• Serum of cases and controls obtained at recruitment were analysed for ASCA IgG, ASCA IgA, perinuclear anti-neutrophil cytoplasmic antibody (pANCA), antibodies against Escherichia coli outer membrane porin C (OmpC) and flagellin CBir1. (lu.se)
  • Disruption of polyamine modulation by a single amino acid substitution on the L3 loop of the OmpC porin channel. (tamu.edu)
  • Here we report the effects of the mutation of two different aspartate residues of Escherichia coli OmpC porin on the polyamine sensitivity of the channel. (tamu.edu)
  • The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro. (unab.cl)
  • Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC. (unab.cl)
  • Since alcohol primarily targets the integrity of the membrane, maintenance of outer membrane OmpC levels in the presence of alcohol might help in the survival of E. coli to higher alcohol concentrations. (biomedcentral.com)
  • Our work deals primarily with the outer membrane proteins, lambda receptor, the product of the lamB gene, and the major outer membrane (porin) proteins 1a and 1b, products of the ompF and ompC genes. (unibas.ch)
  • The high-affinity interaction of LF with pore-forming outer-membrane proteins (OMPs) of Gram-negative enterics, including Escherichia coli , is critical for the antimicrobial outcome of LF (Gado et al. (ift.org)
  • Use of a Siderophore Receptor and Porin Proteins-Based Vaccine to Control the Burden of Escherichia coli O157:H7 in Feedlot Cattle. (foodengineeringmag.com)
  • Mutations that result in alterations of the outer membrane porin proteins of Gram-negative organisms lead to decreased permeability of the drug through the outer membrane so less drug reaches the target enzyme. (cdc.gov)
  • The fluoroquinolone susceptibility profile for each clinical isolate is determined by the number and location of mutational changes in specific enzyme target sites, porin proteins, and efflux mechanisms. (cdc.gov)
  • To investigate the mode of action of these proteins, we purified recombinant FupA, FupB and FupA/B proteins expressed in Escherichia coli and incorporated them into mixed lipid bilayers. (encyclopedia.pub)
  • The monomeric porin OmpG is especially attractive compared with multisubunit proteins because appropriate modifications of the pore can be easily achieved by mutagenesis. (ox.ac.uk)
  • We are studying the process by which proteins are exported to the envelope region of Escherichia coli. (unibas.ch)
  • The structural genes for the porin proteins, 1a and 1b, are regulated at the transcriptional level by the ompB locus. (unibas.ch)
  • With this genetic system, it should be possible to determine if E coli employs more than a single mechanism for the export of proteins to the outer membrane. (unibas.ch)
  • Carbapenem-resistant Escherichia coli clinical isolates from a tertiary hospital in Wenzhou, east China, were investigated for NDM-1 production. (biomedcentral.com)
  • The present study is the first report of bla NDM-1 carriage in E. coli ST167 isolates and coexistence of bla NDM-1 and bla CMY-42 in same isolate. (biomedcentral.com)
  • In the present study, we identified bla NDM-1 among two clonally related E. coli isolates belonging to ST167 from one tertiary hospital in Wenzhou, east China, among which bla NDM-1 was found to coexist with bla CTX-M-14 and bla CMY-42 . (biomedcentral.com)
  • Escherichia coli and Klebsiella pneumoniae isolates were obtained from the Canadian Ward Surveillance Study (CANWARD) and underwent in vitro susceptibility testing to determine prevalence and antimicrobial resistance patterns. (umanitoba.ca)
  • Porin alteration was found to be a factor leading to carbapenem reduced susceptibility among isolates. (umanitoba.ca)
  • Isolates of E.coli (n=42) and K. pneumoniae (n=134) from blood culture collected during 2013-2015 were screened for carbapenemase production by using carba NP test and the presence of carbapenem resistant genes (KPC, IMP, VIM, NDM and OXA- 48 like). (omicsonline.org)
  • During the same period, almost half of Escherichia coli isolates developed resistance to cefotoxime. (who.int)
  • Functional characterization of KPC-109 was performed investigating the impact of KPC-109 production on the β-lactam resistance phenotype of various Escherichia coli and Klebsiella pneumoniae strains, including derivatives of K. pneumoniae with OmpK35 and OmpK36 porin alterations. (imperial.ac.uk)
  • In Klebsiella pneumoniae (KP), modifications in the porin OmpK36 are implicated in increasing resistance to carbapenems. (imperial.ac.uk)
  • Enterobacteriaceae , particularly Escherichia coli and Klebsiella pneumioniae , are common pathogens causing nosocomial infections. (biomedcentral.com)
  • High-level carbapenem-resistant OXA-48-producing Klebsiella pneumoniae with a novel OmpK36 variant and low-level, carbapenem-resistant, non-porin-deficient, OXA-181-producing Escherichia coli from Thailand. (kku.ac.th)
  • Resistance to quinolones occurs through chromosomal mutations in the genes encoding these enzymes and by porin and efflux mutations. (cdc.gov)
  • Detection of CTX-M-27 β-lactamase genes on two distinct plasmid types in ST38 Escherichia coli from three US states. (cdc.gov)
  • Escherichia coli rapidly develops resistance, particularly that associated with multiple drug resistance (MDR) when exposed to selected antimicrobials. (vin.com)
  • In human medicine, E. coli has developed resistance to the fluorinated quinolones, beta-lactams, or both: it is among the gram-negative organisms that secrete extended-spectrum beta-lactamases (ESBL). (vin.com)
  • Effects of Halides on Plasmid-Mediated Silver Resistance in Escherichia coli. (biophysica.com)
  • However, in the presence of porin alterations commonly encountered in high-risk clonal lineages of K. pneumoniae, KPC-109 was able to confer clinical-level resistance to carbapenems as well. (imperial.ac.uk)
  • E. coli WZ33 and WZ51 exhibited discrepant-level resistance to most clinically frequent used antimicrobials, but still susceptible to trimethoprim/sulfamethoxazole, amikacin, fosfomycin, tigecycline and polymyxin B. E. coli WZ33 and WZ51 were positive for bla NDM-1 determined by PCR and DNA sequencing. (biomedcentral.com)
  • Carbapenem resistance for E. coli WZ51 and WZ33 could not be transferred to E. coli recipients through conjugation, but could be transferred to E. coli recipients by chemical transformation. (biomedcentral.com)
  • Many resistant organisms have multiple enzyme target site, porin, and efflux mutations, producing high-level resistance to quinolones. (cdc.gov)
  • Several studies have been conducted to examine the role of outer membrane modifications such as porins, lipopolysaccharides, and efflux pumps on antibiotic resistance. (uky.edu)
  • Escherichia coli , Staphylococcus aureus or Mycobacteria tuberculosis are due to failure in therapy caused by resistance to the chemotherapeutic agents. (openmicrobiologyjournal.com)
  • Mutations associated with dysfunction of the porin OprD were found in 888 (87.1%) of the genomes and were correlated with carbapenem resistance, and a machine learning model incorporating hundreds of genetic variations among the chromosomal mechanisms of resistance was able to classify resistant genomes. (cdc.gov)
  • Further, it is highly mutagenic, with spontaneous mutations occurring in 1 per 100 thousand to 1 per billion new progeny (assume 1 g of feces contains 100 million E. coli ), thus ensuring opportunity for spontaneous mutation even in the absence of stimuli, such as drugs. (vin.com)
  • The enzyme target site, porin, and efflux mutations may result from the selective pressure of exposure of the organism to antimicrobial agents during therapy and may cause treatment failure. (cdc.gov)
  • The OmpG porin with both stabilizing mutations exhibited a 95% reduction in gating activity. (ox.ac.uk)
  • Only 1 of each KPC-producing E. coli and K. pneumoniae was found. (umanitoba.ca)
  • Genetic relatedness of CRS/CIR E. coli and K. pneumoniae was determined using pulsed-field gel electrophoresis. (umanitoba.ca)
  • Infections caused by extended-spectrum -lactamase (ESBL)-producing Escherichia coli are a significant cause of morbidity and healthcare costs. (cdc.gov)
  • Pfizer Animal Health recently acquired global rights to distribute an E. coli bacterial extract vaccine with SRP technology from Epitopix LLC. (foodengineeringmag.com)
  • In this regard, Escherichia coli is of special interest, since it is genetically tractable and the most well-understood bacterial species that is widely considered for commercial biofuel production [ 6 , 7 ]. (biomedcentral.com)
  • Bacterial outer membrane porins have a robust beta-barrel structure and therefore show potential for use as stochastic sensors based on single-molecule detection. (ox.ac.uk)
  • Enterotoxigenic Escherichia coli (ETEC) causes diarrheal disease. (biomedcentral.com)
  • Ozaki H, Sato T, Kubota H, Hata Y, Katsube Y, Shimonishi Y (1991) Molecular structure of the toxic domain of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli . (springer.com)
  • Hidaka Y, Kubota H, Yoshimura S, Ito H, Takeda Y, Shimonishi Y (1988) Disulfide linkages in heat-stable enterotoxin (STp) produced by a porcine strain of enterotoxigenic Escherichia coli . (springer.com)
  • Yoshimura S, Ikemura H, Watanabe H, Aimoto S, Shimonishi Y, Hara S, Takeda T, Miwatani T, Takeda Y (1985) Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli . (springer.com)
  • La sensibilité de Staphylococcus aureus à l'oxacilline a diminué significativement, passant de 95,0 % entre 1999 et 2002 à 84,4 % en 2008. (who.int)
  • 1983). Outer membrane protein K of Escherichia coli: purification and pore-forming properties in lipid bilayer membranes. (wikipedia.org)
  • I studied the distribution of fluoroquinolones in the two aqueous compartments (periplasm and cytoplasm) of Escherichia coli using fluorescence intensity measurement and minimum inhibitory concentration (MIC) test. (uky.edu)
  • We describe the global molecular epidemiology of 229 tional distribution of carbapenemase-producing E. carbapenemase-producing Escherichia coli in 36 coun- tries during 2015-2017. (cdc.gov)
  • We used this mutant for the detection of adenosine diphosphate at the single-molecule level, after equipping the porin with a cyclodextrin molecular adapter, thereby demonstrating its potential for use in stochastic sensing applications. (ox.ac.uk)
  • In contrast, organisms with decreased susceptibility produced only by porin changes usually have lower minimum inhibitory concentrations (MICs). (cdc.gov)
  • When vaccinated with the SRP, the cow's immune system is stimulated to make antibodies against the siderophore receptors and porins. (foodengineeringmag.com)
  • When exposed to the E. coli bacteria, the antibodies bind to the siderophore receptors and porins and help prevent iron from entering the E. coli cell, causing it to die. (foodengineeringmag.com)
  • Escherichia coli , a member of the family Enterobacteriaceae, is a lactose fermenter, causing a distinct color on diagnostic agar. (vin.com)
  • Systemic surveillance should focus on the dissemination of bla NDM-1 among Enterobacteriaceae, especially E. coli ST167 clone associated with animal infection. (biomedcentral.com)
  • The addition of Protein K in the outer membrane proven to cause an increased rate of uptake of nutrients and a faster growth rate relative to the parental porin- strain. (wikipedia.org)
  • We also created an E. coli strain that produced neutral OPGs. (uky.edu)
  • Takao T, Hitouji T, Aimoto S, Shimonishi Y, Hara S, Takeda T, Takeda Y, Miwatani T (1983) Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigentic Escherichia coli strain 18D. (springer.com)
  • For the treatment of shigellosis or GI infections caused by E. coli, some clinicians recommend that norfloxacin be given in a dosage of 400 mg twice daily for 3 days. (antiinfectivemeds.com)
  • Field M, Graf LH Jr, Laird WJ, Smith PL (1978) Heat-stable enterotoxin of Escherichia coli: In vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine. (springer.com)
  • Porin channels with a D118Q mutation, at the root of L3, still showed some, albeit milder, sensitivity to polyamine modulation. (tamu.edu)
  • Yamasaki S, Sato T, Hidaka Y, Ozaki H, Ito H, Hirayama T, Takeda Y, Sugimura T, Tai A, Shimonishi Y (1990) Structure-activity relationship of Escherichia coli heat-stable enterotoxin: Role of Ala residue at position 14 in toxin-receptor interaction. (springer.com)
  • Bottom): Those animals still testing positive showed a 98 percent reduction in concentrations of E. coli O157. (foodengineeringmag.com)
  • damage of DNA The NER system has been extensively studied in E. coli where the S63845 research buy uvr gene products protect bacteria from different types of DNA damages including those induced by UV radiations [14]. (faksignaling.com)
  • SRP, an acronym for siderophore receptors and porins, is a technology that inhibits E. coli's ability to seek and bind iron, which it needs to sustain growth. (foodengineeringmag.com)
  • Structural studies have demonstrated that the extracellular L3 loop of porin constricts the channel and suggest that this loop might be involved in channel selectivity and gating. (tamu.edu)
  • Summary: Colicins are a diverse family of large antibacterial protein toxins, secreted by and active against Escherichia coli and must cross their target cell's outer membrane barrier to kill. (nottingham.edu.cn)
  • Ikemura H, Takagi H, Inouye M (1987) Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli . (springer.com)
  • We examined the porin-forming activity of the FupA/B proteoliposomes using a fluorescent 8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt (ANTS) probe. (encyclopedia.pub)
  • When asked whether this seems expensive, he suggested that all the equipment added to production lines to fix the E. coli problem is not exactly cheap, probably hitting close to a million dollars or more per line. (foodengineeringmag.com)
  • The study provides important information about the membrane protein responses of E. coli to a range of alcohols, which can be used to develop targeted strategies for increased microbial alcohol tolerance and hence bioalcohol production. (biomedcentral.com)
  • The production of ethanol by E. coli is preferable as it can metabolize a range of substrates, including the sugars derived from lignocellulosic biomasses readily available from agricultural wastes [ 20 ]. (biomedcentral.com)
  • Among the most adaptable organisms is E. coli . (vin.com)
  • Organisms may have alterations in more than one enzyme target site and, in Gram-negative organisms, may contain more than one porin change. (cdc.gov)
  • Gupta DD, Saha S, Chakrabarti MK (2005) Involvement of protein kinase C in the mechanism of action of Escherichia coli heat-stable enterotoxin (STa) in a human colonic carcinoma cell line, COLO-205. (springer.com)
  • Although E. coli O157:H7 doesn't make cattle sick, exposure to the bacteria shed from cattle can make humans very sick. (foodengineeringmag.com)