This process is analogous to hydrolysis of X-gal by Beta-galactosidase to produce blue cells as is commonly practiced in bacterial reporter gene assays. (wikipedia.org)
After removal of the methyl ester group by unspecific hydrolysis, the product is cleaved by beta-glucuronidase in liquid media and yields an intense pink color. (rapidmicromethods.com)
β-galactosidase , also called lactase , beta-gal or β-gal , is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond . (wikidoc.org)
Recombinant1
High purity recombinant β-Glucuronidase ( Escherichia coli ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
Enumeration6
BDS EN ISO 16649-2: 2014, Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide. (ijfsab.com)
BDS EN ISO 21527-2: 2011, Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and moulds - Part 2: Colony count technique in products with water activity less than or equal to 0,95. (ijfsab.com)
BDS EN ISO 4833-1: 2013, Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique. (ijfsab.com)
BDS EN ISO 6579-1: 2017, Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. (ijfsab.com)
BDS EN ISO 6888-1:2022, Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Method using Baird-Parker agar medium (ISO 6888-1:2021). (ijfsab.com)
3M™ Petrifilm™ E. coli/Coliform Count (EC) Plates contain Violet Red Bile (VRB) nutrients, a cold-water-soluble gelling agent, an indicator of glucuronidase activity, and an indicator that facilitates colony enumeration. (thomassci.com)
Molecular3
The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. (wikipedia.org)
Diez, T. and Cabezas, J.A. Properties of two molecular forms of β-glucuronidase from the mollusc Littorina littorea L. (enzyme-database.org)
Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo- beta- (1 ®6)-galactanase gene. (edu.bd)
Substrates3
There are different possible glucuronide that can be used as substrates for the β-glucuronidase, depending on the type of detection needed (histochemical, spectrophotometrical, fluorimetrical). (wikipedia.org)
For other types of detection, common substrates are p-nitrophenyl β-D-glucuronide for the spectrophotometric assay and 4-methylumbelliferyl-beta-D-glucuronide (MUG) for the fluorimetric assay. (wikipedia.org)
It can be reliably detected with growth media that contain substrates for beta-glucuronidase, an enzyme that occurs almost exclusively in E. coli. (rapidmicromethods.com)
Species2
Some major bacterial producers of fecal β-G include Bifidobacterium, Lactobacillus, Escherichia coli, Clostridium, Bacteroides fragilis and other Bacteroides species, Ruminococcus gnavus, and species that belong to the genera Staphylococcus and Eubacterium. (doctorsdata.com)
The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)
Coliform1
SimPlate for Total Coliform & E. coli Color Indicator (CEc-CI) method is used for the detection and quantification of the Total Coliform and E. coli populations. (thomassci.com)
Enzymes1
Therefore, the characterization of the endo-1,4-beta-xylanase, encoded by um06350.1, is a further step towards the biotechnological application of U . maydis and its enzymes. (biomedcentral.com)
Intestinal2
Higher levels of beta-glucuronidase may be associated with an imbalanced intestinal microbiota profile, as well as higher circulating estrogens and lower fecal excretion of estrogens in premenopausal women. (doctorsdata.com)
β -glucuronidase is produced by the intestinal epithelium and certain intestinal bacteria. (doctorsdata.com)
Detection8
A Sensitive Voltammetric Biosensor for Escherichia Coli Detection Using an Electroactive Substrate for ß -D-Glucuronidase. (donau-uni.ac.at)
8-hydroxyquinoline-glucuronide sodium salt used as electroactive substrate for a sensitive voltammetric detection of Escherichia coli in water samples. (donau-uni.ac.at)
An automated, Robotic Biosensor for the Electrochemical Detection of E. Coli in Water. (donau-uni.ac.at)
A fluorescence based sensor system for automated detection of E. coli in water. (donau-uni.ac.at)
Development of an Automated Biosensor for Rapid Detection and Quantification of E. coli in Water. (donau-uni.ac.at)
Development of a biosensor for the amperometric phage-mediated detection of Escherichia coli in aqueous samples. (donau-uni.ac.at)
Today 4-Methylumbelliferyl beta-D-glucuronide (MUG) is recommended in international guidelines as a fluorogenic substrate for E. coli detection. (rapidmicromethods.com)
In GUS, the reporter is the β-glucuronidase enzyme from Escherichia coli, capable of transforming non-fluorescent substates into fluorescent, thereby allowing detection. (collectf.org)
Clonal1
The isolates appear clonally unrelated with MLST, revealing a diversity of clonal types among the Klebsiella pneumoniae and Escherichia coli isolates. (who.int)
Assays2
In standard assays bacteria culture media are supplemented with MUG and incubated at 37°C. In the presence of E. coli a blue fluorescence can be detected under UV light after 24 hours. (rapidmicromethods.com)
These findings suggest the usage of RUG™ in place of MUG for the development of fast fluorescence E. coli assays. (rapidmicromethods.com)
Shiga3
Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. (cdc.gov)
Shiga toxin (Stx)-producing Escherichia coli (STEC) with the serotype O157:H7 is characterized by the possession of stx gene(s), the locus of enterocyte effacement (LEE) -encoded type 3 secretion system (T3SS), and a large virulence plasmid (pO157) that encodes enterohemolysin and other virulence factors, such as catalase-peroxidase KatP, a type II secretion system, and the protease EspP ( 1 ). (cdc.gov)
Culture-independent diagnostic tests (CIDTs) are increasingly used for clinical diagnosis of gastrointestinal diseases such as salmonellosis, Shiga toxin-producing E. coli disease, and shigellosis because of their speed, convenience, and generally high-performance characteristics. (cdc.gov)
Gene2
In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikidoc.org)
Very similar in spirit to the standard beta-galactosidase reporter, in this assay genes coding for a protein of interest are fused with the phoA gene encoding alkaline phosphatase. (collectf.org)
Produces1
Escherichia coli produces the enzyme β-D-glucuronidase, which hydrolyzes β-D-glucopyranosid-uronic derivatives to aglycons and D-glucuronic acid. (microbeonline.com)
Strains1
Non-sorbitol-fermenting (NSF) and β-glucuronidase-negative (GN) STEC O157:H7 (hereafter referred to as typical STEC O157:H7), the major clone among currently circulating STEC O157:H7 strains, frequently causes large outbreaks of severe enteric infections, including diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. (cdc.gov)
Clinical1
In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae ( Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae ) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. (who.int)
Fluorescent1
Biosynth recently launched RUG™, a new substrate for beta-glucuronidase that is based on Resorufin - one of the most intensely fluorescent materials known today. (rapidmicromethods.com)
Indicator2
Escherichia coli is the most important indicator organism for fecal contamination of drinking water, food and beverages. (rapidmicromethods.com)
RUG™ (Resorufin-beta-D-glucuronic acid methyl ester) is a highly sensitive chromogenic and fluorogenic indicator for E. coli. (rapidmicromethods.com)
Iptg1
[3] Beta-gal is inhibited by L-ribose , non-competitive inhibitor iodine , and competitive inhibitors phenylthyl thio-beta-D-galactoside (PETG), D-galactonolactone, isopropyl thio-beta-D-galactoside (IPTG) , and galactose. (wikidoc.org)
Protein1
Certain organisms such as Streptococcus agalactiae (Group B streptococci), produce a diffusible extracellular protein (CAMP) factor that acts synergistically with the beta-lysin of Staphylococcus aureus and causes enhanced lysis of RBCs. (microbeonline.com)
Activity2
An organism is suitable for a GUS assay if it lacks naturally occurring β-glucuronidase activity or if the activity is very low (background activity). (wikipedia.org)
the apigenin and luteolin like flavonoids were generally inhibit basophil histamine release and neutrophils beta glucuronidase release, and finally shows in-vivo antiallergic activity. (scind.org)
Product2
Quercetin-7-O-beta-D-glucopyranoside, a natural product isolated and purified from the herbs of Rostellularia procumbens with antibacterial, antioxidative and anti-inflammatory activities, inhibits overexpression of cyclooxygenase-2 and granulocyte macrophage-colony-stimulating factor. (csnpharm.cn)
Oroxylin A 7-O-beta-D-glucuronide, a natural product isolated and purified from the roots of Scutellaria regeliana Nakai. (csnpharm.cn)
Sequences1
Beta-galactosidase has many homologues based on similar sequences. (wikidoc.org)
Cells1
A putative endo-1,4-beta-xylanase, encoded by um06350.1, was identified in the supernatant of xylan-grown cells. (biomedcentral.com)
Function2
Beta-glucuronidase can function through a wide range of pH values, and is fairly resistant to thermal inactivation. (wikipedia.org)
Many adult humans lack the lactase enzyme, which has the same function of beta-gal, so they are not able to properly digest dairy products. (wikidoc.org)
Potential1
Beta-glucuronidase (ßG) is a potential biomarker for cancer diagnosis and prodrug therapy. (bvsalud.org)
Human1
Beta-galactose is used in such dairy products as yogurt, sour cream, and some cheeses which are treated with the enzyme to break down any lactose before human consumption. (wikidoc.org)