• Conversion of mesenchyme to epithelium occurs during kidney development, and the embryonic kidney was used to study the role of alpha-dystroglycan for epithelial differentiation. (rupress.org)
  • During in vitro culture of the metanephric mesenchyme, the first morphological signs of epithelial differentiation can be seen on day two. (rupress.org)
  • The formation of the mammalian respiratory unit is initiated during the transition between the pseudoglandular and canalicular/saccular stages and is characterized by coordinated proliferation and differentiation of epithelial and mesenchymal cells. (biomedcentral.com)
  • Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. (sigmaaldrich.com)
  • 1997) whereas laminin isoforms 211 and 221 have a role in smooth muscle cell differentiation. (biolamina.com)
  • Studies of laminin α5-deficient mice indicate that laminins 511 and 521 are essential for normal lobar septation in early lung development and normal alveolarization and distal epithelial cell differentiation and maturation in late lung development (Nguyet et al, 2005). (biolamina.com)
  • Specific laminin isoforms are present in different tissue microenvironments and are essential for cell survival, proliferation, and differentiation. (biolamina.com)
  • Laminin is an extracellular matrix multidomain trimeric glycoprotein and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. (sigmaaldrich.com)
  • 2-5 The basement membrane provides a substrate that promotes epithelial cell adhesion, migration, and differentiation 5-7 and prevents epithelial cell apoptosis. (touchophthalmology.com)
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
  • Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. (sigmaaldrich.com)
  • This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor. (sigmaaldrich.com)
  • Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid. (fmu.ac.jp)
  • Endogenous laminin α5 and bound Lu-Fc were detected with anti-laminin α5 domain LEb/L4b and anti-human IgG, as indicated. (bio-rad-antibodies.com)
  • Laminin is key proteins in the basement membranes of the airway and alveolar epithelium, endothelium, bronchial and vascular smooth muscle cells, nerve cells, and visceral pleura. (biolamina.com)
  • The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits. (lookformedical.com)
  • To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay. (biomedcentral.com)
  • Laminin proteins are integral components of structural scaffolding in animal tissues. (sigmaaldrich.com)
  • Laminin subunit alpha-1 is a protein that in humans is encoded by the LAMA1 gene. (wikipedia.org)
  • ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)
  • Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
  • This study clearly indicates that the epithelial tumor cells synthesize different collagenases and TIMP-1. (uni-bielefeld.de)
  • In UV models of ALCs and human lens epithelial cell lines, the expression levels of histone H3, cell apoptosis factors (Bax/Bcl-2, cleaved caspase-3), and inflammation factors (interleukin-6, tumor necrosis factor-α) were all up-regulated. (techscience.com)
  • Laminin 5 (Ln5) is an extracellular matrix protein that plays an important role in cell migration and tumor invasion. (spandidos-publications.com)
  • Progress in understanding epithelial carcinogenesis has been hindered in the past by a lack of models that faithfully recapitulate the 3-dimensional architecture of tumor-stroma co-evolution. (stanford.edu)
  • Using immunohistochemistry in a well-characterized set of adenocarcinoma tissues, we showed down-regulation of epithelial markers (E-cadherin and cytokeratin 18) and up-regulation of mesenchymal markers (vimentin and α-smooth muscle actin) with concomitant transforming growth factor-β1 (TGF-β1) expression at the invasive margin compared with the central tumor. (aacrjournals.org)
  • Overall, the data show that a double endothelial and epithelial check point controls the transchoroidal migration of neutrophils into the developing brain. (biomedcentral.com)
  • Immunofluorescence showed that dystroglycan is strictly located on the basal side of developing kidney epithelial cells. (rupress.org)
  • Using mouse models, we discovered that mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. (biomedcentral.com)
  • In the developing lung, the specific temporal-spatial interactions between mesenchymal and epithelial cells are carefully orchestrated to permit the gradual establishment of an effective blood-gas barrier (BGB) [ 1 , 2 ]. (biomedcentral.com)
  • Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). (bvsalud.org)
  • Laminin α4 is seen in the pericellular basement membrane of smooth muscle cells lining the airway epithelium (Nguyet and Senior, 2006). (biolamina.com)
  • 2000). Laminin 111, expressed by epithelial and mesenchymal cells in the mouse developing lung, plays a role in branching morphogenesis and in epithelial cell polarity (Schuger et al. (biolamina.com)
  • In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
  • In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)
  • Huh-7 cell line) and mammary epithelial cells (e.g. (dicella.com)
  • The cryopreserved AM contains epithelial cells and is disinfected with antimicrobial agents, attached to nitrocellulose paper with the basement membrane facing upward, stored in a deep freeze at -80°C, and thawed for five to 10 minutes before use. (touchophthalmology.com)
  • It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. (bvsalud.org)
  • We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
  • Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells. (sysy.com)
  • Expanded manifestation of 64 within papillomas undergoing premalignant progression arising from 2-stage chemical carcinogenesis is associated with variations AG-1478 enzyme inhibitor in the connection of cells with laminin in their matrix (5,6). (monossabios.com)
  • Evidence for the importance of sdc-1 in epithelial homeostasis is definitely shown by the fact that sdc-1 manifestation in epithelial cells is definitely controlled via TGF1 mediated cell signaling AG-1478 enzyme inhibitor (16). (monossabios.com)
  • It is critical that we develop a more complete understanding of the dual nature of the cell signaling events controlled by both sdc-1 and TGF1 in epithelial cells. (monossabios.com)
  • The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
  • We profiled DNAm of rectal mucosal biopsies of pediatric UC at diagnosis ( n = 211) and non-IBD control ( n = 85) patients and performed epigenome-wide association studies (EWAS) of specific cell types to understand DNAm changes in epithelial, immune and fibroblast cells across disease states, course, and clinical outcomes. (biomedcentral.com)
  • At diagnosis, UC rectal mucosa exhibited a lower proportion of epithelial cells and fibroblasts, and higher proportion of immune cells, in conjunction with variation in the DNAm pattern. (biomedcentral.com)
  • While treatment had significant effects on the methylation signature of immune cells, its effects on fibroblasts and epithelial cells were attenuated. (biomedcentral.com)
  • The ductal system is coated by a chain of luminal epithelial cells (LECs) situated on a layer of myoepithelial cells (MECs) and encompassed by a distinguished basement membrane. (biomedcentral.com)
  • Two major cell types cooperate to build up the ductal units: inner polarized luminal epithelial cells (LECs) embraced by a layer of myoepithelial cells (MECs), both cell types are rimmed by a cohesive basement membrane (BM) [ 2 ]. (biomedcentral.com)
  • Therefore, damaging the MECs layer resulted in the release of various factors (such as SDF1/CXCL12, CXCL14, MMP, and tenascin) [ 11 , 12 ] with a potential to modify the tumour microenvironment and facilitate the paracrine communication between the tumour epithelial cells and the enclosed stroma enhancing the tumour aggressiveness [ 11 , 13 ]. (biomedcentral.com)
  • found that the most significant changes in laminin and integrin expression pattern were found during the development from the pseudo glandular stage to the canalicular stage (Virtanen et al. (biolamina.com)
  • Lung branching morphogenesis likely involves integrin interactions with laminins. (biolamina.com)
  • The integrin α6 null mouse does not have a reported lung phenotype, however integrin α3/integrin α6 double knockout mice have several features reminiscent of the laminin α5 knockout (De Arcangelis et al. (biolamina.com)
  • Since laminin 111 has been shown to play a role in branching morphogenesis, it has been suggested that laminin 111 could be central for this integrin interaction (Nguyet and Senior, 2006). (biolamina.com)
  • Biological activities of laminin are mediated through binding to a subset of integrin family of cell adhesion receptors, a3b1, a6b1, a6b4, and a7b1 [2]. (mirnamimic.com)
  • Sdc-1 associates with the 3 chain of LM332 via 31 integrin (8) and with the short arm of the laminin 2 chain via 64 integrin (9). (monossabios.com)
  • It is likely that this involves binding of alpha-dystroglycan to E3 fragment of laminin-1. (rupress.org)
  • TissueFab ® - Low endotoxin GelMA-UV Laminin kit enables the precise fabrication of 3D cell models and tissue constructs for research in 3D cell biology, tissue engineering, in vitro tissue models, and regenerative medicine. (sigmaaldrich.com)
  • Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated. (sigmaaldrich.com)
  • The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)
  • 1999). However, branching morphogenesis is not affected in laminin α5, γ2, or α3 knockouts even though integrins α3 and α6 are the primary adhesive receptors for α3 and α5 laminins. (biolamina.com)
  • Laminins are crucial for lung development where different laminin isoforms exhibit specific, non-overlapping functions. (biolamina.com)
  • Moreover, because the putative amino acid sequence of BL0675 shows 30% homology to that of FimA, the major component of type 2 glycoprotein-binding fimbriae of [20, 21], we hypothesized that this five BL0675 variant types may have differing affinities to the carbohydrate chains in mucins. (citiesofdata.org)
  • All five laminin α chains are present during early embryonic lung development but normal adult lung tissue contains primarily laminin α3, α4, and α5 chains (Miner et al,1997). (biolamina.com)
  • Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. (sigmaaldrich.com)
  • The coiled-coil structure of the long arm is formed by three chains (α3, β3 and γ2) covalently linked via interchain disulfide bonds ( 8 ). (spandidos-publications.com)
  • Se localiza en la membrana basal de la lámina lúcida y funciona uniendo las células epiteliales a las membranas basales. (bvsalud.org)
  • The lung is a complex, precisely structured organ in which the vascular network is intimately associated with epithelial-lined tubes and sacs for the prime purpose of gas exchange. (biomedcentral.com)
  • It has become evident that laminin α1 and laminin α5 have central roles in the development of the human lung. (biolamina.com)
  • Laminin α1 expression is restricted to early human lung morphogenesis, whereas the expression of laminin α5 in the human lung is continuous from early lung development through adult life (Pierce et al. (biolamina.com)
  • 1996). Expression of laminin α5 begins in early lung development and persists into adulthood with a widespread expression. (biolamina.com)
  • Laminin 332 is well associated with cutaneous epithelial cell functions, however, laminin γ2 null mice have only minimal changes in lung development, and it's suggested that laminin 332 in the lung have a role in response to injury or disease processes. (biolamina.com)
  • Laminin α2 and α4 null mice survive to adulthood and have no obvious lung abnormalities (Nguyen and Senior, 2006). (biolamina.com)
  • Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)
  • Go¨6976 also induced laminin-dependent cell adhesion in NIH/3T3 and CV-1 fibroblasts, suggesting of a mechanism that may be common to multiple cell-types. (mirnamimic.com)
  • A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)
  • Gel)ma Laminin -UV/365 nm, low endotoxin features a ready-to-use bioink that is formulated for high cell viability, and printability and is designed for extrusion-based 3D bioprinting and subsequent 365 nm light crosslinking and a solution of Laminin to enhance the bioink. (sigmaaldrich.com)
  • PGs, molecules which consist of a protein core that is covalently modified with GAG chains, are distributed both to the ECM "proper" associated with the cell membrane as well as located to intracellular compartment. (hindawi.com)
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • A total of 99 anterior lens capsules (ALCs) of patients with ARC of various nuclear grades, ultraviolet models of ALCs, and two human lens epithelial cell lines (FHL-124 and SRA01/04) were used, and the expression of histone H3, CRYAA, GLB1, and p53 were detected by immunoblotting and reverse transcription and real time-quantitative polymerase chain reaction. (techscience.com)
  • Epithelial ovarian cancer is the major ovarian malignancy and comprises five histological subtypes: serous, mucinous, endometrioid, transitional and clear cell [ 3 , 4 ]. (jcancer.org)
  • The control of this transition from epithelial stem cell to differentiated corneocyte, which is abnormal in epidermal cancers, is not well understood. (stanford.edu)
  • Over the past decades, luminal epithelial cell lineage has gained considerable attraction as the functionally milk-secreting units and as the most fruitful acreage for breast cancer launching. (biomedcentral.com)
  • Functional analyses indicated that some of the genes induced by dragonfly larvae caused an increase in laminins necessary for cell adhesion in the extracellular matrix. (biomedcentral.com)
  • Although the exact mechanism of action is unclear, AM demonstrates physiological properties that promote epithelial and stromal wound healing while suppressing inflammation, fibrosis, and vascularization. (touchophthalmology.com)
  • To address this and to also study the oncogenic potential of unregulated function of dominant regulators of epithelial homeostasis noted above, we developed Multi-Functional Human Tissue Genetics noted above which, when combined with skin tissue regeneration on immune deficient mice, has permitted the molecular reconstruction of events sufficient to trigger human cancer. (stanford.edu)
  • There is increasing evidence that epithelial to mesenchymal transition (EMT) is involved in cancer progression. (aacrjournals.org)
  • Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). (thermofisher.com)
  • Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC were analyzed using immunohistochemistry on tissue microarray containing 205 ESCC and 173 adjacent precursor lesions as well as corresponding normal mucosa. (biomedcentral.com)
  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate mRNA expression, and immunoblotting (IB) was conducted to evaluate protein expression. (jcancer.org)
  • the tadpoles showed reduced expression of cα(E)-catenin , small muscle protein, dystrophin , and myosin light chain genes. (biomedcentral.com)
  • In situ hybridization revealed strong expression of dystroglycan mRNA in all studied epithelial sheets, but not in endothelium or mesenchyme. (rupress.org)
  • Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
  • Non-muscle alpha-dystroglycan is involved in epithelial development. (rupress.org)