. J Integr Plant Biol. (jipb.net)
Complete1
  • Several sequences may join together to form complete sequences. (uthsc.edu)
High1
  • The high-quality physical map constructed in this study paves the way for the assembly of a reference sequence, from which breeding efforts will greatly benefit. (biomedcentral.com)
Integrate1
  • If you do decide you want to use Substack in conjunction with your Squarespace website, it's pretty easy to integrate the sign up form, and I can point you to my instructions that show how. (baipa.org)
Methods1
  • The indicators, strategies, setups, methods, and all other products and features on this website are for educational purposes only and should not be construed as advice. (ninjacators.pro)
Fully2
  • It was also the first fully sequenced genome of a multicellular organism to be completed. (wikipedia.org)
  • These homepage demos are fully equipped with pages and elements that a real estate listing website needs. (themeforest.net)
Content1
  • You can't tag subscribers, deliver tailored email content, send automated sequences, or upsell related books and services. (baipa.org)
Real estate3
  • Homlisti is the best Real Estate WordPress theme with a creatively crafted, clean, contemporary, and classy layout. (themeforest.net)
  • I would much prefer that you drive traffic to your own website, which is a piece of real estate you'll own forever. (baipa.org)
  • David is the epitome of a how a real estate agent should care for their clients. (metahers.xyz)
Source1
  • The flat file includes the sequence and the information of submitters, references, source organisms, and "feature" information, etc. (nig.ac.jp)
Project2
  • It stemmed from an Expressed Sequence Tag (EST) project that began at The Genome Institute at Washington University School of Medicine. (wikipedia.org)
  • The site was launched in 2000 to accompany the project "A Genomic Approach to Parasites from the Phylum Nematoda," funded by the National Institute of Allergy and Infectious Diseases (NIAID). (wikipedia.org)
Length1
  • presumably because the corresponding DNA sequences are so repetitious that they are hard to piece together into full-length sequences. (udel.edu)