Escherichia coli phage. Medical search. Frequent questions

The present study investigated the potential application of the bacteriophage (or phage) phT4A, ECA2 and the phage cocktail phT4A/ECA2 to decrease the concentration of Escherichia coli during the depuration of natural and artificially contaminated cockles. (ua.pt)
Our crystal structures of Phd-Doc from bacteriophage P1, the HigA antitoxin from Escherichia coli CFT073, and YeeU of the YeeUWV systems from E. coli K12 and Shigella flexneri confirm this inference and reveal additional, unanticipated structural relationships. (nih.gov)
Antibody phage display involves the cloning of nucleotide sequences encoding the antibody variable regions and display of the antibody fragments as fusion proteins on the surface of bacteriophage. (usda.gov)
Single chain antibody fragments (scFvs) of desired specificity are selected from a large collection of bacteriophage expressing unique scFvs through the binding of the phage displayed scFv with the target of interest. (usda.gov)
Felix d'Herelle first coined the term "bacteriophage" ( 4 ), literally meaning "bacterium eater," and began using phages to treat bacterial infections in human patients ( Fig. 1 ). (asm.org)
T4Phage is a bacteriophage that infects E. coli bacteria. (cellimagelibrary.org)
Transmission electron microscope image of T4Phage, a bacteriophage that infects Escherichia coli bacteria. (cellimagelibrary.org)
This entry was posted in E. coli and tagged age of aquarius , bacteriophage , e. coli O157 , food safety by Doug Powell . (barfblog.com)
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects susceptible bacterial cells, that is composed of DNA and infects most strains of Escherichia coli. (shapeways.com)
Bacteriophage T7 has a lytic life cycle and several properties that make it an ideal phage for experimentation. (shapeways.com)
The phage standard which we are introducing to the iGEM competition allows basic modification of bacteriophage genomes and must be treated with care. (igem.org)

The E. coli O157 phage typing scheme exhibited a significantly modular network linked to the genetic similarity of each group showing that these groups are specialised to infect a subset of phage types. (biomedcentral.com)
There are a range of phages that infect Escherichia coli that progress either to a lytic or lysogenic phase after infection. (biomedcentral.com)
The results showed that both phages could infect many serotypes of E. coli. (cdc.gov)
Virulent or obligate lytic phages infect and quickly kill their bacterial host cell, whereas temperate or lysogenic phages may either stably integrate into their host's genome or enter into the lytic life cycle. (asm.org)
Filamentous phage use a bacterial structure known as the F pilus to infect E. coli , with the M13 p3 tip contacting the TolA protein on the bacterial pilus. (bionity.com)
Conversely, if the phage mutates to become unable to infect S. aureus , then that phage will fail to infect or replicate. (igem.org)
In conclusion, these experiments showed that the phage was able to infect the E. coli K1 in the system but did not completely eliminate the bacterial load . (bvsalud.org)

A second common laboratory strain is the B strain, whose history is less straightforward and the first naming of the strain as E. coli B was by Delbrück and Luria in 1942 in their study of bacteriophages T1 and T7. (wikipedia.org)
The original E. coli B strain, known then as Bacillus coli, originated from Félix d'Herelle from the Institut Pasteur in Paris around 1918 who studied bacteriophages, who claimed that it originated from Collection of the Institut Pasteur, but no strains of that period exist. (wikipedia.org)
Phage-typing of STEC O157 is a scheme based on the use of 16 bacteriophages that produce a phage infection profile for a strain based on the level of lysis achieved by each phage [ 11 ] and has been used to categorize outbreaks and sporadic cases. (biomedcentral.com)
Bacteriophages (phages) are viruses capable of infecting and replicating within bacterial cells. (asm.org)
We asked the students to come up with a location and methods they wanted to use to check for bacteriophages ("phages" for short), then did some hands-on isolation using non-pathogenic strains of bacteria in our lab. (beacon-center.org)
Although bacteriophages have been studied for over a century, and we've known for awhile that they're abundant, we don't know as much about the diversity or identity of the phages that are out there in the environment. (beacon-center.org)

During the course of a comprehensive study on the phage typing of V. cholerae O1, most strains isolated in Brazil were found to be sensitive with a set of 10 El Tor phages (ATCC 51352-B1-B10) ( 4 ). (cdc.gov)
A total of 107 strains of Vibrio and Enterobacteriaceae were tested against these two phages. (cdc.gov)
All strains were untypeable, except for the O6 and O34 serotypes, which were lysed by the phages specific for O6 (DR1) and O34 (DR2). (cdc.gov)
Escherichia coli is one of the most diverse bacterial species, with several pathogenic strains with different symptoms and with only 20% of the genome common to all strains. (wikipedia.org)
Furthermore, from the evolutionary point of view, the members of genus Shigella (dysenteriae, flexneri, boydii, sonnei) are actually E. coli strains "in disguise" (i.e. (wikipedia.org)
Four of the many E. coli strains (K-12, B, C, and W) are thought of as model organism strains. (wikipedia.org)
Due to the lack of specific record-keeping, the "pedigree" of strains was not available and had to be inferred by consulting lab-book and records in order to set up the E. coli Genetic Stock Centre at Yale by Barbara Bachmann. (wikipedia.org)
The different strains have been derived through treating E. coli K-12 with agents such as nitrogen mustard, ultra-violet radiation, X-ray etc. (wikipedia.org)
While ongoing work is focused on sequencing and analysis of the bacterial strains, we propose that further insight into relevant strain differences can be gained by also understanding the typing phages themselves and the basis of their infection selectivity. (biomedcentral.com)
Little is known about the molecular basis for the interaction between phages and different strains of different phage types, however we can interrogate the phage infection profile of who-infects-whom as a bipartite (two-mode) network. (biomedcentral.com)
Both phages were extensively tested on many strains of Escherichia coli and other enterobacteria. (cdc.gov)
Among the enterobacteria, Proteus mirabilis, Shigella dysenteriae, and two Salmonella strains were lysed by the phages. (cdc.gov)
Yet, most of what we know about E. coli stems from the investigation of laboratory strains, which fail to capture the ecology and evolution of this key organism "in the wild" ( Hobman, Penn & Pallen, 2007 ). (peerj.com)
stx 1, stx 2, eae , and hlyA (or ehx A). Variation in amplification success is likely to increase because more virulence factor variants are certain to emerge as more EHEC and Shiga toxin-producing E. coli (STEC) strains are identified. (cdc.gov)

Pseudomonas phage phi6 infects Gram-negative plant-pathogenic Pseudomonas species [ 7 , 8 ] and is the type member of this family [ 9 ]. (biomedcentral.com)

Escherichia coli O157:H7 is the most prevalent Shiga toxin producing E. coli (STEC) serotype in the UK and has the most severe impact on human health [ 1 ]. (biomedcentral.com)
We used whole-genome sequencing to investigate the evolutionary context of an emerging highly pathogenic strain of Shiga toxin-producing Escherichia coli (STEC) O157:H7 in England and Wales . (barfblog.com)
In many laboratories, sorbitol-MacConkey medium is commonly used to screen for the slow sorbitol fermentation phenotype of the most common Shiga toxin-containing strain: O157:H7 ( 5 ), but this process does not address the pathogenic potential of the remaining sorbitol-positive E. coli . (cdc.gov)

By combining phage therapy and depuration procedures, a reduction in bivalves depuration period can be achieved for, thus decreasing the cost associated with this procedure and even enhance the quality and safety of depurated bivalves destined for human consumption. (ua.pt)

however, bacteria were considered primitive and pre-cellular and received little attention before 1944, when Avery, Macleod and McCarty demonstrated that DNA was the genetic material using Salmonella typhimurium, following which Escherichia coli was used for linkage mapping studies. (wikipedia.org)
In addition, the presence of available nutrients in necrotic tumor tissues attracts facultative anaerobes like Salmonella and Escherichia to the cancerous site via chemotaxis. (nature.com)
RÉSUMÉ L'apparition d'isolats de Salmonella enterica serovar Typhi montrant une résistance à la ciprofloxacine et aux céphalosporines de 3e génération inquiète les médecins des pays en développement. (who.int)

Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. (lu.se)
The aim of this study was to analyse the genome sequences of 16 (fourteen T4 and two T7) E. coli O157 typing phages and to determine the genes responsible for the subtle differences in phage type profiles. (biomedcentral.com)
The typing phages were sequenced using paired-end Illumina sequencing at The Genome Analysis Centre and the Animal Health and Veterinary Laboratories Agency and bioinformatics programs including Velvet, Brig and Easyfig were used to analyse them. (biomedcentral.com)
A lytic phase will cause cell lysis whereas in lysogenic phase the phage becomes integrated into the host genome and becomes a prophage. (biomedcentral.com)
Phage LG1 had a genome size of 49.5 kb compared to 150 kb for AR1. (cdc.gov)
The morphological, genome size, and restriction endonuclease similarities between AR1 and phage T4 were striking. (cdc.gov)
The phage coat is primarily assembled from a 50 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome . (bionity.com)
For example, when the phage genome was mutated to reduce its number of DNA bases (from 6.4 kb to 221 bp) [1] , then the number of p8 copies was decreased to less than 100, causing the p8 coat to shrink in order to fit the reduced genome. (bionity.com)
However, deletion of a phage protein (p3) prevents full escape from the host E. coli , and phage that are 10-20X the normal length with several copies of the phage genome can be seen shedding from the E. coli host. (bionity.com)
Two phage gene products play critical roles in the next stage of the phage life cycle, namely amplification of the genome. (bionity.com)
Without p2, no replication of the phage genome can occur. (bionity.com)
However, since phage genomes generally range from 15 to 150 kilo base pairs so that the genome can fit inside the capsid, the genomes are highly refined and do not contain much redundant DNA available for novel gain-of-function mutations. (igem.org)
STED, stimulated emission depletion IntroductionRibonucleoprotein (RNP) granules are a conserved mechanism to alter granule function is promoted by decapping activators that recruit DCP1 to the WT lambda phage genome mutation. (billfryer.com)
We isolated and whole-genome sequenced up to five colonies of faecal E. coli from 66 asymptomatic children aged three-to-five years in rural Gambia (n = 88 isolates from 21 positive stools). (peerj.com)

In 1885, Theodor Escherich, a German pediatrician, first discovered this species in the feces of healthy individuals and called it Bacterium coli commune because it is found in the colon and early classifications of Prokaryotes placed these in a handful of genera based on their shape and motility (at that time Ernst Haeckel's classification of Bacteria in the kingdom Monera was in place). (wikipedia.org)
Following a revision of Bacteria it was reclassified as Bacillus coli by Migula in 1895 and later reclassified as Escherichia coli. (wikipedia.org)
Specificity of the sensor was demonstrated using common foodborne bacteria and a control phage lacking the scFv. (usda.gov)
Transmission electron microscope image of T4Phage on the surface of Escherichia coli bacteria. (cellimagelibrary.org)
On an agar plate like this, phages kill their host and leave a clear spot where bacteria would have grown ("the circle of death"), known as a plaque. (beacon-center.org)
iPHoP: A Matchmaker for Phages and their Hosts Building on existing virus-host prediction approaches, a new tool combines and evaluates multiple predictions to reliably match viruses with their archaea and bacteria hosts. (doe.gov)
In terms of human or animal safety, this engineered phage should not pose any biohazardous risk since it is specific to bacteria and can already be found in nature albeit without DspB. (igem.org)
if the phage manages to target various bacteria AND DspB also degrades various biofilms AND the quorum-sensing promoter becomes appropriate to various bacteria, resulting in widespread degradation of all types of biofilms without control, THEN this would have some environmental ramifications since biofilms are found on most natural surfaces. (igem.org)
PHAXIAM is developing a portfolio of phages targeting 3 of the most resistant and dangerous bacteria, which together account for more than two-thirds of resistant hospital-acquired infections: Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. (fox4kc.com)

To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. (lu.se)
The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. (lu.se)
The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing, which could be related to phage transcription. (lu.se)
No genes belonging to any known E. coli stress response pathways were scored as upregulated. (lu.se)
The analysis showed that the T7 typing phages (9 and 10) differed by only three genes and that the T4 typing phages formed three distinct groups of similar genomic sequences: Group 1 (1, 8, 11, 12 and 15, 16), Group 2 (3, 6, 7 and 13) and Group 3 (2, 4, 5 and 14). (biomedcentral.com)
Sequencing the typing phage has enabled us to identify the variable genes within each group and to determine how this corresponds to changes in phage type. (biomedcentral.com)
Temperate phages are capable of protecting their host from phage reinfection and may change the bacterial phenotype through the expression of viral genes, a process known as lysogenic conversion ( 1 ). (asm.org)
We demonstrate the utility of our new approach by applying it to identify genes used by Escherichia coli to survive in a wide range of different concentrations of the biocide Triclosan. (biorxiv.org)
This DNA then serves as a template for expression of the phage genes. (bionity.com)
Thus, resident E. coli in these children may constitute reservoirs of virulence- and resistance-associated genes. (peerj.com)

We collected 77 environmental samples from the surroundings of 12 patients with infection/colonizations by Escherichia coli , Staphylococcus aureus or Klebsiella spp. (mdpi.com)
Our project idea is to engineer a Staphylococcus aureus -specific phage that contains DspB, a biofilm matrix-degrading enzyme and is controlled by a S. aureus quorum-sensing system. (igem.org)

Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form. (wikipedia.org)
Phage Targeting Neonatal Meningitis E. coli K1 In Vitro in the Intestinal Microbiota of Pregnant Donors and Impact on Bacterial Populations. (bvsalud.org)
These in vitro cleavage and phage with preexisting mutations may drive further mutation in the target. (billfryer.com)
Escherichia coli is a host bacterium widely used for the production of recombinant proteins, is the cell factory of choice for in vitro studies of phage protein function. (bvsalud.org)

Interestingly there is one additional phage-encoded protein, pX (p10), that is important for regulating the number of double stranded genomes in the bacterial host. (bionity.com)
This procedure allows cell-free cloning of individual synthetic DNA molecules that cannot be cloned in Escherichia coli, for example synthetic phage genomes carrying lethal mutations. (jcvi.org)
Also, this study adds significantly to the number of commensal E. coli genomes, a group that has been traditionally underrepresented in the sequencing of this species. (peerj.com)

If the quorum-sensing promoters that control the expression of the phage and DspB mutate to become constitutive or incorrectly activated without the presence of a biofilm, then the phage will simply lyse its host prematurely. (igem.org)

Shiga toxin producing Escherichia coli O157 can cause severe bloody diarrhea and haemolytic uraemic syndrome. (biomedcentral.com)
Enterohemorrhagic Escherichia coli (EHEC) pathogenicity is usually linked to a Shiga toxin ( 1 , 2 ) and virulence factors, including adhesins, toxins, invasins, protein secretion systems, iron uptake systems, and several unidentified functions ( 3 , 4 ), which are unrelated to strain phylogeny. (cdc.gov)

A longer term aim of the work is to understand the factors that mediate resistance and susceptibility in the phage-bacterium relationship. (biomedcentral.com)
The head, sheath, and long leg fibers will assemble into progeny phages within the infected E. coli bacterium. (biolution.net)

The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. (biomedcentral.com)
This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
Early studies on nonsense mutants of phage phi6 suggested that the major envelope protein P9 and the non-structural protein P12 are the only proteins needed for phi6 virion envelopment [ 23 ]. (biomedcentral.com)
There are four other proteins on the phage surface, two of which have been extensively studied. (bionity.com)
These form the rounded tip of the phage and are the first proteins to interact with the E. coli host during infection. (bionity.com)
Purification of proteins from natural phages is often time-consuming, cumbersome and low yielding. (bvsalud.org)

phage particles of LG1 were smaller than those of AR1 and had an isometric head 68 nm in diameter and a complex contractile tail 111 nm in length. (cdc.gov)
Transmission electron micrographs of AR1 showed phage particles consisting of an elongated isometric head of 103 by 74 nm and a complex contractile tail 116 nm in length. (cdc.gov)
pV (aka p5) competes with double stranded DNA formation by sequestering copies of the + stranded DNA into a protein/DNA complex destined for packaging into new phage particles. (bionity.com)
Single-mutant phages were used for the analysis of DCP1 overexpression in the Supporting information figures contain replicates that were exposed to enough phage particles (Fig 5B). (billfryer.com)

Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. (yale.edu)

Morphological, host range, and genetic characterization of two coliphages. (cdc.gov)

Antibody phage display is a molecular method for the selection of antibody fragments of desired specificity and is an alternative to traditional means for the generation of polyclonal or monoclonal antibodies. (usda.gov)

one section was used to isolate O1 phages using MAK 757 (ATCC 51352) as a propagating strain. (cdc.gov)
The phages were purified from a single discrete plaque by the soft agar (0.8%) overlay method with the propagating strain of V. cholerae non-O1 (O2 to O139) until homogenous plaques were obtained. (cdc.gov)
Phage lysates were prepared in nutrient broth (Difco Laboratories, Detroit, MI) with the propagating strain V. cholerae (both O1 and non-O1). (cdc.gov)
In the course of the passages it lost its O antigen and in 1953 was cured first of its lambda phage (strain W1485 Archived 2011-07-25 at the Wayback Machine by UV by Joshua Lederberg and colleagues) and then in 1985 of the F plasmid by acridine orange curing. (wikipedia.org)
An extensive list of Escherichia coli K-12 strain derivatives and their individual construction, genotypes, phenotypes, plasmids and phage information can be viewed at Ecoliwiki. (wikipedia.org)
Although clinical microbiology typically relies on single-colony picks (which has the potential to underestimate species diversity and transmission events), within-host diversity of E. coli in the gut is crucial to our understanding of inter-strain competition and co-operation and also for accurate diagnosis and epidemiological analyses. (peerj.com)
1989 were 100% sensitive to gentamicin, Escherichia coli ATCC 25922 strain was and there was clinical response for 60% used as the control. (who.int)

Hence, we conclude that CQD-CRISPR-Cas9-gRNA could be a novel therapeutic strategy for the eradication or treatment of antibiotic resistant pathogenic E. coli -based pathologies. (microbiologyresearch.org)

High-titer phages were obtained by the plate lysis procedure by using the agar overlay method ( 5 ) with multiplicity of infection 0.01 at an incubation temperature of 37°C. Concentration, purification, and electron microscopy study of these phages were performed as described by Ghosh et al. (cdc.gov)
T7 has a short life cycle of 17 min at 37˚C, i.e. the time from infection to the lysis of the host cell when new phage are released. (shapeways.com)
We observed a strong promoter, causing eventual lysis of cultures infected with phage diluted 1:8. (billfryer.com)

If DspB mutates and gains the ability to degrade a greater variety of biofilms, the mutation may not be uncommon in nature although the fact that it is propagated by phage may increase the mutation frequency. (igem.org)
The probability of this happening is moderate based on the rate of mutation in phages and the numbers of phages produced per infection. (igem.org)

Several isolates encode putative virulence factors commonly found in Enteropathogenic and Enteroaggregative E. coli, and 53% of the isolates encode resistance to three or more classes of antimicrobials. (peerj.com)
We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. (cdc.gov)

Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli . (bionity.com)

Surface swabs were tested for lytic phages and bacterial isolates for mitomycin C-inducible prophages. (mdpi.com)

It should also be considered that if phage DNA mutates to be harmful in some way, the potential spread is greater because every phage is capable of up to 200 progeny. (igem.org)

A plasmid vector was constructed that allowed the expression of biotinylated scFvs in Escherichia coli. (usda.gov)

Here, we report an efficient gene editing strategy based on direct delivery of Cas9 and gRNA into Uropathogenic E. coli (UPEC) isolate. (microbiologyresearch.org)

RESULTS: We expressed the gene encoding Enterococcus faecium phage IME199 DNA polymerase (IME199 DNAP) in Escherichia coli BL21(DE3), and characterized protein function. (bvsalud.org)
CONCLUSIONS: A novel family B DNA polymerase was successfully overproduced in Escherichia coli BL21(DE3). (bvsalud.org)

The experimental part focus on the model organisms, Bacillus subtilis , Escherichia coli and Streptomyces coelicolor . (lu.se)

Prophages are important as they often encode additional factors not directly linked to phage production that may provide an evolutionary advantage to the bacterial host enabling survival of the embedded prophage. (biomedcentral.com)

Now, with the rise of antibiotic resistance, phage therapy is experiencing a well-deserved rebirth. (asm.org)
We cover the key points of the antibiotic resistance crisis and then explain the biological and evolutionary principles that support the use of phages, their interaction with the immune system, and a comparison with antibiotic therapy. (asm.org)

By spring, we'd already planned to repeat the activity in the same grad course and were trying to bring phage isolation to undergraduate biology labs at MSU. (beacon-center.org)

A variety of enteropathogens were included for susceptibility against these phages. (cdc.gov)

Restriction endonuclease analysis showed that several restriction enzymes could degrade DNA from both phages. (cdc.gov)
We discuss conventional approaches as well as novel strategies, including the use of phage-antibiotic combinations, phage-derived enzymes, exploitation of phage resistance mechanisms, and phage bioengineering. (asm.org)
Host enzymes copy the replicated + strand, resulting in more copies of double stranded phage DNA. (bionity.com)
As such, it represents a new fold for Hj-resolving enzymes from phages. (iucr.org)

An enterohaemorrhagic Escherichia coli (EHEC) outbreak at an institute with multiple facilities for children and adults with intellectual disabilities was investigated to characterize the cases and identify risk factors for infection. (who.int)
Enterohaemorrhagic Escherichia coli (EHEC) was first The objectives of the study were to characterize the reported in 1983 in the United States of America. (who.int)

The homogeneity of each phage was studied by plaque morphology on nutrient agar following 10-fold serial dilution. (cdc.gov)

Since 2007, phages have been used sporadically to treat bacterial infections in well-defined cases in the Queen Astrid military hospital (QAMH) in Brussels, Belgium. (mdpi.com)

If we had to break through the bounds of imagination to imagine the absolute worst case apocalyptic scenario ever, maybe our phage will mutate into a human-specific virus AND DspB will become able to degrade various polymers in humans AND the quorum-sensing promoter will become a constitutive promoter so that our phage will wipe out the human race. (igem.org)
Next, we harvested phage from the stronger promoter. (billfryer.com)

If our phage does mutate to become more promiscuous, there are still many barriers in place to prevent it from effectively eliminating other species' biofilms. (igem.org)

Little is known about the genomic diversity of Escherichia coli in healthy children from sub-Saharan Africa, even though this is pertinent to understanding bacterial evolution and ecology and their role in infection. (peerj.com)

Thus, global transcriptional analysis and functional analysis revealed previously unknown host responses to filamentous phage infection. (lu.se)
The wide host ranges of phages AR1 and LG1 suggest that they may be useful as biocontrol, therapeutic, or diagnostic agents to control and detect the prevalence of E. coli in animals and food. (cdc.gov)
Although we've only been doing this kind of isolation for about a year, we've already discovered a total of 36 new phages, some of which are pretty rare and/or have really unique qualities (like the broad host range mentioned above). (beacon-center.org)
The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli . (bionity.com)
p3 is also the last point of contact with the host as new phage bud from the bacterial surface. (bionity.com)
Despite the importance of virus-host interactions and likely variable infection efficiencies of individual phages across hosts, such variability is seldom quantified. (doe.gov)
The phage will still be under the control of the S. aureus quorum-sensing system, and it will unlikely be expressed/triggered in its new infected host. (igem.org)
The results showed that phage vB_EcoP_K1_ULINTec4, inoculated alone, was progressively cleared by the system and replicates in the presence of its host. (bvsalud.org)
These were spread over 37 seven-allele sequence types and the E. coli phylogroups A, B1, B2, C, D, E, F and Escherichia cryptic clade I. Immigration events accounted for three-quarters of the diversity within our study population, while one-quarter of variants appeared to have arisen from within-host evolution. (peerj.com)

This study demonstrates the applicability of antibody fragments selected via phage display for the development of immunoreagents and the detection of foodborne pathogens. (usda.gov)

From April 2013 to April 2018, 260 phage therapy requests were addressed to the QAMH. (mdpi.com)

A Sensitive Voltammetric Biosensor for Escherichia Coli Detection Using an Electroactive Substrate for ß -D-Glucuronidase. (donau-uni.ac.at)
8-hydroxyquinoline-glucuronide sodium salt used as electroactive substrate for a sensitive voltammetric detection of Escherichia coli in water samples. (donau-uni.ac.at)
An automated, Robotic Biosensor for the Electrochemical Detection of E. Coli in Water. (donau-uni.ac.at)
A fluorescence based sensor system for automated detection of E. coli in water. (donau-uni.ac.at)
Electrochemical detection of Escherichia coli based on beta-D-glucuronidase enzyme activity. (donau-uni.ac.at)
Development of an Automated Biosensor for Rapid Detection and Quantification of E. coli in Water. (donau-uni.ac.at)
Development of a biosensor for the amperometric phage-mediated detection of Escherichia coli in aqueous samples. (donau-uni.ac.at)

Using transmission electron microscopy, we detected membranous structures in the cytoplasm of E. coli cells expressing P9. (biomedcentral.com)

DspB is also only known to degrade S. aureus and Escherichia coli biofilms. (igem.org)

The phage-infected cells demonstrated impaired function of the oxidative and the glutamate-dependent acid resistance systems. (lu.se)
UTI, although treatable, is now becoming increasingly tough to control because of rampant antimicrobial resistance in the Enterobacteriaceae family, particularly inEscherichia coli. (microbiologyresearch.org)
We'll be discussing trends of antimicrobial resistance in E. coli . (cdc.gov)
Sarah Gregory] Your study is about antimicrobial resistance (or AMR) among E. coli . (cdc.gov)

Phages replicate through two primary life cycles, the dynamics of which have important implications for their therapeutic application. (asm.org)

In the last two years, external requests for phage therapy have increased significantly. (mdpi.com)

Immunization of mice with purified f88-M2e2-16 phages in the presence of incomplete Freund's adjuvant, induced robust M2e-specific serum IgG and protected BALB/c mice against challenge with human and avian influenza A viruses. (uu.nl)
The company is building on an innovative approach based on the use of phages, natural bacterial-killing viruses. (krqe.com)

We show that the resulting recombinant f88-M2e2-16 phages are replication competent and display the introduced part of M2e on the phage surface. (uu.nl)

Phage typing of E. coli O157 facilitates public health surveillance and outbreak investigations, certain phage types are more likely to occupy specific niches and are associated with specific age groups and disease severity. (biomedcentral.com)

Samples were processed for phage isolation according to the procedure adopted for V. cholerae phages ( 5 , 6 ). (cdc.gov)
After taking him through the isolation procedure, we made a plan for how he could have his students isolate their own phages back in his classroom. (beacon-center.org)

However, when employing the phage cocktail, a longer treatment time was required to obtain comparable results to those achieved when using single phage suspensions. (ua.pt)
On collection day, each of the 50 students got their own sterile tube and went to a place they thought would most likely have phage, or at least somewhere they thought might have interesting results. (beacon-center.org)
Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. (biomedcentral.com)
The probability of this happening is moderate considering the great number of phages produced during each infection, but the results are not hazardous as explained. (igem.org)

Anatomy8

Organisms36

Diseases7

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes79

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care5

Bacteriophage11

Infect7

Bacteriophages6

Strains14

Infects1

O157:H73

Combining phage therapy1

Salmonella3

Genome14

Bacteria9

Genes10

Staphylococcus2

Vitro4

Genomes3

Expression of the phage1

Shiga2

Bacterium2

Proteins6

Particles4

Mutations1

Characterization1

Molecular1

Strain7

Treatment of Antibiotic-Resistant1

Lysis3

Mutation2

Virulence factors2

Lethal1

Isolates1

Progeny1

Plasmid1

Uropathogenic1

BL212

Bacillus1

Bacterial host1

Antibiotic resistance2

Biology1

Susceptibility1

Enzymes4

EHEC2

Plaque morphology1

Treat bacterial infections1

Promoter2

Species1

Genomic1

Host9

Antibody1

20181

Detection7

Electron1

Degrade1

Resistance4

Replicate1

Significantly1

Viruses2

Replication1

Outbreak1

Isolation2

Results4