• Tunel assay of cells exposed to 2.4 microg/cm2 MW for 24 hours showed no evidence of apoptosis. (cdc.gov)
  • To pursue the goals of this project, isogenic cell lines along with techniques to assess growth control, apoptosis and DNA repair will be employed. (europa.eu)
  • Analysis of chemically distinct conjugates, resistance models, and biomarkers implicates a unique mode of action, with mitotic arrest and apoptosis of malignant epithelial cells coupled to disruption of fibroblastic and vascular structures. (aacrjournals.org)
  • This study was performed to determine the effects of human placenta mesenchymal stem cell (hPMSC) transplantation on granulosa cell apoptosis and anti-Müllerian hormone (AMH) and follicle-stimulating hormone receptor (FSHR) expression in autoimmune drug-induced premature ovarian failure (POF) mice. (biomedcentral.com)
  • Granulosa cell apoptosis of the ovaries was examined by In Situ Cell Death Detection Kit. (biomedcentral.com)
  • Granulosa cells were isolated and treated with SiAmh interference and hPMSC supernatant to observe the effects of AMH expression on granulosa cell apoptosis in vitro. (biomedcentral.com)
  • granulosa cell apoptosis was decreased following hPMSC transplantation. (biomedcentral.com)
  • hPMSC transplantation can significantly improve the serum levels of high gonadotropin and low estrogen of POF mice, promote follicular development, inhibit excessive follicular atresia and granulosa cell apoptosis, and improve the ovarian reserve capacity. (biomedcentral.com)
  • Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • When human kidney tubular cells (HK-2) were stimulated with TGF-ß1, they produced and released increased amounts of exosomes (TGFß-Exo), which prevented renal interstitial fibroblasts from apoptosis. (bvsalud.org)
  • Knockdown of TNFAIP8 in tubular cells abolished the ability of TGFß-Exo to prevent fibroblast apoptosis. (bvsalud.org)
  • Quercetin also improve the action of the drug 5-Fluorouracil (5-FU) promoting increased expression of p53 and apoptosis in breast cancer T47D cells [ 6 , 7 ]. (biomedcentral.com)
  • Despite an intact canonical pathway BIIE 0246 we did not observe any significant increase in cell cycle arrest or apoptosis upon TGFβ treatment of Py2T cells (data not shown). (researchtoactionforum.org)
  • First, in the T. spiralis infection model, H&E staining was used to analyse the damage to jejunum tissue, a TUNEL assay was used to examine cell apoptosis, and the expression of ERS-related and apoptosis-related molecules was also measured. (biomedcentral.com)
  • The results showed that TsKaSPI could induce apoptosis in a porcine small intestinal epithelial cell line (IPEC cells) by activating ERS and promote activation of the NF-κB signalling pathway. (biomedcentral.com)
  • The results confirmed that TsKaSPI could activate ERS and lead to apoptosis in intestinal epithelial cells. (biomedcentral.com)
  • In conclusion, T. spiralis infection and TsKaSPI can promote cell apoptosis by activating the ERS response in intestinal epithelial cells and activate the NF-κB signalling pathway to promote the occurrence and development of inflammation. (biomedcentral.com)
  • Therefore, we hypothesized that T. spiralis SPI (TsSPI) may be involved in intestinal epithelial cell apoptosis induced by T. spiralis infection, but the specific mechanism remains to be further explored. (biomedcentral.com)
  • The researchers 12 used multiple tests to determine cell viability and apoptosis after exposure to ivermectin. (freelysocial.com)
  • Cultured cells were also exposed to concentrations of ivermectin after which cell viability and apoptosis were measured. (freelysocial.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • Non-motile primary cilia are solitary sensor organelles playing a critical role in cell cycle control, proliferation, polarity and differentiation, particularly of ciliated cells possessing motile cilia [ 3 , 4 ]. (ersjournals.com)
  • Primary cilia may be crucial in determining outcomes during airway epithelial cell differentiation thus we hypothesised that primary cilia are present in adult epithelial cells and may play a key role in airway plasticity. (ersjournals.com)
  • Our data suggest that HDAC inhibition is effective in a novel UCS cell line by interfering with both viability and differentiation. (por-journal.com)
  • The models use isolated primary support cells cultured onto a biological scaffold and within a microfluidic system to create a niche and support the desired differentiation of epithelia, germ and somatic cells from patient-derived induced pluripotent stem cells. (biomedcentral.com)
  • whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
  • This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
  • Although pHi was previously thought to be relatively constant as a homeostatic mechanism, we now know that pHi changes during normal cell cycle progression, cell migration, and cell differentiation. (ucsf.edu)
  • We also found that increased pHi is necessary for adult and embryonic stem cell differentiation as well as lineage specification (Ulmschneider et al. (ucsf.edu)
  • In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
  • 2 The authors applied their DC3 method in the joint analysis of scRNA-seq, single-cell ATAC sequencing (scATAC-seq) and bulk chromatin immunoprecipitation (Hi-ChIP) on mouse embryonic stem cells after four days of retinoic acid-induced differentiation. (technologynetworks.com)
  • Epithelial differentiation was confirmed by positive staining for E-cadherin and pan-cytokeratin. (biomedcentral.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • that regulate the differentiation and activation of these cells. (cdc.gov)
  • Taken together, the analyses enabled researchers to define the biological pathways that are relevant to injury time-course and niches, including the signatures underlying epithelial repair that predicted maladaptive states associated with a decline in kidney function. (hospitalhealthcare.com)
  • A major aspect our research program is determining in molecular detail how intracellular (cytoplasmic) pH (pHi) dynamics regulates cell behaviors, with a focus on epithelial plasticity. (ucsf.edu)
  • Thus, targeting fibroblast cell activation and pEMT are critical strategies for attenuating fibrosis. (nature.com)
  • In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • Co-culture of human being main epithelial cells with irradiated 3T3 fibroblast feeder cells (J2 cells) and the Rho kinase inhibitor Y-27632 (Y) allows for the unrestricted growth of cells of epithelial source by the process termed conditional reprogramming. (cell-metabolism.com)
  • When keratinocytes Rabbit Polyclonal to CSTL1 are co-cultured with irradiated 3T3 fibroblast feeder cells (J2 cells) and growth factors [1, 4, 5], they undergo continuous replication over many decades resulting in their immortalization. (cell-metabolism.com)
  • Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
  • This morphology can be attributed to epithelial-mesenchymal transition (EMT) that often associates with tumor invasion and metastasis. (por-journal.com)
  • Importantly, UCS is the prototype tumor for epithelial-mesenchymal transition (EMT), a reversible biological process that associates with tumor progression and metastasis and in which epithelial cells transform into more invasive mesenchymal cells by losing their epithelial properties [ 10 - 12 ]. (por-journal.com)
  • Tumor cell invasion and metastasis require cancer cells to be both proliferative and invasive, i.e. migrate through the tissue and assemble invadopodia. (biorxiv.org)
  • Metastasis is a complex, multi-step process that is initiated when cancer cells in the primary tumor acquire invasive properties, including motility and the ability to breakdown the extracellular matrix (ECM) 1 , and is responsible for the majority of cancer-related mortalities 2 . (biorxiv.org)
  • Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
  • A novel UCS cell line (PF338) was established from the malignant pleural effusion of a 59-year-old patient at time of disease progression. (por-journal.com)
  • Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. (nature.com)
  • MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. (nature.com)
  • In the present study, the effect of miR-183 on cell viability, cell cycle progression, and migration was investigated, and the interaction between miR-183 and PTEN in BC cells was examined. (nature.com)
  • While several studies addressed how cell motility parameters change throughout the cell cycle, the relationship between invadopodia and cell cycle progression has not been elucidated. (biorxiv.org)
  • Cell cycle progression is faster in cells in which invadopodia are abolished (by Tks5 knockdown), evidenced by earlier induction of cyclins A and B. A close look at the regulators of G1 revealed that the overexpression of p27 kip1 , but not p21 cip1 , causes faster turnover of invadopodia and increased ECM degradation. (biorxiv.org)
  • Taken together, these findings suggest that invadopodia function is tightly linked to cell cycle progression and is controlled by specific cell cycle regulators. (biorxiv.org)
  • Cell cycle progression is governed by a complex network of cyclin-dependent kinases that define not only the phase of the cell cycle, but also the timing of transitions between phases 13 . (biorxiv.org)
  • Using gene manifestation profiling, practical siRNA library testing and reverse-phase protein arrays (RPPA), we buy Dihydrotanshinone I found that J2 cells and Y produced a cooperative effect on human being foreskin keratinocytes (HFKs), which resulted in suppression of TGF- signaling juxtaposed with up-regulation of changes in cell cycle progression, cell adhesion and motility. (cell-metabolism.com)
  • [ 14 ] Subsequent work in our laboratory demonstrated that K17 serves as a nuclear shuttle of p27KIP1, resulting in sustained cell cycle progression. (medscape.com)
  • The remaining follicular cells develop into a corpus luteum that produces E and progesterone (P). In turn, P promotes more proliferation within the uterus and vagina, and cornification in the vagina. (biomedcentral.com)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
  • During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
  • During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
  • This research aimed to determine the levels of IL-22, myeloperoxide (MPO), proliferation and wild-type p53 expression after the administration of DPE to murine models of CAC. (biomedcentral.com)
  • Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. (biomedcentral.com)
  • These findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. (biomedcentral.com)
  • Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
  • In order to investigate changes resulting from exposure to tensile stretch, we first looked at changes in proliferation and expression of a few cellular markers associated with epithelial-mesenchymal transition (EMT). (ku.edu)
  • We found that a tensile force significantly reduces cellular proliferation and causes significant shifts in cell cycle distribution. (ku.edu)
  • MTT assays were employed to determine the effect of cell proliferation. (biomedcentral.com)
  • Meanwhile, overexpression of ENDOD1 suppressed cell proliferation, induced G0/G1 cell cycle arrest and inhibited cell migration and invasion. (biomedcentral.com)
  • Conversely, siRNA-mediated silencing of ENDOD1 promoted cell proliferation, migration and invasion. (biomedcentral.com)
  • To explore the effect on proliferation, the researchers used different concentrations of ivermectin on the cultured cells and found cell viability decreased in a dose-dependent and time-dependent manner. (freelysocial.com)
  • Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
  • in turn, the malignant epithelial cells can undergo reversible epithelial-mesenchymal transitions ( 1 - 5 ). (aacrjournals.org)
  • Launch Increasing evidence works with a role of the epithelial to mesenchymal changeover (EMT) procedure in endowing subsets of tumor cells with properties traveling malignant tumor development and level of resistance to cancers therapy. (researchtoactionforum.org)
  • Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
  • In adenocarcinomas, K17 staining was detected in a mean of 33.9% of malignant cells. (medscape.com)
  • Blood cell DNA methylation biomarkers in preclinical malignant pleural mesothelioma: The EPIC prospective cohort. (who.int)
  • In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
  • Myofibroblast activation and partial epithelial-mesenchymal transition (pEMT) are the key steps of fibrosis that synthesize and deposit extracellular matrix and progressively destroy kidney structure [ 3 , 4 ]. (nature.com)
  • Strikingly, histone deacetylase (HDAC) inhibition markedly reduced cell viability by inducing a dose-dependent G0/1 arrest and led to mesenchymal-epithelial transition as evidenced by morphological change and increased E-cadherin and β-catenin expression. (por-journal.com)
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • LF82 also potentiated TGF-β1-stimulated epithelial-mesenchymal transition and myofibroblast activation in IECs, by further inducing gene expression of the main mesenchymal cell markers FN1 and VIM and downregulating the IEC marker OCLN . (biomedcentral.com)
  • These cells not only derive from resident mesenchymal cells (fibroblasts and smooth muscle cells) but can also originate from epithelial and endothelial cells via epithelial/endothelial transition, as well as from stellate cells, pericytes and bone marrow stem cells [ 5 ]. (biomedcentral.com)
  • Expression of markers of myofibroblast activation was determined in TGF-β1-stimulated human intestinal epithelial cells (IECs). (biomedcentral.com)
  • Sustained inflammation and chronic wound healing response often lead to intestinal fibrosis, a condition defined by an excessive accumulation of extracellular matrix (ECM) proteins produced by activated myofibroblasts, which are alpha smooth muscle actin (α-SMA)-expressing cells. (biomedcentral.com)
  • The drug is metabolized by enzymes of the intestinal microflora, hepatocytes, and other cells. (closerpharmacy.com)
  • It is metabolized by enzymes of the intestinal microflora, hepatocytes and other cells with the formation of active metabolite - pollutant (inhibitor of dihydrofolate reductase and timdelaghetto). (closerpharmacy.com)
  • Therefore, this study mainly explored whether Trichinella spiralis Kazal-type serine protease inhibitor (TsKaSPI) contributed to the invasion of intestinal epithelial cells during the infectious stage of T. spiralis by regulating ERS. (biomedcentral.com)
  • Although studies of ERS have become a hot spot, there have been no relevant studies on the relationship between TsSPI and ERS in intestinal epithelial cells. (biomedcentral.com)
  • The labelling patterns reflected strain-dependent carbon flux via glycolysis and/or the Entner-Doudoroff pathway, the pentose phosphate pathway, the TCA cycle and anapleurotic reactions between PEP and oxaloacetate. (uni-muenchen.de)
  • In UCS, TP53 has been identified as the most frequently mutated gene, followed by mutations in the PI3K pathway, KRAS , cell cycle regulators including FBXW7 and chromatin remodeling and histone genes including ARID1A [ 7 - 9 ]. (por-journal.com)
  • Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • TGFβ may exert cytostatic results via effector hands downstream of the canonical Smad2/3 pathway in normal cells. (researchtoactionforum.org)
  • The canonical TGFβ pathway was triggered in Py2T cells upon TGFβ treatment indicated from the nuclear translocation of the Smad2/3 complex and the activation of Smad3 by phosphorylation (Number S1A). (researchtoactionforum.org)
  • Some SARS-CoV-2 variants, such as Omicron, also infect the host cell through the endocytic pathway. (news-medical.net)
  • We have used stroma-rich models in immunodeficient mice, which recapitulate the histotypic arrangement found in human epithelial cancers. (aacrjournals.org)
  • Specifically Py2T cells may serve to corroborate latest results relating EMT to cancers cell stemness to therapy level of resistance also to tumor recurrence. (researchtoactionforum.org)
  • Using this strategy, we identified Ciclopirox, an antifungal drug, as a potent inducer of lytic cycle in EBV+ epithelial cancers. (purdue.edu)
  • Conveniently Py2T LT cells maintained their mesenchymal phenotype even when freezing and re-cultured in the presence of TGFβ. (researchtoactionforum.org)
  • The characterization of the cultured AT2 cells complies with current criteria for an alveolar type 2 cell phenotype. (biomedcentral.com)
  • Additionally, cells have been identified with a keratin phenotype intermediate between basal and luminal cells that co-express high levels of K5 and K18 (K5/18) as well as hepatocyte growth factor receptor c-MET. (johnshopkins.edu)
  • The objective of this study was to further clarify the phenotype of the proposed intermediate cells in PIA and to quantitatively determine the level in which these intermediate cells preferentially occur in PIA lesions. (johnshopkins.edu)
  • Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
  • In this study, we determined the protein expression of ENDOD1 in PCa tissues by immunohistochemistry (IHC) and explored its correlations with clinicopathological parameters. (biomedcentral.com)
  • However, when cells in tissues are sequenced in bulk, rare cells are diluted and averaged out. (technologynetworks.com)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • The kidney tissue is determined in several weeks and the tissues of the liver, for three months. (closerpharmacy.com)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • Launch Epithelial to mesenchymal changeover (EMT) can be an embryonic mobile program where polarized epithelial cells eliminate their cell-cell adhesions and convert right into a motile mesenchymal cell type [1] [2]. (researchtoactionforum.org)
  • These conditionally reprogrammed epithelial cells indicated markers of adult stem cells, but not of embryonic and induced pluripotent stem cells, and appeared to consist of cell populations resembling their main tissue of source, making them ideal to study tissue regeneration, as well as normal physiologic and pathologic processes [8]. (cell-metabolism.com)
  • however, because recombinant viruses that lack the G protein have still been able to replicate in vitro and in vivo, it seems that attachment via the G protein is not required for rest of the replication cycle. (wikipedia.org)
  • Viability assays and cell cycle analyses were used to test in vitro sensitivity to different standard and novel treatments. (por-journal.com)
  • Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
  • Research using in vitro cell cultures are frequently conducted under static growth conditions. (ku.edu)
  • Since human monocyte-derived macrophages (HMDMs) do not express ACE2, these cells were used as in vitro models for ACE2-negative macrophages. (news-medical.net)
  • Therefore, using primary human AT2 cells instead of cell lines for in vitro experiments has become desirable. (biomedcentral.com)
  • A549 epithelial cells) for in vitro experiments has become desirable. (biomedcentral.com)
  • An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
  • Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
  • With that being said, it has been determined that the first step of the hMPV replication cycle is attachment to the host cell, specifically the epithelial cells of the respiratory tract, using the G protein. (wikipedia.org)
  • ALDH3A1, a detoxifying enzyme, is recruited in the corneal epithelium as a crystalline protein with additional functions beyond its metabolic role, which remain to be determined. (europa.eu)
  • The (13)C-isotopologue patterns of protein-derived amino acids from the bacteria and the host cells were then determined by mass spectrometry. (uni-muenchen.de)
  • Our work bridges protein electrostatics and structure with cell biology to reveal how pHi dynamics regulates cell behaviors through protonation of titrating amino acids as a post-translational modification to regulate protein structure and function (Schönichen et al. (ucsf.edu)
  • The high impact our pioneering work bridging protein electrostatics and cell biology is highlighted by our publication h-index of 46. (ucsf.edu)
  • These results indicate that a mechanically active culture environment does impact cell survival and protein expression, and has important implications in the context of the discovery and screening of new antitumor drug therapies. (ku.edu)
  • Real-time PCR and western blot were used to detect ENDOD1 mRNA and protein expression in normal prostatic epithelial and PCa cell lines. (biomedcentral.com)
  • Western blot showed that androgen-sensitive LNCaP cells had the highest protein expression level of ENDOD1, whereas castration-resistant PCa cell lines PC3 and DU145 had significantly lower protein levels. (biomedcentral.com)
  • The SARS-CoV-2 spike (S) protein infects host epithelial cells by binding to the angiotensin-converting enzyme 2 (ACE2) and subsequently fusing the membranes. (news-medical.net)
  • There are changes in Timpilo acid and purine, which in turn changes the connection of DNA and division of cells, the connection of RNA and protein. (closerpharmacy.com)
  • Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells. (uni-muenchen.de)
  • Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, was not significantly affected by the infection with either of these intracellular pathogens. (uni-muenchen.de)
  • Generally, after SARS-CoV-2 infects the epithelial cells of the respiratory tract, antiviral and proinflammatory cytokines are released. (news-medical.net)
  • Epithelial cell cultures were established from lung tissue obtained from patients undergoing diagnostic or therapeutic video-assisted thoracoscopic surgery (VATS) performed at the Division of Thoracic Surgery or undergoing flexible bronchoscopy with transbronchial biopsy at the Clinics of Respiratory Medicine, University Hospital Basel, Switzerland. (biomedcentral.com)
  • This technique was employed recently to examine the differential drug response of cells acquired from a patient with recurrent respiratory papillomatosis to identify a drug that was effective in controlling this disease [9]. (cell-metabolism.com)
  • After hPMSC transplantation, the AMH and FSHR expression in ovarian tissue was significantly higher than in the POF group as determined by immunochemistry and western blot analysis. (biomedcentral.com)
  • [ 15 ] Other studies have shown K17 to be a negative prognostic indicator in gastric adenocarcinoma, bladder carcinoma, epithelial ovarian carcinoma, and triple-negative breast carcinoma. (medscape.com)
  • We hypothesize that tensile (mechanical) forces applied to two non-small cell lung cancer cell lines, bronchoalveolar H358 cells and alveolar A549 cells, play an important role in determining cellular response to chemotherapeutic agents. (ku.edu)
  • In this study, we analysed the intracellular carbon metabolism of enteroinvasive Escherichia coli (EIEC HN280 and EIEC 4608-58) and Salmonella enterica Serovar Typhimurium (Stm 14028) replicating in epithelial colorectal adenocarcinoma cells (Caco-2). (uni-muenchen.de)
  • To test the influence ivermectin may have on colorectal cancer cells, the team used cancer cell lines SW480 10 and SW1116. (freelysocial.com)
  • Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
  • Although intermediate cells have been proposed as precursor cells of prostate cancer, their biology is ill defined. (johnshopkins.edu)
  • Thus, macrophages not only inhibit viral dissemination but can sense infected cells and infectious viral particles to subsequently elicit antiviral and proinflammatory programs. (news-medical.net)
  • This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
  • siRNA screening The siRNA library to assess factors secreted by J2 cells was comprised of 332 fully annotated genes based on the 3T3 cell transcriptome in GEO database "type":"entrez-geo","attrs":"text":"GSM1348503″,"term_id":"1348503″GSM1348503. (cell-metabolism.com)
  • While the majority of LCLs comprised cells containing EBV in the latent phase of its life cycle, we identified two additional clusters that had distinct expression of both host and viral genes. (purdue.edu)
  • In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
  • Immortalized human lung epithelial cells, BEAS-2B, were exposed to all MW for 24 hours. (cdc.gov)
  • However, this preliminary evaluation indicates that human lung epithelial cells exposed to AP-MW and their coated counterparts produces a genotoxic response. (cdc.gov)
  • Here we present a technique to isolate primary human AT2 cells by sprouting directly from peripheral human lung tissue. (biomedcentral.com)
  • Clinical signs did not develop in any of porcine turbinate cells (PT-K75) and primary chicken animal, nor were gross pathologic changes evident on embryo epithelial kidney cells supported SARS-CoV repli- postmortem examinations. (cdc.gov)
  • Vero V76 and porcine turbinate cells PT-K75, seeded at a lose (Sigma, St. Louis, MO)/ Dulbecco modified Eagle density of 2 x 105 cells/cm2 in 12-well plates (Costar) 24 h medium (DMEM) (Wisent, St. Bruno, Quebec), and incu- before inoculation. (cdc.gov)
  • Background: Renal infiltration of inflammatory cells including macrophages is a crucial event in kidney fibrogenesis. (bvsalud.org)
  • The recent development of a human kidney cell atlas will help researchers better understand the factors contributing to disease states and provide a critical foundation to help discover new treatments for acute kidney injury and chronic kidney disease. (hospitalhealthcare.com)
  • In the study, published in the journal Nature , a team of US researchers developed a kidney tissue atlas consisting of 51 main cell types including rare and novel cell populations and 28 cellular states indicative of injury or disease. (hospitalhealthcare.com)
  • It was created from 45 healthy donor organs and 48 kidney disease biopsies and allows for the comparison of healthy and injured cells. (hospitalhealthcare.com)
  • With the data, the researched were able to define 28 cellular states across nephron segments and interstitium that were altered in kidney injury, encompassing cycling, adaptive (successful or maladaptive repair), transitioning and degenerative states. (hospitalhealthcare.com)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Transwell migration and invasion assays were done to evaluate changes in the ability of cell migration and invasion. (biomedcentral.com)
  • To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)
  • We previously demonstrated that invasive cells oscillate between two distinct states, termed the Invadopodia state and the Migration state 10 . (biorxiv.org)
  • The Invadopodia state is characterized by cell stasis and the presence of invadopodia, whereas the Migration state is defined by cell translocation and the absence of invadopodia. (biorxiv.org)
  • The molecular mechanisms mediating pHi-regulated cell behaviors, however, remain understudied and largely unknown. (ucsf.edu)
  • The underlying mechanisms of the SARS-CoV-2 replication cycle have been elucidated using various epithelial cell lines. (news-medical.net)
  • Only recently, the role of impaired epithelial repair mechanisms after injury in the pathogenesis of idiopathic pulmonary fibrosis has been demonstrated, and has shifted the AT2 cell in the focus of interest. (biomedcentral.com)
  • The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
  • The molecular mechanisms governing EBV carcinogenesis remain elusive and the functional interactions between virus and host cells are incompletely defined. (purdue.edu)
  • We used EBV+ GC cells to determine the effect of Ciclopirox on EBV reactivation as well as identify the underlying mechanisms. (purdue.edu)
  • however, mechanisms through which these bioactive compounds act on epithelial cells have not been identified. (biorxiv.org)
  • [ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
  • This was accompanied by enhanced gene expression of proinflammatory cytokines and chemokines, as well as more recruited inflammatory cells into the intestine. (biomedcentral.com)
  • Activation of myofibroblasts occurs in response to different stimuli, including growth factors such as transforming growth factor beta (TGF-β) and platelet-derived growth factor (PDGF), pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-13 and IL-17, as well as CC chemokines like CCL2, CCL3 and CCL4, and lipid mediators released by immune and non-immune cells [ 6 ]. (biomedcentral.com)
  • Researchers in Australia investigated the cell type that can effectively sense SARS-CoV-2 and trigger the release of antiviral and inflammatory cytokine mediators. (news-medical.net)
  • Epithelial cells in proliferative inflammatory atrophy (PIA) appear to be cycling rapidly as indicated by expression of Ki-67, and morphological transitions have been identified between PIA and high-grade prostate intraepithelial neoplasia. (johnshopkins.edu)
  • HFKs were co-cultured with irradiated (3,000 Rad) J2 cells (kindly provided by Dr. Richard Schlegel, Georgetown University or college) derived from mouse NIH 3T3 fibroblasts at a 1:4 HFK:J2 cell percentage, and sub-cultured when they reached 85% confluence. (cell-metabolism.com)
  • SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)
  • Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). (medscape.com)
  • Cervical cancer has multiple histologic subtypes, with squamous cell carcinoma (SCC) accounting for approximately 75% of cases and invasive adenocarcinoma accounting for 10% to 15% of cases. (medscape.com)
  • [ 11-13 ] By contrast, we discovered, using laser capture microdissection and mass spectrometry, that keratin 17 (K17) could predict the overall survival of patients with cervical squamous cell carcinoma more accurately than grade, stage, or any other clinicopathologic features. (medscape.com)
  • PF338 tumor cells were resistant to MEK- and TGF-β signaling-inhibition but sensitive to PIK3CA- and PARP-inhibition and first-line chemotherapeutics. (por-journal.com)
  • In keeping with the metastatic function of the EMT recent outcomes have got indicated that EMT confers stem cell-like features to tumor cells [10]-[12]. (researchtoactionforum.org)
  • However, under these microenvironmental conditions, only 15% of tumor cells assemble invadopodia at any given time, while the rest are non-motile. (biorxiv.org)
  • Several alkylating agents are commonly used in chemotherapy but may induce DNA damage to cancer cells as well as normal cells. (frontiersin.org)
  • Chemotherapy, either at conventional levels or in the higher-dosed myeloablative protocols used in conditioning regimens (with or without total body radiation in preparation for hematopoietic cell transplantation [HCT]), often results in erythema, edema, atrophy, and ulceration of the oral mucosa, a condition generally referred to as oral mucositis. (medscape.com)
  • Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
  • As confirmed by immunoblotting analysis Py2T LT cells displayed a lack of E-cadherin manifestation along with high manifestation of BIIE 0246 the mesenchymal markers N-cadherin and fibronectin (Number 1F). (researchtoactionforum.org)
  • In this study, using invadopodia- and cell cycle- fluorescent markers, we show in 2D and 3D cell cultures, as well as in vivo , that breast carcinoma cells assemble invadopodia and invade into the surrounding ECM preferentially during the G1 phase of the cell cycle. (biorxiv.org)
  • In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)
  • Primary cilia are assembled on different types of human cells depending on their state and activities in response to cellular quiescence where they relay extracellular signals and retract upon cell cycle re-entry [ 5 ]. (ersjournals.com)
  • NRK-49F cells were seeded on macrophage-derived extracellular matrix (ECM) scaffold. (bvsalud.org)
  • Because of its mutated aggressive genetics, this cell has a selective growth advantage over its neighbors. (medscape.com)
  • We conclude that cells phenotypically intermediate between basal and secretory cells are enriched in PIA lesions. (johnshopkins.edu)
  • K17 was not detected in the epithelial cells of benign glandular lesions, but groups of cuboidal cells, residing beneath the epithelial layer of benign glands, were frequently positive for K17, especially in cases of microglandular hyperplasia. (medscape.com)
  • Several studies have determined that the extent of tubulointerstitial involvement is correlated better with renal function deterioration than glomerular changes. (nature.com)
  • In this project, we have used an intuitive in-silico drug prediction approach to rapidly screen and identify FDA-approved or clinically available compounds that can be repurposed to induce lytic cycle in different EBV+ tumors. (purdue.edu)
  • Researchers applied multiple single-cell and single-nucleus assays and spatial imaging technologies to both healthy and diseased kidneys. (hospitalhealthcare.com)
  • Epithelial cell cultures were established from lung tissue obtained from patients undergoing diagnostic or therapeutic video-assisted thoracoscopic surgery or undergoing flexible bronchoscopy with transbronchial biopsy. (biomedcentral.com)
  • PTEN insufficiency promotes the PI3K/AKT path, which is a vital signal for the survival of cancer cells. (nature.com)
  • The bile ducts carry bile, a fluid that is produced by the liver cells (hepatocytes) and modified by the biliary lining (epithelial) cells as it flows through the ducts to the small intestine. (emedicinehealth.com)
  • As the inflammation continues to destroy more of these bile ducts, it spreads to destroy nearby liver cells (hepatocytes). (emedicinehealth.com)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
  • The results showed that hPMSC transplantation can significantly recover the estrus cycle in the POF group. (biomedcentral.com)
  • mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
  • Cells synchronized in the G0/G1 phase of the cell cycle degrade at significantly higher levels during the first 20 hours post-synchronization release. (biorxiv.org)
  • In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
  • Preliminary two-color cell cycle analysis showed no evidence of cell cycle disruption after 24 hour treatment to 2.4 microg/cm2 MW. (cdc.gov)
  • Outcomes Py2T a Book Breast Cancer tumor Cell Line Going through TGFβ-induced EMT To determine a mobile model system that might be used to review epithelial to mesenchymal changeover (EMT) and in addition EMT in Py2T cells. (researchtoactionforum.org)
  • species during the first week after inoculation, and in chick- en organs at 2 weeks after inoculation. (cdc.gov)
  • We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
  • Within the human prostate epithelium four cell populations can be discriminated based on their expression of keratins (K). Basal cells express high levels of K5 and K14, as well as p63, whereas they have very low levels of androgen receptor, prostate-specific antigen (PSA), K8, and K18. (johnshopkins.edu)
  • Using radical prostatectomy specimens (n = 15) the area fraction of intermediate cells in normally differentiated prostate epithelium and PIA were quantified by a grid point counting method. (johnshopkins.edu)
  • Although p27 Kip1 was strongly expressed in K5-negative differentiated cells in normal epithelium, p27 Kip1 staining was absent in many of the K5-positive cells in the luminal compartment of PIA. (johnshopkins.edu)
  • To unambiguously determine whether intermediate luminal cells in PIA show increased proliferative activity and decreased p27 kip1 expression, double-staining immunofluorescence of Ki-67 and K5, as well as p27 Kip1 and K5 was performed. (johnshopkins.edu)
  • The ivermectin also altered cell morphology, demonstrating a decrease in cells after just 24 hours and a loss of their original shape. (freelysocial.com)
  • Several groups have developed methods to isolate human AT2 cells applying tissue digestion and consecutive filtration in their protocols. (biomedcentral.com)
  • Materials and Methods Cells Human being keratinocytes were cultured from discarded and de-identified neonatal foreskin cells. (cell-metabolism.com)
  • But because it is still a relatively new virus and has not yet been researched very heavily, hMPV and its replication cycle still have a lot of mystery surrounding them. (wikipedia.org)
  • However, researchers have been able to elucidate some principal steps of hMPV's replication cycle, basing their approach and experimentation on the current knowledge we have of the viral life cycles and reproductive measures of the rest of the Paramyxoviridae family. (wikipedia.org)
  • In contrast to epithelial cells, human macrophages manifest an abortive replication cycle for many viruses, such as rhinovirus and seasonal influenza A viruses. (news-medical.net)
  • Whereas immunohistochemistry of the metastatic tumor showed a predominantly sarcomatous vimentin positive tumor that has lost E-cadherin expression, PF338 cells demonstrated biphasic growth and carried mutations in KRAS , PIK3CA , PTEN and ARID1A . (por-journal.com)
  • In UCS, several studies identified characteristic EMT-related expression signatures including active TGF-β signaling in tissue and cell lines [ 7 , 11 , 13 , 14 ]. (por-journal.com)
  • Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
  • Real-time PCR data indicated that ENDOD1 mRNA expression was markedly increased in LNCaP and 22Rv1 cells and decreased in PC3 and DU145 cells compared to the normal epithelial cells RWPE1. (biomedcentral.com)
  • For instance, single-cell RNA, assay for transposase-accessible chromatin (ATAC) and chromosome conformation (Hi-C) sequencing offers information on gene expression profiling, accessible chromatin regions and chromatin contacts, respectively. (technologynetworks.com)
  • Second, we determined that the expression of twelve EBV transcripts, including LMP1 and LMP2, correlated inversely with EBV reactivation signature. (purdue.edu)
  • Many of the atrophic epithelial luminal cells in PIA are candidates for intermediate cells based in part on weak expression of PSA and androgen receptor, high levels of K8/18, and lack of p63. (johnshopkins.edu)