Escherichia coli porin regulation. Medical search. Frequent questions

After serial passage, 8 of 10 strains developed cefoxitin resistance, none developed promoter or attenuator mutations, 6 lost both the OmpC and OmpF porin proteins, and 1 showed decreased production of both. (cdc.gov)
Porin mutants may occur more commonly and be less fit and less inclined to spread or cause disease than strains with increased β-lactamase expression. (cdc.gov)
Although cephamycin-resistant E. coli is relatively uncommon, widespread use of β-lactam antiboties may contribute to the development and spread of these strains. (cdc.gov)
At a local level, unpublished data from the Queen Elizabeth II Health Science Centre in Halifax, Nova Scotia indicated that, of the 5,767 strains of E. coli processed from urine samples, 0.4% were cephamycin resistant. (cdc.gov)
All strains of E. coli possess a gene that encodes an AmpC β - lactamase. (cdc.gov)
Surveys of resistance mechanisms in cephamycin-resistant strains have most often identified promoter or attenuator mutations, which results in an up-regulation of AmpC β-lactamase production ( 5 - 7 ). (cdc.gov)
We examined E. coli strains collected at our hospital to determine the basis for resistance. (cdc.gov)
In addition, we created cephamycin-resistant strains of E. coli by serial passage on cefoxitin-containing medium to determine which of these two resistance mechanisms was predominant and if our findings were representative of those seen in clinical isolates. (cdc.gov)
We collected strains of E. coli from midstream urine from inpatients and from patients in the community. (cdc.gov)
5. Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. (ac.ir)
We present our study on the efficacy and phenotypic impact of compensatory evolution in Escherichia coli strains carrying multiple resistance mutations. (elifesciences.org)
Cephalexin is particularly effective against gram bacteria, such as Staphylococcus aureus and Streptococcus pneumoniae but it also exhibits efficacy against certain gram-negative strains, like Escherichia coli (E.coli) and Klebsiella pneumonia, providing broad coverage against a range of bacterial infections. (buy-pharma.md)

Glucose is the essential carbon source for growing and cultivating heterotrophic bacteria, such as Escherichia coli , for laboratory and production purposes. (encyclopedia.pub)
While a change in diet for cows before slaughter is thought to cut E. coli O157 shedding, a vaccine may be a better method to stave off the bacteria. (foodengineeringmag.com)
Although E. coli O157:H7 doesn't make cattle sick, exposure to the bacteria shed from cattle can make humans very sick. (foodengineeringmag.com)
When exposed to the E. coli bacteria, the antibodies bind to the siderophore receptors and porins and help prevent iron from entering the E. coli cell, causing it to die. (foodengineeringmag.com)
Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
Curli, consisting primarily of major structural subunit CsgA, are functional amyloids produced on the surface of Escherichia coli, as well as many other enteric bacteria, and are involved in cell colonization and biofilm formation. (wustl.edu)
The tracking microscope was used to study the chemotactic responses of E. coli to temporal gradients of L-glutamate generated in isotropic solutions by the action of the enzyme alanine aminotransferase, finding that if the bacteria have a "memory," its decay time cannot be much longer than a few seconds. (typeset.io)
We explain the biology and physics underlying the chemotactic (foraging) behavior of E. coli bacteria. (typeset.io)
General porins are nature's sieving machinery in the outer membrane of Gram-negative bacteria. (ibecbarcelona.eu)

This Hfq-associated RNA is thought to be a regulator of the expression level of the OmpC porin protein, with a 5′ region of 22 nucleotides potentially forming an antisense interaction with the ompC mRNA. (wikipedia.org)
Since alcohol primarily targets the integrity of the membrane, maintenance of outer membrane OmpC levels in the presence of alcohol might help in the survival of E. coli to higher alcohol concentrations. (biomedcentral.com)
EnvZ is a histidine kinase that senses changes in medium osmolarity and signals its response regulator OmpR to bind to OmpF and OmpC porin promoters to differentially modulate their transcription. (mechanobio.info)

3. Khanfar HS, Bindayna KM, Senok AC, Botta GA. Extended spectrum beta-lactamases (ESBL) in Escherichia coli and Klebsiella pneumoniae: trends in the hospital and community settings. (ac.ir)
4. Kohlenberg A, Schwab F, Rüden H. Wide dissemination of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. (ac.ir)

To our knowledge, no investigators have concurrently looked for alterations in porins in addition to promoter-attenuator mutations. (cdc.gov)
In addition, diminished expression of outer membrane proteins, mutations in topoisomerases, and up-regulation of efflux pumps also play an important part in antibiotic resistance. (microbiologyjournal.org)

Enterotoxigenic Escherichia coli (ETEC) causes diarrheal disease. (biomedcentral.com)
Ozaki H, Sato T, Kubota H, Hata Y, Katsube Y, Shimonishi Y (1991) Molecular structure of the toxic domain of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli . (springer.com)
Hidaka Y, Kubota H, Yoshimura S, Ito H, Takeda Y, Shimonishi Y (1988) Disulfide linkages in heat-stable enterotoxin (STp) produced by a porcine strain of enterotoxigenic Escherichia coli . (springer.com)
Yoshimura S, Ikemura H, Watanabe H, Aimoto S, Shimonishi Y, Hara S, Takeda T, Miwatani T, Takeda Y (1985) Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli . (springer.com)

In addition, mutation or altered expression of outer membrane proteins constituting porins can also contribute to cephamycin resistance. (cdc.gov)
The high-affinity interaction of LF with pore-forming outer-membrane proteins (OMPs) of Gram-negative enterics, including Escherichia coli , is critical for the antimicrobial outcome of LF (Gado et al. (ift.org)

Escherichia coli is the best-known model for the biotechnological production of many biotechnological products, including housekeeping and heterologous primary and secondary metabolites and recombinant proteins, and is an efficient biofactory model to produce biofuels to nanomaterials. (encyclopedia.pub)
The genome of E. coli MG1655 is 4,641,642 bp, corresponding to 4702 genes encoding 4328 proteins. (encyclopedia.pub)
The genome size of E. coli strain K-12 MG1655 is 4,641,652 bp, corresponding to 4702 genes encoding 4328 proteins, 228 RNA genes, and 146 pseudogenes. (encyclopedia.pub)
Use of a Siderophore Receptor and Porin Proteins-Based Vaccine to Control the Burden of Escherichia coli O157:H7 in Feedlot Cattle. (foodengineeringmag.com)

In this regard, Escherichia coli is of special interest, since it is genetically tractable and the most well-understood bacterial species that is widely considered for commercial biofuel production [ 6 , 7 ]. (biomedcentral.com)
Pfizer Animal Health recently acquired global rights to distribute an E. coli bacterial extract vaccine with SRP technology from Epitopix LLC. (foodengineeringmag.com)
We explain a variety of bacterial swarming and social foraging behaviors and discuss the control system on the E. coli that dictates how foraging should proceed. (typeset.io)
That significant fractions of the bacterial genome could be involved in, or affected during, biofilm formation was shown in Escherichia coli in a genome-wide screen using random chromosomal insertions of a lacZ reporter gene fusion construct [ 5 ]. (biomedcentral.com)
A limited study had indicated that in Sinorhizobium meliloti the YbeY ortholog regulates the accumulation of sRNAs as well as the target mRNAs, raising the possibility that YbeY may play a previously unrecognized role in bacterial sRNA regulation. (biomedcentral.com)

Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa. (chrisdixie.com)

The MicC non-coding RNA (previously known as IS063 ) is located between the ompN and ydbK genes in E. coli. (wikipedia.org)
A study of Escherichia coli physiology during growth on glycerol was performed combining transcriptional-proteomic analysis as well as kinetic and stoichiometric evaluations in the strain JM101 and certain derivatives with important inactivated genes. (biomedcentral.com)
The expression of metabolic genes, in particular the overexpression of pykA pckA, gltA fumABC sdh mdh and acnA genes and the downregulation of the ackA gene, has been reported for E. coli growing on glycerol [ 7 ]. (biomedcentral.com)

AmpC β-lactamase, altered porins, or both are usually responsible for cefoxitin resistance in Escherichia coli . (cdc.gov)
Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin. (ac.ir)

The development of antibiotic resistance in Escherichia coli has important clinical implications. (cdc.gov)

In addition, porin alterations may protect E. coli and allow subsequent selection for promoter and attenuator mutants. (cdc.gov)

E. coli isolates were identified with conventional biochemical reactions. (cdc.gov)
isolates in Korea and their association with porin down-regulation. (ac.ir)

Although alcohol toxicity increased exponentially with alcohol chain length (2-6 carbon), similar stress responses were observed in the inner and outer membrane proteome of E. coli in the presence of 2-, 4- and 6-carbon alcohols at the MIC 50 . (biomedcentral.com)
The study provides important information about the membrane protein responses of E. coli to a range of alcohols, which can be used to develop targeted strategies for increased microbial alcohol tolerance and hence bioalcohol production. (biomedcentral.com)
We used the tracking microscope to study the chemotactic responses of E. coli to temporal gradients of L-glutamate generated in isotropic solutions by the action of the enzyme alanine aminotransferase. (typeset.io)
We have applied a multipronged approach of loss-of-function studies, genome-wide mRNA and sRNA expression profiling, pathway analysis, target prediction, literature mining and network analysis to unravel YbeY-dependent molecular responses of E. coli exposed to hydroxyurea (HU). (biomedcentral.com)

Yamasaki S, Sato T, Hidaka Y, Ozaki H, Ito H, Hirayama T, Takeda Y, Sugimura T, Tai A, Shimonishi Y (1990) Structure-activity relationship of Escherichia coli heat-stable enterotoxin: Role of Ala residue at position 14 in toxin-receptor interaction. (springer.com)

Porin alterations might work together to produce a higher level of resistance. (cdc.gov)
Escherichia coli , Staphylococcus aureus or Mycobacteria tuberculosis are due to failure in therapy caused by resistance to the chemotherapeutic agents. (openmicrobiologyjournal.com)

Mutagenesis of cysQ (Rv2131c) was initially unsuccessful, but was possible when a porin-like gene of Mycobacterium smegmatis was expressed, and also by gene switching in the merodiploid strain. (biomedcentral.com)

Nevertheless, due to the high number of sugar transporters, E. coli uses preferentially few systems to grow in glucose as the sole carbon source. (encyclopedia.pub)

Genome regulation encompasses all facets of gene expression, from the biochemical modifications of DNA, to the physical arrangement of chromosomes and the activity of the transcription machinery. (mechanobio.info)

Gupta DD, Saha S, Chakrabarti MK (2005) Involvement of protein kinase C in the mechanism of action of Escherichia coli heat-stable enterotoxin (STa) in a human colonic carcinoma cell line, COLO-205. (springer.com)
The mechanism of transport through porins is of utmost and direct interest for drug discovery, extending toward nanotechnology applications for blue energy, separations, and sequencing. (ibecbarcelona.eu)
The underlying mechanism of regulation of SPI-2 by SsrA/SSrB is the phopho-relay between the sensor kinase, SsrA, and the response regulator, SsrB. (mechanobio.info)

E. coli is among the most frequently isolated bacterium in a variety of clinical settings. (cdc.gov)

E. coli preferentially uses glucose in the presence of sugar mixtures, preventing using other carbon sources. (encyclopedia.pub)
Escherichia coli is capable of utilizing several compounds as carbon sources. (biomedcentral.com)

Bottom): Those animals still testing positive showed a 98 percent reduction in concentrations of E. coli O157. (foodengineeringmag.com)

Carreón-Rodríguez OE, Gosset G, Escalante A, Bolívar F. Dynamics of Glucose Transport in Escherichia coli . (encyclopedia.pub)
Dynamics of Glucose Transport in Escherichia coli " Encyclopedia , https://encyclopedia.pub/entry/46077 (accessed November 28, 2023). (encyclopedia.pub)
Ligand binding and conformational dynamics of the E. coli nicotinamide nucleotide transhydrogenase revealed by hydrogen/deuterium exchange mass spectrometry. (mpg.de)

Experiments to obtain a mutant in the presence of a second copy of impC containing an active-site mutation, in the presence of porin-like gene of M. smegmatis , or in the absence of inositol 1-phosphate synthase activity, were also unsuccessful. (biomedcentral.com)

When asked whether grass/hay feeding prior to harvest can decrease the degree of E. coli shedding as some studies have suggested, Morgan points out that getting definitive information on the efficacy of grass feeding is difficult for two reasons: First, the number of cattle tested in these grain vs. grass studies was limited to a few dozen, which does not yield very conclusive scientific evidence. (foodengineeringmag.com)

SRP, an acronym for siderophore receptors and porins, is a technology that inhibits E. coli's ability to seek and bind iron, which it needs to sustain growth. (foodengineeringmag.com)

However, studies on the carbon stress response of E. coli growing only on an energy-poor carbon source such as glycerol are scarce. (biomedcentral.com)
The current study elucidates and compares the changes in the membrane proteome of Escherichia coli in response to a range of alcohols. (biomedcentral.com)

We have defined a new scheme for annotating sentence-bound gene regulation events, centred on both verbs and nominalised verbs. (biomedcentral.com)
For each event instance, all participants ( arguments ) in the same sentence are identified and assigned a semantic role from a rich set of 13 roles tailored to biomedical research articles, together with a biological concept type linked to the Gene Regulation Ontology. (biomedcentral.com)
Using the scheme, we have created the Gene Regulation Event Corpus (GREC), consisting of 240 MEDLINE abstracts, in which events relating to gene regulation and expression have been annotated by biologists. (biomedcentral.com)
Our work has been concerned with the development of such a corpus for the biomedical field, the Gene Regulation Event Corpus (GREC), consisting of MEDLINE abstracts semantically annotated with event information. (biomedcentral.com)

Waldman SA, O'Hanley P (1989) Influence of a glycine or proline substitution on the functional properties of a 14-amino acid analog of Escherichia coli heat-stable enterotoxin. (springer.com)

One strain had neither porin alteration or increased AmpC production. (cdc.gov)
Glucose is the primary substrate used as the carbon source for laboratory and industrial cultivation of E. coli for production purposes. (encyclopedia.pub)
The production of ethanol by E. coli is preferable as it can metabolize a range of substrates, including the sugars derived from lignocellulosic biomasses readily available from agricultural wastes [ 20 ]. (biomedcentral.com)
When asked whether this seems expensive, he suggested that all the equipment added to production lines to fix the E. coli problem is not exactly cheap, probably hitting close to a million dollars or more per line. (foodengineeringmag.com)
Ikemura H, Takagi H, Inouye M (1987) Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli . (springer.com)

Our approach is based on the fact that many events are focussed on either verbs (e.g., transcribe , regulate ) or nominalised verbs (e.g., transcription , regulation ). (biomedcentral.com)

ETEC_2479 is predicted to function as an outer membrane porin involved in long chain fatty acid transport [ 7 ]. (biomedcentral.com)
Field M, Graf LH Jr, Laird WJ, Smith PL (1978) Heat-stable enterotoxin of Escherichia coli: In vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine. (springer.com)

Of 54 detectable E. coli sRNAs in our microarray analysis, 30 sRNAs showed a differential expression upon HU stress, of which 28 sRNAs displayed a YbeY-dependent change in expression. (biomedcentral.com)

CsgE could establish a paradigm for the regulation of amyloidogenesis because of its unique role in curli formation. (wustl.edu)

While there has been a lot of controversy about grain vs. grass/hay feeding methods to curb E. coli O157:H7 shedding (see below references), a vaccine might be a better preventive measure-at least with more predictable results. (foodengineeringmag.com)

E. coli nonspecifically transports glucose from the extracellular medium into the periplasmic space through the outer membrane porins. (encyclopedia.pub)

Anatomy4

Organisms11

Diseases2

Chemicals and Drugs23

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes24

Information Science3

Health Care1

Strains12

Bacteria9

OmpC3

Klebsiella2

Mutations2

Enterotoxigenic4

Outer membrane proteins2

Proteins4

Bacterial5

Pseudomonas1

Genes3

Lactamase2

Antibiotic1

Mutants1

Isolates2

Responses4

Receptor1

Resistance2

Mycobacterium1

Transporters1

Genome Regulation1

Mechanism3

Clinical1

Carbon sources2

Concentrations1

Dynamics3

Mutation1

Efficacy1

Bind1

Response2

Gene regulation4

Functional1

Production5

Transcription1

Transport2

Expression1

Role1

Methods1

Medium1