Epitopes in peptide sequences. Medical search. Frequent questions

Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
The proteins that the phages are displaying can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those of other molecules. (wikipedia.org)
Phage eluted in the final step can be used to infect a suitable bacterial host, from which the phagemids can be collected and the relevant DNA sequence excised and sequenced to identify the relevant, interacting proteins or protein fragments. (wikipedia.org)
We identified 78 amino acid sequences from self proteins presented in the lungs of sarcoidosis patients, some of which were well-known autoantigens such as vimentin and ATP synthase. (jci.org)
We designed new peptides of GRA6, GRA7, and SAG1 proteins, with more SNPs among the three clonal strains than those previously designed. (frontiersin.org)
developed a typing method based on antibody binding to polymorphic peptides, designed from proteins related to virulence. (frontiersin.org)
Sets of overlapping peptides from potential target vaccine proteins are screened with immune sera or cells to find vaccine or diagnostic test candidates. (mimotopes.com)
They identified highly-conserved antigenic peptide sequences from a range of latent and lytic EBV proteins and included them serially by separating each sequence using proteolytic cleavage sites. (news-medical.net)
This study was undertaken to identify antigenic epitopes on two of the surface glycoproteins of BRSV, the fusion (F) and attachment (G) proteins, the major surface viral antigens, for inclusion into a novel subunit peptide vaccine. (alliedacademies.org)
This review reports a comprehensive update of the current scientific knowledge about proteins and peptides of MAP with already documented humoral response. (degruyter.com)
Transgenic proteins were used by GMF are evaluated on the basis of potential allergenic properties, identification of short identical amino acid sequences were detected from transgenic proteins In this study, bioinformatics approach had been used to detect the potential allergens motifs in amino acid sequences. (ispub.com)
Different types of transgenic proteins food allergen proteins sequences were scanned for IgE epitopes. (ispub.com)
We have scanned total 52 proteins for detection of allergenic IgE epitopes. (ispub.com)
From a compilation of known antigenic peptides, two algorithms have been proposed to predict antigenic sites in proteins. (uni-muenchen.de)
Given the small size of this peptide, it is unlikely that modifying proteins with it will affect protein structure or function. (qedbio.com)
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. (gentaur.com)

The antibodies from people infected by hookworm can recognize the dominant epitope of the antigen called Necator americanus APR-1 protein, but the antibodies can't induce protection against hookworm. (wikipedia.org)
However, other non-dominant epitopes on APR-1 protein show the ability to induce the production of neutralizing antibodies against hookworm. (wikipedia.org)
Probing the wells with antibody and washing away unbound antibodies quickly reveals those peptides to which the antibody has bound. (mimotopes.com)
In a typical example, a library of monoclonal antibodies to a small protein, thought to recognize linear epitopes, was screened using a PepSet of biotinylated peptides (Figure 2). (mimotopes.com)
Using this approach, the linear epitopes of several monoclonal antibodies were quickly and clearly identified. (mimotopes.com)
The monoclonal antibodies fell into groups recognizing one of three linear sequences of the protein. (mimotopes.com)
For indirect mapping, peptide-specific rabbit antibodies were raised to block IgE against surface-exposed epitopes on folded Art v 1. (nih.gov)
Differing epitope selection of experimentally-induced and natural antibodies to a disease-specific a. (edu.au)
Differing epitope selection of experimentally-induced and natural antibodies to a disease-specific autoantigen, the E2 subunit of pyruvate dehydrogenase complex (PDC-E2). (edu.au)
Moreover, the experimentally-induced antibodies in striking contrast to the natural autoantibodies showed preferential reactivity with PDC-E2 rather than with intact PDC, failed to inhibit in vitro the catalytic function of PDC, and, on peptide scanning, reacted with discrete epitopes, but at sites other than the lipoyl-binding region of PDC-E2. (edu.au)
In atopic individuals, sensitizing T-cell epitopes can trigger a cascade of events that leads to synthesis of allergen-specific immunoglobulin E (IgE) antibodies as well as other immunological reactions. (ispub.com)
abstract = 'In this study, polyclonal and monoclonal antibodies to native and denatured chicken ovalbumin (OVA) were produced to compare their dependency on continuous and three-dimensional epitopes. (ku.dk)
These antibodies were characterized with respect to reactivity to native and denatured OVA by enzyme-linked immunosorbent assay (ELISA) employing surface-bound OVA and streptavidin-capture ELISA to determine whether effects of different coating influence antibody specificity and with respect to epitope specificity by peptide ELISA, using overlapping peptides, covering the complete OVA sequence. (ku.dk)
Polyclonal antibodies to native OVA reacted strongly with native and denatured OVA in both assays, but did not react with the overlapping peptides. (ku.dk)
Polyclonal antibodies to denatured OVA reacted strongly with both OVA forms and with several of the overlapping peptides. (ku.dk)
Monoclonal antibodies to native OVA reacted preferentially with three-dimensional epitopes on native OVA and not with denatured OVA. (ku.dk)
Two of these monoclonal antibodies, HYB 94-06 and 94-07, showed reactivity to overlapping peptides and their epitopes were identified as flexible structures constituting amino acids 130-135 and 136-141, respectively. (ku.dk)
Collectively, immunization with native OVA preferentially generates highly specific antibodies reacting with three-dimensional epitopes, whereas immunization with denatured OVA generates antibodies occasionally reacting with continuous epitopes. (ku.dk)
Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. (gentaur.com)
These 2 antibodies target epitopes located on the peptide core of the extracellular domain of MUC1. (medscape.com)
Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)

The whole peptide vaccine is to mimic the epitope of an antigen, so epitope design is the most important stage of vaccine development and requires an accurate understanding of the amino acid sequence of the immunogenic protein interested. (wikipedia.org)
Spike glycoprotein (PDB Id: 6VSB) of the coronavirus was selected and analysed using immune epitope database server for prediction of potential immunogenic B and T-cell epitopes. (ijpsonline.com)
Background Neoantigens, new immunogenic sequences arising from tumor mutations, have been associated with response to immunotherapy and are considered potential targets for vaccination. (unav.edu)
Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide. (enzolifesciences.com)
The following synthetic S-arylcysteines were incorporated into 10-mer head sequences of K1 [GGGRFSS(S-aryl-C)GG] and K10 [GGGG(S-aryl-C)GGGGG] to form the predicted immunogenic epitopes for antibody production for ELISA: S-phenylcysteine-K1 (SPK1), S-phenylcysteine-K10 (SPK10), S-(1-naphthyl)cysteine-K1 (1NK1), S-(1-naphthyl)cysteine-K10 (1NK10), S-(2-naphthyl)cysteine-K1 (2NK1), and S-(2-naphthyl)cysteine-K10 (2NK10). (cdc.gov)

E75 consisting of 9 amino acids is the immunodominant epitope of the HER2 protein. (wikipedia.org)
Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
Here, we have demonstrated that endogenous processing and presentation of a human immunodominant HIV-1 epitope is more efficient than that of a subdominant epitope. (jci.org)
Furthermore, we have shown that the regions flanking the immunodominant epitope constitute a portable motif that increases the production and antigenicity of otherwise subdominant epitopes. (jci.org)
We used a novel in vitro degradation assay involving cytosolic extracts as well as endogenous intracellular processing assays to examine 2 well-characterized HIV-1 Gag overlapping epitopes presented by the same HLA class I allele, one of which is consistently immunodominant and the other subdominant in infected persons. (jci.org)
The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
Notably, cytosolic digestion experiments revealed flanking residues proximal to the immunodominant epitope that increased the production and antigenicity of otherwise subdominant epitopes. (jci.org)
The immunodominant epitope for these autoantibodies is associated with the lipoyl-binding domain of the E2 subunit of the enzymes. (edu.au)
The immunogens included the intact pyruvate dehydrogenase complex (PDC) from bovine heart, human recombinant PDC-E2, and short synthetic peptides representing the immunodominant lipoic acid binding sequences of the 2-OADC enzymes. (edu.au)
Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. (elifesciences.org)

In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. (wikipedia.org)

A single peptide vaccine can be designed to have multiple epitopes to generate immune responses for several diseases. (wikipedia.org)
Gp100 peptide vaccine is studied to treat melanoma. (wikipedia.org)
Rindopepimut is the epidermal growth factor receptor (EGFR)-derived peptide vaccine to treat glioblastoma multiforme (GBM). (wikipedia.org)
EpiVacCorona, a peptide-based vaccine against COVID-19. (wikipedia.org)
IC41 is a peptide vaccine candidate against the Hepatitis C virus. (wikipedia.org)
Multimeric-001 is the most efficient peptide vaccine candidate against influenza. (wikipedia.org)
Such portable epitope processing determinants provide what we believe is a novel approach to optimizing CTL responses elicited by vaccine vectors. (jci.org)
In the current study immunoinformatics procedures were employed to introduce a novel multi-epitope subunit vaccine. (ijpsonline.com)
This multi-epitope vaccine can activate equally class I and II human leukocyte antigen and antibody mediated immune responses. (ijpsonline.com)
The two dimensional and three dimensional structure of multi-epitope vaccine were predicted and authenticated using PROCheck and Raptor-X servers. (ijpsonline.com)
Docking results of the multi-epitope vaccine peptide with human leukocyte antigen class I and II alleles predicted efficient binding and the resulted docked models were stable during simulation. (ijpsonline.com)
Scientists from Australia and the USA have developed a lymph node-targeted multi-epitope subunit vaccine that induces strong humoral and cellular immune responses against Epstein-Barr virus (EBV) in mice. (news-medical.net)
In this study, scientists have developed a lymph node-targeting multi-epitope subunit vaccine against EVB and tested its efficacy in Human Leukocyte Antigen (HLA)-expressing mice. (news-medical.net)
Scientists analyzed known human CD8+ T cell epitopes from EBV antigens to design a polyepitope vaccine immunogen that incorporates 20 CD8+ T cell epitopes into an engineered protein immunogen. (news-medical.net)
The testing of the polyepitope vaccine immunogen using peripheral mononuclear cells isolated from EBV seropositive donors revealed that the immunogen can be effectively processed by antigen-presenting cells to generate epitopes that can be presented on HLA class I molecules and subsequently recognized by human CD8+ T cells to promote an immune response. (news-medical.net)
Identification of the new major conformational IgE binding site on Art v 1, which can be blocked with IgG raised against non-IgE reactive Art v 1 peptides, is an important basis for the development of a hypoallergenic peptide vaccine for mugwort allergy. (nih.gov)
Thus this peptide appears a good candidate for developing an immune target vaccine strategy possibly synergizing with TKIs for remission maintenance. (hindawi.com)
Thus, from an immunologic point of view, peptides derived from p190-breakpoint area are leukemia-specific antigens that may be employed as therapeutic vaccine with the purpose to induce a T cell response toward p190+ leukemia cells. (hindawi.com)
In the present work we searched for p190-derived breakpoint peptides suitable for a peptide vaccine approach in vivo. (hindawi.com)
Previously, we have developed a p210-breakpoint derived penta-peptide vaccine for controlling minimal residual disease in Chronic Myeloid Leukemia (CML) patients treated with imatinib [ 7 ]. (hindawi.com)
In this setting, we found that the best antileukemia immune response was mediated by CD4+ T cells specific for an HLA class II "size" p210 breakpoint-derived peptide included in the vaccine. (hindawi.com)
Both proteomics, peptidomics and bioinformatics represent powerful complementary tools to discover specific immunoreactive peptides useful for diagnosis or vaccine. (degruyter.com)
Clustered Iridoviridae subfamilies possess host-specific characteristics, which can be considered as exclusive features for in-silico prediction of effective epitopes for vaccine development. (biomedcentral.com)
Our proposed voting mechanism based system provides a novel approach for in silico LE prediction prior to vaccine development, and it is especially powerful for analyzing antigen sequences with exclusive features between two clustered groups. (biomedcentral.com)
In general, immunobiologists have developed an integrated method for vaccine development based on analyzing protein sequences and structures of target viruses [ 10 ]. (biomedcentral.com)
Although a variety of techniques are available to assist in the selection of mutation-defined epitopes to be included within the tumor vaccine, the ability of the peptide to bind to patient MHC is a key gateway to peptide presentation. (broadinstitute.org)
Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine. (microbiologyresearch.org)

Peptide vaccines can not only induce protection against infectious pathogens and non-infectious diseases but also be utilized as therapeutic cancer vaccines, where peptides from tumor-associated antigens are used to induce an effective anti-tumor T-cell response. (wikipedia.org)
peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
Signal/cutoff (S/C) dis- tribution of Center for Disease Control (Taiwan) SARS (severe peptide antigens as the solid-phase immunosorbent. (cdc.gov)
Peptides derived from p190-breakpoint area are leukemia-specific antigens that may mediate an antitumor response toward p190+ leukemia cells. (hindawi.com)

Peptide-based synthetic vaccines (epitope vaccines) are subunit vaccines made from peptides. (wikipedia.org)

Immunogen Synthetic peptide corresponding to aa 314-319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. (qedbio.com)

The peptides can be specially designed for specificity. (wikipedia.org)
SARS Antibody Test for Serosurveillance a standardized and rapid peptide-based SARS ELISA is characterized for sensitivity and specificity. (cdc.gov)
The candidate peptides used for this purpose must have several properties principally represented by their specificity and their location in the bacterial cell. (degruyter.com)
a webtool for the prediction of T-cell receptor sequence epitope specificity. (biostars.org)

Harinder Singh, Hifzur Rahman Ansari and Gajendra pal Singh Raghava: LBtope: Improved method for linear B-cell epitope prediction using antigen's primary sequence. (imtech.res.in)
A novel, linear B-cell epitope has been identified at the N terminus of the rabies virus (RABV) glycoprotein. (microbiologyresearch.org)

To induce an immune response, a sequence from yeast GCN4 protein is used to improve the conformation of the peptide vaccines by forming alpha-helix. (wikipedia.org)
Use adjuvants associated with the epitope to induce the immune response. (wikipedia.org)
The inclusion of both latent and lytic antigenic epitopes is intended to induce robust T-cell response targeting different phases of the viral life cycle. (news-medical.net)
We identified one peptide named p190-13 able to induce in vitro peptide-specific CD4+ T cell proliferation in Ph+ ALL patients in complete remission during TKIs. (hindawi.com)
We conducted a pilot randomized phase I trial in PC patients to determine the peptide dose required to induce an optimal CD8 + T cell response. (bmj.com)
In a multimeric form, the peptide could induce an epitope-specific response following immunization in rabbits and mice. (microbiologyresearch.org)

Bharat Panwar, Sudheer Gupta and Gajendra Pal Singh Raghava : VitaPred- Software for the sequence based prediction of vitamin interacting residues using evolutionary information. (imtech.res.in)
Jagat Singh Chhauhan, Adil H. Bhat, Alka Rao and Gajendra pal Singh Raghava : GlycoPP: A Webserver for Prediction of N- O-Glycosites in Prokaryotic Protein Sequences. (imtech.res.in)
Nishant Thakur, Abid Qureshi and Manoj Kumar : AVPpred: A software for the prediction of highly effective antiviral peptides. (imtech.res.in)
NPTOPE for the prediction of non-peptide epitopes using QSAR based Random Forest model. (imtech.res.in)
Host-specific characteristics are important features and constraints for effective epitope prediction. (biomedcentral.com)
sequence-based prediction of TCR binding to peptide-MHC complexes using convolutional neural networks. (biostars.org)
epitope immunogenicity prediction through in silico TCR-peptide contact potential profiling. (biostars.org)
This site provides a collection of tools for the prediction and analysis of immune epitopes. (biostars.org)

We implemented a strategy to determine the serotype based on scatter plots and a mathematical formula, using ratios among reactivity indexes to peptides. (frontiersin.org)
The chronicity of the infection negatively affected the reactivity index against the peptides. (frontiersin.org)
Four overlapping peptides incorporating surface-exposed amino acids representing the full-length Art v 1 sequence were synthesized and used to search for IgE reactivity to sequential epitopes. (nih.gov)
Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. (nih.gov)
The peptide sequence is conserved in mammals, birds, and amphibians, giving the antibody broad species reactivity. (cytoskeleton.com)
One person had strong humoral and weak cellular immune reactivity to SIVcol peptides. (cdc.gov)

A peptide-based enzyme-linked immunosorbent assay exists and signals the need for continued surveillance with (ELISA) can be used for retrospective serosurveillance of laboratory testing. (cdc.gov)
The new tion (RT-PCR) for detecting viral RNA is the most sensi- peptide ELISA consistently detected seroconversion by tive method for early identification of SARS. (cdc.gov)
The results from a retrospective serologic survey by the peptide ELISA of healthcare workers from facilities affected by nosocomial outbreaks are presented as a working example. (cdc.gov)
Antibody epitope mapping by ELISA is simple to perform using biotinylated peptides because the peptides can be captured onto Streptavidin™ or NeutrAvidin™ coated plates through the strong noncovalent avidin-biotin binding. (mimotopes.com)
The set consisted of short overlapping peptides derived from the protein being investigated and the peptides were captured onto plate wells coated with streptavidin before completing an ELISA with each monoclonal antibody (Figure 1). (mimotopes.com)
The 1-3mg scale PepSet is enough peptide for several thousand ELISA tests. (mimotopes.com)

Synthetic peptide corresponding to Human C3d (N terminal). (abcam.com)
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Vimentin. (lsbio.com)
Synthetic peptide corresponding to an N-terminal sequence of human CD163. (enzolifesciences.com)
A 12 aa synthetic peptide of this epitope was recognized by both mAbs and sera from a subset of rabies-vaccinated dogs. (microbiologyresearch.org)

Furthermore, specific point mutations in these portable flanking sequences modulated the production and antigenicity of epitopes. (jci.org)
Analogous to the 33-mer, the synthetic 26-mer peptide displayed markedly enhanced T cell antigenicity compared to monovalent control peptides. (nih.gov)
Prolyl endopeptidase treatment was shown to abolish the antigenicity of both the 33-mer and the 26-mer peptides, and was also predicted to have comparable effects on other proline-rich putatively immunotoxic peptides identified from other polypeptides within the gluten proteome. (nih.gov)
One algorithm uses linear motifs in the sequence14, whereas the other considers peptide conformation and predicts antigenicity for amphipathic -helices15,16. (uni-muenchen.de)

With more advanced solid-phase peptide synthesis (SPPS) using automation and microwave techniques, the production of peptides becomes more efficient. (wikipedia.org)

To generate a greater in vitro CTL response, the peptide, gp100:209-217(210M), is modified and binds to HLA-A2*0201. (wikipedia.org)

This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. (abcam.com)

The dominant epitope was mapped using recombinant vaccinia viruses that expressed fragments of the NP and then synthetic peptides based on the NP amino acid sequence. (ox.ac.uk)
Secondary structure of recombinant (r) Art v 1 and peptides was determined by circular dichroism spectroscopy. (nih.gov)
Anti-SUMO-2/3 mouse monoclonal antibody was raised against full-length recombinant SUMO-2 protein (Uniprot: P61956) combined with a proprietary mix of peptides that include CQIRFRFDGQPINE. (cytoskeleton.com)

Linear antibody epitopes are readily mapped with PepSets. (mimotopes.com)

Some IgE-inducing epitopes cause hypersensitivity reactions after vaccination in humans due to the overlap with IgG epitopes in the Na-ASP-2 protein which is an antigen from hookworm. (wikipedia.org)
In conclusion, serotyping using the improved GRA6 peptide triad is useful to serotype T. gondii in humans and could be implemented for clinical management and epidemiological studies, to provide information on the parasite type in specific areas. (frontiersin.org)
Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)

Abid Qureshi, Nishant Thakur and Manoj Kumar : HIPdb: A database of experimentally validated HIV inhibiting peptides. (imtech.res.in)
It serves as a companion site to the Immune Epitope Database (IEDB) , a manually curated database of experimentally characterized immune epitopes. (biostars.org)

In silico immune evaluation using C-ImmSim server showed that the peptide could concurrently elicit cell-mediated and humoral immune responses. (ijpsonline.com)
Finally, in silico analysis of the gluten proteome led to the identification of at least 60 putative peptides that share the common characteristics of the 33-mer and the 26-mer peptides. (nih.gov)

This displayed the different peptides on the outer surfaces of the collection of viral clones, where the screening step of the process isolated the peptides with the highest binding affinity. (wikipedia.org)
This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
The techniques for antibody analysis included immunofluorescence, immunoblotting on mitochondrial extracts, ELISAs using entire PDC, PDC-E2, or synthetic peptides, epitope mapping by peptide scanning on overlapping octameric peptides representing the human PDC-E2 sequence, affinity purification on PDC-E2, and inhibition in vitro by sera of the catalytic function of PDC. (edu.au)
With advances in the accuracy of predictive algorithms for MHC class I binding, choosing epitopes on the basis of predicted affinity provides a rapid and unbiased approach to epitope prioritization. (broadinstitute.org)

The followings are the points to consider when designing the epitope: The non-dominant epitope could generate a stronger immune response than the dominant epitope. (wikipedia.org)

Also, some short peptides can be stabled or cyclized together to maintain the proper conformation. (wikipedia.org)

Second, proteolytic analysis of a representative gamma-gliadin led to the identification of another multivalent 26-mer peptide that was also resistant to further gastric, pancreatic and intestinal brush border degradation, and was a good substrate of human transglutaminase 2 (TG2). (nih.gov)
1] Ansari, H. R., Raghava, G. P., Identification of conformational B-cell Epitopes in an antigen from its primary sequence. (degruyter.com)
Cancer genome sequencing has enabled the rapid identification of the complete repertoire of coding sequence mutations within a patient's tumor and facilitated their use as personalized immunogens. (broadinstitute.org)

Probable anchor residues were identified at peptide residues 3 (lysine/arginine), 5 (lysine/arginine) and 9 (leucine/isoleucine). (ox.ac.uk)
A model was made of the HLA B8 molecule and negatively charged pockets predicted, which could accommodate the positively charged side chains of the peptide anchor residues. (ox.ac.uk)
the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
In such a trimolecular interaction the amino-acid sequence of the peptide must specify the contact with both receptors: agretope residues bind to the MHC receptor and epitope residues bind to the T-cell antigen receptor12,13. (uni-muenchen.de)

Further evaluation of 1 mg CEA peptide with stronger adjuvants, and/or combined with agents to overcome immune inhibitory pathways, may be warranted in PC pts. (bmj.com)

B-cell epitopes could only have 5 amino acids. (wikipedia.org)
GP2 consisting of 9 amino acids is the subdominant epitope. (wikipedia.org)

Rodda, S.J. (2002) Peptide Libraries for T-Cell Epitope Screening and Characterization. (mimotopes.com)

Blocking of allergic patients' IgE with peptide-specific rabbit antisera identified a hitherto unknown major conformational IgE binding site in the C-terminal Art v 1 domain. (nih.gov)
of which genuine peptide/antisera binding occurred with 4 peptides. (alliedacademies.org)

The autoantibodies bind to their reactive epitopes in the basement membranes and activate the complement cascade, resulting in tissue injury. (medscape.com)
Together, these results highlight the pivotal role of physiologically generated, proteolytically stable, TG2-reactive, multivalent peptides in the immune response to dietary gluten in Celiac Sprue patients. (nih.gov)

A sequence pattern for peptides presented to cytotoxic T lymphocytes by HLA B8 revealed by analysis of epitopes and eluted peptides. (ox.ac.uk)
T-cell epitopes (Helper and Cytotoxic) are easily located using PepSets provided peptides of the correct length are used2. (mimotopes.com)
This application is summarized in a separate article entitled Truncated Peptide Libraries for Cytotoxic T-Cell Epitope Mapping, available on the Mimotopes website. (mimotopes.com)
p210-breakpoint peptide-specific CD4+ T cells isolated from vaccinated patients were found to be either perforin+ or CD25+/Foxp3+: in both cases they exerted direct cytotoxic activity against a CML cell line [ 8 ]. (hindawi.com)

The spike glycoprotein sequence was retrieved from protein data bank (PDB) database. (ijpsonline.com)
Screening of a phage-display library demonstrated that two glycoprotein-specific mAbs recognized a conserved sequence, WxxxDI, which aligned between aa 14 and 19 of the mature glycoprotein. (microbiologyresearch.org)
Screening of truncated glycoprotein fragments with both mAbs confirmed the location of the epitope in the N-terminal region. (microbiologyresearch.org)
Cancer antigen 15-3, or CA 15-3, is an epitope of a large transmembrane glycoprotein named MUC1 that is derived from the MUC1 gene. (medscape.com)

Immune recognition surface construction of Mycobacterium tuberculosis epitope-specific antibody responses in tuberculosis patients identified by peptide microarrays. (cdc.gov)

In this issue of the JCI , two separate studies on MHC-bound peptides reopen the debate on the utility of peptides for the purposes of vaccination and treatment of autoimmune diseases. (jci.org)

This approach can be extended to characterize HLA-bound peptides in various autoimmune settings. (jci.org)
We sought to characterize IgE epitopes of Art v 1-sensitized patients and humanized mice for molecular immunotherapy of mugwort allergy. (nih.gov)

Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. (jci.org)

Some short peptide epitopes need elongating to maintain the native conformation. (wikipedia.org)

Support for this pattern came from sequencing peptides eluted from purified HLA B8 molecules, where lysines were predominant at positions 3 and 5. (ox.ac.uk)
Compared with the traditional vaccines such as the whole fixed pathogens or protein molecules, the peptide vaccines have several advantages and disadvantages. (wikipedia.org)

The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)

The MUC1 protein, also known as polymorphic epithelial mucin or epithelial membrane antigen, has a large extracellular region, a transmembrane sequence, and a cytosolic domain. (medscape.com)

Reverse transcription-polymerase chain reac- peptides from the spike, membrane, and nucleocapsid pro- tein sequences of SARS-associated coronavirus. (cdc.gov)
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This rate is patterns, gene sequencing, immuno- Centre for Disease Prevention and higher than that of severe acute respira- genicity and the animal-human and hu- Control (ECDC) and the National In- tory syndrome coronavirus (SARS), man-human interfaces. (who.int)

Broad Genomics Platform sequences a whole human genome every four minutes. (broadinstitute.org)
In November 2022, Broad's Genomics Platform sequenced its 500,000th whole human genome, a mere four years after sequencing its 100,000th. (broadinstitute.org)
Clinical and biological insights from viral genome sequencing. (cdc.gov)

Click molecule labels to explore molecular sequence information. (nih.gov)
Elution can be done combining low-pH elution buffer with sonification, which, in addition to loosening the peptide-target interaction, also serves to detach the target molecule from the immobilization surface. (wikipedia.org)
That a peptide must bind to an MHC molecule to interact with a T-cell antigen receptor is the molecular basis of the MHC restriction of antigen-recognition by T lymphocytes10,11. (uni-muenchen.de)

Peptides that are antigenic for T lymphocytes are ligands for two receptors, the class I or II glycoproteins that are encoded by genes in the major histocompatibility complex, and the idiotypic / chain T-cell antigen receptor1-9. (uni-muenchen.de)

Six HLA B8-positive influenza immune donors that we have tested respond to this peptide as part of their influenza-specific CTL response. (ox.ac.uk)

Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8+ T lymphocytes. (jci.org)

Binds to an epitope located within the peptide sequence MDPFDDDLKQ as determined by overlapping synthetic peptides. (thermofisher.com)

I believe that IEDB predicts MHC - epitope binding. (biostars.org)

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