• Adherence to intestinal cells is a key process in infection caused by enterohemorrhagic Escherichia coli (EHEC). (utmb.edu)
  • In this study, we sought to characterize the binding of EHEC O157:H7 to ECM proteins commonly present in the intestine. (utmb.edu)
  • Further characterization of this phenotype, by using antiserum raised against the LpfA1 putative major fimbrial subunit and by addition of mannose, showed that a reduced binding of EHEC to ECM proteins was observed in a long polar fimbria (lpf) mutant. (utmb.edu)
  • Extracellular matrix (ECM) proteins might act as receptors involved in the recognition of enteric pathogens, including EHEC. (utmb.edu)
  • Finally, increased bacterial adherence was observed when T84 cells, preincubated with ECM proteins, were infected with EHEC. (utmb.edu)
  • Taken together, these findings suggest that the interaction of Lpf and ECM proteins contributes to the EHEC colonization of the gastrointestinal tract. (utmb.edu)
  • Abstract: '[Investigators] carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli. (harvard.edu)
  • article{90864db6-10df-4743-8950-6627e8f974b9, abstract = {The complex I subunits NuoL, NuoM and NuoN are homologous to two proteins, MrpA and MrpD, from one particular class of Na+/H+ antiporters. (lu.se)
  • Mutant FtsZ84 protein polymerized inefficiently, suggesting that polymerization is important for the cellular role of FtsZ in division. (pnas.org)
  • Depletion of YhcB, an inner membrane protein of Escherichia coli , inhibited the growth of rodZ deletion mutant showing that the loss of both YhcB and RodZ is synthetically lethal. (hindawi.com)
  • isolated another E. coli mutant strain, strain LH530, that was hypersensitive to hydrophobic and large hydrophilic antibiotics and showed temperature-sensitive growth. (genetics.org)
  • It was found that the acetylase activity specific for protein L12 was negligible, when assayed in vitro, in the high-speed supernatant prepared from mutant cells. (springer.com)
  • I. A mutant lacking the N-terminal acetylation of protein S5 exhibits thermosensitivity. (springer.com)
  • Studies of a mutant lacking the N-terminal acetylation of protein S18. (springer.com)
  • To investigate the role of EspA and EspB in EPEC pathogenesis, we constructed mutant strains in E. coli serotype O103. (rupress.org)
  • Induction of protein production at the onset of cultivation decreased growth rate and glucose consumption rate for both the WT and the mutant strains. (uva.nl)
  • This mutant HypB fully supports the production of [NiFe]-hydrogenase in E. coli . (rsc.org)
  • The versatile Escherichia coli facilitates protein expression with relative simplicity, high cell density on inexpensive substrates, well known genetics, variety of expression vectors, mutant strains, co-overexpression technology, extracytoplasmic secretion systems, and recombinant protein fusion partners. (uwaterloo.ca)
  • The poor expression in the cytoplasm was improved by several strategies, including coexpression of the cytoplasmic chaperone GroEL/ES, using a mutant E. coli host strain with an oxidative cytoplasm, and protein fusion technology. (uwaterloo.ca)
  • When added to the culture medium, the recombinant carbohydrate-free GM2-activator, carrying the hexahistidine tail, could be taken up efficiently and restored the degradation of ganglioside GM2 to normal rates in mutant fibroblasts with the AB variant of GM2-gangliosidosis, which is characterized by a genetic defect in the GM2-activator protein. (biochemj.org)
  • In addition, RSE adherence of a DMEM-grown-O157 mutant lacking the intimin protein was comparable to that seen with its wild-type parent O157 strain grown in the same media. (harvard.edu)
  • We cloned a gene for a putative norfloxacin efflux protein from the chromosomal DNA of V. parahaemolyticus by using an Escherichia coli mutant lacking the major multidrug efflux system AcrAB as the host and sequenced the gene ( norM ). (asm.org)
  • An immunosorbent method using antiglutaredoxin-Sepharose was developed for purification of glutaredoxin in high yield from a mutant strain of Escherichia coli K 12 lacking thioredoxin reductase (C 10-17). (eurekamag.com)
  • The secreted EHEC proteins are recognized by rabbit antiserum raised against the proteins secreted from EPEC and by human serum from a patient infected with an EHEC O157:H7 strain. (asm.org)
  • Comparative proteomic analysis of differentially expressed proteins in the earthworm Eisenia fetida during Escherichia coli O157:H7 stress. (nextbio.com)
  • We suggest that the variable levels and trends in these spots on the gel may be useful as biomarker profiles to investigate E. coli O157:H7 contamination levels in soils. (nextbio.com)
  • However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins can be prepared. (mdpi.com)
  • Using a combination of LC-MS/MS approaches with protein and peptide fractionation steps we identified 1103 proteins from the cytosolic fraction of the Escherichia coli strain MC4100. (biomedcentral.com)
  • While both methods provided high-quality abundance data for nearly the entire proteome, their dependence on the availability of a strain library containing tagged versions of all proteins of interest presents a serious limitation. (biomedcentral.com)
  • To investigate the role of Esp proteins in disease, mutations in espA and espB were constructed in rabbit EPEC serotype O103 and infection characteristics were compared to that of the wild-type strain using histology, scanning and transmission electron microscopy, and confocal laser scanning microscopy in a weaned rabbit infection model. (rupress.org)
  • In order to increase yield of the recombinant protein, several strategies such as codon optimization, signal sequence tagging and bacterial strain engineering have been developed. (icheme.org)
  • The deletion strains could be complemented in trans by their respective Mrp protein, but expression of MrpA in the B. subtilis Delta mrpD strain and vice versa did not improve growth at pH 7.4. (lu.se)
  • Metagenomic studies have identified specific bacterial species and strains with increased prevalence in CD patients, amongst which is the adherent-invasive Escherichia coli (AIEC) strain LF82. (ugent.be)
  • A new colicin that adsorbs to the outer membrane protein Tsx but is dependent on the tonB instead of the tolQ membrane transport system. (springer.com)
  • Consistent with a coupling between translocation across the SecYEG translocon and folding by periplasmic chaperones, a lack of PpiD retards the release of a translocating outer membrane protein into the periplasm. (labome.org)
  • The C-terminal signal was successfully fused to a hybrid protein containing a few residues of ss-galactosidase and the majority of E. coli outer membrane protein OmpF lacking its own NH2-terminal signal sequence. (le.ac.uk)
  • Transporters of the RND family consist of several subunits (usually three), and an outer membrane protein(s) is involved in the drug transport. (asm.org)
  • Energy-coupled transport and signal transduction through the gram-negative outer membrane via the TonB-ExbB-ExbD dependent receptor proteins. (springer.com)
  • Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli. (biomedsearch.com)
  • Diffraction-quality crystals have been obtained of the integral membrane protein ferric enterobactin receptor (FepA) from the outer membrane of Escherichia coli. (biomedsearch.com)
  • This cooperativity among effector proteins explains how bacterial pathogens are able to effectively suppress innate immune defense mechanisms in response to diverse classes of immune receptor signaling complexes (RSCs) stimulated during infection. (asm.org)
  • We have devised a generally applicable strategy for analysis of protein structure and have applied it to examine the structure of the transmembrane portion of the Tar receptor of Escherichia coli. (caltech.edu)
  • Using a yeast two-hybrid screen, we identified Tmp21, a type-I integral membrane protein and COPI-vesicle receptor involved in trans-Golgi network function, as an NleF-binding partner. (k-state.edu)
  • Surface topology and sequence conservation in the adhesin domain hint at a putative receptor-binding pocket as found in the Klebsiella pneumoniae MrkD and E. coli F17-G (GafD) adhesins. (ugent.be)
  • LpfD binding was found to be resistant to treatment with O- or N-glycosidases, but was lost in collagenase-treated tissue sections, indicating the possible involvement of an intestinal matrix-associated protein as the LpfD receptor. (ugent.be)
  • Protein 1 was shown to be the receptor for phage PA-2 by the observations that the purified protein inactivates the phage, mutants lacking the protein are resistant to the phage, and mutants selected for PA-2 resistance have altered protein. (semanticscholar.org)
  • The 22 parameters used in the final model based on proteins' amino acid composition are discussed. (springer.com)
  • Hopp TP, Woods KR (1981) Prediction of protein antigenic determinants from amino acid sequences. (springer.com)
  • Heiland I, Wittmann-Liebold B (1979) Amino acid sequence of the ribosomal protein L21 of Escherichia coli . (springer.com)
  • The tool PERISCOPE is freely available for the researcher to predict the level of protein expressed based on the combination of signal sequence and amino acid sequence of a protein. (icheme.org)
  • To confirm the fidelity of the expression, the N-terminal ten amino acids of the recombinant rat kallikrein-binding protein were sequenced and were shown to match perfectly with those of the native rat kallikrein-binding protein. (biochemj.org)
  • Two mixtures of amino acids were fed together with glucose in fed-batch cultures aiming to improve a biomass yield and a concentration of the ZZT2 protein. (openthesis.org)
  • The other mixture contained only five socalled "protein amino acids", which were reported to be primarily incorporated into biomass: alanine, arginine, methionine, histidine and phenylalanine. (openthesis.org)
  • The mixture of five "protein amino acids" in shake flask culture even inhibited the cellular growth, which is seen as an indicator of disturbed metabolism caused by added amino acids. (openthesis.org)
  • Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. (uwaterloo.ca)
  • The H6-ZZ tag was cleaved off with Tobacco Etch Virus (TEV) protease and the 166 amino acid full-length homo-dimeric protein was purified using affinity and size-exclusion chromatography. (diva-portal.org)
  • In certain proteins or groups of proteins, the so-called spikes of high content for a specific amino acid type or combination of amino acids were identified and confirmed statistically, which in some cases could be directly related to function and ligand specificity. (figshare.com)
  • Locations of specific amino acid types in some of the proteins that have crystal structures (EmrE, LacY, AcrB) were also considered to help link amino acid content with protein function. (figshare.com)
  • Small angle X-ray scattering experiments indicate that the L-arabinose-binding protein undergoes a substantial conformational change upon the binding of substrate: the radius of gyration of the protein molecule decreases by (TURN)1 A with the addition of L-arabinose. (rice.edu)
  • Depletion of Ffh, a core component of the signal recognition particle of E.coli , appears to have a slight effect on the export of the TMAO reductase. (embopress.org)
  • A wide variety of proteins which are synthesised in the cytoplasm of E. coli are subsequently directed either to non-cytoplasmic compartments or transported to the extracellular medium. (le.ac.uk)
  • The primary structure of the protein was determined by analyses of [14C]carboxymethylated glutaredoxin and its proteolytic fragments obtained by digestions with trypsin, clostripain, chymotrypsin and staphylococcal Glu-specific extracellular protease. (eurekamag.com)
  • For example, the chemotaxis signal transducer CheY (14 kDa) was tagged with yellow fluorescent protein (YFP), producing a fusion protein of about 41 kDa ( 3 , 22 ) It remains an open question how much the addition of a substantial fluorescent tag might perturb the mobility of the protein of interest. (asm.org)
  • The fusion protein production was induced by isopropyl-beta-D-thiogalactopyranoside and they were purified by Glutathione Sepharose 4B. (bio-protocol.org)
  • The presence of the Myc- but not of the His(6)-tag on the cell surface during H:gm expression allowed us to confirm the hypothesis that this fusion protein was present on the surface and oriented towards the cell exterior. (diva-portal.org)
  • Modeling and Simulation of Production of Metallothionein and Red Fluorescent Fusion Protein by Recombinant Escherichia Coli Using Graphical Programming, Modeling, Programming and Simulations Using LabVIEW™ Software Riccardo De Asmundis, IntechOpen, DOI: 10.5772/14091. (intechopen.com)
  • Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile. (deepdyve.com)
  • This paper reports the ability of transgenic Arabidopsis thaliana plants to produce a fusion protein consisting of the B subunit of the Escherichia coli heat-labile enterotoxin and a 6 kDa tuberculosis antigen, the early secretory antigenic target ESAT-6. (deepdyve.com)
  • Both components of the fusion protein were detected using GM1-ganglioside-dependent enzyme-linked immunosorbant assay. (deepdyve.com)
  • This suggested the fusion protein retained both its native antigenicity and the ability to form pentamers. (deepdyve.com)
  • Combined with previous observations that YbbN enhances the DnaK-DnaJ-GrpE chaperone system, we propose that YbbN coordinately regulates the activities of these two prokaryotic chaperones, thereby helping to direct client protein traffic initially to DnaK. (unl.edu)
  • To resolve these problems, we have carried out a systematic study of the size dependence of protein diffusion coefficients in the Escherichia coli cytoplasm, using engineered GFP multimers (from 2 to 6 covalently linked GFP molecules). (asm.org)
  • To resolve the question of the size dependence of protein diffusion in the E. coli cytoplasm, FRAP was used to measure diffusion coefficients ( D ) for a series of engineered GFP oligomers, ranging in size from 30 kDa (GFP monomers) to 165 kDa (six linked GFP molecules). (asm.org)
  • Many proteins contain a thioredoxin (Trx)-like domain fused with one or more partner domains that diversify protein function by the modular construction of new molecules. (unl.edu)
  • Thus, it is possible to detect a variety of disulfide cross-links between and within individual protein molecules. (caltech.edu)
  • Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cells. (springer.com)
  • The contribution of protein induction and repression to the adaptation of cells to changes in oxygen supply is only poorly understood. (asm.org)
  • We assessed this contribution by measuring the levels of 170 individual polypeptides produced by Escherichia coli K-12 in cells growing aerobically or anaerobically with and without nitrate. (asm.org)
  • Within bacterial cells such as Escherichia coli , similar measurements are challenging because of the small dimensions of the cell. (asm.org)
  • Nevertheless, studies of the mobility of fluorescently tagged proteins are starting to give powerful insights into the dynamics of processes occurring in living bacterial cells. (asm.org)
  • All of these studies depend on the use of cells engineered to express fusion proteins in which the protein of interest is fused to a fluorescent protein tag, usually a variant of green fluorescent protein (GFP). (asm.org)
  • Surface proteins of Escherichia coli K12 were identified by radiolabelling using 1,3,4,6 - tatrachloro, 3-alpha, 6-alpha - diphenylgycoluryl (Iodo-Gen) and /sup 131/I. Labelled proteins were localized in the outer membrane of the cells. (osti.gov)
  • Radiolabelling of E.coli cells inculbated at 42/sup 0/C showed that the syntheses of two surface proteins were temperature-inducible. (osti.gov)
  • Proteins fulfill a wide variety of functions and are central to almost all processes in living cells. (biomedcentral.com)
  • β-Glucuronidase from Escherichia coli has been used in the enzymatic cleavage and activation of glucuronide prodrugs in non-small cell lung cancer cells, U87 human glioblastoma cell line, in A549 (human lung adenocarcinoma) and KB (human oral squamous carcinoma). (sigmaaldrich.com)
  • These secreted proteins are essential for triggering of host signal transduction pathways in tissue culture cells. (rupress.org)
  • Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antibodies-online.com)
  • Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. (harvard.edu)
  • tools in the context of an environmental perturbation of Escherichia coli cells-the addn. (epfl.ch)
  • The authors also tested the application of these tools to the study of a genetic perturbation of Escherichia coli cells-the ability of certain strains to hypersecrete the hemolysin protein. (epfl.ch)
  • Background: In this study, we present evidence that proteins encoded by the Locus of Enterocyte Effacement (LEE), considered critical for Escherichia coli O157 (O157) adherence to follicle-associated epithelial (FAE) cells at the bovine recto-anal junction (RAJ), do not appear to contribute to O157 adherence to squamous epithelial (RSE) cells also constituting this primary site of O157 colonization in cattle. (harvard.edu)
  • Results: Antisera targeting intimin-γ, the primary O157 adhesin, and other essential LEE proteins failed to block O157 adherence to RSE cells, when this pathogen was grown in DMEM, a culture medium that enhances expression of LEE proteins. (harvard.edu)
  • This suggested that proteins other than intimin-γ that contribute to adherence to RSE cells are expressed by this pathogen during growth in DMEM. (harvard.edu)
  • Conclusion: Proteins other than LEE and intimin-γ proteins are involved in O157 adherence to RSE cells at the bovine RAJ. (harvard.edu)
  • Cells of E. coli transformed with the cloned ydhE gene showed elevated resistance to norfloxacin, ciprofloxacin, acriflavine, and tetraphenylphosphonium ion, but not to ethidium, when MICs were measured. (asm.org)
  • Escherichia coli HypB binds nickel with sub-picomolar affinity, and the formation of the HypB-SlyD complex activates nickel release from the high-affinity site (HAS) of HypB. (rsc.org)
  • cspA protein binds Hfq, which upregulates production of sigma factors which mediate a stress response. (wikipedia.org)
  • BACKGROUND: The goal of many proteomics experiments is to determine the abundance of proteins in biological samples, and the variation thereof in various physiological conditions. (duke.edu)
  • During my Ph.D. I have tried to understand the reasons for this toxicity by studying the consequences of membrane protein over-expression using a combination of proteomics and more focused biochemical and genetic methods. (avhandlingar.se)
  • Several methods for labeling proteins metabolically (in cell cultures) or after extraction are widely applied in "shotgun" proteomics. (biomedcentral.com)
  • One of the few organisms where direct protein concentrations are available on a nearly proteome wide level is the yeast Saccharomyces cerevisiae . (biomedcentral.com)
  • A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antibodies-online.com)
  • In total the protein was deduced to have 85 residues corresponding to a molecular weight of 9674 for the reduced form of glutaredoxin, making it one of the smallest known enzymes (a glutathione-disulfide transhydrogenase). (eurekamag.com)
  • They also confirm that glutaredoxin lacks the disulfide-adjacent tryptophan residues of E. coli thioredoxin. (eurekamag.com)
  • Protein hydroxylation at proline and lysine residues is known to have important effects on cellular functions, such as the response to hypoxia. (luriechildrens.org)
  • However, protein hydroxylation at tyrosine residues (called protein-bound 3,4-dihydroxy-phenylalanine (PB-DOPA)) has not been carefully examined. (luriechildrens.org)
  • The recently solved X-ray crystal structures of three class C LMM-PBPs (E. coli PBP4, Bacillus subtilis PBP4a, and Actinomadura R39 DD-peptidase) have identified several residues that form a pocket in the active site unique to this class of PBP. (warwick.ac.uk)
  • Equivalent residues of E. coli PBP4 (Asp 155, Phe160, Arg361, and Gln422) were mutated, and the effect on both DD-carboxypeptidase and DD-endopeptidase activities was determined. (warwick.ac.uk)
  • When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 degrees C or 15 degrees C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. (pnas.org)
  • The movement of proteins within the cytoplasm must be constrained by a combination of viscosity, macromolecular crowding, and specific interactions of the protein with other cell components (e.g., other proteins, nucleic acids, and the cytoplasmic membrane). (asm.org)
  • However, both Sec‐dependent and Sec‐independent protein insertion into the E.coli cytoplasmic membrane have been reported. (embopress.org)