• Several studies show that mesenchymal stem cells (MSCs) homing to tumors not only provide the microenvironment for tumor cells but also promote tumor growth and metastasis . (bvsalud.org)
  • Our data proved that the high concentration of IL15 in tumor microenvironment , which was mainly secreted by GCMSCs, may contribute to tumor cell metastasis and offer a new opportunity to develop effective therapeutics for intercepting tumor progression. (bvsalud.org)
  • Overexpression of Snail has been associated with breast cancer metastasis, although the specific role of Snail in the process remains unclear. (spandidos-publications.com)
  • Tumor cell motility is the hallmark of invasion and an essential step in metastasis. (spandidos-publications.com)
  • A number of reports have shown that RhoA expression is upregulated in a group of malignancies ( 13 ) and that the activity of RhoA is correlated with lymph node metastasis in colorectal cancer ( 14 ). (spandidos-publications.com)
  • In the present study, Snail and RhoA were observed to be overexpressed in breast cancer tissues compared with normal breast tissues and the expression of Snail and RhoA was associated with the differentiation grade and lymph node metastasis of breast cancer, respectively. (spandidos-publications.com)
  • We hypothesized that Snail, as a transcription factor, may promote breast cancer metastasis through the regulation of RhoA expression and activity. (spandidos-publications.com)
  • Understanding the molecular mechanisms of metastasis is a major step in designing effective cancer therapeutics. (jci.org)
  • Here we show that the T-box transcription factor Brachyury induces in tumor cells epithelial-mesenchymal transition (EMT), an important step in the progression of primary tumors toward metastasis. (jci.org)
  • Patients diagnosed with sarcomatoid clear cell renal cell carcinoma face a considerably worse prognosis due to an increased propensity for metastasis. (lu.se)
  • Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
  • Seventy percents of ovarian cancer patients at their first diagnosis were found to have ascites or distant metastasis. (biomedcentral.com)
  • Accordingly, the development of novel therapeutic agents to prevent or suppress ovarian cancer metastasis, has been considered as a potential strategy to improve the therapeutic effect of ovarian cancer patients. (biomedcentral.com)
  • The epithelial-to-mesenchymal changes in cell phenotype has been defined as the epithelial-mesenchymal transition (EMT), which plays a critical role in cancer metastasis [ 2 , 3 ]. (biomedcentral.com)
  • Cysteine-rich protein 61 (CCN1/Cyr61) has been implicated as an important mediator in the proliferation and metastasis of breast cancer. (molcells.org)
  • Pathway-based biomarkers identification may help elucidate the cellular signature of breast cancer metastasis in bone, further characterizing the etiology and promoting new therapeutic approaches. (degruyter.com)
  • A total of 61 DEGs, included 27 down-regulated and 34 up-regulated, were found to be significant in breast cancer bone metastasis. (degruyter.com)
  • DEGs Bcl3, ADGRG7, FABP4, IRF4, their regulating miRNAs and TFs have strong impact on proliferation and metastasis of breast cancer in bone tissues. (degruyter.com)
  • Breast cancer metastasis accounts for the majority of deaths from breast cancer. (iiarjournals.org)
  • Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. (iiarjournals.org)
  • Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
  • Additionally, a deeper understanding of the metastatic cascade in breast cancer will be critical for developing therapeutic interventions to combat breast cancer metastasis. (iiarjournals.org)
  • In this review, the current and novel methods for detection of breast cancer metastasis, as well as the mechanisms involved in metastasis and the treatment of breast cancer metastasis, are discussed. (iiarjournals.org)
  • The majority of deaths from breast cancer are not due to the primary tumor itself, but are the result of metastasis to other organs in the body ( 2 ). (iiarjournals.org)
  • Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
  • An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
  • Co-senior author Sendurai Mani, Ph.D ., assistant professor in MD Anderson's Department of Molecular Pathology and Co-Director of the Metastasis Research Center, is an expert on epithelial-to-mesenchymal transition (EMT). (weeksmd.com)
  • This change from stationary epithelial cells to the mobile mesenchymal stem cells is an important step in metastasis," Mani said. (weeksmd.com)
  • In cancer, invasion and metastasis are suggested to be induced by processes such as epithelial to mesenchymal transition (EMT), essentially shifting differentiated cells back to an early developmental programme. (databasefootball.com)
  • Metastasis of cancer cells involves a process called epithelial-mesenchymal transition (EMT), wherein epithelial cells undergo phenotypic changes to resemble mesenchymal cells -- stem cells capable of turning into different types of adult cells. (naturalnews.com)
  • Hence, the inhibition of EMT is now considered a promising strategy for stopping cancer metastasis. (naturalnews.com)
  • The researchers found that YGJDSJ can decrease the expression of proteins important for metastasis , making it a promising natural medicine for metastatic liver cancer. (naturalnews.com)
  • Euphorbia helioscopia (madwoman's milk or ze qi) -- Like L. lucidum , E. helioscopia can stop tumor growth , induce cell death, and inhibit the metastasis of HCC in vivo. (naturalnews.com)
  • These activities may be used for the prevention of liver cancer metastasis . (naturalnews.com)
  • concluded that c-Met is related to the development and metastasis of a number of malignancies, including breast cancer. (scite.ai)
  • Epithelial-mesenchymal transition (EMT) and most cancers stem cells (CSCs) play a vital function in metastasis of papillary thyroid most cancers (PTC). (aabioetica.org)
  • Additional mesenchymal marker vimentin is linked with metastasis and most cancers stem cell technology. (aabioetica.org)
  • For the majority of cancer patients, it's not the primary tumor that is deadly, but the spread or "metastasis" of cancer cells from the primary tumor to secondary locations throughout the body that is the problem. (sciencedaily.com)
  • That's why a major focus of contemporary cancer research is how to stop or fight metastasis. (sciencedaily.com)
  • Our team is hopeful that, because of the new findings, the substantial body of knowledge that has already been acquired on how to block EMT and reduce metastasis in experimental models may now begin to be applied to humans," said Georgia Tech graduate student Loukia Lili, co-author of the study. (sciencedaily.com)
  • Emerging evidence has demonstrated that WISP2 is critically involved in cell proliferation, migration, invasion and metastasis in cancers. (biomedcentral.com)
  • Thus, it is urgent to explore novel targets that may provide potential resolutions for metastasis in colorectal carcinoma cells. (biomedcentral.com)
  • Furthermore CDKL2 improved major tumor development and metastasis in a breast cancer xenograft model. (healthcarecoremeasures.com)
  • Cancer stem cells (CSCs) are a key driver of tumor formation and metastasis, but how they are affected by nanomaterial s is largely unknown. (cdc.gov)
  • Clinical characteristics and their influences on the survival of leptomeningeal metastasis derived from lung adenocarcinoma harboring epithelial growth factor receptor mutation]. (cdc.gov)
  • This predict the likelihood of a later recurrence, i.e., an indicator that unique longitudinal sample material was collected from each pa- allows risk assessment for breast cancer metastasis, would be tient between 0 and 36 mo after the primary operation. (lu.se)
  • Overexpression of Brachyury in human carcinoma cells induced changes characteristic of EMT, including upregulation of mesenchymal markers, downregulation of epithelial markers, and an increase in cell migration and invasion. (jci.org)
  • Conversely, inhibition of Brachyury resulted in downregulation of mesenchymal markers and loss of cell migration and invasion and diminished the ability of human tumor cells to form lung metastases in a xenograft model. (jci.org)
  • Moreover, BS inhibited migration and invasion and downregulated epithelial-mesenchymal transition (EMT) markers and AKT/GSK-3β signaling pathways. (nih.gov)
  • Using 6 biphasic clear cell renal cell carcinoma cases we show that sarcomatoid clear cell renal cell carcinoma shares characteristic markers associated with loss of von Hippel-Lindau tumor suppressor with conventional clear cell renal cell carcinoma and also exhibits a markedly higher proliferative index. (lu.se)
  • Furthermore the sarcomatoid elements demonstrate an enhanced expression of epithelial-mesenchymal transition related mesenchymal markers as compared with the clear cell renal cell carcinoma counterparts. (lu.se)
  • We further selected a representative case, clinically demonstrating direct overgrowth of the sarcomatoid component into the liver and colon, for extended immunohistochemical characterization, resulting in a further set of positive and negative epithelial-mesenchymal transition markers as well as pronounced transforming growth factor β positivity, indicating that sarcomatoid clear cell renal cell carcinoma may be associated to epithelial-mesenchymal transition. (lu.se)
  • Corresponding changes in RNA levels for key epithelial-mesenchymal transition markers were also seen. (lu.se)
  • 6 ) emphasized that the inclusion of serum tumor markers is an important factor in the postoperative monitoring of breast cancer patients ( 7 , 8 ). (iiarjournals.org)
  • This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
  • In the current project, the researchers hypothesized that the cell markers expressed on the surface of mesenchymal stem cells would also be expressed on the surface of breast cancer stem cells. (weeksmd.com)
  • Early researches on CSCs have been focused on verifying the existence of CSCs in certain types of cancer and finding molecular markers for isolation of CSCs. (bmbreports.org)
  • They identified EMT by observing cell morphological changes under a microscope, and by confirming the expression and phosphorylation of protein markers using Western Blot. (naturalnews.com)
  • The researchers reported that YGJDSJ treatment reversed the morphological changes (from epithelial to mesenchymal) induced by TGF- B 1 and the expression of EMT markers. (naturalnews.com)
  • EMT was determined by cell invasion assays using a transwell chamber and the expression changes of EMT-related markers were confirmed by RT-PCR, Western blotting, and immunofluorescence staining. (biomedcentral.com)
  • TG2 expression correlated with tumour advancement and expression of markers of epithelial-mesenchymal transition (EMT). (oncotarget.com)
  • Current methods of detecting CTCs mainly rely on immunological detection of protein markers on the tumor cells. (uh.edu)
  • mobile assays [1 20 21 using human being mammary gland epithelial cells (HMLE) a vintage EMT experimental model [13 14 22 In keeping with outcomes from the luciferase reporter assay inside our cDNA displays many under-studied kinase applicants and positive settings (FYN and MET) significantly up-regulated the manifestation of mesenchymal markers including vimentin fibronectin and N-cadherin in HMLE cells (Fig. (healthcarecoremeasures.com)
  • It has additionally been proven to take part in learning and storage in mice [28 30 Since HMLE cells include some Compact disc44high/Compact disc24low mesenchymal cells and exhibit measureable degrees of mesenchymal markers vimentin and N-cadherin we following examined if the detectable mesenchymal phenotypes could possibly be weakened through shRNA-mediated down-regulation of CDKL2 gene. (healthcarecoremeasures.com)
  • In addition, some selected tumor markers should be determined, such as alpha-fetoprotein (αFP) for hepatocellular carcinoma and germ cell tumors, beta-human chorionic gonadotropin (βHCG) for germ cell tumors, and prostate specific antigen (PSA) for prostate carcinoma. (medscape.com)
  • The WT1 protein mediates the mesenchymal-epithelial transition and differentiation during morphogenesis of the kidney and gonad by repressing genes that encode cell proliferation factors and by activating genes that encode markers of epithelial cell differentiation. (medscape.com)
  • Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
  • BS effectively inhibited the growth of PC cell lines by inhibiting proliferation, inducing G0/G1 phase arrest and apoptosis, suppressed the NF- kB activity, and increased expression of the protein Bax but decreased expression of the protein Bcl-2. (nih.gov)
  • HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
  • miRNAs and lncRNAs participate in the regulation of cell growth, proliferation, differentiation, migration and invasion by interacting with a number of signaling pathways ( 7 , 8 ). (spandidos-publications.com)
  • A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. (mdpi.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
  • HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
  • The breast cancer stem cells have the ability of infinite proliferation and multi-directional differentiation by themselves. (omicsonline.org)
  • Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. (biomedcentral.com)
  • Our previous study has proved GEN inhibits EGF-induced proliferation in colon cancer cells by promoting FOXO3 activity, targeting upstream the PI3K/Akt pathway [ 3 ]. (biomedcentral.com)
  • In this study, we demonstrated that GEN can inhibite proliferation and induce apoptosis of colon cancer cells by reversal of EMT via a Notch1/NF-κB/Slug/E-cadherin pathway. (biomedcentral.com)
  • Therefore, understanding the mechanisms underlying the invasion, proliferation, and cell transfer of breast cancer and the discovery of new therapeutic molecular targets are extremely important. (scite.ai)
  • Background Aquaporin 3 (AQP3) and Cellular-mesenchymal to epithelial transition factor (c-Met) are both overexpressed in human breast cancer and highly related to proliferation and migration. (scite.ai)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
  • Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
  • Therefore, we aim to explore the effects and the potential mechanism of WISP2 on proliferation and motility and invasion of ESCC cells. (biomedcentral.com)
  • Cell proliferation was detected by MTT assay and apoptosis was measured by FACS in ESCC cells after WISP2 downregulation and overexpression. (biomedcentral.com)
  • One study further identified that WISP2 expression was enhanced by serum and correlated with serum-induced cell proliferation in breast cancer cells, demonstrating that WISP2 could enhance cell proliferation in breast cancer [ 8 ]. (biomedcentral.com)
  • SDC-1 overexpression inhibited cell proliferation via suppressing CyclinD1 and c-Myc expression, meanwhile stimulated cell apoptosis via increasing the expression levels of B-cell lymphoma-2-associated x (Bax) and Cleaved-Caspase-3. (biomedcentral.com)
  • MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/β-catenin pathway by negative regulation of Smad7. (cancerindex.org)
  • Analysis of chemically distinct conjugates, resistance models, and biomarkers implicates a unique mode of action, with mitotic arrest and apoptosis of malignant epithelial cells coupled to disruption of fibroblastic and vascular structures. (aacrjournals.org)
  • EMT converts polarized and immotile epithelial cells to motile mesenchymal cells, accompanied by loss of cell-cell adhesion, planar and apical-basal polarity, and acquired mesenchymal features, including motility, invasiveness and enhanced resistance to apoptosis [ 4 ]. (biomedcentral.com)
  • Bardoxolone methyl induces apoptosis and autophagy and inhibits epithelial-to-mesenchymal transition and stemness in esophageal squamous cancer cells. (stembook.org)
  • Induction of apoptosis and autophagy via sirtuin1- and PI3K/Akt/mTOR-mediated pathways by plumbagin in human prostate cancer cells. (stembook.org)
  • The investigational Aurora kinase A inhibitor alisertib (MLN8237) induces cell cycle G2/M arrest, apoptosis, and autophagy via p38 MAPK and Akt/mTOR signaling pathways in human breast cancer cells. (stembook.org)
  • The pan-inhibitor of Aurora kinases danusertib induces apoptosis and autophagy and suppresses epithelial-to-mesenchymal transition in human breast cancer cells. (stembook.org)
  • Tumor necrosis factor-α (TNF-α) has been considered stimulated the EMT in several kinds of cancer cells which is a function that contrasts with its more established role in inducing apoptosis [ 7 , 12 , 13 ]. (biomedcentral.com)
  • Apoptosis, which is a major way of programmed cell death, has been known to all plays an important role in the regulation of tissue development and homeostasis [ 17 ]. (biomedcentral.com)
  • In recent years, the role of EMT in cell apoptosis has received considerable attention [ 18 , 19 ]. (biomedcentral.com)
  • Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • WISP2 overexpression inhibited cell growth and induced cell apoptosis, suppressed cell migration and invasion in ESCC cells. (biomedcentral.com)
  • Next, SW480, SW620 and LOVO cell viability, apoptosis, migration and adhesion were assessed to explore the effects of exogenous overexpressed SDC-1 on colorectal carcinoma. (biomedcentral.com)
  • In this paper, we verified the mRNA and protein expression of SDC-1 in human colorectal carcinoma tissues and focused on the biological cellular effects of SDC-1 on human colorectal carcinoma cell lines (SW620, SW480 and LOVO), by evaluating cell viability, apoptosis, migration and adhesion. (biomedcentral.com)
  • The current results indicated that B[a]P seems to induce EMT in lung cancer cells, and it also may drive disease progression in patients with lung cancer. (nih.gov)
  • Our study aims to investigate the role of gastric cancer MSCs (GCMSCs)-derived IL15 during GC progression. (bvsalud.org)
  • We also determined that the percentage of human lung tumor tissues positive for Brachyury expression increased with the stage of the tumor, indicating a potential association between Brachyury and tumor progression. (jci.org)
  • The selective expression of Brachyury in tumor cells and its role in EMT and cancer progression suggest that Brachyury may be an attractive target for antitumor therapies. (jci.org)
  • Studies [ 13 - 15 ] have shown that miR-149 plays an important role in many cancers, although its regulatory role in the FOXC1-mediated progression of gastric cancer remains unclear. (hindawi.com)
  • The microenvironment is fundamental in attracting tumor cells to the bone as well as promoting tumor progression. (degruyter.com)
  • These differences affect the progression of cancer and how it responds to therapeutic agents and their ultimate efficacy. (degruyter.com)
  • Andreeff and colleagues are refining the drug as a potential targeted therapy for breast cancer stem cells, which are thought to be crucial to therapy resistance, disease progression and spread to other organs. (weeksmd.com)
  • Epithelial mesenchymal transition (EMT) is an important process during tumor progression which affects critical steps of morphogenesis by interconverting epithelial cell types into cells with mesenchymal attributes [ 11 ]. (biomedcentral.com)
  • The extracellular matrix (ECM) is a crucial component of the tumor microenvironment involved in numerous cellular processes that contribute to cancer progression. (scite.ai)
  • This conundrum brings to question whether cancer ST6GAL1 overexpression is beneficial or ultimately detrimental to long-term patient outcomes and highlights the incomplete mechanistic understanding of how ST6GAL1 is involved in cancer progression. (nature.com)
  • It is within the cell-autonomous context that ST6GAL1 involvement in cancer progression has been interpreted. (nature.com)
  • Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
  • However, the function of WISP2 in esophageal squamous cell carcinoma (ESCC) is largely unclear. (biomedcentral.com)
  • PRGOMET Z, ANDERSSON T and LINDBERG P. Higher expression of WNT5A in oral squamous cell carcinoma compared to dysplasia and oral mucosa with a normal appearance. (lu.se)
  • Rarely, mucinous differentiation or squamous cell carcinoma is seen. (medscape.com)
  • Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a protein that in humans is encoded by the GDF2 gene. (wikipedia.org)
  • GDF2 induces the differentiation of mesenchymal stem cells (MSCs) to an osteoblast lineage. (wikipedia.org)
  • GDF2 activate JNK in osteogenic differentiation of mesenchymal progenitor cells (MPCs). (wikipedia.org)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • CSCs take charge of a cancer hierarchy, harboring stem cell-like properties involving self-renewal and aberrant differentiation potential. (bmbreports.org)
  • c-Met binds to HGF, resulting in the autophosphorylation and overexpression of c-Met, and this interaction participates in embryonic development, wound repair, tissue regeneration, cell differentiation, etc. (scite.ai)
  • MEHDAWI LM, PRASAD CP, EHRNSTRÖM R, ANDERSSON T and SJÖLANDER A. Non-canonical WNT5A signaling up-regulates the expression of the tumor suppressor 15-PGDH via JNK1 and induces differentiation of colon cancer cells. (lu.se)
  • in turn, the malignant epithelial cells can undergo reversible epithelial-mesenchymal transitions ( 1 - 5 ). (aacrjournals.org)
  • Mani and colleagues at MIT showed that epithelial cells can be induced to take on stem cell properties by forcing them to undergo EMT. (weeksmd.com)
  • Andreeff and Mani in 2010 discovered that human mammary epithelial cells that undergo epithelial-to-mesenchymal transition act similarly to human bone-marrow-derived mesenchymal stem cells. (weeksmd.com)
  • Tumour cells undergo a huge loss of DNA methylation across the genome, and the specific loss of silencing at viral DNA sequences is a hallmark of many cancers. (databasefootball.com)
  • Previous lab studies suggest that metastasizing cancer cells undergo a major molecular change when they leave the primary tumor -- a process called epithelial-to-mesenchymal transition (EMT). (sciencedaily.com)
  • First, when tumor cells get into the blood, they intend to undergo a biological change called epithelial-mesenchymal transition (EMT). (uh.edu)
  • Recombinant protein encompassing a sequence within the center region of human Vimentin. (genetex.com)
  • Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. (genetex.com)
  • Non-transfected (-) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. (genetex.com)
  • Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis. (genetex.com)
  • Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. (genetex.com)
  • Solanum nigrum (black nightshade or long kui) -- The active component of this herb, a -solanine, can suppress EMT by decreasing the expression of vimentin , a mesenchymal protein marker. (naturalnews.com)
  • Genistein was found can induce the reversal of EMT in prostate cancer cells by an upregulated expression of epithelial marker E-cadherin and the loss of expression of mesenchymal marker vimentin [ 22 ]. (biomedcentral.com)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • Indeed prostate malignancy epithelial cell collection PC3 and pancreatic malignancy epithelial cell collection Mubritinib SU86.86 showed increased vimentin expression and enhanced migration ability with CDKL2 ectopic expression (Fig. (healthcarecoremeasures.com)
  • We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
  • lncRNA‑NEF overexpression inhibited, while miRNA‑21 overexpression promoted, migration and invasion of osteosarcoma cell lines in vitro. (spandidos-publications.com)
  • miRNA‑21 overexpression partially compensated the inhibitory effects of lncRNA‑NEF overexpression on osteosarcoma cell migration and invasion. (spandidos-publications.com)
  • In the present study, we report that baicalein significantly inhibits the expression of Cyr61 and migration and invasion of MDA-MB231 human breast cancer cells. (molcells.org)
  • From Local Invasion to Metastatic Cancer. (iiarjournals.org)
  • Good models for cancer invasion in humans are rare, but remarkably, the human placenta presents an excellent model for cellular invasion and may help in the discovery of new invasion-associated genes. (databasefootball.com)
  • Our work suggests that similar to the permissive state of unsilenced viral DNA in the placenta, the unsilencing of viral DNA in cancer is important for cancer cell invasion. (databasefootball.com)
  • The aim of our current research at the University of Otago (in Dunedin, New Zealand) is to generate a catalog of recently evolved placental RDGs and determine whether these genes play a role in driving cancer invasion. (databasefootball.com)
  • This will determine whether essential placental genes are activated in cancer cells, and if so, whether they drive cancer cell invasion. (databasefootball.com)
  • They also used the scratch assay to measure cell migration and a commercial kit to detect cell adhesion and invasion. (naturalnews.com)
  • YGJDSJ also inhibited TGF- B 1 up-regulated Smad3 phosphorylation and Snail expression, as well as TGF- B 1-induced cell adhesion, migration, and invasion. (naturalnews.com)
  • GEN was also suggested can inhibit cell migration and invasion in both AsPC-1 and Notch-1-over-expressed AsPC-1 cells as Notch-1 could play a key role in the regulation of EMT [ 23 ]. (biomedcentral.com)
  • These discoveries may help us comprehend the mechanisms underlying breast cancer development and invasion and offer another possibility for targeted treatment of breast cancer. (scite.ai)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)
  • Results revealed that hypoxia exposure upregulated Nodal expression and activated Nodal signaling in melanoma cancer cell A375, contributed to maintain stemness and promotes malignancy potential, including invasion, sphere formation and dacarbazine resistance. (cusabio.com)
  • Cell migration and invasion were analyzed by wound healing assay and transwell migration assay, respectively. (biomedcentral.com)
  • PRASAD CP, SÖDERGREN K and ANDERSSON T. Reduced production and uptake of lactate are essential for the ability of WNT5A signaling to inhibit breast cancer cell migration and invasion. (lu.se)
  • PRASAD CP, CHAURASIYA SK, GUILMAIN W, and ANDERSSON T. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition. (lu.se)
  • Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion. (lu.se)
  • The effects of IL15 secreted by GCMSCs on GC development were evaluated by detecting the stemness, epithelial-mesenchymal transition (EMT), and migration abilities of GC cell lines . (bvsalud.org)
  • We found that IL15 derived from GCMSCs enhanced stemness, induced EMT and promoted migration of GC cell lines . (bvsalud.org)
  • These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
  • The effects on cancer cell migration and motility were evaluated by wound-healing and transwell assays. (biomedcentral.com)
  • Genistein inhibited cell migration at 200 μmol/L. Genistein reversed the EMT of colon cancer cells by upregulation of E-cadherin and downregulation of N-cadherin, accompanied by the suppression of EMT related makers, such as Snail2/slug, ZEB1, ZEB2, FOXC1, FOXC2 and TWIST1. (biomedcentral.com)
  • The present study demonstrated that genistein suppressed the migration of colon cancer cells by reversal the EMT via suppressing the Notch1/NF-κB/slug/E-cadherin pathway. (biomedcentral.com)
  • Additionally, cell migration was examined with a wound scratch assay. (scite.ai)
  • Additionally, the wound scratch assay showed that AQP3-specific siRNA or c-Met-specific siRNA significantly inhibited breast cancer cell migration, which was promoted by hHGF. (scite.ai)
  • Additionally, SDC-1 overexpression restrained cell migration via inhibiting the protein expression of matrix metallopeptidase 9 (MMP-9), and elicited cell adhesion through increasing intercellular cell adhesion molecule-1 (ICAM-1). (biomedcentral.com)
  • In general, the evidence from this study suggested that SDC-1 suppressed cell growth, migration through blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. (biomedcentral.com)
  • CDKL2 is certainly a book promoter for EMT and stem cell-like phenotypes Elevated migration is a vintage feature of EMT cells. (healthcarecoremeasures.com)
  • MOHAPATRA P, PRASAD CP and ANDERSSON T. Combination therapy targeting the elevated interleukin-6 level reduces invasive migration of BRAF inhibitor-resistant melanoma cells. (lu.se)
  • Plus, research in 2019 suggested that K3 could suppress some activities of colorectal cancer cells. (greatist.com)
  • Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). (cancerindex.org)
  • The incidence rate of gastric cancer is fifth in the world, and the mortality rate is third, second only to lung cancer and colorectal cancer [ 1 ]. (hindawi.com)
  • An objective of this study was to investigate the role of SDC-1 in colorectal carcinoma cells. (biomedcentral.com)
  • SDC-1 mRNA and protein levels were down-regulated in human colorectal carcinoma tissues. (biomedcentral.com)
  • Colorectal carcinoma is generally unrecognized with symptomless in the early stage or is seen with ordinary symptoms in cancer metaphase, such as bloating and indigestion. (biomedcentral.com)
  • However, there is no data on the possible role of SDC-1 in human colorectal carcinoma. (biomedcentral.com)
  • Unveiling the metal mutation nexus: Exploring the genomic impacts of heavy metal exposure in lung adenocarcinoma and colorectal cancer. (cdc.gov)
  • Real-time PCR was performed for Brachyury on lung tumor tissue cDNA from 80 lung cancer patients of the indicated stages of disease. (jci.org)
  • Background: Vestigial like family member 3 (VGLL3) is associated with the prognosis of epithelial ovarian cancer and soft tissue sarcoma, but its role in gastric cancer (GC) is unclear. (researchgate.net)
  • The epithelial-mesenchymal transition (EMT) is an essential process in multiple biochemical changes, including embryonic development, tissue remodeling and wound healing. (molcells.org)
  • In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. (frontiersin.org)
  • Another suggestion is to have intensive postoperative follow-up which includes consultations every 4-6 months, physical examination and evaluation of serum carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and breast cancer-associated antigen 115 D8/DF3 (CA15.3), at each visit. (iiarjournals.org)
  • Cancer cells invade the surrounding tissue, making many cancers difficult to isolate and treat. (databasefootball.com)
  • Curiously, the placenta - a healthy tissue - has evolved in humans and some primates so that certain retrotransposons are allowed to escape epigenetic silencing. (databasefootball.com)
  • Here, our study demonstrated that AQP3 and c-Met were overexpressed in 52.5% and 83.1% of breast cancer tissue specimens, respectively. (scite.ai)
  • Methods We used immunohistochemistry to determine the expression of AQP3 and c-Met in breast cancer tissue specimens and analysed whether there was any correlation between the two molecules. (scite.ai)
  • Results Based on the immunohistochemical results combined with the clinicopathological data of 59 breast cancer patients, c-Met exhibited a significant correlation with AQP3 in breast cancer tissue. (scite.ai)
  • This Human NODAL ELISA Kit was designed for the quantitative measurement of Human NODAL protein in serum, plasma, tissue homogenates. (cusabio.com)
  • Since EMT has also been found in epithelial cell of other tissue types we suspected that CDKL2 plays a Mubritinib similar role in other epithelial cells. (healthcarecoremeasures.com)
  • β-sitosterol (BS), a major bioactive constituent present in plants, has shown potent anti-cancer activity against many human cancer cells, but its activity in pancreatic cancer (PC) cells has rarely been reported. (nih.gov)
  • Alisertib induces cell cycle arrest and autophagy and suppresses epithelial-to-mesenchymal transition involving PI3K/Akt/mTOR and sirtuin 1-mediated signaling pathways in human pancreatic cancer cells. (stembook.org)
  • Moreover, IL15 upregulated Tregs ratio through activation of STAT5 in CD4+ T cells was accompanied by elevated expression of programmed cell death protein -1 (PD-1). (bvsalud.org)
  • Snail is also an upstream protein of metalloproteinase-2 ( 9 ), which is a mesenchymal marker sufficient to trigger EMT in vivo ( 10 ). (spandidos-publications.com)
  • The Smad7 protein is negative regulator of the TGF-β signaling pathway, which is upregulated in patients with breast cancer. (cancerindex.org)
  • In addition to protein-coding genes, the human genome contains a large number of genes which are transcribed to non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) ( 7 , 8 ). (spandidos-publications.com)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
  • Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
  • Breast cancer stem cells wear a cell surface protein that is part nametag and part bull's eye, identifying them as potent tumor-generating cells and flagging their vulnerability to a drug, researchers at The University of Texas MD Anderson Cancer Center report online in Journal of Clinical Investigation . (weeksmd.com)
  • The marker is the cell surface protein ganglioside GD2. (weeksmd.com)
  • Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
  • Carcinosarcoma of the uterus is a biphasic neoplasm composed of malignant epithelial and mesenchymal elements. (medscape.com)
  • Carcinosarcomas comprise an admixture of histologically malignant epithelial and mesenchymal components, as depicted in the micrograph below. (medscape.com)
  • Using a physiologically relevant exposure model (long-term/low-dose) with system validation using a human carcinogen, asbestos, we demonstrated that single-walled carbon nanotube s, multi-walled carbon nanotube s, ultrafine carbon black, and crocidolite asbestos induced particle-specific anchorage-independent colony formation, DNA-strand breaks, and p53 downregulation, indicating the genotoxicity and carcinogenic potential of CNMs. (cdc.gov)
  • Primary breast cancer tumors may be removed or irradiated relatively simply, but distant metastases are difficult to treat. (spandidos-publications.com)
  • Chemotherapy, radiation therapy, and surgical removal of the tumors are standard methods implemented to cure breast cancers. (degruyter.com)
  • Recent research demonstrates that tumors originate from the Cancer S tem Cells (CSCs) or Tumor Initiated Cells (TIC) [ 1 ], which are called breast cancer stem cells in breast cancer. (omicsonline.org)
  • It's been difficult to identify cancer stem cells in solid tumors," Andreeff said. (weeksmd.com)
  • Triptolide stymied cancer growth in cell line experiments and resulted in smaller tumors and prolonged survival in mouse experiments. (weeksmd.com)
  • Research in several types of cancer has shown cancer stem cells are a small subpopulation of cancer cells that are capable of long-term self-renewal and generation of new tumors. (weeksmd.com)
  • Andreeff has shown these mobile mesenchymal stem cells home to wounds, including tumors, making them potential carriers of cancer therapy. (weeksmd.com)
  • Form five times as many tumors when 10 cells of each type are transplanted into mice. (weeksmd.com)
  • An analysis of 12 human breast cancer tumors found an even higher correlation of 95.5 percent between GD2+ cells and CD44 high/CD24 low status. (weeksmd.com)
  • Curcuma wenyujin (yu jin) -- This species of turmeric has an active compound called B -elemene, which can reverse the malignancy of human glioblastoma or brain tumors. (naturalnews.com)
  • Oral cancer is one of the most prevalent malignant tumors worldwide. (jcancer.org)
  • The findings suggest that doctors should treat patients with a combination of drugs: those that kill cancer cells in primary tumors and drugs that target the unique characteristics of cancer cells spreading through the body. (sciencedaily.com)
  • Circulating tumor cells, which are detached from primary tumors to enter the bloodstream, are particularly hard to detect. (uh.edu)
  • CTCs, like the tumors they are originally detached from, lack unique and well-defined universal biomarkers highlighting the challenges of detection specificity," reports Dr. Shaun Zhang, M.D. Anderson Professor of biology and biochemistry and director of the Center for Nuclear Receptors and Cell Signaling. (uh.edu)
  • Although the primary importance of CTCs is their ability to seed metastatic tumors, their constant presence in the blood have made their detection an attractive alternative to the traditional biopsy for clinical applications such as cancer screening, therapy evaluation and disease prognosis. (uh.edu)
  • Cancers of unknown primary origin are defined as histologically confirmed metastatic tumors for which no known primary site has been identified following thorough physical examination and laboratory and imaging diagnostic tests. (medscape.com)
  • Furthermore, we found Brachyury to be overexpressed in various human tumor tissues and tumor cell lines compared with normal tissues. (jci.org)
  • Brachyury expression in human lung tumor tissues. (jci.org)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • Twenty pairs of gastric cancer and adjacent nontumor tissues, which were not subjected to radiotherapy or chemotherapy, were obtained from the First Affiliated Hospital of Xi'an Jiaotong University. (hindawi.com)
  • MIR-34A has its own transcript and is expressed at higher levels than MIR-34B/C in most tissues, and this expression could be dysregulated in multiple diseases, especially in cancers [ 11 ]. (hindawi.com)
  • Cancer stem cells are similar to normal stem cells that renew specialized tissues. (weeksmd.com)
  • In early pregnancy, placental cells invade into the uterine wall to establish a blood supply for the fetus, just as cancer cells invade into surrounding tissues and initiate angiogenesis. (databasefootball.com)
  • Retrotransposons comprise 42% of the human genome, and due to their potentially destructive 'viral' nature, they are silenced (turned off) in healthy tissues so that the cells can behave normally. (databasefootball.com)
  • We and others have documented the specific unsilenced (unmethylated) state of retrotransposons in the placenta and their silenced (methylated) state in other human tissues. (databasefootball.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Gastric cancer is one of the most common cancers in the world. (hindawi.com)
  • The aim of this study was to investigate the function of miRNA-149 and FOXC1 in gastric cancer. (hindawi.com)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • The MTT, colony formation, flow cytometry, wound healing, transwell, and western blotting were performed to examine the function of miRNA-149 and FOXC1 in the development of gastric cancer. (hindawi.com)
  • miRNA-149 inhibits the biological behavior of gastric cancer by targeting FOXC1, providing a promising target in the treatment of human gastric cancer. (hindawi.com)
  • Gastric cancer is a highly recurrent malignancy. (hindawi.com)
  • The detection rate of early gastric cancer is low and lacks specificity. (hindawi.com)
  • In light of the advancements in tumor biology and molecular technology, further research into the molecular mechanism involved in gastric cancer and identification of biomarkers with high specificity and good targeting [ 3 ] is of great significance for the diagnosis and treatment of this condition. (hindawi.com)
  • Several studies have confirmed that miRNA can be used as an oncogene or tumor suppressor gene to regulate the occurrence and development of gastric cancer [ 6 ]. (hindawi.com)
  • Therefore, investigating the expression and regulation of miRNAs may elucidate the mechanism of gastric cancer. (hindawi.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
  • The overarching aim of the Epigenetics common cause of gastric cancer, Pan-cancer genoMe and Group (EGE) is to advance the which is the third most common cause tranScriPtoMe analySiS and understanding of the role of epigenetic of cancer-related deaths worldwide. (who.int)
  • MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling. (nih.gov)
  • 20 μg/mL, P = 0.001 vs. control group) and mesenchymal marker upregulation, e.g. (biomedcentral.com)
  • J. Experimental & Clinical Cancer Research 35(1):144 ( DOI 10.1186/s13046-016-0421-0), 2016. (lu.se)
  • Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. (hindawi.com)
  • Breast cancer metastases are most commonly found in bone, an indication of poor prognosis. (degruyter.com)
  • Metastatic recurrence in breast cancer is associated with poor prognosis and is a leading cause of cancer related death in women worldwide [ 2 ]. (degruyter.com)
  • Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
  • However, esophageal cancer patients often exhibit the onset of symptoms at diagnosis, which leads to poor prognosis. (biomedcentral.com)
  • The risk of distant recurrence in breast cancer patients is difficult to prognostic indices, such as the Nottingham Prognostic Index (6, 7), assess with current clinical and histopathological parameters, and have proven valuable in identifying patients with poor prognosis. (lu.se)
  • Guide to Signal Pathways in Immune Cells. (iiarjournals.org)
  • An elaborate network of signaling pathways orchestrates the communication between cancer cells and the surrounding stroma. (degruyter.com)
  • In this study, we explore different signaling pathways and associated molecular mechanisms of underlying primary breast cancer development, along with the formation of metastases in the bone. (degruyter.com)
  • exposures and lifestyle alter critical advances as well as the uniqueness and These developments have also led to molecular pathways and promote cancer strengths of IARC. (who.int)
  • orthogonal exPeriMental changes and pathways induced by EGE investigated the impact of aSSeSSMent of ePigenetic environmental factors in cancer causation, both current H. pylori infection and driver geneS and their link to underpinning studies of etiology e , pigenetic memory of past (eradicated) environMental carcinogenS carcinogen evaluation, and prevention. (who.int)
  • Although no apparent morphologic changes were observed, the B[a]P-exposed A549 cells exhibited enhanced proliferative activity in 1% bovine serum that contained medium, and dramatic changes in expression levels were observed in a large number of genes. (nih.gov)
  • Comparing gene expression between GD2+ cells and CD44 high/CD24 low cells revealed 100 percent correlation in the expression of 231 genes. (weeksmd.com)
  • Distributed among large numbers of retrotransposons in the human genome are a relatively small number of recently evolved, epigenetically unsilenced genes called "retrotransposon-derived genes" (referred to henceforth as RDGs). (databasefootball.com)
  • Interestingly, the evolutionary conservation of certain RDGs in the placentas of humans and some primates suggests that these new genes may contribute to the highly invasive phenotype of placentas in these species. (databasefootball.com)
  • Prognostic value of genes related to cancer-associated fibroblasts in lung adenocarcinoma. (cdc.gov)
  • Specifically, ERV-K members were originally identified due to their similarity to the Mouse Mammary Tumor Virus (MMTV) and are subdivided into ten so-called human MMTV-like clades (HML1-10) ( 2 , 3 ). (frontiersin.org)
  • These results suggest that CDKL2's role on EMT regulation is not limited to HMLE mammary gland epithelial cells. (healthcarecoremeasures.com)
  • We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)
  • The results from this prevali- the field of cancer biomarkers (11-13), and that approach was dation study showed that patients could be classified into high- adopted here to define predictive serum biomarkers associated versus low-risk groups for developing metastatic breast cancer with tumor relapse in breast cancer patients. (lu.se)
  • To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
  • The tumor cell mass exerts mechanical pressure that can contribute to bone pain. (degruyter.com)
  • Thus, the ECM works as a reservoir for bioactive molecules that modulate tumor cell behavior. (scite.ai)
  • The impact of this non-canonical extrinsic mechanism of ST6GAL1 on tumor cell pathobiology is not known. (nature.com)
  • We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
  • Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which is confirmed to participate in several tumor cell biological processes. (biomedcentral.com)
  • To investigate the effects and mechanisms of genistein on EMT of colon cancer cells, HT-29 cells were used and treated by genistein and TNF-α in this paper. (biomedcentral.com)
  • In vitro data has shown that GEN can inhibit the growth of several colon cancer cells [ 8 ], while its particular effects on cancer cells and the mechanisms involved remain unknown [ 9 , 10 ]. (biomedcentral.com)
  • The present study investigated the effects of different carbon-based nanomaterial s (CNMs) on neoplastic and CSC-like transformation of human small airway epithelial cells and determined the underlying mechanisms. (cdc.gov)
  • While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown. (biomedcentral.com)
  • However, current knowledge of GEN in regulating EMT of colon cancer cells is limited, and more detailed investigations of its function and mechanism are required. (biomedcentral.com)
  • Oral cancer is a common malignancy of the head and neck region [ 1 ], with more than 350,000 new cases and 177,000 deaths annually recorded worldwide [ 2 ]. (jcancer.org)
  • Approximately 8% of clear cell renal cell carcinoma cases contain regions of radically different morphology, demonstrating a mesenchymal appearance histologically resembling sarcomas. (lu.se)
  • Not for any clinical, therapeutic, or diagnostic use in humans or animals. (genetex.com)
  • ERV-K env is overexpressed and implicated as a therapeutic target for breast cancer, however studies in RCC are limited. (frontiersin.org)
  • If we determine how cancer cells become invasive, then we may be able to develop new therapeutic or diagnostic opportunities. (databasefootball.com)
  • According to recent findings, especially targeting cancer stem cells (CSCs) is the most promising therapeutic strategy. (bmbreports.org)
  • Silibinin has been reported to exert therapeutic effects in various cancer models. (jcancer.org)
  • Our data suggests that TG2 could hold both prognostic and therapeutic significance in colon cancer. (oncotarget.com)
  • MANCHANDA M, MOHAPATRA P and ANDERSSON T. Wnt5a as a therapeutic target in breast cancer. (lu.se)
  • Using miRNA-based classification and identification of cancers of unknown origin can pave the way to the use of more personalized and targeted therapeutic strategies. (medscape.com)
  • Needlessly to say HMLE-CDKL2 demonstrated higher migratory ability than vector control cells in Boyden chamber assay (Fig. (healthcarecoremeasures.com)
  • lncRNA‑NEF overexpression mediated the inhibition of miRNA‑21 expression in osteosarcoma cell lines, while miRNA‑21 overexpression did not significantly affect the expression of lncRNA‑NEF. (spandidos-publications.com)
  • As an oncogenic miRNA, miRNA-21 promotes tumor development in different types of cancer, including osteosarcoma ( 11 ). (spandidos-publications.com)
  • A 2020 study found that K3 has "potent anticancer activity" for some cancers of the breast, kidneys, liver, prostate, skin, and cervix. (greatist.com)
  • Prostate Cancer Screening. (iiarjournals.org)
  • CANESIN G, EVANS-AXELSSON S, HELLSTEN R, KRZYZANOWSKA A, PRASAD CP, BJARTELL A and ANDERSSON T. Treatment with the Wnt5a-mimicking peptide Foxy5 effectively reduces the metastatic spread of Wnt5a-low prostate cancer cells in an orthotopic mouse model. (lu.se)
  • In Sweden, there has been a steady prostate-specific antigen (PSA) velocity for the diagnosis of pros- decrease in age-standardized breast cancer mortality in women up tate cancer (15). (lu.se)
  • EMT not only increases the motility of cancer cells, but it also makes them more invasive. (naturalnews.com)
  • One of the most forceful underlying reasons is a "cancer heterogeneity" due to "CSC plasticity" A comprehensive understanding of CSC-derived heterogeneity will provide novel insights into the establishment of efficient targeting strategies to eliminate CSCs. (bmbreports.org)
  • For the past 20 years, plenty of studies have suggested that only a small subpopulation of the cancer cells with tumor-initiating capability is the core origin of the tumorigenesis and the subset of cancer cells was named cancer stem cells (CSCs). (bmbreports.org)
  • As it can be inferred from its nomenclature, CSCs share several features of normal stem cells. (bmbreports.org)
  • Resistance to the conventional anti-cancer therapies is a characteristic of CSCs which is most important from a clinical point of view. (bmbreports.org)
  • They found that GD2 expression, one such mesenchymal stem cell marker, divided the breast cancer cell lines into two distinct groups: about 4.5 percent of cells were GD2-positive and about 92.7 percent were GD2-negative. (weeksmd.com)
  • β-Catenin and TG2 was also upregulated in SW620 spheroid cells enriched with cancer stem cell marker CD44 and TG2 inhibition/knockdown reduced the spheroid forming potential of SW620 cells. (oncotarget.com)
  • EMT has been associated with acquisition of stem cell-like properties including expression of the putative breast cancer stem cell (CSC) marker Mubritinib CD44high/CD24low [13 27 CDKL2 ZAK FYN and MET promoted a 8-16 fold increase in the CD44high/CD24low subpopulation in HMLE cells compared to GFP control (Fig. (healthcarecoremeasures.com)
  • Physique 2 CDKL2-transduced cells show both EMT and stem cell-like phenotypes As a further validation for its role in promoting stem cell-like phenotypes CDKL2 induced a clear increase in the ability to form mammosphere an measure of stemness [31] (~8 fold increase in sphere figures and ~2 fold increase in sphere diameters) relative to Mubritinib vector control cells (Fig. (healthcarecoremeasures.com)
  • Acquisition of cancer stem cell-like properties in human small airway epithelial cells after a long-term exposure to carbon nanomaterial s. (cdc.gov)
  • At the end of 2023, researchers at Lund Stem Cell Center received funding amounting to SEK 77.5 million from the Swedish Research Council, the Swedish Cancer Society, and the Swedish Childhood Cancer Fund. (lu.se)
  • At Lund Stem Cell Center, researchers are using innovative approaches to advance the field, offering exciting possibilities for the future. (lu.se)
  • We hope that this new method will foster a culture of proactive health management and shift the paradigm towards preventive care, in addition to paving the way for more effective treatments," says Sandra Linstedt, research group leader at Lund Stem Cell Center. (lu.se)
  • Cells from the bronchioloalveolar carcinoma cell line A549 were exposed to benzo(a)pyrene (B[a]P) for 24 weeks, and morphology, proliferative activity, and gene expression profiles were analyzed. (nih.gov)
  • Augmented expression of HEY1 increase the mineralization of the cells. (wikipedia.org)
  • In cell type with low expression level of ActRIIB, GDF2 might still signal due to its affinity to ALK1, then form complex with type II receptors. (wikipedia.org)
  • Model based on GA and DNN for prediction of mRNA-Smad7 expression regulated by miRNAs in breast cancer. (cancerindex.org)
  • Approximately 3% of the human genome codes for miRNA sequences, which can suppress gene expression by mediating translational repression [ 5 ]. (hindawi.com)
  • Altered gene expression in these nontransformed stromal cells provides potential targets for therapy. (aacrjournals.org)
  • The active, un-phosphorylated form of GSK3β maintains both (i) epithelial phenotypes by inhibiting the expression and stabilization of Snail and Slug and (ii) high E-cadherin expression ( Boble and Woodgett, 2007 ) in resting epithelial cells. (molcells.org)
  • ALDH1 was expressed in a few cells in most samples except in a few cases of ADH, UDH and normal ducts, in which ALDH1 expression showed extensive distribution. (omicsonline.org)
  • We evaluated the semiquantitative expression of ALDH1 using a score based on positive cells rate and staining density in benign lesions. (omicsonline.org)
  • The score of ALDH1 in luminal epithelial cytoplasm expression is 3, 2, and 1 while myoepithelial cells and stroma expression are 3, 2, and 2 in ADH,UDH and normal ducts respectively. (omicsonline.org)
  • The positive expression of ALDH1 may play a role during the evolution of disease from ADH to breast cancer, as ALDH1 has a predicted value in outcome of breast cancer. (omicsonline.org)
  • A known combination marker of cancer stem cells is high expression of CD44 and low expression of CD24 surface proteins. (weeksmd.com)
  • Scutellaria barbata (barbed skullcap or ban zhi lian) -- This herb contains a polysaccharide called SPS2p that can increase the expression of E-cadherin , a mediator of cell adhesion. (naturalnews.com)
  • Based on these findings, the researchers concluded that YGJDSJ could inhibit TGF- B 1-induced EMT and mediates the metastatic potential of liver cancer cells by down-regulating Smad3 phosphorylation and Snail expression. (naturalnews.com)
  • Moreover, genistein can inhibit the expression of notch-1, p-NF-κB and NF-κB, while promote the expression of Bax/Bcl-2 and caspase-3 in HT-29 cells. (biomedcentral.com)
  • Real-time quantitative polymerase chain reaction and Western blotting were used to detect AQP3 expression in MCF-7 and MDA-MB-231 cells after human hepatocyte growth factor (hHGF) treatment and/or transfection with c-Met silencing small interfering (si)RNA. (scite.ai)
  • Conclusions Our study indicated that AQP3 expression was regulated by c-Met in human breast cancer. (scite.ai)
  • The metastatic cell line SW620 showed high TG2 expression compared to the primary tumour cell lines SW480 and RKO and could form tumour spheroids under non- adherent conditions. (oncotarget.com)
  • TGFβ1 expression in CRC cells, and its level in the cell medium and extracellular matrix was increased in primary tumour CRCs overexpressing TG2 and could regulate TG2 expression and EMT by both canonical (RKO) and non-canonical (RKO and SW480) signalling. (oncotarget.com)
  • The gene expression patterns of the metastatic cancers displayed gene expression profiles that unambiguously identified them as having gone through EMT. (sciencedaily.com)
  • Moreover, it exclusively detects live CTCs, and its detection efficacy is not impacted by the variation of epithelial cell adhesion molecule expression. (uh.edu)
  • Notably CDKL2 is expressed significantly higher in mesenchymal human breast cancer cell lines than in epithelial lines and its over-expression/amplification in human breast cancers is associated with shorter disease-free survival. (healthcarecoremeasures.com)
  • Besides changes in EMT marker expression HMLE cells expressing some kinases such as CDKL2 ZAK FYN and MET lost cell-cell contact Mubritinib and acquired a spindle fibroblast-like mesenchymal morphology (Fig. (healthcarecoremeasures.com)
  • The chronic CNM-exposed cells exhibited CSC-like properties as indicated by 3D spheroid formation, anoikis resistance, and CSC marker expression. (cdc.gov)
  • Cell viability was assessed by Sulforhodamine B assay. (biomedcentral.com)
  • One unit is defined as the dilution giving half maximal release of nitric oxide from C3H/HeJ mouse peritoneal exudate cells in the standard assay. (enzolifesciences.com)
  • To assess the linearity of the assay, samples were spiked with high concentrations of human NODAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. (cusabio.com)
  • The recovery of human NODAL spiked to levels throughout the range of the assay in various matrices was evaluated. (cusabio.com)
  • The EMT process is an essential component of embryonic development and allows for reduced cell adhesiveness and increased cell movement. (sciencedaily.com)
  • In a 2019 study , K3 inhibited the growth of H. pylori (although this research was performed in test tubes, not in humans). (greatist.com)
  • Transforming growth factor β1 exposure of in vitro cultured primary clear cell renal cell carcinoma cells resulted in cells adopting a mesenchymal morphology similar to sarcomatoid clear cell renal cell carcinoma. (lu.se)
  • We therefore suggest that sarcomatoid clear cell renal cell carcinoma morphologically and immunohistochemically may represent a completed epithelial-mesenchymal transition and that transforming growth factor β1 could be an important driving force during the sarcomatoid transdifferentiation of clear cell renal cell carcinoma. (lu.se)
  • Treatment with mAb FAP5-DM1 induced long-lasting inhibition of tumor growth and complete regressions in xenograft models of lung, pancreas, and head and neck cancers with no signs of intolerability. (aacrjournals.org)
  • It is acknowledged that tumor-stromal cell communication is driven by a complex and dynamic network of cytokines, growth factors and proteases. (scite.ai)
  • cancer cells lack the presence of Lefty, thus leading to uncontrolled tumor growth. (cusabio.com)
  • Through their efforts, we are gaining a better understanding of cancer formation and developing diagnostic tools for early detection, while also exploring ways to prevent tumor growth and much more. (lu.se)
  • Cell Death Dis, 2023 Feb 10. (nih.gov)
  • At the end of 2023, their work received a significant boost through generous funding of SEK 77.5 million from the Swedish Research Council, the Swedish Cancer Society and the Swedish Childhood Cancer Fund. (lu.se)
  • Cancer genetics 2023 9 278-279 62-70. (cdc.gov)
  • The researchers found 85 percent of GD2-positive breast cancer cells were CD44 high/CD24 low, while only 1 percent of GD2-negative cells shared that characteristic. (weeksmd.com)
  • This study aims to evaluate the potential anti-metastatic activities of OFE on human ovarian cancer cells, and investigate its inhibitory effect on epithelial-to-mesenchymal transition (EMT). (biomedcentral.com)
  • As controls, 16 samples of normal lung cDNA were also analyzed, each obtained from a pathologically normal section of lung from a cancer patient. (jci.org)
  • Duchesnea indica (mock strawberry or she mei) -- A recent study reported that D. indica extracts could inhibit highly metastatic lung adenocarcinoma cells by down-regulating EMT-related proteins. (naturalnews.com)
  • Lung cancer is a severe form of cancer and detecting it at an early stage is critical for effective treatment. (lu.se)
  • Dr. Sandra Lindstedt, a senior consultant in thoracic surgery at Skåne University Hospital and an adjunct professor at Lund University, is leading a team to develop a non-invasive diagnostic tool for lung cancer. (lu.se)
  • The project funded by Cancerfonden, will focus on using exhaled breath particles as a non-invasive method for diagnosing and monitoring non-small-cell lung cancer. (lu.se)
  • By continuously analyzing breath particles, we anticipate the ability to detect any signs of lung cancer recurrence at an early phase, facilitating prompt medical attention and tailored treatment strategies," explains Sandra Linstedt. (lu.se)
  • Concordance of Abundance for Mutational EGFR and Co-Mutational TP53 with Efficacy of EGFR-TKI Treatment in Metastatic Patients with Non-Small-Cell Lung Cancer. (cdc.gov)
  • It is critical to obtain a complete history, including medication history, drug use, social history, occupational, recreational, and environmental respiratory exposure history, risk factors for human immunodeficiency virus infection, and review of systems, to ensure other causes of interstitial lung disease are excluded. (medscape.com)
  • The activation of this pathway has been documented in all cellular types analyzed up to date, including hepatocytes and HCC cells. (wikipedia.org)
  • We show here that, like SRC-2, SRC-3 is expressed in the epithelial and stromal cellular compartments of the human endometrium during the proliferative and secretory phase of the menstrual cycle as well as in cultured HESCs. (frontiersin.org)
  • These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
  • Acts upstream of or within cellular response to DNA damage stimulus and positive regulation of G1/S transition of mitotic cell cycle. (nih.gov)
  • An important cellular transition also comes into play. (weeksmd.com)
  • Moreover, active retrotransposons cause genomic instability and cellular malfunction in tumor cells, which presents a conundrum, because active retrotransposons are therefore likely to have both good and bad consequences for the placenta. (databasefootball.com)
  • The molecular interaction relationships between TME cells, such as MIF-CD44 and TNFSF9-TNFRSF9, might be an attractive target for developing novel immunotherapeutic agents. (elifesciences.org)
  • Biology of Stem Cells and the Molecular Basis of the Stem State. (iiarjournals.org)
  • We aimed to examine the molecular processes underlying the effects of silibinin in oral cancer in vitro and in vivo as well as its potential anticancer effects. (jcancer.org)
  • Next, we investigated the molecular processes underlying both in vitro and in vivo outcomes of silibinin treatment on oral cancer. (jcancer.org)
  • But on the molecular level, the cells were very different. (sciencedaily.com)
  • Alternatively, identification of molecular mechanism underlying esophageal tumorigenesis could be helpful for the better treatment of esophageal cancer patients. (biomedcentral.com)
  • and (i i) to develop (epi)genomic powerful molecular and/or cell biology and close col aboration to create synergies methodologies, profiling strategies, and functional genomics tools, recent progress and better exploit and further expand bioinformatics tools and resources that in understanding of the cancer (epi) unique research tools and expertise. (who.int)
  • Sarcomatoid conversion of clear cell renal cell carcinoma in relation to epithelial-to-mesenchymal transition. (lu.se)
  • These biphasic neoplasms are called sarcomatoid clear cell renal cell carcinoma. (lu.se)
  • In the present study we investigate whether the sarcomatoid conversion of clear cell renal cell carcinoma could be interpreted as linked to the process of epithelial-mesenchymal transition. (lu.se)
  • Renal cell carcinoma (RCC) incidence has increased over the past two decades. (hindawi.com)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
  • Renal Cell Carcinoma. (iiarjournals.org)
  • Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
  • Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)
  • According to the American Society of Clinical Oncology (ANCO) guidelines on breast cancer follow-up and management, symptoms of breast cancer recurrence include presence of new breast lumps, pain in the bone, chest or abdomen, dyspnea and constant headaches ( 5 ). (iiarjournals.org)
  • According to Benedict Benigno, collaborating physician on the paper, CEO of the Ovarian Cancer Institute and director of gynecological oncology at Atlanta's Northside Hospital, "These results clearly indicate that metastasizing ovarian cancer cells are very different from those comprising the primary tumor and will likely require new types of chemotherapy if we are going to improve the outcome of these patients. (sciencedaily.com)