The syndrome was subsequently shown to be caused by an antibody response against antigens present in the alveolar and glomerular basement membranes. (medscape.com)
The Col 4α3NC1 epitope is thought to be less accessible for anti-GBM binding in the lung, and partial denaturation of NC1 domains may be required for full exposure of this sequestered epitope to the antibody. (medscape.com)
Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
Interestingly, the recent data in RA indicate that the major HLA-DR associations are with anti-CCP antibody positive disease, suggesting that control of autoantibody responses may be a primary mechanism underlying these associations in RA as well. (musculoskeletalkey.com)
Alleles3
The association between aPL antibodies and particular human leukocyte antigen (HLA) alleles and HLA-linked epitopes has been reported in studies of patients with lupus erythematous (eg, HLA-DR7, HLA-DR4). (medscape.com)
The HLA-DR3 phenotypes seem to predispose to the formation of aCL antibodies and antinuclear antibodies (ANAs), but this has not been confirmed in patients, and particular HLA alleles associated with recurrent miscarriage have not been reported. (medscape.com)
In the case of systemic lupus erythematosus (SLE) and related illnesses, many of the HLA class II alleles are associated with the presence of specific autoantibodies or clinical phenotypes. (musculoskeletalkey.com)
Antibodies5
Anti-GBM antibodies are directed against an epitope located at the NC1 domain at the C-terminal of the alpha-3 chain of type IV collagen. (medscape.com)
When bound to the specific antigens in the kidneys and lungs, the antibodies initiate an inflammatory destruction of tissues by complement activation and recruitment of proinflammatory cells, leading to rapidly proliferative glomerulonephritis, often accompanied by pulmonary hemorrhage. (medscape.com)
Once the anti-GBM antibodies bind to the specific GBM antigen, complement is activated. (medscape.com)
Coexistence of ANCAs (mostly myeloperoxidase [MPO-ANCAs]) with anti-GBM antibodies is thought to occur when the renal involvement in ANCA vasculitis leads to the exposure of antigens from the basement membrane and the formation of anti-GBM antibodies. (medscape.com)
Anti-GBM antibodies interact with the GBM glycoproteins, almost exclusively the epitope of the noncollagenous domain (NC1) of the α3 chain of type IV collagen. (medscape.com)
Peptide5
The central goal of the work was to identify MHC class II peptide epitopes in such proteins, which exhibit a tumor-associated expression and which were already well-known target antigens of cytotoxic T cell reactions. (uni-goettingen.de)
Then, epitope candidates were characterized by establishing and analyzing peptide-specific T cell clones. (uni-goettingen.de)
Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. (novusbio.com)
The polymorphisms associated with the "shared epitope" are located on the α-helical rim (DRB1 chain) of the peptide-binding cleft, where they may interact with either the bound peptide antigen or the T cell receptor. (musculoskeletalkey.com)
Antigenic2
Because the antigenic epitope is hidden within the triple helix of the collagen, an environmental factor (eg, smoking, hydrocarbon exposure) presumably is required to unmask the cryptic antigen to the immune system. (medscape.com)
The allelic variations among different HLA molecules are a major factor accounting for differences in the types of antigenic peptides to which an individual responds or in the types of T cells that are used in an immune response. (musculoskeletalkey.com)
Peptides3
MHC class II candidate epitopes from these proteins were computer-assisted predicted and used as synthetic peptides for in vitro stimulation by human CD4+ T cells. (uni-goettingen.de)
Several class-II epitope candidates in SVN and PR3 were identified by examining human T cell responses to synthetic peptides. (uni-goettingen.de)
Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
Immune3
Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
Homocitrullination occurs in the tumor microenvironment and CD4-mediated immune responses to Hcit epitopes can target stressed tumor cells and provide a potent antitumor response in mouse models. (bmj.com)
The HLA molecules and their counterparts in rodents were subsequently shown to be directly responsible for immune response differences between individuals and for determining the likelihood of graft rejection. (musculoskeletalkey.com)
Lymphoma2
In this work, proteinase 3 (PR3) and survivin (SVN) were selected as candidate antigens associated with leukemia and lymphoma. (uni-goettingen.de)
Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. (novusbio.com)
Molecules4
In each individual, T cells are generally restricted to recognize antigens presented by the person's own HLA molecules. (musculoskeletalkey.com)
HLA class I molecules consist of a 45-kD α chain encoded within the MHC that is noncovalently associated with the 12-kD β 2 -microglobulin chain (encoded on chromosome 15). (musculoskeletalkey.com)
HLA class II molecules consist of noncovalently associated α (32 kD) and β (28 kD) chains, both of which are encoded within the MHC. (musculoskeletalkey.com)
HLA class I and class II molecules are cell surface glycoproteins, anchored to the membrane by hydrophobic transmembrane segments. (musculoskeletalkey.com)
Tumor4
Because the main effort has been to induce CTL responses, a logical way to enhance the potency of these tumor vaccines is to include CD4+ T cell epitopes for the same tumor antigen. (uni-goettingen.de)
Furthermore, the SVN10-specific T cells recognized SVN antigen from various tumor cell lines. (uni-goettingen.de)
Importantly, in vivo the homocitrullinated aldolase specific response was associated with efficient CD8 dependent antitumor therapy of the aggressive murine B16 tumor model indicating that this epitope is naturally presented in the tumor. (bmj.com)
In addition, the homocitrullinated aldolase epitope was also detected in human tumor samples. (bmj.com)
Potent1
Thus, the identification of the naturally processed SVN epitope, together with the previous finding of class I-restricted SVN epitopes, provide a basis for the combined application of class I- and II-restricted SVN epitopes to induce potent, long-term CD4+ and CD8+ T cell responses against various tumors. (uni-goettingen.de)
Avidity1
Moreover, the T cell clones were examined for MHC restriction patterns, immunophenotypes and epitope-MHC avidity. (uni-goettingen.de)
Naturally1
It was demonstrated that CD4+ T cells specific for the SVN10 epitope respond to naturally processed SVN proteins. (uni-goettingen.de)
Counterparts1
HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. (novusbio.com)
Human1
During this time, the perception of CD has changed from a rather uncommon enteropathy to a common multi- organ disease with a strong genetic predisposition that is associated mainly with human leukocyte antigen (HLA)-DQ2 and HLA-DQ8. (vdocuments.net)
Cells1
Experiments in animal models have demonstrated the importance of antigen-specific CD4+ T cells in the elimination of tumors. (uni-goettingen.de)
Cell surface1
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. (novusbio.com)
Antigenic epitope2
4. Ii-Key/HER-2/neu MHC class-II antigenic epitope vaccine peptide for breast cancer. (nih.gov)
Because the antigenic epitope is hidden within the triple helix of the collagen, an environmental factor (eg, smoking, hydrocarbon exposure) presumably is required to unmask the cryptic antigen to the immune system. (medscape.com)
Basement membrane1
Coexistence of ANCAs (mostly myeloperoxidase [MPO-ANCAs]) with anti-GBM antibodies is thought to occur when the renal involvement in ANCA vasculitis leads to the exposure of antigens from the basement membrane and the formation of anti-GBM antibodies. (medscape.com)
Vaccine2
Vaccine nonresponse is consistently associated with HLA-DRB1*0301 and *0401 in many populations. (medscape.com)
[ 10-12 ] The associations of HLA-DR7 (DRB1*07-) and HLA-DR3 (DRB1*0301) with persistent infection [ 13 , 14 ] are interesting because DRB1*0701 and DRB1*0301 have also been associated with failure to respond to HBsAg-based vaccine. (medscape.com)
Helper3
During a streptococcal infection, mature antigen-presenting cells such as B cells present the bacterial antigen to CD4+T cells which differentiate into helper T2 cells. (wikipedia.org)
[ 20 , 21 ] The ability to bind and present a wide range of epitopes is likely to generate a polyclonal and multispecific T-helper cell response, as seen in individuals who spontaneously eliminate the infection. (medscape.com)
15. Identification of HLA DR7-restricted epitopes from human telomerase reverse transcriptase recognized by CD4+ T-helper cells. (nih.gov)
Molecular1
11. Evaluation of pre-existent immunity in patients with primary breast cancer: molecular and cellular assays to quantify antigen-specific T lymphocytes in peripheral blood mononuclear cells. (nih.gov)
Cell4
[ 18 ] Furthermore, CD4 + T cell responses in patients who have recovered from HBV are greater in those who carry the HLA-DRB1*1302 allele. (medscape.com)
Furthermore, a broad range of potential T cell epitopes would reduce the opportunity for the virus to evade recognition through sequence variation. (medscape.com)
16. Tumor antigen-specific T-cell expansion is greatly facilitated by in vivo priming. (nih.gov)
In addition, we showed that DR5/DR7 APCs from celiac patients stimulated an effector CD4 + T cell response higher with respect to that induced by DR5/DR7 APCs from healthy subjects. (nih.gov)
Present1
The importance of this ability to present a wide range of epitopes is further underlined by the finding that individuals who are heterozygous at MHC class II loci are less likely to develop persistent HBV infection than homozygous individuals. (medscape.com)
Responses1
1. Proliferative and cytokine responses to class II HER-2/neu-associated peptides in breast cancer patients. (nih.gov)
Patients1
The DR5-DQ7/DR7-DQ2 genotype is very frequent among patients affected by celiac disease (CD), in Europe. (nih.gov)
Studies1
MHC class II association studies in persistent HBV infection are summarized in Table 1 . (medscape.com)
Association3
It is not yet clear what mechanism underlies the association of HLA-DRB1*1302 with spontaneous viral clearance. (medscape.com)
[ 23 , 24 ] Of these the most interesting finding has been the association of the class II allele DQB1*0301 and self-limiting HCV. (medscape.com)
Anti-GBM disease shows a strong association with HLA-DR2. (medscape.com)