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Malate SynthaseMalate DehydrogenaseMalatesEscherichia coli ProteinsEscherichia coliEscherichia coli InfectionsMalate Dehydrogenase (NADP+)Molecular Sequence DataEscherichia coli O157Bacterial ProteinsGenes, BacterialL-Lactate DehydrogenaseAmino Acid SequenceBase SequenceKineticsCloning, MolecularOxaloacetatesPlasmidsIsocitrate DehydrogenaseMutationDNA, BacterialNADEscherichia coli K12Oxaloacetic AcidAlcohol DehydrogenaseGlutamate DehydrogenaseSuccinate DehydrogenaseGene Expression Regulation, BacterialAlcohol OxidoreductasesGlucosephosphate DehydrogenaseGlyceraldehyde-3-Phosphate DehydrogenasesAdhesins, Escherichia coliOperonRecombinant ProteinsCitrate (si)-SynthaseKetoglutarate Dehydrogenase ComplexGlycerolphosphate DehydrogenaseSubstrate SpecificityCitric Acid CycleSequence Homology, Amino AcidOxidoreductasesBinding SitesAldehyde DehydrogenaseHydrogen-Ion ConcentrationRNA, BacterialChromosomes, BacterialBotanyGlutamate SynthaseMedicago sativaTransaminasesGlutamate-Ammonia LigaseEntomophthora
Lactate dehydrogenaseO157:H7MitochondrialPyruvateCitric acidSubunitAlcohol dehydrogenaseMitochondriaGlyceraldehydeSuccinateEnzymeEnzymesProteinsSubstrateSynthesisGenesPathwayTransgenicOxaloacetateGeneKlebsiellaCytoplasmicDnaKOxidationSolanumListeriaArchaeonComplexesPopulationsGlycerolIsozymesCorynebacteriumStrainCatalyzesBacteriaReceptorIsolatesRegulatoryCarbonMechanisms
Lactate dehydrogenase7
  • Humans and most other mammals express the following two malate dehydrogenases: The malate dehydrogenase family contains L-lactate dehydrogenase and L-2-hydroxyisocaproate dehydrogenases. (wikipedia.org)
  • In most organisms, malate dehydrogenase (MDH) exists as a homodimeric molecule and is closely related to lactate dehydrogenase (LDH) in structure. (wikipedia.org)
  • This indicates that there is a possible evolutionary linkage between lactate dehydrogenase and malate dehydrogenase. (wikipedia.org)
  • deletion of the hypoxanthine phosphoribosyltransferase gene in ethanol tolerant strain adhE*(EA), carrrying mutation P704L/H734R in the alcohol dehydrogenase gene, and deletion of lactate dehydrogenase (ldh) to redirect carbon flux towards ethanol reults in a strain producing 30% more ethanol than wild type on minimal medium. (brenda-enzymes.info)
  • First, molecular dynamics were analyzed for the hyperthermophile malate dehydrogenase from Methanococcus jannaschii and a mesophilic homologue, the lactate dehydrogenase from Oryctolagus cunniculus muscle. (waikato.ac.nz)
  • Assessing the impact of 4-oxo-2-nonenal on lactate dehydrogenase activity and myoglobin redox stability. (uga.edu)
  • Recent studies with the isozymes of lactate dehydrogenase have demonstrated that LDH-1, the LDH isozyme which moves rapidly in an electrophoretic field, is particularly helpful in confirming the diagnosis of myocardial infarction. (eurekamag.com)
O157:H74
  • Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. (cdc.gov)
  • An evolutionary model postulates that Escherichia coli O157:H7 evolved from ancestral E. coli by stepwise acquisition or loss of virulence and phenotypic traits ( 1 ). (cdc.gov)
  • Survival of Escherichia coli O157:H7 and Listeria monocytogenes in an acidified low-fat mayonnaise-based hibachi sauce. (uga.edu)
  • Role of organic acids and peroxyacetic acid as antimicrobial intervention for controlling Escherichia coli O157:H7 on beef trimmings. (uga.edu)
Mitochondrial3
  • One is found in the mitochondrial matrix, participating as a key enzyme in the citric acid cycle that catalyzes the oxidation of malate. (wikipedia.org)
  • The other is found in the cytoplasm, assisting the malate-aspartate shuttle with exchanging reducing equivalents so that malate can pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. (wikipedia.org)
  • Enhanced photosynthetic performance and growth as a consequence of decreasing mitochondrial malate dehydrogenase activity in transgenic tomato plants. (mpg.de)
Pyruvate3
  • L-lactate dehydrogenases catalyzes the conversion of L-lactate to pyruvate, the last step in anaerobic glycolysis. (wikipedia.org)
  • To augment ethanol production by the ET strain, an alternative ethanol production pathway comprised of pyruvate decarboxylase (Pdc) and alcohol dehydrogenase (AdhB) from Zymomonas mobilis (Fig. 4A) was transferred into C. phytofermentans on the replicating pQexpE plasmid (Fig. 4B). (researchgate.net)
  • chaperone protein DNak and ftsz in S. aureus and pyruvate kinase, 6-phosphogluconate dehydrogenase, fructose bisphosphate were among the enzymes absent in C.albican cultures. (allaboutheaven.org)
Citric acid1
Subunit1
Alcohol dehydrogenase3
Mitochondria1
  • Because the sequence identity of malate dehydrogenase in the mitochondria is more closely related to its prokaryotic ancestors in comparison to the cytoplasmic isozyme, the theory that mitochondria and chloroplasts were developed through endosymbiosis is plausible. (wikipedia.org)
Glyceraldehyde2
  • The influence of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) on potato tuber metabolism. (mpg.de)
  • Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. (yale.edu)
Succinate2
  • 3 2 Under physiological hypoxia, low oxygen levels lead to reduced activity of succinate dehydrogenase (SDH), which metabolizes succinate, and other oxygen-dependent enzymes in the electron transport chain, causing succinate accumulation. (haematologica.org)
  • Diarrhea-predominant IBS (IBS-D) demonstrated shifts in the metatranscriptome and metabolome including increased bile acids, polyamines, succinate pathway intermediates (malate, fumarate), and transcripts involved in fructose, mannose, and polyol metabolism compared to constipation-predominant IBS (IBS-C). A classifier incorporating metabolites and gene-normalized transcripts differentiated IBS-D from IBS-C with high accuracy (AUC 0.86). (biomedcentral.com)
Enzyme3
  • Malate dehydrogenase (EC 1.1.1.37) (MDH) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. (wikipedia.org)
  • Studies have also identified a mobile loop in malate dehydrogenase that participates in the catalytic activity of the enzyme. (wikipedia.org)
  • The enzyme is therefore called malate:quinone oxidoreductase, abbreviated to Mqo. (rhea-db.org)
Enzymes1
  • However, these results have been underestimated because genes for the two key enzymes of the cycle, isocitrate lyase (ICL) and malate synthase (MS), are not annotated in Leishmania genomes. (degruyter.com)
Proteins1
  • The derived protein sequence is similar to hypothetical proteins from Escherichia coli, Klebsiella pneumoniae, and Mycobacterium tuberculosis. (rhea-db.org)
Substrate2
  • The active site of malate dehydrogenase is a hydrophobic cavity within the protein complex that has specific binding sites for the substrate and its coenzyme, NAD+. (wikipedia.org)
  • The loop undergoes a conformational change to shield the substrate and catalytic amino acids from the solvent in response to the binding of the malate dehydrogenase:coenzyme complex to substrate. (wikipedia.org)
Synthesis2
  • Overexpression of malate dehydrogenase in transgenic alfalfa enhanced organic acid synthesis and confers tolerance to aluminum. (usda.gov)
  • We co-opted Escherichia coli for biomolecular synthesis and assembly of this nanomaterial by expressing and maturing the EcHyd-1 hydrogenase prior to expression of the P22 coat protein, which subsequently self assembles. (nature.com)
Genes3
  • A study of Escherichia coli physiology during growth on glycerol was performed combining transcriptional-proteomic analysis as well as kinetic and stoichiometric evaluations in the strain JM101 and certain derivatives with important inactivated genes. (biomedcentral.com)
  • The expression of metabolic genes, in particular the overexpression of pykA pckA, gltA fumABC sdh mdh and acnA genes and the downregulation of the ackA gene, has been reported for E. coli growing on glycerol [ 7 ]. (biomedcentral.com)
  • 2005. Characterization of TetD as a transcriptional activator of a subset of genes of the Escherichia coli SoxS/MarA/Rob regulon. . (umass.edu)
Pathway2
  • We first functionally demonstrate the design of the MCG pathway in vitro and in Escherichia coli. (osti.gov)
  • 3-phospho-ᴅ-glycerate dehydrogenases (PGDH, or SerA) are involved in the first step of the phosphorylated pathway of L-serine biosynthesis from 3-phosphoglycerate, an intermediate of glycolysis. (bioreproducibility.org)
Transgenic1
  • Cloning and characterization of red clover polyphenol oxidase cDNAs and expression of active protein in Escherichia coli and transgenic alfalfa. (usda.gov)
Oxaloacetate1
  • It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. (rhea-db.org)
Gene2
Klebsiella4
  • In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae ( Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae ) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. (who.int)
  • The isolates appear clonally unrelated with MLST, revealing a diversity of clonal types among the Klebsiella pneumoniae and Escherichia coli isolates. (who.int)
  • The first carbapenem-resistant NDM-1 isolates characterized in 2009 were Klebsiella pneumoniae and Escherichia coli isolated from a Swedish patient who had sought medical care in New Delhi, India. (who.int)
  • The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)
Cytoplasmic2
DnaK1
  • Using DnaK from Escherichia coli and heat-induced aggregation of malate dehydrogenase, we describe a protocol for absorbance and fluorescence-based miniaturized versions of the standard aggregation suppression assay that are affordable and have wide application for low abundance holdases. (bvsalud.org)
Oxidation1
Solanum1
  • Expression of an Escherichia coli phosphoglucomutase in potato (Solanum tuberosum L.) results in minor changes in tuber metabolism and a considerable delay in tuber sprouting. (mpg.de)
Listeria1
  • Using antibodies, we demonstrated that the regulation of both Listeria monocytogenes PPDK and Escherichia coli PEP synthetase involves the phosphorylation of a threonine residue located close to the catalytic histidine residue. (scirp.org)
Archaeon1
Complexes1
  • This work reports the crystal structures of human D-2-HGDH in apo form and in complexes with D-2-HG, D-malate, D-lactate, L-2-HG, and 2-oxoglutarate, respectively. (rcsb.org)
Populations1
  • The genetic code is universal i.e. [39] In large populations of asexually reproducing organisms, for example, E. coli, multiple beneficial mutations may co-occur. (mercinewyork.com)
Glycerol3
  • E. coli growing aerobically on glycerol incorporates this molecule into central metabolism as dihydroxyacetone phosphate (DHAP), a metabolite which can participate in both gluconeogenic and glycolytic processes (Figure 1 ) [ 6 ]. (biomedcentral.com)
  • However, studies on the carbon stress response of E. coli growing only on an energy-poor carbon source such as glycerol are scarce. (biomedcentral.com)
  • This strain grows very poorly on glycerol plus malate unless the medium is supplemented with casamino acids. (yale.edu)
Isozymes1
Corynebacterium1
Strain1
  • For example, the in vivo functionality of prokaryotic JDPs can be tested on the basis of their ability to reverse the thermosensitivity of a dnaJ cbpA mutant strain of the bacterium Escherichia coli (OD259). (bvsalud.org)
Catalyzes1
  • that is, maize leaf PDRP only catalyzes the regulation of PPDK and E. coli PSRP only catalyzes the regulation of PEPS. (scirp.org)
Bacteria1
  • In this study, Cell Counting Kit-8 (CCK-8) was introduced to detect the concentration of live bacteria for the first time depending on the redox reaction between CCK-8 solution and dehydrogenase. (isogem.org)
Receptor1
  • Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA. (ibs.fr)
Isolates1
  • Fourteen E. coli isolates were taken from cattle and pigeon fecal samples and sixteen E. coli isolates were taken from wetland water samples. (uconn.edu)
Regulatory1
  • The PEP synthetase regulatory protein (PSRP) from E. coli has been cloned and expressed [11]. (scirp.org)
Carbon1
  • Escherichia coli is capable of utilizing several compounds as carbon sources. (biomedcentral.com)
Mechanisms1
  • Tanaka, S., Sawaya, M. R. & Yeates, T. O. Structure and mechanisms of a protein-based organelle in Escherichia coli . (nature.com)