Escherichia coli chromosomal gene. Medical search. Frequent questions

In Salmonella , five T6SS gene clusters have been identified within pathogenicity islands SPI-6, SPI-19, SPI-20, SPI-21, and SPI-22, which are differentially distributed among serotypes. (frontiersin.org)
Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. (frontiersin.org)
No mutagenic effect was reported in other investigations using Salmonella typhimurium or Escherichia coli strains and concentrations from 125 - 4000 µg/plate (Dean et al. (europa.eu)
DEA did not induce reverse mutations in Salmonella typhimurium or Escherichia coli. (europa.eu)
It is not mutagenic to Salmonella typhimurium or Escherichia coli . (inchem.org)
Deiwick J, Nikolaus T, Erdogan S, Hensel M: Environmental regulation of Salmonella pathogenicity island 2 gene expression. (pcna-signal.com)
Virulence plasmids borne by serovars of Salmonella enterica carry genes involved in its pathogenicity, as well as other functions. (springeropen.com)
Oligonol did not show any potential to induce gene mutations in reverse mutation tests using Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2 uvr A strains. (breakawaymatcha.com)

B) Phylogenetic tree (unrooted) of the Escherichia coli sequence type 410 isolates harboring the bla CTX-M-15 transposition unit in the chromosome and a schematic depiction of the location of the mcr-1 and bla CTX-M-15 transposition unit. (cdc.gov)
We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. (cdc.gov)
To analyze the genetic diversity of pathogenic and commensal E. coli isolates, a whole-genome approach was applied. (pasteur.fr)
Using DNA arrays, the presence of all translatable open reading frames (ORFs) of nonpathogenic E. coli K-12 strain MG1655 was investigated in 26 E. coli isolates, including various extraintestinal and intestinal pathogenic E. coli isolates, 3 pathogenicity island deletion mutants, and commensal and laboratory strains. (pasteur.fr)
DNA sequences described for extraintestinal or intestinal pathogenic E. coli are more frequently detectable in isolates of the same origin than in other pathotypes. (pasteur.fr)
Several genes coding for virulence or fitness factors are also present in commensal E. coli isolates. (pasteur.fr)
In China, the presence of the qnr gene in the clinical isolates of Enterobacteriaceae has been reported, but this transmissible quinolone resistance gene has not been detected in strains isolated singly from pediatric patients. (biomedcentral.com)
This study therefore aimed to investigate the presence of the qnr gene in clinical isolates of E. coli and K. pneumoniae from pediatric patients in China. (biomedcentral.com)
A total 213 of non-repetitive clinical isolates resistant to ciprofloxacin from E. coli and K. pneumoniae were collected from hospitalized patients at five children's hospital in Beijing, Shanghai, Guangzhou, and Chongqing. (biomedcentral.com)
The isolates were screened for the plasmid-mediated quinolone resistance genes of qnrA , qnrB , and qnrS by PCR. (biomedcentral.com)
The study found that 19 ciprofloxacin-resistant clinical isolates of E. coli and K. pneumoniae were positive for the qnr gene, and most of the qnr positive strains were ESBL producers. (biomedcentral.com)
Therefore, the objective of this study was to screen for the presence of the qnr gene in clinical ciprofloxacin-resistant isolates of Escherichia coli and Klebsiella pneumoniae from pediatric patients in China. (biomedcentral.com)
Three hundred thirty-five Escherichia coli and 392 Klebsiella pneumoniae non-replicate clinical isolates were collected from five children's hospitals located in Beijing, Shanghai, Guangzhou, and Chongqing from January 2005 to December 2006. (biomedcentral.com)
The screening showed 146 Escherichia coli and 67 Klebsiella pneumoniae isolates were ciprofloxacin-resistant. (biomedcentral.com)
In this study, we investigated the presence of plasmid-mediated quinolone resistance (PMQR) genes among 101 ciprofloxacin-resistant urinary Escherichia coli isolates and searched for mutations in the quinolone-resistance-determining regions (QRDRs) of the DNA gyrase and topoisomerase IV genes in PMQR-carrying isolates. (scielo.br)
Eight isolates harboured the qnr and aac(6')-Ib-cr genes (3 qnrS1, 1 qnrB19 and 4 aac(6')-Ib-cr). (scielo.br)
This is the first report of qnrB19, qnrS1 and aac(6')-Ib-cr -carrying E. coli isolates in Brazil. (scielo.br)
2010). However, in the last few decades, an increase in quinolone resistance has been documented among human and veterinary isolates of E. coli . (scielo.br)
Therefore, in this study, we investigated the presence of the qnrA , qnrB , qnrS and aac(6-)-Ib-cr genes in a sample of ciprofloxacin-resistant E. coli isolates from women with CA-UTIs. (scielo.br)
In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae ( Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae ) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. (who.int)
The isolates appear clonally unrelated with MLST, revealing a diversity of clonal types among the Klebsiella pneumoniae and Escherichia coli isolates. (who.int)
The first carbapenem-resistant NDM-1 isolates characterized in 2009 were Klebsiella pneumoniae and Escherichia coli isolated from a Swedish patient who had sought medical care in New Delhi, India. (who.int)
The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)

Enterohemorrhagic Escherichia coli (EHEC) pathogenicity is usually linked to a Shiga toxin ( 1 , 2 ) and virulence factors, including adhesins, toxins, invasins, protein secretion systems, iron uptake systems, and several unidentified functions ( 3 , 4 ), which are unrelated to strain phylogeny. (cdc.gov)

In mammalian in vitro systems, DEA did not induce chromosomal aberrations in rat hepatocytes, sister chromatid exchange or chromosomal aberrations in Chinese hamster ovary cells and gene mutation in mouse lymphoma cells. (europa.eu)
A possible increase in the frequency of chromosomal aberrations was seen in one study, and an increased frequency of sister chromatid exchanges occurred in one group of workers in the other. (inchem.org)
Sequence analysis of Wnt-independent organoids reveals an enhanced mutational burden, including chromosomal aberrations typical of genomic instability. (uni-muenchen.de)
Oligonol did not induce chromosomal aberrations in cultured Chinese hamster lung cells, but it showed increased polyploidy. (breakawaymatcha.com)

We analyzed the proteins induced in Escherichia coli by exposure to FQs, then examined the susceptibilities to these agents of E. coli strains with mutations in the genes encoding these proteins. (biomedcentral.com)
Resistance to quinolones occurs through chromosomal mutations in the genes encoding these enzymes and by porin and efflux mutations. (cdc.gov)

We demonstrated that Escherichia coli cells exposed to levofloxacin (LVFX), a fluoroquinolone (FQ), induce the syntheses of heat shock proteins and RecA. (biomedcentral.com)
Genotoxic colibactin-producing pks+ Escherichia coli induce DNA double-strand breaks, mutations, and promote tumor development in mouse models of colorectal cancer (CRC). (uni-muenchen.de)

Expression was not reported, and the purpose of the study was to show that the technique could be used to study gene action during mammalian development. (justia.com)
The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)

We have previously established that the spontaneous, genome-wide rate of gene duplication in C. elegans is two orders of magnitude higher than the point mutation rate [ 8 ]. (biomedcentral.com)
Each mutation reproduced the mutualistic phenotypes when introduced into wild-type E. coli , confirming that single carbon catabolite repression mutations can make E. coli an insect mutualist. (nature.com)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

This report on transferable fluoroquinolone resistance due to the qnr gene among E. coli and K. pneumoniae strains indicated that plasmid-mediated quinolone resistance has emerged in pediatric patients in China. (biomedcentral.com)
qnrA is the plasmid-mediated quinolone resistance gene encoding a 218 amino acid protein of the pentapeptide family that protects DNA gyrase from quinolone inhibition[ 4 ]. (biomedcentral.com)

This includes an altered microbiome dominated by Enterobacteriaceae and in particular adherent-invasive E. coli (AIEC). (nature.com)

AMR, through acquired genes located on transposons or conjugative plasmids, is the horizontal transmission of genes required for a given bacteria to withstand antibiotics. (mdpi.com)
Isolation of chromosomal mutations that affect carotenoid production in Escherichia coli: mutations alter copy number of ColE1-type plasmids. (igem.org)
The bla NDM-1 gene was encoded on plasmids that were easily transferable. (who.int)

Up to 10% of the E. coli K-12-specific ORFs were not detectable in the genomes of the different strains. (pasteur.fr)

The rates of spontaneous gene duplication and deletion are extraordinarily high and speak to the enormous potential of these structural variants for generating new adaptive variability [ 5 - 10 ]. (biomedcentral.com)
The resultant pseudo F plasmid is transferred by conjugation to the newly established single gene deletion mutant and selected Hfr strain integrated F plasmid by antibiotic resistance selection. (cam.ac.uk)
Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV. (biomedcentral.com)

Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. (frontiersin.org)
Genetic and metabolic analyses of Escherichia coli strains showed that overexpression of PanB leads to accumulation of the likely folate cleavage product 6-hydroxymethylpterin and other pterins in cells and medium, and-unexpectedly-to a 46% increase in total folate content. (ebi.ac.uk)
Transcriptomic and genomic analyses of 'mutualistic' E. coli lines revealed independent mutations that disrupted the carbon catabolite repression global transcriptional regulator system. (nature.com)
I would like to summarize our analyses after genome project of E. coli and will report the present situation in Systems approaches, focusing on genetic interaction. (cam.ac.uk)

大腸菌 / DNA複製 / DNA replication / DNAポリメラーゼ / ATP / DnaA / Escherichia coli / DnaA protein / 細胞分裂 / Fプラスミド / 細胞周期 / ゲノム自己増殖の終結 / ゲノム自己増殖の開 (nii.ac.jp)
Recent progresses in BAC cloning and mutagenesis technology make it possible to identify specific genes important for MDV replication and oncogenesis. (biomedcentral.com)

stx 1, stx 2, eae , and hlyA (or ehx A). Variation in amplification success is likely to increase because more virulence factor variants are certain to emerge as more EHEC and Shiga toxin-producing E. coli (STEC) strains are identified. (cdc.gov)
Pathogenic and nonpathogenic E. coli strains differ in the presence and absence of additional DNA elements contributing to specific virulence traits and also in the presence and absence of additional genetic information. (pasteur.fr)
Additionally, the presence of virulence-associated genes of E. coli was determined using a DNA "pathoarray" developed in our laboratory. (pasteur.fr)
For example, insertions led to the acquisition of antibiotic resistance genes in the virulence plasmid of the serovar Typhimurium, thus making it not only a virulence determinant, but also the source a multi-drug resistance phenotype (Herrero-Fresno et al. (springeropen.com)

In vivo enzymatic coupling using the general glycosylation pathway of Campylobacter jejuni in recombinant Escherichia coli has been suggested as a simpler method for producing conjugate vaccines. (biomedcentral.com)
Glycoconjugate synthesis, in particular expression of oligosaccharyltransferase PglB, strongly inhibited growth of E. coli cells after induction, making it necessary to separate biomass growth and recombinant protein expression phases. (biomedcentral.com)
The presented data demonstrate that glycosylated proteins can be produced in recombinant E. coli at a larger scale. (biomedcentral.com)
Recombinant Escherichia coli strains have been constructed by metabolic engineering strategies to produce inositol, but with a low yield. (biomedcentral.com)
The biosynthesis of inositol from glucose in recombinant E. coli was optimized by metabolic engineering strategies. (biomedcentral.com)

We developed an engineered E. coli strain and a fed-batch bioprocess to produce citramalate at concentrations in excess of 80 g l −1 in only 65 h. (microbiologyresearch.org)
In many laboratories, sorbitol-MacConkey medium is commonly used to screen for the slow sorbitol fermentation phenotype of the most common Shiga toxin-containing strain: O157:H7 ( 5 ), but this process does not address the pathogenic potential of the remaining sorbitol-positive E. coli . (cdc.gov)
7 In this article, we used our novel E. coli CRISPR λ-Red HR system to engineer an industrial E. coli strain with enhanced production of a valuable biomedical research compound known as Kdo2-lipid A. (sigmaaldrich.com)
We systematically investigated how a target E. coli strain can escape killing by episomally-encoded CRISPR-Cas9 antimicrobials. (dtu.dk)

If a model microbe like Escherichia coli with elaborate molecular genetic tools and resources can establish a mutualistic association with a host organism via experimental evolution, such a 'model experimental symbiotic system' will be extremely useful for understanding the evolutionary processes of symbiosis towards mutualism. (nature.com)
The most commonly used molecular approach for exploring microbial diversity is based on the PCR amplification and sequencing of genes encoding small subunit ribosomal RNA (16S rRNA) directly from environmental sources [ 16 ]. (biomedcentral.com)
Whole-genome SNP analysis applied directly to stool for genotyping Shiga toxin-producing Escherichia coli : An advanced molecular detection method for foodborne surveillance and outbreak tracking. (cdc.gov)

Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. (frontiersin.org)
Staehelin suggested that an animal could be protected against viral infection by inserting a gene encoding the Mx protein into that animal, preferably at the single cell embryo stage. (justia.com)
He taught that the gene of choice would be the one encoding the Mx protein that is normally found in the species of animal to be protected. (justia.com)
After induction glycoconjugates generally appeared later than unglycosylated carrier protein, suggesting that glycosylation was the rate-limiting step for synthesis of conjugate vaccines in E. coli . (biomedcentral.com)
E. coli is a most popular host for protein manufacturing as a consequence of its quick progress and the facility to particular proteins at very extreme ranges. (mygentaur.com)
E. coli is a most popular host for the look at of phage biology due to the detailed knowledge of its nucleic acid and protein biosynthetic pathways. (mygentaur.com)
The Escherichia coli chromosome or nucleoid consists of the genomic DNA, RNA, and protein. (mygentaur.com)

Quinolone resistance in Enterobacteriaceae results mainly from mutations in type II DNA topoisomerase genes and/or changes in the expression of outer membrane and efflux pumps. (biomedcentral.com)
Quinolone resistance among Enterobacteriaceae originally occurred due to chromosomal mutations in the quinolone-resistance-determining regions (QRDRs) of the gyrA and gyrB genes, which encode the gyrase A and B subunits, respectively and the parC and parE genes, which encode topoisomerase IV subunits (Hernández et al. (scielo.br)

Plasmid DNA of E. coli carrying a gene coding for ampicillin resistance remained intact for a 2-h period after ingestion, and was still able to transform recipient E. coli cells after this period. (jci.org)
A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. (justia.com)
Our invention relates to the introduction of the interferon gene of another animal species into a host animal, and to the production of a healthy transgenic animal with enhanced viral resistance which it can genetically transmit to its offspring. (justia.com)
The protocol involves insertion of an I- Sec I restriction site linked to an antibiotic resistance gene into the target plasmid using λ-Red mutagenesis, followed by the transformation with a temperature-sensitive auxiliary plasmid which carries I- Sec I nuclease expressed from a tetracycline-inducible promoter. (springeropen.com)
While the number of targets did not significantly affect efficiency of killing, it did reduce the emergence of chromosomal mutations conferring resistance. (dtu.dk)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)
This report presents the status of AMR in Africa by analysing the main types of resistance and the underlying genes where possible. (who.int)
Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)

Two different periplasmic carrier proteins, AcrA from C. jejuni and a toxoid form of Pseudomonas aeruginosa exotoxin were glycosylated with Shigella O antigens in E. coli . (biomedcentral.com)

No aneuploidy or chromosomal aberration is found in cultured human lymphocytes. (inchem.org)
We propose larger chromosomal alterations and aneuploidy as the basis of transformation in these organoids, consistent with the early appearance of chromosomal instability in CRC. (uni-muenchen.de)

abstract = "The genes coding for d-ribulose-1,5-bisphosphate carboxylase (RuBPCase) from an iron-oxidizing bacterium, Thiobacillus ferrooxidans, were cloned into an Escherichia coli plasmid, pUC18. (elsevierpure.com)

In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS SPI-6 and T6SS SPI-19 gene clusters in S . Dublin. (frontiersin.org)
It is generally believed that the proteins encoded in these genes are important for successful survival of the organism under the hostile conditions. (biomedcentral.com)
To examine whether the heat shock proteins affect the bactericidal action of FQs, we constructed E. coli strains with mutations in various heat shock genes and tested their susceptibility to FQs. (biomedcentral.com)
Figure 1(A) shows a profile of proteins in E. coli cells that were pulse-labeled after incubation for 10 min with different concentrations of LVFX. (biomedcentral.com)
In Escherichia coli , ribosomes must interact with translocons on the membrane for the proper integration of newly synthesized membrane proteins, cotranslationally. (rupress.org)
Recently, it has been recognized that membrane-bound ribosomes are crucial for biogenesis of integral membrane proteins in E. coli , thus renewing interest in ribosome targeting to and association with the membrane in this organism. (rupress.org)
1 Recombineering is a popular method that utilizes phage-derived proteins, the Lambda-Red (λ-Red) system, to increase the efficiency of recombination in E. coli . (sigmaaldrich.com)
The nucleoid varieties by condensation and purposeful affiliation of a single chromosomal DNA with the help of chromosomal architectural proteins and RNA molecules along with DNA supercoiling. (mygentaur.com)

Elita also leads genomics work investigating outbreaks and pathogenicity of other bacterial pathogens including E. coli , K. pneumoniae , methicillin-susceptible S. aureus , S. argenteus , S. pyogenes , S. pneumoniae and C. difficile . (imperial.ac.uk)

The flexibleness of E. coli to develop on chemically outlined media coupled with its intensive genetic toolbox make it a key system in look at of bacterial metabolic pathways. (mygentaur.com)

The Escherichia coli chromosome shows two main levels of global organization: macrodomains and replichores. (wikipedia.org)
4) The DNA inside the chromosome appears to have a condition-dependent 3-D development that is linked to gene expression so that the nucleoid construction and gene transcription are tightly interdependent, influencing each other reciprocally. (mygentaur.com)
The haploid spherical chromosome in E. coli consists of ~ 4.6 x 106 bp. (mygentaur.com)

Emergence and divergence of major lineages of Shiga-toxin-producing Escherichia coli in Australia. (cdc.gov)

Calva E. , Torres J. , Vazquez M. , Angeles V. , De La Vega H. , Ruiz-Palacios G. M. Campylobacter jejuni chromosomal sequences that hybridize to Vibrio cholerae and Escherichia coli LT enterotoxin genes. (microbiologyresearch.org)
Fauchere J. L. , Rosenau A. , Veron M. , Moyen E. N. , Richard S. , Pfister A. Association with HeLa cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. (microbiologyresearch.org)
Fauchere J.-L. , Kervella M. , Rosenau A. , Mohanna K. , Veron M. Adhesion to HeLa cells of Campylobacter jejuni and C coli outer membrane components. (microbiologyresearch.org)

However, conflicting results were obtained in assays for mitotic gene conversion and recombination. (inchem.org)
The chromosomal fragment of the target site of integration of modified F, is cloned by homologous recombination. (cam.ac.uk)
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template. (sigmaaldrich.com)
E. coli , one of the most commonly engineered bacteria, has an active homologous recombination (HR) system, so chromosomal integration can be achieved simply by donating DNAs with homology arms to recombine at a target site. (sigmaaldrich.com)

To further analyze the capacity of mononuclear leukocytes to degrade E. coli DNA, chromosomal and plasmid DNA was isolated from ingested bacteria and subjected to agarose gel-electrophoresis. (jci.org)
Until recently, CRISPR/Cas9-based gene editing methods 5 have been overlooked as a microbial gene editing tool because most bacteria lack endogenous DSB repair systems, rendering Cas9 nuclease cleavage toxic. (sigmaaldrich.com)

Thus, a single gene encoding a mesophilic variant of citramalate synthase from Methanococcus jannaschii, CimA3.7, was expressed in E. coli to convert acetyl-CoA and pyruvate to citramalate, and the ldhA and pflB genes were deleted. (microbiologyresearch.org)
2,3-BDO production from glycerol was dramatically enhanced by disruption of the pduC and ldhA genes in K. oxytoca M1 and 1,3-PDO-free 2,3-BDO production was achieved by using the double mutant and crude glycerol. (biomedcentral.com)

To solve this, we have developed a novel, dual-vector Cas9-mediated recombineering system that allows researchers to create scarless insertions, deletions, and replacements with incredibly high efficiency in E. coli . (sigmaaldrich.com)
That significant fractions of the bacterial genome could be involved in, or affected during, biofilm formation was shown in Escherichia coli in a genome-wide screen using random chromosomal insertions of a lacZ reporter gene fusion construct [ 5 ]. (biomedcentral.com)

In this study, we report an experimental system in which E. coli evolves into a bacterial mutualist that supports survival and reproduction of P. stali , thereby demonstrating that evolution of mutualism can proceed very easily and quickly via disruption of a global transcriptional regulator system. (nature.com)

Sääf AM, Halbleib JM, Chen X, Tsan Yuen S, Yi Leung S, Nelson WJ, Brown PO "Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal and Colon Cancer. (openwetware.org)

Escherichia coli is considered a model organism to analyze the processes involved in bacterial genome evolution, as the species comprises numerous pathogenic and commensal variants. (pasteur.fr)
The size of the DNA varies from 500,000 to numerous million base-pairs (bp) encoding from 500 to numerous thousand genes counting on the organism. (mygentaur.com)

There is ample evidence for the preservation of multiple gene copies due to selection for increased gene dosage in diverse organisms [ 20 ]. (biomedcentral.com)
To make this possible in Eshcrichia coli, which is one of the best organisms to understand cellular systems comprehensively based on the vast amount of accumulation of biological knowledge, we setup an easy and reliable system for construction double knockout strains by conjugation and for analysis of their growth effects. (cam.ac.uk)

Halbleib JM, Sääf AM, Brown PO, Nelson WJ "Transcriptional Modulation of Genes Encoding Structural Characteristics of Differentiating Enterocytes During Development of a Polarized Epithelium In Vitro. (openwetware.org)
Buess M, Nuyten DS, Hastie T, Nielsen T, Pesich R, Brown PO "Characterization of heterotypic interaction effects in vitro to deconvolute global gene expression profiles in cancer. (openwetware.org)

Gene and genome duplications are the primary source of new genes and have played a pivotal role in the evolution of genomic and organismal complexity [ 1 - 4 ]. (biomedcentral.com)
Recent genomic and proteomic studies have identified many of the genes and gene products differentially expressed during biofilm formation, revealing the complexity of this developmental process. (biomedcentral.com)

Membrane-bound ribosomes in E. coli were extensively studied over 20 years ago. (rupress.org)

Here, we demonstrate that 1 kb of flanking, non-coding synthetic sequences with a low frequency of guanosine and cytosine (GC) can dramatically reduce reporter expression compared to neutral and high GC-content flanks in E. coli . (biorxiv.org)

Targeting lacZ in wild-type E. coli allows simple blue-white screening to identify edited mutant strains. (sigmaaldrich.com)
cells transformed with a lacZ-targeting gRNA and a donor DNA template are successfully edited at the lacZ locus, knocking out function of this gene and yielding white colonies. (sigmaaldrich.com)

Preferably, the human beta interferon gene is introduced into the cells of a mouse. (justia.com)
Apparently, Kawade had not determined whether the chimeric gene was actually expressed in the mice, but had already been able to express it in cultured cells induced by Cd.sup.2+ or by heat. (justia.com)
This plasmid can replicate in cells supplying pir gene product. (cam.ac.uk)
Organoids recovered from short-term infection with pks+ E. coli show characteristics of CRC cells, e.g., enhanced proliferation, Wnt-independence, and impaired differentiation. (uni-muenchen.de)
E. coli is a most popular host for gene cloning due to the extreme effectivity of introduction of DNA molecules into cells. (mygentaur.com)
The chromosomal DNA is present in cells in a extraordinarily condensed, organized sort known as nucleoid (nucleus-like), which is not encased by a nuclear membrane as in eukaryotic cells. (mygentaur.com)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)

3a) Arber, W. &Dussoix, D. (1962) Host specificity of DNA produced by Escherichia coli. (odoo.com)

The recent Encyclopedia of studies in the 1950s and 1960s in Escherichia coli and then by DNA Elements (ENCODE) project showed binding of carcinogens to nu- Szybalski (1958) , who tested more indicates that at least 80% of the cleic acids ( Wiest and Heidelberger, than 400 compounds in E. coli . (who.int)

The increased susceptibility of the lon mutant was corroborated by experiments in which the gene encoding the cell division inhibitor, SulA, was subsequently disrupted. (biomedcentral.com)

Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. (igem.org)

We established a ribozyme reporter system in Escherichia coli in which a significant reduction of reporter activity is manifest when an active ribozyme sequence is fused to the reporter gene and the expression of a foreign Bacillus subtilis RNaseJ1 5′ exonuclease is induced from a chromosomally-integrated gene in the same cell. (biomedcentral.com)
With so many genes involved, it is perhaps not surprising that biofilm formation is regarded as a developmental process (Figure 1 ), not unlike that observed in the formation of fruiting bodies containing spores by the soil bacterium Myxococcus xanthus and sporulation in Bacillus subtilis [ 6 ]. (biomedcentral.com)
Why is Bacillus subtilis respiratory Complex II not assembled when produced in Escherichia coli? (lu.se)

Alternatively, cell-based reporter gene assays can be used to validate ribozyme function. (biomedcentral.com)
In these assays, the ribozyme sequence is fused to a reporter gene which modulates its activity when expressed as RNA. (biomedcentral.com)

In contrast, natural selection for increased gene expression may represent an important mechanism by which duplicate gene copies are maintained in populations [ 14 ]. (biomedcentral.com)
The gene encodes the interferon of a different animal species and therefore its expression product is less toxic to the host at an embryonic, fetal, neonatal or junvenile stage of development. (justia.com)
It is well known that expression of certain bacterial genes responds rapidly to such stimuli as exposure to toxic chemicals and physical agents. (biomedcentral.com)
however, the glmS ribozyme is the only known example of a natural ribozyme that functions to regulate gene expression in a ligand-dependent manner by requiring glucosamine-6-phosphate (GlcN6P) as a cofactor [ 13 ]. (biomedcentral.com)
Plasmid-based expression systems for IPS and IMP were optimized, and expression of the gene zwf was regulated to enhance the stoichiometric yield of inositol. (biomedcentral.com)
But a comparison of the differentially expressed gene sets identified in several recent DNA microarray studies [ 7 - 10 ] reveals that no common expression pattern for biofilms has yet emerged. (biomedcentral.com)
One explanation for these apparent discrepancies is that DNA microarrays provide a sensitive but transient snapshot of gene expression and that gene expression does not necessarily directly correlate with phenotype. (biomedcentral.com)
Rubins KH, Hensley LE, Wahl-Jensen V, Daddario Dicaprio KM, Young H, Reed DS, Jahrling PB, Brown PO, Relman DA, Geisbert TW "The temporal program of peripheral blood gene expression in the response of non-human primates to Ebola hemorrhagic fever. (openwetware.org)
Rinn JL, Bondre C, Gladstone HB, Brown PO, Chang HY "Anatomic demarcation by positional variation in fibroblast gene expression programs. (openwetware.org)
Palmer C, Diehn M, Alizadeh AA, Brown PO "Cell-type specific gene expression profiles of leukocytes in human peripheral blood. (openwetware.org)
Sood R, Zehnder JL, Druzin ML, Brown PO "Gene expression patterns in human placenta. (openwetware.org)
iv) how among the many DNA structural options have an effect on gene expression, using the gram-negative bacterium Escherichia coli as a model system. (mygentaur.com)

The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)

Anatomy10

Organisms29

Diseases6

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes85

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Salmonella8

Isolates23

Enterohemorrhagic Esc1

Aberrations4

Mutations in the genes2

Induce2

Mammalian2

Mutation3

Plasmid-mediated quinolone resistance2

Adherent-invasive1

Plasmids3

Genomes1

Deletion3

Analyses4

Replication2

Virulence4

Recombinant5

Strain4

Molecular3

Protein7

Topoisomerase2

Resistance8

Antigens1

Aneuploidy2

Abstract1

Proteins8

Genomics1

Metabolic1

Chromosome3

Shiga1

Campylobacter3

Recombination4

Bacteria2

LdhA2

Insertions2

Disruption1

Intestinal1

Organism2

Organisms2

Vitro2

Complexity2

Membrane-bound1

Sequences1

LacZ2

Cells7

Specificity1

Encode1

Susceptibility1

Promoter1

Bacillus3

Assays2

Expression12

Mutant1