EpidermalHER2Signaling pathwayEGFROverexpressionGeneAntibodyProteinPrognosisLigand-boundAntibodiesStratified by hormone receptorPhosphorylationKinaseTargetsExtracellular domainResiduesTumorCognate ligandsInhibitorActivationCancersNode-positive breast cancerImmunohistochemicalConferHumanFamilyAmplificationTherapeuticBreast cancerPrognosticEssentialAmino acidsAssayLung
Epidermal17
- ERBB2 is a protein that is also known as HER2 (human epidermal growth factor receptor 2). (antibodyguide.com)
- It is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. (antibodyguide.com)
- Patients who have residual invasive breast cancer after receiving neoadjuvant chemotherapy plus human epidermal growth factor receptor 2 (HER2)-targeted therapy have a worse prognosis than those who have no residual cancer. (nih.gov)
- Expression analyses of epidermal growth factor receptor and HER-2/neu: no advantage of prediction of recurrence or survival in breast cancer patients. (thieme-connect.de)
- The ErbB/HER family of receptor tyrosine kinases (RTKs) consists of four family members: the epidermal growth factor receptor (EGFR/ErbB1), HER2 (ErbB2/Neu), HER3 (ErbB3), and HER4 (ErbB4). (plos.org)
- It selectively inhibits erbB1 (epidermal growth factor receptor), erbB2, erbB3, and erbB4 without blocking tyrosine kinase activity of many other receptors such as platelet-derived growth factor receptor, fibroblast growth factor receptor, and insulin receptor. (lclabs.com)
- Recently, we isolated a novel negative regulator of epidermal growth factor receptor (EGFR), termed EGFR-related protein (ERRP), whose expression attenuates EGFR activation. (aacrjournals.org)
- The epidermal growth factor receptor (EGFR) tyrosine kinase (TK) plays an important role in the pathogenesis of NSCLC. (bmj.com)
- Alterations in receptor tyrosine kinases (TKs), such as the epidermal growth factor receptor (EGFR) and insulin-like growth factor receptor 1, include overexpression, amplification or mutations. (bmj.com)
- Novel therapeutic agents, in particular those that specifically target members of the human epidermal growth factor receptor (HER (ErbB1)) pathway, have shown encouraging therapeutic efficacy. (bmj.com)
- Receptor tyrosine-protein kinase erbB-2, also known as HER2/Neu (human epidermal growth factor receptor 2), is a member of the human epidermal growth factor receptor family. (meridianbioscience.com)
- MA5-14485 targets Epidermal Growth Factor Receptor in IHC (P) applications and shows reactivity with Human samples. (thermofisher.com)
- EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. (thermofisher.com)
- EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. (thermofisher.com)
- This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. (arigobio.com)
- Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) have been emerged as the standard selection in non-small cell lung cancer (NSCLC) patients with EGFR sensitive mutations. (biomedcentral.com)
- Owing to the well-established beneficial outcomes of clinical trials, epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs), have been approved as the standard regimens in patients with non-small cell lung cancer (NSCLC) harboring EGFR sensitive mutations [ 1 ]. (biomedcentral.com)
HER214
- Detects human ErbB2/Her2 in direct ELISAs. (rndsystems.com)
- Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. (rndsystems.com)
- MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB9589N, filled histogram) or irrelevant antibody (open histogram). (rndsystems.com)
- Recombinant human ErB2 / Her2 (1129-ER) blocks anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer proliferation. (novusbio.com)
- 875. The ED 50 for this effect is 0.3-1.2 μg/mL in the presence of 0.6 µg/mL Anti-Human ErbB2/Her2 Monoclonal Antibody (Trastuzumab, Catalog # MAB9589 ). (novusbio.com)
- The HER2 receptor does not bind to any known ligands, however, its dimerization arm is innately positioned in an open conformation. (plos.org)
- Confocal immunofluorescent analysis of MDA-MB-453 cells (left) and MDA-MB-231 cells (right), using HER2/ErbB2 (29D8) Rabbit mAb (green). (cellsignal.com)
- Flow cytometric analysis of MDA-MB-468 cells (blue) and SKBR3 cells (green) using HER2/ErbB2 (29D8) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). (cellsignal.com)
- Western blot analysis of cell extracts from various cell lines, using HER2/ErbB2 (29D8) Rabbit mAb. (cellsignal.com)
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HER2/ErbB2 (29D8) Rabbit mAb. (cellsignal.com)
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HER2/ErbB2 (29D8) RmAb in the presence of control peptide (left) or HER2/ErbB2 Blocking Peptide #1059 (right). (cellsignal.com)
- Immunohistochemical analysis of paraffin-embedded SKBr3 (high HER2) (left), MDA-MB-453 (moderate HER2) (middle) and MDA-MB-468 (low HER2) (right), using HER2/ErbB2 (29D8) Rabbit mAb. (cellsignal.com)
- Synthetic peptide around the C-terminus of Human HER2 / ErbB2. (arigobio.com)
- Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG66357 anti-ERBB2 / HER2 antibody [SQab1890] at 1:500 dilution. (arigobio.com)
Signaling pathway1
- Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. (arigobio.com)
EGFR8
- It inhibits both ERBB2 and EGFR activity. (antibodyguide.com)
- In the current study, we examined the effects of recombinant ERRP on the growth and ligand-induced activation of multiple members of erbB family in three pancreatic cancer cell lines that express varying levels of EGFR and other member(s) of its family, specifically HER-2. (aacrjournals.org)
- ERRP also inhibited ligand-induced activation of EGFR, HER-2, and HER-3 (ErbB3). (aacrjournals.org)
- In contrast, Erbitux and Herceptin only partially or modestly inhibited activation of EGFR, HER-2, and HER-3. (aacrjournals.org)
- The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). (activemotif.com)
- EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. (thermofisher.com)
- Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. (thermofisher.com)
- Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. (thermofisher.com)
Overexpression7
- 32 % of patients exhibited overexpression of HER-2/neu. (thieme-connect.de)
- Prospective studies correlating HER-2/neu overexpression with prognosis might provide additional data. (thieme-connect.de)
- c-erbB-2 protein overexpression in breast cancer is a risk factor in patients with involved and uninvolved lymph nodes. (thieme-connect.de)
- This family of RTKs has been highly implicated in the formation and progression of various cancers via aberrant overexpression, kinase activation, and mutation [1] , [2] . (plos.org)
- Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. (activemotif.com)
- Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. (activemotif.com)
- ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. (activemotif.com)
Gene4
- This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. (creativebiolabs.net)
- ERBB2 gene amplification detected by fluorescent differential polymerase chain reaction in paraffin-embedded breast carcinoma tissues. (thieme-connect.de)
- 5 Beckmann M W, Niederacher D, Schnurch H G, Bender H G. The erbB gene family: significance for tumor development, prognosis and new therapeutic modalities. (thieme-connect.de)
- 14 King C R, Kraus M H, Aaronson S A. Amplification of a novel v-erbB-related gene in a human mammary carcinoma. (thieme-connect.de)
Antibody8
- Trastuzumab (Herceptin) is an antibody that targets ERBB2 and has been approved for the treatment of breast cancer. (antibodyguide.com)
- Immunogen: This Mouse Erbb2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the Central region of mouse Erbb2. (hatinhibitor.com)
- Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. (cellsignal.com)
- The Y877 kit contains phospho-ErbB-2 antibody that recognizes ErbB-2 only when phosphorylated at Tyr877, while the Y1248 kit provides a phospho-antibody that recognizes ErbB-2 only when phosphorylated at Tyr1248. (activemotif.com)
- Each kit contains a total-ErbB-2 antibody that recognizes ErbB-2 regardless of its phosphorylation state. (activemotif.com)
- The FACE ErbB-2 (Y877) Kit contains a phospho-ErbB-2 antibody that was raised in rabbit against a synthetic phospho-peptide corresponding to residues surrounding phosphorylated Tyr877 of human ErbB-2 and recognizes ErbB-2 only when phosphorylated at Tyr877. (activemotif.com)
- The total-ErbB-2 antibody supplied in the FACE ErbB-2 Kits recognizes ErbB-2 protein regardless of its phosphorylation site. (activemotif.com)
- For continuous use, store undiluted antibody at 2-8°C for up to a week. (arigobio.com)
Protein4
- The degradative pathway of ErbB receptor tyrosine kinases stimulated by tyrosine kinase inhibitors appears to be chaperone mediated, and thus is similar to the pathways activated by the heat shock protein 90 (Hsp90) antagonist geldanamycin and by stress-induced mechanisms. (lclabs.com)
- A cell surface protein-tyrosine kinase receptor that is overexpressed in a variety of ADENOCARCINOMAS. (bvsalud.org)
- However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. (arigobio.com)
- Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. (arigobio.com)
Prognosis2
- The HER-2/neu onkogene may play a role in the prognosis and management of patients with breast cancer. (thieme-connect.de)
- 1, 2 Most studies of MUC1 expression in breast carcinomas have shown that increased membrane MUC1 mucin expression on the apical cell surface is associated with a better prognosis (reviewed in Rahn and colleagues 2 ), whereas circumferential staining in tumour cell cytoplasm is associated with a worse prognosis. (bmj.com)
Ligand-bound1
- Activation of the erbB-2 receptor occurs through heterodimer formation with a ligand-bound erbB receptor family member. (bvsalud.org)
Antibodies2
- Sensitivity of HER-2/neu antibodies in archival tissue samples of invasive breast carcinomas. (thieme-connect.de)
- Each FACE ErbB-2 Kit provides 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total ErbB-2. (activemotif.com)
Stratified by hormone receptor1
- We did the randomisation via an interactive response system under a permuted block randomisation scheme (block size of four), stratified by hormone receptor status, stage at diagnosis, and geographical location. (nih.gov)
Phosphorylation4
- This process leads to the activation of each receptors' tyrosine kinase and the subsequent phosphorylation of tyrosine residues located on their C-terminal tails. (plos.org)
- It not only inhibits tyrosine phosphorylation but also enhances ubiquitinylation and accelerates endocytosis and subsequent intracellular destruction of ErbB-2 molecules. (lclabs.com)
- Canertinib inhibited erbB receptor phosphorylation and induced growth inhibition and apoptosis at concentrations of 1 µM or more. (lclabs.com)
- Essential erbB family phosphorylation in osteosarcoma as a target for CI-1033 inhibition. (lclabs.com)
Kinase2
- Brand TM, Iida M, Luthar N, Wleklinski MJ, Starr MM, Wheeler DL (2013) Mapping C-Terminal Transactivation Domains of the Nuclear HER Family Receptor Tyrosine Kinase HER3. (plos.org)
- Canertinib (CI-1033, PD 183805) is a pan-erbB tyrosine kinase inhibitor. (lclabs.com)
Targets3
- Lapatinib (Tykerb) is another drug that targets ERBB2. (antibodyguide.com)
- Discovering a specific agent, which targets multiple members of the erbB family, would be important in pancreatic cancer therapy. (aacrjournals.org)
- Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments. (activemotif.com)
Extracellular domain1
- It binds to the extracellular domain of ERBB2 and inhibits its activity. (antibodyguide.com)
Residues2
- Phosphorylated tyrosine residues recruit various intracellular adaptor and effector molecules that result in the propagation of growth promoting signal transduction cascades [1] , [2] . (plos.org)
- Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). (arigobio.com)
Tumor2
- Nuclear localized HER family receptor tyrosine kinases (RTKs) have been observed in primary tumor specimens and cancer cell lines for nearly two decades. (plos.org)
- Membrane-bound HER receptors activate numerous tumor promoting signaling cascades via this mechanism, including the PI3K/AKT, Ras/Raf/Mek/Erk, PLCγ/PKC, and signal transducer and activator of transcription (STAT) pathways [1] , [2] . (plos.org)
Cognate ligands1
Inhibitor1
- Radiosensitization by pan ErbB inhibitor CI-1033 in vitro and in vivo. (lclabs.com)
Activation1
- Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. (creativebiolabs.net)
Cancers1
- The erbB family of receptor tyrosine kinases plays critical roles in human cancers, including pancreatic cancer. (aacrjournals.org)
Node-positive breast cancer1
- HER-2/neu amplification predicts poor survival in node-positive breast cancer. (thieme-connect.de)
Immunohistochemical1
- 2 Barnes D M, Lammie G A, Millis R R, Gullick W L, Allen D S, Altman D G. An immunohistochemical evaluation of c-erbB-2 expression in human breast carcinoma. (thieme-connect.de)
Confer1
- However, the regions of each receptor that confer transcriptional potential remain poorly defined. (plos.org)
Human1
- Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. (novusbio.com)
Family1
- While the classical membrane-bound functions of HER family RTKs have been extensively studied, accumulating data suggest that these receptors can be found in the cell's nucleus where they can function as co-transcriptional activators [4] , [5] . (plos.org)
Amplification1
- 7 Borg A, Baldetorp B, Ferno M, Killander D, Olsson H, Sigurdsson H. ERBB2 amplification in breast cancer with a high rate of proliferation. (thieme-connect.de)
Therapeutic1
- ERBB2 has been identified as a therapeutic target for breast cancer therapy. (antibodyguide.com)
Breast cancer2
- ERBB2 is overexpressed in many types of cancer, including breast cancer, ovarian cancer, and gastric cancer. (antibodyguide.com)
- DESIGN, SETTING, AND PARTICIPANTS: The I-SPY2 is a multicenter, platform adaptive, randomized clinical trial in the US that compares, by subtype, investigational agents in combination with chemotherapy vs chemotherapy alone in adult women with stage 2/3 breast cancer at high risk of early recurrence. (providence.org)
Prognostic1
- This finding could indicate that HER-2/neu expression is a new independent prognostic factor. (thieme-connect.de)
Essential1
- Background: Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. (hatinhibitor.com)
Amino acids1
- These analyses identified a bipartite region of 34 (B 1 ) and 27 (B 2 ) amino acids in length that conferred the majority of HER3's transactivation potential. (plos.org)
Assay1
- The FACE ErbB-2 (Y1248) and ErbB-2 (Y877) Kits were used to assay the levels of total and phosphorylated ErbB-2 contained within untreated or EGF treated A-431 cells. (activemotif.com)
Lung2
- 2 The pathogenesis of lung cancer involves the accumulation of several molecular abnormalities over time. (bmj.com)
- One of the lesions in right lung obtained by percutaneous lung biopsy (PNLB) was proved to be adenocarcinoma (Fig. 2 a). (biomedcentral.com)