Epithelial cells methods cell cycle. Medical search. Frequent questions

Here, we have investigated the role of ChoK in the development of breast cancer and found that ChoK is both necessary and sufficient for growth factor-induced proliferation in primary human mammary epithelial cells and an absolute requirement for the specific mitogenic response to heregulin in breast tumor-derived cells. (aacrjournals.org)
Although the mechanisms mediating the effects of the extracellular matrix (ECM) on breast carcinoma development in vivo are largely unknown, contributing factors may be adhesion mediated signaling and mechanical signals imparted on mammary epithelial cells from surrounding type-I collagen-rich stroma, either directly or across basement membrane proteins. (biomedcentral.com)
To elucidate the role of the p53-repressive effect of ΔNp63α in radiation response, we performed a p63-siRNA knockdown experiment using human mammary epithelial cells (HMECs) expressing ΔNp63α and then performed ectopic and entopic expression experiments using human induced pluripotent stem cells (hiPSCs). (researchsquare.com)

Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. (karger.com)
In LPS-induced ARDS cell model, astragaloside IV up-regulated cell viability, SOD activity and ZO-1 and LC3B I expressions but down-regulated cell apoptosis, TNF-α, IL-6, LC3B II, Beclin-1 and atg5 expressions and LDH and MDA levels. (karger.com)
Astragaloside IV might suppress autophagy initiation directly or indirectly through suppressing the oxidative stress and inflammatory response, which further enhances the cell viability and tight junction and reduces apoptosis in LPS-stimulated pulmonary endothelial ARDS cell model, thus exerting its therapeutic function in ARDS. (karger.com)
RT) qualitative or quantitative PCR and Cheryl A. Nickerson1 thesized virus-encoded protein in host are the primary assays for waterborne *Pacifi c Northwest National Laboratory, cells dying of apoptosis. (cdc.gov)
Cell apoptosis was investigated using 4',6-diamidino-2-phenylindole (DAPI), annexin V, and propidium iodide (PI) fluorescence flow cytometry, which was also used to investigate the cell cycle. (medscimonit.com)
Quercetin also improve the action of the drug 5-Fluorouracil (5-FU) promoting increased expression of p53 and apoptosis in breast cancer T47D cells [ 6 , 7 ]. (biomedcentral.com)
In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)
The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
Using each particle's IC(50) cell viability in a BEAS-2B cell model, pristine nanoclay exposure caused acute loss of monolayer integrity, decreased metabolism, and increased apoptosis. (cdc.gov)
Three different ONCs, however, displayed minimal loss to monolayer integrity despite coating type-dependent differences in apoptosis induction and decreased cell metabolism. (cdc.gov)
The DNA damage response (DDR) is a mechanism that protects cells against radiation-induced oxidative DNA damage by causing cell cycle arrest and apoptosis. (researchsquare.com)
On the other hand, both the ectopic and entopic expression of ΔNp63α in apoptosis-sensitive hiPSCs reduced the expression levels of BAX after irradiation and significantly decreased the number of apoptotic cells induced by radiation. (researchsquare.com)
Ionizing radiation is well known to induce oxidative DNA damage, such as DNA double-strand breaks (DSBs), and consequently trigger the DNA damage response (DDR), including cell cycle arrest and apoptosis. (researchsquare.com)
Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
Recent profiling research suggests that miRNAs are aberrantly expressed in PCa, and these have been implicated in the regulation of apoptosis, cell cycle, epithelial to mesenchymal transition, PCa stem cells, and androgen receptor pathway. (ijcto.org)
Further, we studied the effect of HSP70-2 protein ablation on signaling cascades involved in apoptosis, cell cycle and Epithelial-Mesenchymal-Transition both in culture as well as in-vivo human breast xenograft mouse model. (biomedcentral.com)
Depletion of HSP70-2 in MDA-MB-231 and MCF7 cells resulted in a significant reduction in cellular growth, motility, onset of apoptosis, senescence, cell cycle arrest as well as reduction of tumor growth in the xenograft model. (biomedcentral.com)
In addition, we have examined the putative role of HSP70-2 in apoptosis, cell cycle arrest and epithelial-mesenchymal transition (EMT) in-vitro and in-vivo xenograft mouse model using gene silencing approach. (biomedcentral.com)
Further, cell death effect of ST was associated with induction of apoptosis. (biomedcentral.com)

Viability assays and cell cycle analyses were used to test in vitro sensitivity to different standard and novel treatments. (por-journal.com)
In vitro, the expression of Hes1 was higher in confluent cells maintained under high calcium conditions. (molvis.org)
MLE-12 cells were induced by LPS to construct an ARDS model in vitro. (karger.com)
Indeed, in vitro cell culture acterize the level of virus replication infectivity assays for the waterborne in this system. (cdc.gov)
How- of a highly differentiated intestinal Hong Kong Special Administrative Region, ever, identifi cation of life cycle stages epithelial cell line, the investigators People's Republic of China in cell culture provides evidence of claimed to have established an in vitro infectious oocysts in a water supply. (cdc.gov)
In vitro cell culture tion of high titers of hNoV is valid through microscopy, hybridization of assay for human noroviruses. (cdc.gov)
This study aimed to investigate the effects of xanthoxyletin, a plant-derived coumarin, on human oral squamous cancer cells in vitro and in mouse xenografts in vivo . (medscimonit.com)
Comparative potency of obeticholic acid and natural bile acids on FXR in hepatic and intestinal in vitro cell models. (edu.iq)
Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
The prolyl oligopeptidase inhibitor SUAM-14746 attenuates the proliferation of human breast cancer cell lines in vitro. (cancerindex.org)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
Highthroughput in vitro screening strategies including high content imaging, electric cell impedance sensing, and flow cytometry were employed to evaluate a set of pre- and post-incinerated ONCs for acute effects and fate of the monolayer post-exposure. (cdc.gov)
An integrated high-throughput in vitro screening strategy, using high content imaging and traditional in vitro methods, represents a rapid pulmonary epithelial toxicity assessment approach to prioritize ENMs for further evaluation and serves to inform 'prevention-by-design' material development strategies. (cdc.gov)
These results suggest prominent in vitro anti-proliferative and pro-apoptotic effects of ST in MDA-MB-231 cells. (biomedcentral.com)
In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (nih.gov)
Sääf AM, Halbleib JM, Chen X, Tsan Yuen S, Yi Leung S, Nelson WJ, Brown PO "Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal and Colon Cancer. (openwetware.org)
To gain insight into SARS-CoV biopathology, we analyzed the relevant features of SARS-CoV HSR1 growth in vitro, including the ultrastructural analysis of the consequences of virus replication in Vero cells. (cdc.gov)

Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
This study hypothesized that coating type, incineration status, and time-dependent effects of ONC exposure would impact bronchial epithelial cell monolayer integrity, a key target following inhalation exposure. (cdc.gov)
These results suggest the type of quaternary ammonium coating and incineration status largely impacts mechanism of cytotoxicity, cell metabolism, and the recovery ability of the exposed bronchial epithelial cell monolayer. (cdc.gov)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

Strikingly, histone deacetylase (HDAC) inhibition markedly reduced cell viability by inducing a dose-dependent G0/1 arrest and led to mesenchymal-epithelial transition as evidenced by morphological change and increased E-cadherin and β-catenin expression. (por-journal.com)
Our data suggest that HDAC inhibition is effective in a novel UCS cell line by interfering with both viability and differentiation. (por-journal.com)
LPS effectively inhibited cell viability and LC3B I expression and enhanced LC3B II, Beclin-1 and atg5 expressions in MLE-12 cells. (karger.com)
3-methyladenine promoted cell viability and ZO-1 expression, down-regulated Beclin-1 and atg5 expression, while Rapamycin (Rap) had an opposite effect. (karger.com)
Astragaloside IV suppressed cell viability and ZO-1 expression after the Rap treatment. (karger.com)
The MTT assay assessed cell viability, and autophagy was detected by electron microscopy. (medscimonit.com)
A) Cell viability at 72 hours and (B) 6 days post-siCKAP5 treatment as measured by methoxynitrosulfophenyl-tetrazolium carboxanilide (XTT). (medicalxpress.com)
Values represent average % cell viability compared to cells treated with an equivalent concentration of siControl LNP. (medicalxpress.com)
Analyses of the cell viability, cell cycle, migration, and matrigel invasion assays were performed to elucidate how INT 747 and /or FMD functions in prostate cancer. (edu.iq)
In the present study, cell viability was analyzed using Cell Counting Kit‑8 assay, while inflammation was detected using ELISA and reverse transcription‑quantitative PCR. (spandidos-publications.com)
Thus, the present study aimed to explore the functional roles of hyperforin on IL-13-stimulated nasal epithelial cells and discuss the mechanism by which hyperforin influences nasal epithelial cell viability, inflammation and barrier damage. (spandidos-publications.com)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
This was performed by measuring cell growth, viability, clonogenic activity, cell cycle and reactive oxygen species (ROS) generation. (utmb.edu)
The treatments of CE and COC resulted in the reduction of the growth fraction of alveolar epithelial type II cells without significant decrease in viability. (utmb.edu)

A novel UCS cell line (PF338) was established from the malignant pleural effusion of a 59-year-old patient at time of disease progression. (por-journal.com)
Importantly, UCS is the prototype tumor for epithelial-mesenchymal transition (EMT), a reversible biological process that associates with tumor progression and metastasis and in which epithelial cells transform into more invasive mesenchymal cells by losing their epithelial properties [ 10 - 12 ]. (por-journal.com)
These results demonstrate that ChoK plays an essential role in both normal human mammary epithelial cell proliferation and breast tumor progression. (aacrjournals.org)
In recent years, epithelial-mesenchymal transition (EMT) has been suggested to play a key role in the process of embryonic development, differentiation of tissues and organs, chronic inflammation and fibrosis, as well as cancer progression ( 14 ). (spandidos-publications.com)
EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
As little is known about epithelial-stromal interactions in vivo, it is necessary to visualize the stroma surrounding normal epithelium and mammary tumors in intact tissues to better understand how matrix organization, density, and composition affect tumor formation and progression. (biomedcentral.com)
One important step to elucidating these signaling interactions is to determine the organization of the collagenous stroma surrounding both normal mammary glands and tumors within intact tissue so as to better understand the cell-matrix interaction and how matrix organization, density, and composition affect tumor formation and progression. (biomedcentral.com)
[ 14 ] Subsequent work in our laboratory demonstrated that K17 serves as a nuclear shuttle of p27KIP1, resulting in sustained cell cycle progression. (medscape.com)

In summary, the present results revealed that hyperforin suppressed IL‑13‑induced nasal epithelial cell inflammation and barrier damage by targeting BCL6/p38 MAPK/CCL11, which may provide promising therapeutic targets for AR. (spandidos-publications.com)
However, whether hyperforin is involved in nasal epithelial cell damage caused by AR is unknown. (spandidos-publications.com)

This morphology can be attributed to epithelial-mesenchymal transition (EMT) that often associates with tumor invasion and metastasis. (por-journal.com)
Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Furthermore, proteins involved in epithelial-mesenchymal transition (EMT) were strongly altered, which plays a central role in metastasis. (spandidos-publications.com)
Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
Moreover, miR-141 and miR-488 post-transcriptionally regulated androgen receptor (AR) expression, and inhibited the growth and metastasis of prostate gland epithelial cells. (ijcto.org)

Such continuously renewing epithelia are by definition governed by stem cells. (molvis.org)
Upon division, the slow cycling limbal epithelial stem cells give rise to daughter cells known as transient amplifying (TA) cells. (molvis.org)
It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)
Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
TP63 is a member of the tumour suppressor TP53 gene family, and ΔNp63α, a TP63 splicing variant, is constitutively expressed in the stem cell-containing basal layer of stratified epithelial tissues, including the mammary gland, where it plays a critical role in stemness and tissue development. (researchsquare.com)
This p53-repressive activity may cause genomic instability in epithelial stem cells exposed to radiation. (researchsquare.com)
Taken together, these results indicate that ΔNp63α represses p53-related radiation-induced DDR, thereby potentially causing genomic instability in epithelial stem cells. (researchsquare.com)
Although normally dormant, hair follicle stem cells (HFSCs) quickly become activated to divide during a new hair cycle. (nature.com)
Fuchs, E. The tortoise and the hair: slow-cycling cells in the stem cell race. (nature.com)
Stem Cells 29 , 1459-1468 (2011). (nature.com)
Defining the epithelial stem cell niche in skin. (nature.com)
Beijing Tiantan Puhua Hospital in China provides the world's most advanced stem cell procedures and treatments in Asia's top neurosurgical hospital, bringing new hope and a better quality of life to people all over the globe. (mediescapes.com)
Our senior staff of stem cells procedures is lead by Dr. Wu Li Ke and Dr. Wang Xiao Juan both have years of extensive stem cell research experience conducting clinical trials in China - Hebei Medical University. (mediescapes.com)
Their stem cell experience has focused on the healing of brain trauma, spinal cord injury, cerebral vascular diseases, multiple sclerosis and myelitis, among other illnesses. (mediescapes.com)
In charged of all stem cells operations is Dr. Han Xiao Di, who spent two years at Australia's Alfred Hospital in the neurosurgical department and in the Australia National Trauma Institute. (mediescapes.com)
When it comes to the latest in stem cell healthcare, Tiantan Puhua Hospital doctors have developed a number of unprecedented and world leading stem cell treatment programs including 'Self stem cells Activation and Proliferation Program', neural stem cells implantation by Sterotacxios technique and Spinal Cord Stem Cell Injections. (mediescapes.com)
Systematical profiling and comprehensive annotation of embryonic stem cells lincRNAs may not only bring a clearer big picture of these novel regulators but also shed light on their functionalities. (biomedcentral.com)
Based on multiple RNA-Seq datasets, we systematically identified 300 human embryonic stem cell lincRNAs (hES lincRNAs). (biomedcentral.com)
As the first global profiling and annotating of human embryonic stem cell lincRNAs, this work aims to provide a valuable resource for both experimental biologists and bioinformaticians. (biomedcentral.com)
Combining a comprehensive collection of human embryonic stem cell RNA-Seq datasets with Human BodyMap 2, we validated that 295 previously annotated lincRNAs were expressed in multiple human embryonic stem cell samples and further identified five novel hES lincRNAs through de novo assembling. (biomedcentral.com)
Moreover, we surveyed and manually screened published hES RNA-Seq datasets in several public repositories, resulting in a list of 31 wild-type human embryonic stem cell samples. (biomedcentral.com)

The inverse correlation between Notch signaling and the proliferative status of the corneal epithelium is consistent with the idea that Notch plays a role in corneal epithelial differentiation. (molvis.org)
The molecular functions of upregulated genes in MZ-3 were mainly enriched for transcriptional activity, cell proliferation and cell differentiation. (biomedcentral.com)
The mitochondrial electron transport chain is dispensable for proliferation and differentiation of epidermal progenitor cells. (nature.com)
While more and more long intergenic non-coding RNAs (lincRNAs) were identified to take important roles in both maintaining pluripotency and regulating differentiation, how these lincRNAs may define and drive cell fate decisions on a global scale are still mostly elusive. (biomedcentral.com)
Soen Y, Mori A, Palmer TD, Brown PO "Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. (openwetware.org)

We investigated the role of CAL-101 (GS-1101, Idelalisib) TGF-1 in inducing profibrotic signaling from epithelial cells to activate pericyte-myofibroblast transition. (holyexperiment.org)
Proliferation, survival, and epithelial-mesenchymal transition (EMT) are three fundamental events that are deeply linked to carcinogenesis. (edu.iq)
Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. (edu.iq)
Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)

Moreover, our study showed that INT 747 either alone or combined with FMD robustly induced cell cycle arrest at the S phase. (edu.iq)
JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
While exploring the molecular changes associated with strong ST efficacy in breast cancer cells, we observed that ST induced cell cycle arrest as well as cell death. (biomedcentral.com)
However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (nih.gov)

After irradiation, the expression of DDR-related genes and proteins in ΔNp63α-expressing and control cells was analysed by RT-qPCR, Western blotting, and flow cytometry. (researchsquare.com)
HSP70-2 expression was examined in 154 tumor and 103 adjacent non-cancerous tissue (ANCT) specimens and breast cancer cell lines (MCF7, BT-474, SK-BR-3 and MDA-MB-231) by RT-PCR, quantitative-PCR, immunohistochemistry, Western blotting, flow cytometry and indirect immunofluorescence. (biomedcentral.com)

Dose-dependent response of CKAP5 knockdown across various cancer cell lines. (medicalxpress.com)
Cells that did not show any effect of CKAP5 knockdown are highlighted with red boxes. (medicalxpress.com)

This study was aimed at comparing the toxic effects of eqimolar concentration (1 mM) of COC and CE on alveolar epithelial type II cells. (utmb.edu)
In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (nih.gov)

We hypothesized that FXR and FMD may inhibit proliferation and the metastatic phenotype in PC-3 prostate cancer cells. (edu.iq)
At the cellular level, FXR activation resulted in down-regulation of procaspase -3, vimentin, and MMP9, which triggers apoptotic cell death, cell cycle arrest, and switch from mesenchymal to an epithelial phenotype. (edu.iq)
During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
Methods: To reveal the phenotype-associated cell type in the development of vulnerable plaque and to identify hub gene for pathological process, we combined single-cell RNA and bulk RNA sequencing datasets of human atherosclerotic plaques using Single-Cell Identification of Subpopulations with Bulk Sample Phenotype Correlation (Scissor) and Weighted gene co-expression network analysis (WGCNA). (bvsalud.org)
The aim of our study was to determine the molecular phenotype of NEC via transcriptomic and epithelial cell-specific epigenomic analysis, with a specific focus on DNA methylation. (biomedcentral.com)

In UCS, TP53 has been identified as the most frequently mutated gene, followed by mutations in the PI3K pathway, KRAS , cell cycle regulators including FBXW7 and chromatin remodeling and histone genes including ARID1A [ 7 - 9 ]. (por-journal.com)
Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. (nature.com)
DUSP4 alterations lead to hyperactivation of MAPK signaling in many cancers, including breast cancer, which often harbor mutations in cell cycle checkpoint genes, particularly in TP53. (biomedcentral.com)
Interestingly, replication stress in Brca2-null cells activates p53 and the expression of its target genes, including senescence-inducing Ink4/Arf. (biomedcentral.com)
Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
Transcriptomic analysis showed decreased RNA expression of cell cycle-related genes and increased expression of programmed cell death-related genes in sip63 cells compared to scr cells. (researchsquare.com)
Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
Function annotation of target genes indicated that most of them tend to target genes involved in signal transduction and cell communication, especially cancer-related PI3K-Akt and p53 signaling pathway. (ijcto.org)
Redundant gene models were identified and merged based on the genomic coordinates, resulting in 5,571 standalone annotated lincRNA genes (See Methods and Materials, as well as the Additional File 1 for more details). (biomedcentral.com)
We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (nih.gov)

Specifically, we identified DNA methylation differences in intestinal epithelial cell genomic DNA within resected ileum or colon that may provide new insights into the biology and mechanisms of NEC pathogenesis. (biomedcentral.com)
We prepared a total of n = 40 bisulfite sequencing libraries from individual tissue samples following laser capture microdissection of intestinal epithelial cells in each case. (biomedcentral.com)
Once ingested, sporozoites are released from the oocysts into intestinal epithelial cells. (biomedcentral.com)
Overview of Intestinal Protozoan and Microsporidia Infections Protozoa is a loose term for certain nucleated, unicellular organisms (eukaryotes) that lack a cell wall and are neither animals, plants, nor fungi. (msdmanuals.com)

The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
Nature Reviews Molecular Cell Biology. (edu.iq)
These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
At molecular level, down-regulation of HSP70-2 resulted in reduced expression of cyclins, cyclin dependent kinases, anti-apoptotic molecules and mesenchymal markers and enhanced expression of CDK inhibitors, caspases, pro-apoptotic molecules and epithelial markers. (biomedcentral.com)
This study provides valuable insight into the chemopreventive efficacy and associated molecular alterations of ST in breast cancer cells whereas it had only moderate efficacy on lung cancer cells and did not show any considerable effect on skin cancer cells. (biomedcentral.com)
American Journal of Respiratory Cell and Molecular Biology. (lu.se)

Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
Our data showed that diacetyl and 2,3-pentanedione induced significant transcriptomic changes, including those related to ciliogenesis, in the primary NHBE cells. (nature.com)
We previously reported host cell transcriptomic (mRNA) responses to an ocular isolate of Ad5 (see IOVS 46, 3657-3665, 2005), and now explore the responses to Ad19. (arvojournals.org)

We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
Consistent with this observation, primary tumor explants cultured in a randomly organized collagen matrix realigned the collagen fibers, allowing individual tumor cells to migrate out along radially aligned fibers. (biomedcentral.com)

Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Among these features are the peculiar tropism of the virus for Vero cells (a continuous cell line established from monkey kidney epithelial cells), its capacity for growth at 37°C (while other respiratory coronaviruses grow at lower temperatures), and its ability to infect lower respiratory tract tissues ( 13 ). (cdc.gov)

The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)

Whereas immunohistochemistry of the metastatic tumor showed a predominantly sarcomatous vimentin positive tumor that has lost E-cadherin expression, PF338 cells demonstrated biphasic growth and carried mutations in KRAS , PIK3CA , PTEN and ARID1A . (por-journal.com)
Collectively, FXR activation alone markedly decreases, and when combined with FMD abrogates the survival and carcinogenic potential of metastatic prostate cancer cells. (edu.iq)

Renal cysts were recognized by the presence of a cuboidal epithelial lining and those with a diameter above 50?m were scored while positive. (immune-source.com)
K17 was not detected in the epithelial cells of benign glandular lesions, but groups of cuboidal cells, residing beneath the epithelial layer of benign glands, were frequently positive for K17, especially in cases of microglandular hyperplasia. (medscape.com)

PF338 tumor cells were resistant to MEK- and TGF-β signaling-inhibition but sensitive to PIK3CA- and PARP-inhibition and first-line chemotherapeutics. (por-journal.com)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
Yu J, Yang K, Zheng J, Zhao W, Sun X. Synergistic tumor inhibition of colon cancer cells by nitazoxanide and obeticholic acid, a farnesoid X receptor ligand. (edu.iq)
In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)

After Cryptosporidium oocysts are ingested, they excyst in the gastrointestinal tract and release sporozoites, which parasitize gastrointestinal epithelial cells. (msdmanuals.com)
When the infective oocysts are ingested by humans or another vertebrate host, the cycle begins again. (msdmanuals.com)

In addition, epithelial cell cycle G2/M arrest and production of profibrotic cytokines were both attenuated. (holyexperiment.org)
Th2 cytokines also induce the breakdown of the epithelial barrier in allergic diseases, and epithelial cells release cytokines to promote Th2 immune response ( 6 ). (spandidos-publications.com)
Many studies have reported their regulatory roles in immunity through cytokines and cell signaling. (cancerindex.org)

Moreover, hyperforin promotes the expression of tight junction proteins in endothelial cells after blood-brain barrier injury ( 14 ). (spandidos-publications.com)

While the mechanisms of tumorigenesis in FH-deficient cells have been extensively looked into, the metabolic changes caused by the loss of FH activity have only been partially tackled. (immune-source.com)
Toxicology Mechanisms and Methods , 25 (8), 604-613. (utmb.edu)
Klapholz-Brown Z, Walmsley GG, Nusse YM, Nusse R, Brown PO "Transcriptional program induced by wnt protein in human fibroblasts suggests mechanisms for cell cooperativity in defining tissue microenvironments. (openwetware.org)

Pathogenesis of a tive PCR to assess the level of viral fl uorescent in situ hybridization the genogroup II human norovirus in gnotobi- production in cell culture, where we presence of viral RNA through 5 pas- otic pigs. (cdc.gov)
The study included SCC-1 human oral cancer cells and EBTr normal embryonic bovine tracheal epithelial cells, which were treated with 0 µM, 5 µM, 10 µM, and 20 µM of xanthoxyletin for 24 hours. (medscimonit.com)
Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
Human conjunctival epithelial cells in primary culture were infected with Ad19 at multiplicities of infection (MOI) of 0, 0.1 or 1.0 and harvested at 1.5h, 6h or 16h post-infection. (arvojournals.org)
We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
We show here that, like SRC-2, SRC-3 is expressed in the epithelial and stromal cellular compartments of the human endometrium during the proliferative and secretory phase of the menstrual cycle as well as in cultured HESCs. (frontiersin.org)
Here, we evaluated the efficacy of ST on three different human cancer cell lines including skin epidermoid carcinoma A431 cells, lung epithelial carcinoma A549 cells and breast adenocarcinoma MDA-MB-231. (biomedcentral.com)
Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)
Of which, one forth (78 out of 300) hES lincRNAs were further identified to be biasedly expressed in human ES cells. (biomedcentral.com)
Palmer C, Diehn M, Alizadeh AA, Brown PO "Cell-type specific gene expression profiles of leukocytes in human peripheral blood. (openwetware.org)
As these kinds of health effects data become available and methods to assess levels of significant human exposure improve, these MRLs will be revised. (cdc.gov)

The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)

In the most well characterized "canonical" pathway in mammalian cells, the Notch intracellular domain (NotchIC) translocates to the nucleus where it mostly associates with the recombination signal binding protein for the immunoglobulin kappa J region (RBPJκ). (molvis.org)
The expression levels of B‑cell lymphoma 6 (BCL6) and the p38 MAPK/C‑C motif chemokine 11 (CCL11) pathway were detected using western blotting. (spandidos-publications.com)
These findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. (biomedcentral.com)
In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
However, dysregulation of the DDR pathway allows cells to proliferate beyond senescence limits. (oncotarget.com)
Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

Using laser capture microdissection, epithelial cell-specific methylation signatures were characterized by whole-genome bisulfite sequencing of ileal and colonic samples at the time of surgery for NEC and after NEC had healed at reanastomosis ( n = 40). (biomedcentral.com)
Blood cell DNA methylation biomarkers in preclinical malignant pleural mesothelioma: The EPIC prospective cohort. (who.int)

The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
1980), lates cell proliferation in the rat respiratory tract (Roemer et al. (cdc.gov)

We found that Dusp4 loss alone is insufficient in mediating tumorigenesis, but alternatively converges with loss in Trp53 and MYC amplification to induce tumorigenesis primarily through chromosome 5 amplification, which specifically upregulates Dbf4 , a cell cycle gene that promotes cellular replication by mediating cell cycle checkpoint escape. (biomedcentral.com)
This study identifies a novel mechanism for breast tumorigenesis implicating Dusp4 loss and p53 mutations in cellular acquisition of Dbf4 upregulation as a driver of cellular replication and cell cycle checkpoint escape. (biomedcentral.com)
Stromal-epithelial interactions are of particular significance in breast tissue as misregulation of these interactions can promote tumorigenesis and invasion. (biomedcentral.com)

Notch signaling is initiated by the interaction between the ligand on one cell and the receptor on a neighboring cell. (molvis.org)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)

Mitochondrial function in murine skin epithelium is crucial for hair follicle morphogenesis and epithelial-mesenchymal interactions. (nature.com)

Disruption of bronchial cell monolayer integrity by organomodified nanoclays and their incinerated byproducts. (cdc.gov)
Conversely, incinerated nanoclay byproducts caused decreased monolayer integrity, increased cell necrosis, and little evidence for reestablishment of the epithelial monolayer. (cdc.gov)

The results revealed that hyperforin inhibited IL‑13‑induced nasal epithelial inflammatory cytokine release and repressed the damage to the cellular barrier from IL‑13 stimulation. (spandidos-publications.com)
We also report that HSP70-2 plays an important role in cellular growth, migration and invasion of breast cancer cells and tumor growth of breast cancer xenograft. (biomedcentral.com)

For the intracellular metabolomic analysis, cells were quickly washed three instances with PBS to remove contaminations from the metabolites in the press. (immune-source.com)
Cryptosporidia are obligate, intracellular coccidian protozoa that replicate in small-bowel epithelial cells of a vertebrate host. (msdmanuals.com)

LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
The DNA guardian protein p53 plays the most important role in DDR: it promotes DNA repair and the elimination of cells that are unable to repair the damage caused by oxidative stresses, including radiation. (researchsquare.com)
We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

Compared with other ENMs, little information exists on risks to occupational pulmonary health along the ONC life cycle that encompass synthesis, handling, manipulation, and disposal. (cdc.gov)

Epithelial-stromal interactions in normal mammary glands, mammary tumors, and tumor explants in three-dimensional culture were studied with histology, electron microscopy, and nonlinear optical imaging methodologies. (biomedcentral.com)
In particular, local cell invasion was found predominantly to be oriented along certain aligned collagen fibers, suggesting that radial alignment of collagen fibers relative to tumors facilitates invasion. (biomedcentral.com)
The presentation of these tumor-associated collagen signatures allowed us to identify pre-palpable tumors and see cells at the tumor-stromal boundary invading into the stroma along radially aligned collagen fibers. (biomedcentral.com)
Macrophages are essential inflammatory cells which regulate the features of immune reactions within tumors. (cancerindex.org)

Cell Culture Assay Straub et al. (cdc.gov)
Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)

The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
[ 15 ] Other studies have shown K17 to be a negative prognostic indicator in gastric adenocarcinoma, bladder carcinoma, epithelial ovarian carcinoma, and triple-negative breast carcinoma. (medscape.com)

However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)
Background Loss of function of fumarate hydratase (FH), the mitochondrial tumor suppressor and tricarboxylic acid (TCA) cycle enzyme, is associated with a highly malignant form of papillary and collecting duct renal cell malignancy. (immune-source.com)
In adenocarcinomas, K17 staining was detected in a mean of 33.9% of malignant cells. (medscape.com)

Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)

Interestingly, the combination treatment on PC-3 cells not only showed several lines of evidence of apoptotic cells death but also inhibited carcinogenic potential as evaluated by impairment of spheroid formation capacity and delayed wound healing and matrigel invasion. (edu.iq)

Results: Macrophages were found to be strongly correlated with plaque vulnerability while vascular smooth muscle cell (VSMC), fibrochondrocyte (FC) and intermediate cell state (ICS) clusters were negatively associated with unstable plaque. (bvsalud.org)
The degradability of the isolates to cellulose was then determined by the DNS colorimetric method, and the results showed that Bacillus had the highest degradation rate. (bvsalud.org)
In the natural image domain, spatial embedding-based instance segmentation methods are known to yield high-quality results, but their utility to biomedical data is largely unexplored. (mpi-cbg.de)

Materials and Methods Coll-GFP Mice Coll-GFP transgenic mice were generated around the C57BL6 background as described previously.2 In brief, 3.2 kb of CAL-101 (GS-1101, Idelalisib) the collagen I(1) (Col1a1) promoter and enhancer with the open reading frame of enhanced GFP yielded the highest levels of GFP expression when gene transcripts were generated. (holyexperiment.org)

Cervical cancer has multiple histologic subtypes, with squamous cell carcinoma (SCC) accounting for approximately 75% of cases and invasive adenocarcinoma accounting for 10% to 15% of cases. (medscape.com)

Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)
Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). (medscape.com)
[ 11-13 ] By contrast, we discovered, using laser capture microdissection and mass spectrometry, that keratin 17 (K17) could predict the overall survival of patients with cervical squamous cell carcinoma more accurately than grade, stage, or any other clinicopathologic features. (medscape.com)

In UCS, several studies identified characteristic EMT-related expression signatures including active TGF-β signaling in tissue and cell lines [ 7 , 11 , 13 , 14 ]. (por-journal.com)
Telomere maintenance is normally controlled by telomerase activity as well as telomerase-associated factors throughout the cell cycle in tissue- and cell type-specific manners [ 1 - 3 ]. (oncotarget.com)

To find miRNAs differentially expressed in PCa and their relation to prognostic factors and therapeutic potentials, we studied 24 surgical specimens from men who underwent radical prostatectomy, through high-throughput Illumina sequencing and quantitative real-time PCR (qRT-PCR) methods. (ijcto.org)

Using a genetically engineered mouse model, we generated mammary-specific Dusp4 -deleted primary epithelial cells to investigate the necessary conditions in which DUSP4 loss may drive breast cancer oncogenesis. (biomedcentral.com)
To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)

This CAC is associated with the cell cycle and its regulation is affected by the tumor suppressor proteins. (biomedcentral.com)
Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
As an important family of transcription factors, E2F family transcription factors (E2Fs) play vital roles in cell proliferation and regulation. (bvsalud.org)

Given the previously reported role of DUSP4 as a p53 target, a potential cell cycle checkpoint, and in mediating senescence downstream of replication stress, we hypothesized that DUSP4 may be a critical oncogenic driver in breast cancer. (biomedcentral.com)
Because tumor-derived, cell-free DNA (cfDNA) is often released from cancer cells and circulates in the bloodstream, we hypothesized that breast cancer development is associated with changes in the amount of telomeric cfDNA that can be detected in the plasma. (oncotarget.com)
HSP70-2 expression was also observed in all breast cancer cells (BT-474, MCF7, MDA-MB-231 and SK-BR-3) used in this study. (biomedcentral.com)

By using Fh1-deficient mouse epithelial kidney cells we have recently demonstrated that in the absence of Fh1, the TCA cycle is definitely truncated causing an build up of fumarate and succinate paralleled by a decrease of malate and citrate. (immune-source.com)

It is a highly conserved network that orchestrates cell-fate decisions in the nervous, gastrointestinal, and the hematopoietic systems of many organisms ranging from insects to humans. (molvis.org)

For instance, hyperforin can improve imiquimod-induced psoriasis-like skin inflammation in mice by regulating γδ T cells that produce IL-17A ( 13 ). (spandidos-publications.com)

Anatomy52

Organisms14

Diseases5

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes71

Disciplines and Occupations1

Information Science3

Mammary epithelial cells3

Apoptosis22

Vitro20

Bronchial epithelial5

Viability15

Progression9

Nasal epithelial cell2

Metastasis5

Stem22

Differentiation5

Transition4

Induced cell cycle arrest4

Flow cytometry2

Knockdown2

Alveolar2

Phenotype5

Genes10

Intestinal4

Molecular6

Transcriptomic3

Migrate2

Tissues6

Extracellular matrix2

Metastatic2

Cuboidal2

Inhibition4

Oocysts2

Cytokines3

Endothelial cells1

Mechanisms3

Human13

Assays1

Pathway7

Methylation2

Respiratory tract2

Tumorigenesis3

Receptor3

Morphogenesis1

Monolayer2

Cellular2

Intracellular2

Protein5

Pulmonary1

Tumors4

Assay2

Ovarian2

Malignant3

Collectively1

Invasion1

Results3

Gene1

Adenocarcinoma1

Squamous-cell carc3

Tissue2

Differentially expressed1

Investigate2

Regulation4

Breast cancer3

Kidney1

Gastrointestinal1

Inflammation1