Loading...
  • antibody
  • The network is often dysregulated in cancer and lends credence to the mantra that molecular understanding yields clinical benefit: over 25,000 women with breast cancer have now been treated with trastuzumab (Herceptin), a recombinant antibody designed to block the receptor ErbB2. (nih.gov)
  • Therefore, relatively few patients with lung cancer are likely to benefit from treatment with trastuzumab, the humanized monoclonal antibody that is effective in the 20% of patients with breast cancer and HER-2 gene amplification and/or 3+ staining by immunohistochemistry. (aacrjournals.org)
  • This observation prompted the development of a humanized monoclonal antibody directed against the extracellular domain of the HER-2 receptor, trastuzumab ( 10 ). (aacrjournals.org)
  • The following antibody was used in this experiment: ErbB2 Monoclonal Antibody (2G11), Biotin from Thermo Fisher Scientific, catalog # AHO0919, RRID AB_2536319. (thermofisher.com)
  • tumor
  • We investigated the therapeutic activity of an agonist mAb to mouse tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor-2 (DR5) against ErbB2-driven breast cancer. (pnas.org)
  • The high levels of the erbB-2 product associated with malignant transformation of NIH/3T3 cells were observed in human mammary tumor cells that overexpressed this gene 3 . (apexbt.com)
  • Pertuzumab can have antitumor activity in patients with HER-2 present on the tumor without gene amplification or 3+ staining by immunohistochemistry. (aacrjournals.org)
  • Down-regulation of ErbB2 and inhibition of HAEC growth by cocoa procyanidins may have several downstream implications, including reduced vascular endothelial growth factor (VEGF) activity and angiogenic activity associated with tumor pathology. (biomedsearch.com)
  • Tid1, the human homologue of a Drosophila tumor suppressor, reduces the malignant activity of ErbB-2 in carcinoma cells. (nih.gov)
  • We discovered that this domain of ErbB-2 interacts with Tid1 protein, the human counterpart of the Drosophila tumor suppressor Tid56, whose null mutation causes lethal tumorigenesis during the larval stage. (nih.gov)
  • Importantly, ErbB-2-dependent tumor progression in animals is inhibited by increased expression of Tid1 in tumor cells. (nih.gov)
  • Collectively, these results suggest that Tid1 modulates the uncontrolled proliferation of ErbB-2-overexpressing carcinoma cells by reducing ErbB-2 expression and as a result suppresses the ErbB-2-dependent cancerous signaling and tumor progression. (nih.gov)
  • transgenic mice
  • 0,007), and in transgenic mice carrying the rat HER-2/neu oncogene. (biomedcentral.com)
  • Studies with transgenic mice have established that elevated expression of erbB2 in mammary epithelium can directly induce mammary carcinomas. (nih.gov)
  • suppresses
  • We found that increased expression of Tid1 in human mammary carcinomas overexpressing ErbB-2 suppresses the expression level of ErbB-2 and attenuates the resultant ErbB-2-dependent oncogenic extracellular signal-regulated kinase 1/2 and big mitogen-activated protein kinase 1 signaling pathways leading to programmed cell death (PCD). (nih.gov)
  • Human
  • Pathological expression of human ErbB-2 protein, also known as HER-2, is common in many types of cancer. (semanticscholar.org)
  • From a human genomic library, we obtained six v-erbB-related DNA clones. (semanticscholar.org)
  • Pentameric procyanidins isolated from Theobroma cacao seeds selectively downregulate ErbB2 in human aortic endothelial cells. (biomedsearch.com)
  • Consistent with this primary observation, the cocoa flavonoid fraction also suppressed human aortic endothelial cell (HAEC) growth and decreased expression of two tyrosine kinases responsive to ErbB2 modulation, namely VEGFR-2/KDR and MapK 11/p38beta2. (biomedsearch.com)
  • Natural and recombinant human ErbB2. (rndsystems.com)
  • It contains NS0-expressed recombinant human ErbB2 and has been shown to accurately quantitate the recombinant factor. (rndsystems.com)
  • Results obtained using natural human ErbB2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. (rndsystems.com)
  • These results indicate that this kit can be used to determine relative mass values for naturally occurring human ErbB2. (rndsystems.com)
  • The recovery of human ErbB2 spiked to levels throughout the range of the assay in various matrices was evaluated. (rndsystems.com)
  • To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. (rndsystems.com)
  • Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization. (expasy.org)
  • Recombinant human p185/HER-2. (thermofisher.com)
  • In the current study, we investigated the prognostic value of Notch receptors in human BC patients. (springer.com)
  • morphogenesis
  • Despite these perturbations in virgin mammary gland morphogenesis, targeted disruption of erbB2 had little impact on the ability of these animals to lactate. (nih.gov)
  • Taken together, these observations indicate that erbB2 plays a critical role in the initial stages of mammary gland morphogenesis. (nih.gov)
  • mammary
  • Targeted disruption of ErbB2/Neu in the mammary epithelium results in impaired ductal outgrowth. (nih.gov)
  • Here, we demonstrate that the mammary-specific ablation of erbB2 through Cre-mediated recombination leads to a striking ductal elongation defect. (nih.gov)
  • molecular
  • BACKGROUND: Biological molecular markers such as proto-oncogene erbB-2 (HER-2/neu, c-erbB-2), the CXC chemokine receptor 4 (CXCR4), estrogen receptor (ER), Proliferating Cell Nuclear Antigen (PCNA), DNA topoisomerase II (topo II), P-glycoprotein (P-gp) and glutathione S-transferase (GST) were observed for changes after administration of neochemotherapy and whether these protein expression changes were correlated with response to chemotherapy. (biomedsearch.com)
  • We report that the pentameric procyanidin (molecular weight of 1442 daltons) fraction isolated from cocoa was a potent inhibitor of tyrosine kinase ErbB2 expression, a receptor important in angiogenesis regulation. (biomedsearch.com)
  • potent
  • A further five- to tenfold increase in its expression under influence of the long terminal repeat of Moloney murine leukemia virus was associated with activation of erbB-2 as a potent oncogene. (apexbt.com)
  • erbB-2 is a Potent Oncogene When Overexpressed in NIH/3T3 Cells. (apexbt.com)
  • regulation
  • ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. (rndsystems.com)