Primary antibodiesERK1 and ERK2MAPK1Polyclonal antibodyReactivityImmunogenKinaseWestern BlotSpecificityAbcamTyr187IsotypeLysatesRabbit monoclonalMonoclonal antibodiesThermo Fisher SciAntigenPhosphorylationCatalogQuality antibodiesDetectsDifferent antibodiesPeptideRecombinantDatasheetCross-reactAssaysImmunoglobulinDilutionsConjugationImmunoblotsIncubationSubstrateImmunocytochemistryRecognizePeptidesRecognizesLaboratoriesMouseVariantsA431ActinMiceActivationCellsOptimalConcentration
Primary antibodies4
- Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
- Anti-ZKSCAN5 (SAB4501021), anti-VEGFC (ab83905), and anti-LYVE1 (ab10278) primary antibodies were used at 1:100, 1:100, and 1:50 dilutions, respectively. (bms-911543.com)
- Source of antibodies For the techniques used in this review, the next primary antibodies had been utilized, full report MOAB two, IgG2b, 6E10 anti Ab residues three 8, mouse IgG1, 0. (liverxreceptor.com)
- Abcepta is a leading manufacturer of primary antibodies with more than fifteen years of experience. (abcepta.com)
ERK1 and ERK26
- Two similar protein kinases with 85% sequence identity were originally called ERK1 and ERK2. (wikipedia.org)
- Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). (lsbio.com)
- ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. (assaysolution.com)
- ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. (assaysolution.com)
- Description: IC50: inhibits the phosphorylation of ERK1 and ERK2 at 25-50 ?MAG-126 is an ERK1 and ERK2 inhibitor. (english-nature.org)
- Consistent with their high activity, ERK1 and ERK2 were doubly phosphorylated on Tyr and Thr, were recognized by an antibody specific to the doubly phosphorylated forms, and were inactivated by either phosphoprotein phosphatase 2A or phosphotyrosine phosphatase type 1. (elsevierpure.com)
MAPK110
- Mitogen-activated protein kinase 1 (MAPK1) is also known as extracellular signal-regulated kinase 2 (ERK2). (wikipedia.org)
- Conditional deletion of Mapk1 in B cells showed a role for MAPK1 in T-cell-dependent antibody production. (wikipedia.org)
- The following product was used in this experiment: MAPK1 Monoclonal Antibody (OTI4C2), TrueMAB™ from Thermo Fisher Scientific, catalog # CF500485. (thermofisher.com)
- MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. (thermofisher.com)
- Affinity and binding kinetics of CPTC-MAPK1-1 antibody and full-length MAPK1 recombinant protein were measured using biolayer interferometry. (cancer.gov)
- CPTC-MAPK1-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM. (cancer.gov)
- Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- MAPK1-1 antibody. (cancer.gov)
- Indirect ELISA using CPTC-MAPK1-1 antibody as primary rabbit antibody against full length recombinant MAPK1 protein. (cancer.gov)
- MAPK1 recombinant protein was coated on the plate and detected using goat anti-rabbit antibody and TMB. (cancer.gov)
- Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. (arigobio.com)
Polyclonal antibody1
- ERK1 antibody LS-C16352 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from human. (lsbio.com)
Reactivity2
- Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby. (reageno.com)
- Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. (reageno.com)
Immunogen3
- The exact immunogen sequence used to generate this antibody is proprietary information. (abcam.com)
- If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (abcam.com)
- Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abcepta.com)
Kinase5
- Genistein, a specific tyrosine kinase inhibitor, prevented the phosphorylation of ERK2 by BK. (nih.gov)
- Curcumin-induced paraptosis has been reported to be positively associated with ERK2 and JNK (c-jun N-terminal kinase-1) activation [ 16 ]. (hindawi.com)
- MAP3K8 is one of the MAP kinase kinase (MAPKK) kinases that regulates the ERK1/ERK2 pathway in response to IL-1, facilitating IL-8 and MIP-1 beta production. (bio-techne.com)
- A two-plasmid bacterial expression system was employed to express active forms of the following MEK and MAP kinase family members: ERK1, ERK2, α-SAPK, and p38 and their upstream activators, MEK1, -2, -3, and -4. (elsevierpure.com)
- Likewise, activated p38 and α-stress-activated protein kinase could also be inactivated by either phosphatase, and α-stress- activated protein kinase was recognized by an antibody specific to the doubly phosphorylated forms. (elsevierpure.com)
Western Blot3
- Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. (novusbio.com)
- Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. (novusbio.com)
- Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. (novusbio.com)
Specificity1
- This assay has high sensitivity and excellent specificity for detection of Porcine Total ERK2. (assaysolution.com)
Abcam1
- Abcam: antibodies, proteins, kits. (abcam.com)
Tyr1871
- Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. (assaysolution.com)
Isotype2
- Humoral memory is characterized by recall immune responses, which are more rapid than the primary response, and by the production of higher serum titers of antigen-specific antibodies, mostly of the IgG isotype. (osaka-u.ac.jp)
- Membranes have been incubated with a hundred ng ml of MOAB 2 or IgG2b isotype matched management and then rabbit anti mouse antibody conjugated with HRP and visualized with ECL substrate. (liverxreceptor.com)
Lysates5
- B ) Lysates of various mouse tissues (15 µg each) indicated at the top were analyzed by Phos-tag PAGE (upper panel), SDS-PAGE (lower panel), and immunoblotting with anti-Pex14 antibody. (elifesciences.org)
- Cell lysates were analyzed as in A with antibodies to Pex14, phosphorylated Pex14 at Ser232 (Pex14-pS232), and lactate dehydrogenase (LDH). (elifesciences.org)
- After the treatment with mock or 0.2 mM H 2 O 2 for 0.5 hr and 1 hr, Fao cells were incubated with vehicle (-) and λ-phosphatase (+) as in A. The cell lysates were analyzed by Phos-tag PAGE (upper panels), SDS-PAGE (lower panels), and immunoblotting with anti-Pex14 antibody. (elifesciences.org)
- Cell lysates were analyzed by SDS-PAGE (upper panel), Phos-tag PAGE (lower panels), and immunoblotting with indicated antibodies. (elifesciences.org)
- HeLa whole cell lysates were subjected to Western blotting with an anti-phospho-STAT3 antibody. (scienceimaging.se)
Rabbit monoclonal1
- Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
Monoclonal antibodies1
- Monoclonal antibodies have been then purified to homogeneity working with regular approaches just before storage at 1 mg ml or 0. (liverxreceptor.com)
Thermo Fisher Sci1
- The following product was used in this experiment: SOX9 Monoclonal Antibody (1B11) from Thermo Fisher Scientific, catalog # MA5-17177, RRID AB_2538648. (thermofisher.com)
Antigen5
- One of our phospho-site-selective antibodies was generated using a triply phosphorylated peptide as the antigen. (utmb.edu)
- AdvanBlock™-Chemi is a novel blocking solution optimized to enhance specific antibody-antigen interactions for the best results in chemiluminescent Western Blots. (scienceimaging.se)
- Non-specific binding caused by low quality antibodies is reduced while signal from the specific antibody-antigen complex is stabilized and enhanced. (scienceimaging.se)
- In the GC, the combined processes of somatic hypermutation and selection based on the affinity of the BCR for the antigen, are responsible for the generation of high-affinity antibody variants which ultimately differentiate into long-lived plasma cells or long-lived memory B cells. (osaka-u.ac.jp)
- Preliminary clonal assortment was performed by antigen antibody blotting. (liverxreceptor.com)
Phosphorylation2
- For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. (abcepta.com)
- This complex behavioral profile was accompanied by decreases in ERK2 mRNA and protein phosphorylation within the VTA, while stress alone resulted in opposite neurobiological effects. (jneurosci.org)
Catalog2
- Free shipping on catalog antibody and protein orders over $999. (abclonal.com)
- PVDF Membrane was probed with 1 µg/mL of Human MAP3K8/Tpl2/COT Monoclonal Antibody (Catalog # MAB4586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). (bio-techne.com)
Quality antibodies1
- We supply quality antibodies and related products drug discovery and academic laboratories worldwide. (abcepta.com)
Detects3
- This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain. (novusbio.com)
- HSF1 Antibody detects endogenous levels of total HSF1. (affbiotech.com)
- C-RAF Antibody detects endogenous levels of total C-RAF. (affbiotech.cn)
Different antibodies2
- Twenty different antibodies were used in Western blotting using NFDM or AdvanBlock™-chemi. (scienceimaging.se)
- B lymphocytes can produce an unlimited number of different antibodies against a diverse array of foreign bodies by utilizing random rearrangement of gene segments encoding antibodies (immunoglobulin genes). (osaka-u.ac.jp)
Peptide4
- This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP. (novusbio.com)
- These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)
- Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
- The majority of Abcepta antibodies are produced using peptide immunogens. (abcepta.com)
Recombinant2
- Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. (abcam.com)
- Sandwich ELISA: ZNF398 Antibody (4G3) [H00057541-M10] - Detection limit for recombinant GST tagged ZNF398 is 0.3 ng/ml as a capture antibody. (novusbio.com)
Datasheet1
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
Cross-react1
- This particular antibody might cross react with speacies outside of the listed ones. (reageno.com)
Assays1
- For assays using obstructing antibody, cells had been pretreated with 2.5 g/ml AIIB2 or control rat IgG for 48 h in the current presence of DMSO or AZD6244 in the concentrations in the above list. (bibf1120.com)
Immunoglobulin1
- Ige Antibody Laboratories manufactures the immunoglobulin ige = 126 reagents distributed by Genprice. (english-nature.org)
Dilutions1
- Please Note: Optimal dilutions of this antibody should be experimentally determined. (bio-techne.com)
Conjugation1
- To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (thermofisher.com)
Immunoblots2
- Immunoblots using antibodies specific for ERK or tyrosine-phosphorylated ERK revealed a shifting of p42 ERK2 to a higher molecular weight that correlated temporally with an increase in tyrosine-phosphorylated ERK2. (nih.gov)
- Antibodies and Immunoblots. (bibf1120.com)
Incubation2
- This all-in-one blocking and antibody incubation solution is designed to improve sensitivity and decrease overall background. (scienceimaging.se)
- Immediately after a 3 × 5 min washing step with 1x PBS, incubation using the second antibody coupled to Alexa488, Alexa568 or Alexa647 for 1 h followed. (liverxreceptor.com)
Substrate1
- We determined that the Kv4.2 C-terminal cytoplasmic domain is an effective ERK2 substrate, and that it is phosphorylated at three sites: Thr 602 , Thr 607 , and Ser 616 . (utmb.edu)
Immunocytochemistry1
- Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. (novusbio.com)
Recognize3
- We used this information to develop antibodies that recognize Kv4.2 phosphorylated by ERK2. (utmb.edu)
- The core function of this immune system is executed by B lymphocytes, which recognize foreign bodies and eliminate them by producing antibodies. (osaka-u.ac.jp)
- It is our biggest issue to clarify what sorts of mechanisms make B lymphocytes to differentiate and proliferate, to select cells that recognize foreign bodies (good cells) or our body itself (bad cells), to eliminate bad cells and maintain good cells and to differentiate good cells into plasma cells, thereby leading to production of only good antibodies. (osaka-u.ac.jp)
Peptides2
- Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. (affbiotech.com)
- These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications. (abcepta.com)
Recognizes1
- We determined that this antibody recognizes ERK-phosphorylated Kv4.2 in COS-7 cells transfected with Kv4.2 and native ERK-phosphorylated Kv4.2 in the rat hippocampus. (utmb.edu)
Laboratories1
- Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. (abcepta.com)
Mouse1
- The ERK2-DBP domain opposes pathogenesis of a mouse JAK2V617F-driven myeloproliferative neoplasm. (nih.gov)
Variants1
- Little effect of H 2 O 2 treatment on Pex14 level and evaluation of Pex14 variants with anti-Pex14-pS232 antibody. (elifesciences.org)
A4311
- A) Averaged outcomes of reverse-phase antibody array evaluation of 46 signalling protein in A431 cells stably expressing shDUSP6, shDUSP7, or non-silencing shRNA control. (synanet2020.com)
Actin1
- Samples were separated by Phos-tag PAGE (top panel) and SDS-PAGE (middle and bottom panels) and analyzed by immunoblotting with antibodies to Pex14 and actin, a loading control. (elifesciences.org)
Mice1
- Metabolic Remodeling with Hepatosteatosis Induced Vascular Oxidative Stress in Hepatic ERK2 Deficiency Mice with High Fat Diets. (nih.gov)
Activation2
- Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation. (cusabio.com)
- Additionally, collagen I can induce DDR2/ERK2/SNAIL1 signaling activation in HCC cells. (biomedcentral.com)
Cells2
- 5% cold fish gelatine and 0,1% ovalbumin 1x PBS for thirty min at RT plus the cells have been washed again 3 × 5 min with 1x PBS at RT, followed by the major antibody at four C overnight. (liverxreceptor.com)
- MDA-MB-231 or Amount159PT cells had been invert transfected in 35-mm glass-bottom tradition dishes (MatTek Company, Ashland, MA) or treated with 2.5 g/ml monoclonal antibody AIIB2 (Developmental Research Hybridoma Bank, University of Iowa) or normal rat IgG missing azide (Santa Cruz Biotechnology, Santa Cruz, CA). Cells had been treated with 500 nM (MDA-MB-231) or 250 nM (Amount159PT) AZD6244 or DMSO automobile control. (bibf1120.com)
Optimal1
- For optimal long term storage, the antibody should be kept at -20 degrees Celsius. (reageno.com)
Concentration1
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. (thermofisher.com)