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  • protein
  • ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. (thermofisher.com)
  • In vertebrates the mitogen-induced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras. (rockland-inc.com)
  • The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. (rockland-inc.com)
  • p42 MAP Kinase (ERK2) is a ubiquitous protein kinase target for Ras and Raf. (rockland-inc.com)
  • This antiserum is directed against human p42 MAP Kinase (ERK2) protein and is useful in determining its presence by immunoblotting. (rockland-inc.com)
  • Erk2 is also known as microtubule-associated protein-2 kinase MAP kinase 2, p42-MAPK, or EGF receptor T669 (ERT1). (thermofisher.com)
  • Erk2, a serine/threonine kinase, phosphorylates microtubule associated protein-2 and myelin basic protein. (thermofisher.com)
  • Recombinant protein encompassing a sequence within the center region of human ERK2. (genetex.com)
  • In this review we compiled data on ERK1 vs. ERK2 gene structures, protein sequences, expression levels, structural and molecular mechanisms of activation and substrate recognition. (frontiersin.org)
  • Furthermore, for extracellular signal-regulated kinase 2 (ERK2) the protein-peptide docking surface is comprised of a small hydrophobic surface patch that is often engaged in the crystal packing of apo ERK2 crystals. (iucr.org)
  • Here, a rational surface-engineering approach is presented that involves mutating protein surface residues that are distant from the peptide-binding ERK2 docking groove to alanines. (iucr.org)
  • Unfortunately, ERK2 readily crystallized in the apo form and we only managed to grow protein-peptide cocrystals with the docking peptide from MNK1 (pepMNK1), which is an ERK2 substrate. (iucr.org)
  • Analysis of crystal-packing contacts subsequently revealed that the protein-peptide binding surface of ERK2 WT was blocked by a symmetry-related kinase molecule in all apo crystals, while ERK2-pepMNK1 crystals could `luckily' form because this peptide mediated a different type of crystal packing. (iucr.org)
  • Mapk
  • Western blot analysis of extracts from various cell lines, serum-starved overnight and untreated (-) or treated with U0126 #9903, hEGF #8916, or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (upper) or p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696 (lower). (cellsignal.com)
  • right), using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red). (cellsignal.com)
  • Flow cytometric analysis of Jurkat cells, treated with U0126 #9903 (10 μM, 2 hr) (blue) or TPA #4174 (200 nM, 15 min) (green), using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb. (cellsignal.com)
  • Reactivity
  • Reactivity has been confirmed by western blot analysis of extracts derived from human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain, and recombinant ERK2. (thermofisher.com)
  • HeLa
  • The following cell lines have been assayed by immunoblot and were found to be positive for p42 MAP Kinase (ERK2) using this reagent: U937, HeLa, NIH-3T3, RAW 264.7, LNCaP and HEK whole cell lysates. (rockland-inc.com)
  • Mek1
  • Here we show, using a synthetic small molecule MEK1/2 inhibitor (U0126) and RNA silencing of ERK1 and 2, comparatively, that ERK2 is critical to transformation and homeostasis of human epithelioid malignant mesotheliomas (MMs), asbestos-induced tumors with a poor prognosis. (pubmedcentralcanada.ca)
  • siRNA
  • Using a chicken model, we have examined the effects of a small interfering RNA (siRNA) targeting ERK2 delivered by a lentiviral system on tendon adhesion formation with an adhesion scoring system, histological assessment, and biomechanical evaluation. (mdpi.com)
  • Moreover, no statistically significant reduction in breaking force was detected between the ERK2 siRNA group and the control group. (mdpi.com)
  • immediate early
  • A stimulation induced PARP1 binding to phosphorylated Erk2 in the chromatin of cerebral neurons caused Erk-induced PARP1 activation, rendering transcription factors and promoters of immediate early genes (IEG) accessible to PARP1-bound phosphorylated Erk2. (jefferson.edu)
  • ERKs
  • To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). (thermofisher.com)
  • western
  • Expect a predominant band approximately 42 kDa in size corresponding to p42 MAP Kinase (ERK2) by western blotting in the appropriate cell lysate or extract. (rockland-inc.com)
  • Western blot analysis of ERK2 on rat pituitary lysate. (antibodies-online.com)