• For these reasons, on the industrial scale erythritol has been produced from glucose in biotechnological processes by osmophilic yeast such as Trichosporonoides sp. (biomedcentral.com)
  • To date, this is the first reported study employing metabolic engineering of the native gene involved in the erythritol pathway to result in a high titer of the polyol. (biomedcentral.com)
  • Erythritol is a natural four-carbon polyol which occurs in wine, honey and fermented food. (biomedcentral.com)
  • Erythritol is an important nutrient for several α-2 Proteobacteria, including N2-fixing plant endosymbionts and Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues. (uclouvain.be)
  • This is the first description to our knowledge of a route feeding carbohydrate metabolism exclusively via d-erythrose 4-phosphate, a pathway that may provide clues to the preferential metabolism of erythritol by Brucella and its role in pathogenicity. (uclouvain.be)
  • The amino acid sequence of the Y. lipolytica ER showed a high degree of similarity to the previously described erythrose reductases from known erythritol producers, such as Candida magnoliae and Moniliella megachiliensis . (biomedcentral.com)
  • Erythritol metabolism involves phosphorylation to l-erythritol-4-phosphate by the kinase EryA and oxidation of the latter to l-3-tetrulose 4-phosphate by the dehydrogenase EryB. (uclouvain.be)
  • renamed EryI), a pathway fully consistent with the isotopomer distribution of the erythrose-4-phosphate-derived amino acids phenylalanine and tyrosine obtained from bacteria grown on (13)C-labeled erythritol. (uclouvain.be)
  • The addition of Zn 2+ ions at 0.25 mM had a positive effect on the activity of erythrose reductase from Y. lipolytica , as well as on the erythritol production. (biomedcentral.com)
  • Here, we found that the gene overexpression results in an enhanced titer of erythritol of 44.44 g/L (20% over the control), a yield of 0.44 g/g and productivity of 0.77 g/L/h. (biomedcentral.com)
  • Accordingly, growth on erythritol as the sole C source should require aldolase and fructose-1,6-bisphosphatase to produce essential hexose-6-monophosphate. (uclouvain.be)
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