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  • strain
  • A rarer and more virulent Shiga-toxin-producing strain, E. coli O104:H4, was the cause of the 2011 outbreak centered on N Germany that sickened more than 4,000 people from more than a dozen countries and killed 50. (thefreedictionary.com)
  • bacteria
  • YjeE represents a protein family whose members are broadly conserved in bacteria, absent from eukaryotes and contain both Walker A and B motifs, characteristic of P-loop ATPases. (biochemj.org)
  • Colicins are toxic exoproteins produced by bacteria of colicinogenic strains of Escherichia coli and some related species of Enterobacteriaceae , during the growth of their cultures. (springer.com)
  • receptor
  • Using a yeast two-hybrid screen, we identified Tmp21, a type-I integral membrane protein and COPI-vesicle receptor involved in trans-Golgi network function, as an NleF-binding partner. (k-state.edu)
  • Synthesis
  • The rate of CS7.4 production reached 13% of total protein synthesis within 1-1.5 hr after a shift to 10 degrees C and subsequently dropped to a lower basal level. (pnas.org)
  • The higher proteolysis rate and declining synthesis rate are thought to be the reasons for the lower yields of the protein ZZT2 in the large-scale and scale-down cultivations. (openthesis.org)
  • survival
  • The human pathogens enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC), as well as the mouse pathogen Citrobacter rodentium encode type III secretion system (T3SS) effector proteins to promote their survival in the infected host. (k-state.edu)
  • describes
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • concentration
  • The production of the recombinant protein ZZT2 was highest in the lab-scale cultivation (50 mg/g), however in the large-scale the ZZT2 concentration declined 8 hours after induction from its highest value of 45 to a final value of 35 mg/g. (openthesis.org)
  • Cell
  • Colicin A unfolds during its translocation in Escherichia coli cells and spans the whole cell envelope when its pore has formed. (springer.com)
  • When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 degrees C or 15 degrees C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. (pnas.org)
  • The molecule produced by Escherichia coli is slightly larger than the M protein isolated by solubilization of the streptococcal cell wall, but is similar in size to that secreted by streptococcal protoplast and L forms. (sciencemag.org)
  • sequence
  • View conserved domains detected in this protein sequence using CD-search. (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • production
  • 2016). Here, detailed conditions are presented which allow the production of GST-Cbx7 fusion proteins from E. coli . (bio-protocol.org)
  • The effects of scale-up on the production of the ZZT2 protein were investigated in reactors of different configuration and scale: a well-mixed lab-scale bioreactor, an industrial scale 12-m3 bioreactor, and a two-compartment scale-down bioreactor. (openthesis.org)
  • variants
  • We have looked critically for ATPase activity in the recombinant E. coli protein and have made vigilant use of site-directed variants in the Walker A [K41A (Lys 41 →Ala) and T42A] and putative Walker B (D80Q) motifs. (biochemj.org)
  • We noted that any hydrolysis of ATP by the wild-type E. coli protein might be attributed to background ATPase, since it was not appreciably different from that of the variants. (biochemj.org)
  • website
  • The way to use this model on the website http://www.ou.edu/ for the prediction of protein solubility is explained. (springer.com)
  • systems
  • The quantification of protein-ligand interactions is essential for systems biology, drug discovery, and bioengineering. (duke.edu)
  • various
  • The purification of fusion proteins by GST fusion system has been widely applied in various biochemical and structural studies (Harper and Speicher, 2011). (bio-protocol.org)
  • accuracy
  • be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein. (uniprot.org)
  • list
  • section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein. (uniprot.org)
  • analysis
  • Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes. (duke.edu)