• 3. Glycosaminoglycans modulate C6 glioma cell adhesion to extracellular matrix components and alter cell proliferation and cell migration. (nih.gov)
  • 14. Modulation of growth factor responsiveness of murine mammary carcinoma cells by cell matrix interactions: correlation of cell proliferation and spreading. (nih.gov)
  • Lastly, we observe a decrease in proliferation in cells transfected with miR-200c mimic, and cells where ZEB1 is knocked down stably, demonstrating that the ability of miR-200c to enhance sensitivity to paclitaxel is not due to an increased proliferation rate. (hindawi.com)
  • Airway epithelial cell migration and proliferation capacities on normal and stiff substrates were evaluated through video-microscopy and flow cytometry. (frontiersin.org)
  • The results showed that migration and proliferation of ITGB4 deficiency cells were noticeably inhibited, along decreased cytoskeleton stabilization, and hampered RhoA activity, especially for cells cultured on the stiff substrate. (frontiersin.org)
  • The ability of SIS to support epidermal cell/fibroblast attachment, migration and/or proliferation and differentiation with deposition of basement membrane (BM) components indicates that the composite model may be useful for studying cell-matrix interactions and for investigation as a dermal substitute. (usuhs.edu)
  • other neoplastic disorders can cause local tissue infiltration without metastasis such as desmoid tumours which are mesenchymal tumours characterised histologically by fibroblast proliferation and marked production of collagen 13 . (ersjournals.com)
  • Communication between cancerous and surrounding cells is a two-way process and engages a diverse range of mechanisms that, in consequence, can lead to rapid proliferation, metastasis, and drug resistance, or can serve as a tumors-suppressor, e.g., through tumor-immune cell interaction. (encyclopedia.pub)
  • Each of these stromal cell types plays a role in tumor proliferation, metastasis, and treatment failure [ 4 ] [ 5 ] . (encyclopedia.pub)
  • The extracellular matrix (ECM) is a highly dynamic structure that surrounds the above-mentioned cells and affects their proliferation and cell-cell communication via the transmission of mechanical signals and cell adhesion [ 6 ] . (encyclopedia.pub)
  • They not only provide the scaffold for cells and cell layers, but they also play an essential role in morphogenesis, which affects cell adhesion, migration, proliferation, and differentiation. (grantome.com)
  • The 'seeding' theory demonstrates that several consecutive steps participate in the development of peritoneal dissemination, including detachment from the primary tumour, transmigration to the peritoneum, attachment to peritoneal mesothelial cells or lymphatic stomata, invasion through the basement membrane to subperitoneal tissue and proliferation with blood vascular neogenesis ( Fig. 1 ) ( 7 ). (spandidos-publications.com)
  • 6) Proliferation with infiltration of stromal cells, particularly CAF (CXCL12/CXCR4). (spandidos-publications.com)
  • Tumor microenvironment (TME) Oncology research solutions TABLE OF CONTENTS Molecular drivers of cancer pathogenesis Cancer progression is associated with the interplay between tumor cells and the surrounding environment, which requires signal transduction pathways to relay messages throughout the cell.1,2 Cell signaling pathways regulate everything from cell growth to proliferation to survival. (technologynetworks.com)
  • Many of the genes commonly mutated encode Purpose and scope INTRODUCTION components or targets of the PI3K/AKT and Ras/ERK pathways, causing dysregulation of cellular signaling.1 This dysregulation drives cancer progression by influencing the behavior of tumor cells through cell proliferation, survival, migration, differentiation, metabolism, polarity, angiogenesis, and the tumor microenvironment. (technologynetworks.com)
  • Specifically inhibits endothelial cell proliferation, migration and tube formation. (cusabio.com)
  • Col4a1 and Col4a2 mutants displayed disrupted myofibroblast proliferation, differentiation and migration. (cusabio.com)
  • MAPK) signaling augment cell growth and proliferation in melanoma and other solid tumors.1,2 Both clinical and translational research focuses on exploration of the MAPK signaling pathway to detect predictors of resistance and response. (globaltechbiz.com)
  • The changes include the proliferation and dilatation of epithelial ducts in these tissues with abnormal deposition of extracellular matrix. (bmj.com)
  • They include the proliferation and dilatation of epithelial ducts, and proliferative changes in the extracellular matrix of stromal connective tissue. (bmj.com)
  • Ca 2+ is an important second messenger which mediates vital cellular functions from cell proliferation to cell death. (biomedcentral.com)
  • It arises from the uncontrolled proliferation of epithelial cells or cells exhibiting cytological or tissue architectural characteristics of SC differentiation, including the presence of keratin, tonofilament bundles or desmosomes, which are structures involved in cell-cell adhesion ( 1 ). (spandidos-publications.com)
  • [ 15 ] Matrigel, however, in his view, is a very poor substitute of the BM that is usually built by type IV collagen, laminin, and entactin organized in a complex, covalently bound network. (medscape.com)
  • Basement membrane extracts (BME) are crude ECM mixtures that may include laminin, collagen IV, entactin, and heparan sulfate proteoglycan, and which are isolated from ECM-rich Engelbreth-Holm-Swarm (EHS) sarcoma tumors in mice. (sigmaaldrich.com)
  • Basement membranes consist of laminin, collagen IV, perlecan, nidogen/entactin, and other molecules, which interact with one another to form the supramolecular structure. (grantome.com)
  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. (cusabio.com)
  • Geltrex matrix is a basement membrane extract that contains laminin, collagen IV, entactin, and heparin sulfate proteoglycans. (thermofisher.com)
  • During the discussion of this somehow "iconoclastic" paper (as acknowledged by Dr. Hotary himself), [ 14 ] he added that the input of fibroblasts and endothelial cells was not evaluated in these models, as these cell types are not usually present in these systems, and they were not added to the cultures. (medscape.com)
  • ECM Gel supports numerous in vitro assays such as cell adhesion, neurite outgrowth, cell invasion, cell migration, and angiogenesis tube formation with multiple cell types including epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells, and stem cells. (sigmaaldrich.com)
  • Basement membranes are thin extracellular matrices that separate epithelial and mesenchymal cells, and surround cells such as endothelial, muscular, and neural cells. (grantome.com)
  • In addition, we detected MMP-8 mRNA expression in cultured rheumatoid synovial fibroblasts and human endothelial cells. (uni-bielefeld.de)
  • However, other cell types in the heart, including endothelial cells and stroma plays an important role in the development of heart disease, response to injury and cardiomyocyte function. (celulastroncales.org)
  • In addition, Geltrex matrix is tested for its ability to support endothelial tube formation from cryopreserved endothelial cells. (thermofisher.com)
  • In addition, it can be used for the promotion and maintenance of a differentiated phenotype in a variety of cell cultures including primary epithelial cells, endothelial cells, glandular cells, neurons, and smooth muscle cells. (thermofisher.com)
  • We show here that exposure of expansively growing human WM852 melanoma cells, but not singly invasive Bowes cells, to lymphatic endothelial cells (LEC) in 3D co-culture facilitates melanoma distant organ metastasis in mice. (elifesciences.org)
  • C1q is also synthesized by decidual endothelial cells, thus acting as a link between decidual cells and trophoblasts, as well as contributing to the remodelling of spiral arteries. (explorationpub.com)
  • Furthermore, the endothelial cells of spiral arteries synthesize C1q exploiting it as a bridge between them and EVTs, which place themselves in the walls of spiral arteries and cause the remodelling of the vessel [ 7 ]. (explorationpub.com)
  • This process involves the migration, growth, and differentiation of ENDOTHELIAL CELLS, which line the inside wall of blood vessels. (nih.gov)
  • Type 1 hemidesmosomes are found in stratified and pseudostratified epithelial tissue. (wikipedia.org)
  • Cell and Tissue Research. (wikipedia.org)
  • In conclusion, Dr. Hotary suggested that "the role of the type IV collagenases MMP 2 and 9 should be re-evaluated" as the newly gathered evidence does not seem to support a critical role of these MMPs in tissue invasion by tumor cells. (medscape.com)
  • In accordance with our previous studies regarding the functions of ITGB4 in bronchial epithelial cells (BECs), we hypothesize that the decreased ITGB4 expression during embryonic stage leads to abnormal ECM remodeling and increased tissue stiffness, thus impairing BECs motility and compromising lung development. (frontiersin.org)
  • These results suggest that decreased ITGB4 expression results in increased lung tissue stiffness and impairs the adaptation of bronchial epithelial cells to substrate stiffness, which may be related to the occurrence of broncho pulmonary dysplasia. (frontiersin.org)
  • Much of our understanding of epithelial wound healing comes from monolayers of epithelial cells grown in tissue culture, which can be imaged at high resolution. (biomedcentral.com)
  • Without controlled migration no tissue, no organ can be formed. (uncommondescent.com)
  • The extracellular matrix (ECM) is an intricate network composed of an array of multidomain macromolecules organized in a cell/tissue-specific manner. (uncommondescent.com)
  • Laminin isoforms show tissue- and developmental stage-specific expression and play important roles in the structures and functions of basement membranes. (grantome.com)
  • The interactions of laminins with cells are essential for laminin-mediated biological activities in tissue development and adult tissue functions. (grantome.com)
  • Perl interacts with many extracellular molecules and cell surface receptors, and is implicated in many biological functions in tissue homeostasis and diseases. (grantome.com)
  • It also explores how dysregulation can lead to pathogenesis with examples across a broad range of cell and tissue types. (technologynetworks.com)
  • This paper is a systematic review of the literature on the mechanisms of cell adhesion to bone grafts associated to nanotechnology, describing the importance and the role of those molecules in the adhesion and thus in tissue regeneration. (bvsalud.org)
  • Accurate identification of these mechanisms of cell adhesion is essential for further advancement in tissue engineering, such as the production of biological bone substitutes that achieve a better clinical outcome. (bvsalud.org)
  • Tissue engineering is a strategy very used to obtain functional repairing through the development of biological substitutes that can restore, keep or substitute damaged tissues or organs 31 , through the combination of scaffolds biocompatible with live cells and/or bioactive molecules 19 . (bvsalud.org)
  • These properties will act mediating the action of cell adhesion proteins, regulating cell behavior and causing tissue regeneration. (bvsalud.org)
  • Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)
  • Mechanisms and pneumococcal factors that enable host epithelial and tissue barriers to be breached during the progression from colonization to invasive infection are still poorly understood. (biomedcentral.com)
  • This review discusses recent advances in hPSCs differentiation of stromal cells to the heart, the identification and classification of stromal cell types of the heart, and application hPSC derived stromal cells of the heart and of tissue containing these cells in regenerative applications and drug development. (celulastroncales.org)
  • Whether you want to stretch cells in Monolayer (2D) or create Tissue Constructs in (3D) cell culture, we have the disposable solution to meet your needs! (flexcellint.com)
  • We invite you to read more about our ongoing research and development on cell stretching bioreactors in tissue engineering, cellular mechanics, gene and protein expression, cytomechanics, drug discovery, orthopedics, cardiovascular, and pulmonary research. (flexcellint.com)
  • Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells. (stanford.edu)
  • We found that the human FBR is mediated by immune-cell-specific RAC2 mechanotransduction signalling, independently of the chemistry and mechanical properties of the implant, and that a pathological FBR that is human-like at the molecular, cellular and tissue levels can be induced in mice via the application of human-tissue-scale forces through a vibrating silicone implant. (stanford.edu)
  • Immediately following tissue wounding, intracellular Ca 2+ concentration ([Ca 2+ ] i ) increases in the cells located at the edge of the wound. (biomedcentral.com)
  • We evaluated the effect of four ECM proteins upregulated in breast tumor tissue in multiple human breast cancer cell lines in three assays. (aip.org)
  • Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. (nih.gov)
  • MiR-200 family members are therefore thought to be expressed in an epithelial cell-specific manner in normal tissues. (hindawi.com)
  • Invasion of tumor-surrounding tissues by cancer cells is mediated by proteolytic enzymes such as MMPs. (medscape.com)
  • [ 19 ] They may, thus, represent a potentially useful target to reduce tumor perforation of BMs and migration into normal tissues. (medscape.com)
  • Basement membrane extracts provide a coating substrate for numerous cell types and recapitulates the natural ECM environment found in tissues. (sigmaaldrich.com)
  • Cells do not only differentiate: they must form tissues and organs and, in the end, a whole body plan. (uncommondescent.com)
  • How can cells, while differentiating and implementing their specific epigenetic programs, form tissues and organs and organisms? (uncommondescent.com)
  • The main feature that contributes to generate tissues, organs and body plans, together with cell differentiation, is cell migration. (uncommondescent.com)
  • Stem cells or progenitor cells are defined by a capacity for self-renewal and the ability to generate different types of cells (multipotentiality) that are involved in the formation of mature tissues. (intechopen.com)
  • Despite the successful isolation and characterization of stem cells from various tissues, relatively few animal studies have been done to investigate the efficacy of stem cell transplantation. (intechopen.com)
  • When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
  • These research show the importance of synergy in controlling epithelial cell phenotype relevant to tissues system as well as the tool of constructed integrin-specific ECM pieces in helping cell phenotype. (bio2009.org)
  • The aim of regenerative medicine to restore the normal function of diseased or damaged cells, tissues, and organs using a set of different approaches, including cell-based therapies. (celulastroncales.org)
  • and engineering of human pluripotent stem cells (hPSC) -derived heart cells and tissues for applications in the study of cellular therapy and cardiotoxicity. (celulastroncales.org)
  • We comprehensively analyzed the expression patterns, prognostic values, immune cell infiltration and biological functions of CREC family in CRC using publicly accessible databases and further verified the bioinformatic results in human CRC cell lines and CRC tissues. (biomedcentral.com)
  • 5. ECM dependent and integrin mediated tumor cell migration of human glioma and melanoma cell lines under serum-free conditions. (nih.gov)
  • 8. Epidermal growth factor enhancement of HSC-1 human cutaneous squamous carcinoma cell adhesion and migration on type I collagen involves selective up-regulation of alpha 2 beta 1 integrin expression. (nih.gov)
  • 15. Different adhesion properties of highly and poorly metastatic HT-29 colon carcinoma cells with extracellular matrix components: role of integrin expression and cytoskeletal components. (nih.gov)
  • Integrin α6β4 operates as a laminin-332 receptor. (wikipedia.org)
  • BPAG2, or (bullous pemphigoid antigen 2), is a transmembrane protein that exists adjacent to integrins, BPAG2 has domains that bind to plectin, integrin β4 subunit in the cytoplasm and integrin α6 and laminin-332 in the extracellular space. (wikipedia.org)
  • This is caused by mutations in integrin α6β4, laminin 322 and BPAG2. (wikipedia.org)
  • Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. (bvsalud.org)
  • Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types. (bvsalud.org)
  • We also found that laminin-1-mediated clustering of GM1 caused translocation and enrichment of the beta1 integrin in the lipid rafts. (grantome.com)
  • These results suggest that the binding of laminin-1 to GM1 facilitates the formation of a focal microdomain in the lipid rafts, and enhances signal transduction for neurite outgrowth by linking and cooperating with NGF-TrkA and laminin-integrin signaling pathways. (grantome.com)
  • 4) Attachment of gastric cancer cells to the ECM factors secreted by PMCs (integrin). (spandidos-publications.com)
  • Notably, the expansively growing metastatic melanoma cells adopted an invasively sprouting phenotype in 3D matrix that was dependent on MMP14, Notch3 and β1-integrin. (elifesciences.org)
  • These experiments identified proteins called MMP14, Notch3, and β1-integrin as critical to the invasive spread of melanoma cells. (elifesciences.org)
  • Since members of the miR-200 family are responsible for repressing ZEB1 and ZEB2 as well as other mesenchymal genes, these miRNAs are considered "guardians of the epithelial phenotype. (hindawi.com)
  • The extracellular matrix (ECM) provides important cues for directing cell phenotype. (bio2009.org)
  • Data suggest that epithelial cells employ RGD just with sixth is v integrins and screen a significant change toward a mesenchymal phenotype credited, in component, to improved modifying development aspect- account activation and/or signaling likened with cells on the synergy formulated with FnIII910. (bio2009.org)
  • This study uncovers a unique mechanism whereby LEC contact promotes melanoma metastasis by inducing a reversible switch from 3D growth to invasively sprouting cell phenotype. (elifesciences.org)
  • 12. Cell interactions and motility in human lung tumor cell lines HS-24 and SB-3 under the influence of extracellular matrix components and proteinase inhibitors. (nih.gov)
  • In Clytia, the choice between cell crawling and purse string mechanisms of wound healing depends on interactions between the epithelial cells and the basement membrane. (biomedcentral.com)
  • and/or 3) epithelial-mesenchymal interactions/transformation. (ersjournals.com)
  • We further observe a regulatory role of the molecule in pathways associated with PDAC ECM remodeling and tumor-stromal crosstalk, such as INS/IGF-1, RAS/MAPK, laminin interactions and collagen biosynthesis. (biomedcentral.com)
  • All cell migrations happen in the ECM, and are controlled and implemented by the complex interactions between the cells and the ECM. (uncommondescent.com)
  • Those interactions, surprisingly, are not only biochemical (as we usually imagine cell interactions to be), but mainly mechanical. (uncommondescent.com)
  • The cell structures that mediate those interactions between the cells and their ECM are one of the most amazing examples of functional complexity in the cell. (uncommondescent.com)
  • Integrins are cell adhesion molecules that mediate cell-extracellular matrix and cell-cell interactions. (embl-heidelberg.de)
  • 17. Laminin-10 mediates basal and EGF-stimulated motility of human colon carcinoma cells via alpha(3)beta(1) and alpha(6)beta(4) integrins. (nih.gov)
  • Integrins, alpha-dystroglycan (alphaDG), and proteoglycans (PGs) such as syndecans have been identified as major cellular receptors for laminins. (grantome.com)
  • The binding of laminin-1 activates integrins via a conformational change, which promotes neurite outgrowth. (grantome.com)
  • Several molecules are involved in the process of cell adhesion to bone grafts, highlighting the role of integrins, the focal adhesion mechanism, the influence of the collagen matrix, and the activity of alkaline phosphatase in bone matrix formation. (bvsalud.org)
  • Cells interact with their root ECM through transmembrane cell-surface receptors known as integrins, heterodimeric elements composed of transmembrane leader and beta subunits, which are connected to cytoskeletal protein such as talin intracellularly, vinculin, and/or paxillin.1 Integrins bind to ECM elements through particular and multiple synergistic sequences on ECM protein often. (bio2009.org)
  • As a total result, cells can display different phenotypic replies to the same ECM molecule depending on the integrins that join,2,3 which is certainly in convert described not really just by mobile reflection of particular integrins but also by the conformation of the ECM ligand, the availability of particular ligand sequences, and the avidity of particular integrins to contending sites of engagement. (bio2009.org)
  • Many integrins require activation on the cell surface before they can bind ligands. (embl-heidelberg.de)
  • Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. (cancerindex.org)
  • 7. Collagen receptor on T lymphocytes and the control of lymphocyte motility. (nih.gov)
  • The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)
  • This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
  • In collaboration with Dr. Eri Arikawa-Hirasawa's group, we found that laminin-1 bound to GM1 through a carbohydrate moiety and dependent on the conformation of GM1, it induced focal formation of large clusters of GM1, and it enhanced relocation of NGF receptor TrkA into the lipid rafts of dorsal root ganglion (DRG) and PC12 cells. (grantome.com)
  • Of the genes affected by all three arsenicals, only one, that coding for interleukin-1 receptor, type II, showed enhanced expression, a finding confirmed by the reduced increase in NF-κB (nuclear factor kappa B) activity seen in response to interleukin-1β in iAs III -exposed cells. (nih.gov)
  • To occur the adhesion of CAMs to the biomaterial, it is necessary their interaction with some components of extracellular matrix, such as proteoglycans, collagen and proteins 23 . (bvsalud.org)
  • Proteoglycans have a net negative charge that attracts positively charged sodium ions (Na + ), which attracts water molecules via osmosis, keeping the ECM and resident cells hydrated. (knowpia.com)
  • 20. The effect of substrate and adsorbed proteins on adhesion, growth and shape of CaCo-2 cells. (nih.gov)
  • MiRNAs can affect the expression of a large number of proteins, including those involved in pathways relevant to cancer, such as apoptosis, migration and metastatis. (hindawi.com)
  • It now appears clearly that cell-surface proteins participate in many stages of the colonization process and/or the disease transition. (biomedcentral.com)
  • conducted a series of experiments to identify specific proteins in the melanoma cellsthat were responsible for making the cancer more invasive after it interacted with the lymph vessel cells. (elifesciences.org)
  • It occurs as a proteoglycan (PG) in which two or three HS chains are attached in close proximity to cell surface or ECM proteins. (knowpia.com)
  • In the extracellular matrix, especially basement membranes , the multi-domain proteins perlecan , agrin , and collagen XVIII are the main proteins to which heparan sulfate is attached. (knowpia.com)
  • There was no linear relationship between cell adhesion to ECM proteins and ECM-driven 2D cell migration speed, persistence, or 3D invasion. (aip.org)
  • Additionally, talin 1 and laminin alpha 3, which participate in signaling pathways associated with adhesion and migration, have been previously observed to be overexpressed in SCC ( 9 ). (spandidos-publications.com)
  • The examination of ECM components collagen, elastin, and lysyl oxidase (LOX) family showed that the expression of type VI collagen, elastin and LOXL4 were significantly elevated in the ITGB4-deficiency mice, compared with those in normal groups. (frontiersin.org)
  • Our specially designed flexible bottomed 6-well and 24-well BioFlex ® culture plates are offered in a range of protein coatings: Amino, Collagen (Type I and IV), Elastin, Pronectin (RGD), Laminin (YIGSR) that save the researcher time and money. (flexcellint.com)
  • With the increased understanding of molecular mechanisms and basic pathways in the pathogenesis of squamous cell cancer of the head and neck , these pathways may be modified, and rational approaches in cancer therapy at the molecular level may be created. (medscape.com)
  • Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)
  • Hemidesmosomes are also involved in signaling pathways, such as keratinocyte migration or carcinoma cell intrusion. (wikipedia.org)
  • A previous study used proteomic technology to reveal the signaling pathways of antler stem cell derived from red deer. (peerj.com)
  • Finally, downstream nuclear targets of signaling pathways like the transcription factors Myc and NF-κB, chromatin remodelers, and cell cycle effectors are also commonly altered. (technologynetworks.com)
  • The present study aimed to investigate the genes and signaling pathways associated with squamous cell carcinoma (SCC) by bioinformatics analysis. (spandidos-publications.com)
  • DEGs of SCC were primarily enriched in pathways associated with cancer and cell adhesion. (spandidos-publications.com)
  • The well-characterized Madin-Darby canine kidney (MDCK) cells, which give rise to a polarized epithelial epithelium in vitro, lay down a normal BM in culture dishes. (medscape.com)
  • We identified cell crawling and purse string-mediated mechanisms of healing in Clytia epithelium that appear highly analogous of those seen in higher animals, suggesting that these mechanisms may have emerged in a common ancestor. (biomedcentral.com)
  • The epithelium is made up of one or more layers of epithelial cells covering the external surface of the body and lining internal cavities. (biomedcentral.com)
  • Cancer stem cells (CSCs), a small population of cancer cells, have been considered to be the origin of cancer initiation, recurrence, and metastasis. (nature.com)
  • Noncanonical Wnt5-Frizzled2 pathway also regulates epithelial-mesenchymal transition (EMT), a characteristic of CSCs, and promotes metastasis in hepatocellular carcinoma (HCC) and colon cancer cells through the activation of STAT3 10 . (nature.com)
  • Regardless of cell type, the tumorigenic changes that drive cancer growth and metastasis are complex. (technologynetworks.com)
  • Metastasis, the leading cause of death in cancer patients, requires the invasion of tumor cells through the stroma in response to migratory cues, in part provided by the extracellular matrix (ECM). (aip.org)
  • Hemidesmosomes are found in epithelial cells connecting the basal epithelial cells to the lamina lucida, which is part of the basal lamina. (wikipedia.org)
  • La integrina alfa3beta1 es la principal integrina presente en las CÉLULAS EPITELIALES, en donde desempeña un papel en el acoplamiento de la MEMBRANA BASAL y en la migración celular, y puede regular las funciones de otras integrinas. (bvsalud.org)
  • A special type of ECM is the basal membrane, which supports epitelial cells. (uncommondescent.com)
  • These neurons are hence dependent upon RG extensions to support their migration from apical to basal regions. (frontiersin.org)
  • During cortical development, RGs are present in different strategic positions: apical RGs (aRGs) have their cell bodies located in the ventricular zone with an apical process contacting the ventricle, while they also have a basal process extending radially to reach the pial surface of the cortex. (frontiersin.org)
  • This particular conformation allows aRGs to be exposed to long range and short range signaling cues, whereas basal RGs (bRGs, also known as outer RGs, oRGs) have their cell bodies located throughout the cortical wall, limiting their access to ventricular factors. (frontiersin.org)
  • Furthermore, in vitro BM invasion by the breast cancer cells MCF-7 was mediated by MT1-MMP activation of yet unidentified targets. (medscape.com)
  • In a now classic in vitro assay, cell monolayers are manually manipulated (scratched, poked, ablated with a laser) to simulate wounding. (biomedcentral.com)
  • Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
  • Geltrex matrix encourages the differentiation of human epithelial cell lines derived from mammary glands (MCF-10A) into acinar structures. (thermofisher.com)
  • The growth pattern of human epidermal cells, fibroblasts or Swiss mouse 3T3/J2 fibroblasts cultured upon the extracellular matrix (ECM) derived from small intestinal submucosa (SIS) was evaluated. (usuhs.edu)
  • Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)
  • MAPK Pathway Inhibition in Melanoma The MAPK pathway is primarily responsible for responses to growth signals within cells. (globaltechbiz.com)
  • How can we identify the proteinases used by tumor cells to cross the basement membrane (BM)? (medscape.com)
  • Finally, in Clytia , we show that damage to the basement membrane in a wound gap causes a rapid shift between the cell crawling and purse string mechanisms for wound closure. (biomedcentral.com)
  • Laminin-1 (LAM-111), which is composed of alpha1, beta1 and gamma 1 chains, is crucial for early basement membrane assembly, and embryonic implantation and development. (grantome.com)
  • The pathophysiology of DKD comprises the interaction of both genetic and environmental determinants that trigger a complex network of pathophysiological events, which leads to the damage of the glomerular filtration barrier, a highly specialized structure formed by the fenestrated endothelium, the glomerular basement membrane and the epithelial podocytes, that permits a highly selective ultrafiltration of the blood plasma. (wiley.com)
  • [8] Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest. (knowpia.com)
  • They have been much studied for their tremendous regenerative potential, but epithelial wound healing has not been characterized in detail. (biomedcentral.com)
  • ECMCintegrin connections are of particular curiosity for regenerative medication applications because if these connections can end up being specifically managed, after that, in theory, cell destiny can end up being managed. (bio2009.org)
  • Both procedures allow for special cells obtained by a patient for therapeutic purposes in regenerative medicine. (celulastroncales.org)
  • Restoration of miR-200c to aggressive cancer cells causes a decrease in migration and invasion. (hindawi.com)
  • Studies with a panel of different tumor cells and an array of protease inhibitors, however, showed that only MMP inhibitors, such as TIMP2 (a natural inhibitor) and BB94, could block invasion of the BMs by tumor cells. (medscape.com)
  • BM perforation and invasion by cancer cells was inhibited by TIMP2, the natural inhibitor that blocks both secreted and membrane-bound MMPs, but not by TIMP1, which blocks only secreted MMPs. (medscape.com)
  • These detached cancer cells are designated peritoneal-free cancer cells and the positive rate of cytological examination is associated with the depth of tumour invasion and degree of differentiation ( 8 ). (spandidos-publications.com)
  • Not only can Geltrex matrix be utilized in various assays including angiogenesis, neurite outgrowth, and tumor cell invasion assays, but also as a vehicle to supplement the tumorigenicity of injected tumor cells in nude mice. (thermofisher.com)
  • We then used classifiers and partial-least squares regression analysis to identify which metrics best predicted ECM-driven 2D migration and 3D invasion responses. (aip.org)
  • More recently, researchers developed stencils to create gaps in sheets of undamaged epithelial cells as they grow over a culture substrate, defining a "wound" of specific geometry when the stencil is removed [ 4 ]. (biomedcentral.com)
  • Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)
  • The α6 subunit binds to extracellular BP180, CD151 and laminin-322. (wikipedia.org)
  • There are 5 alpha (alpha1/LAMA1- alpha5/LAMA5), 3 beta (beta1/LAMB1- beta 3/ LAMB3), and 3 gamma (gamma1/LAMC1 - gamma3/LAMC3) chains, and at least 16 laminin isoforms can be formed by various combinations of each subunit. (grantome.com)
  • All of the mAbs to the I-domain immunoprecipitated the unassociated alphaM precursor and reacted with the alphaM subunit expressed alone on the surface of COS cells. (embl.de)
  • By contrast, four mAbs to the beta-propeller domain did not react with the unassociated alphaM precursor nor with the uncomplexed alphaM subunit expressed on COS cell surface. (embl.de)
  • A novel synthetic peptide from the B1 chain of laminin with heparin-binding and cell adhesion-promoting activities. (umn.edu)
  • by cytoskeleton adjustments, epithelial cellCcell connections, adjustments in gene reflection of mesenchymal and epithelial indicators, and injury recovery assay. (bio2009.org)
  • The expression levels of CREC family had correlations with genomic mutation and methylation, and with the infiltration levels of CD4 + T cells, macrophages, neutrophils, and dendritic cells in the microenvironment of CRC. (biomedcentral.com)
  • C1q is locally produced by immune cells such as monocytes, macrophages, and dendritic cells. (explorationpub.com)
  • This is caused by mutations in the collagen 7 gene. (wikipedia.org)
  • Mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. (cusabio.com)
  • While BRAF inhibitors predictably inhibit MEK/ERK signaling in cells harboring BRAF mutations, they paradoxically activate MEK/ERK signaling in cells harboring RAS mutations by promoting BRAF-CRAF heterodimers and homodimers. (globaltechbiz.com)
  • Hyaluronic acid is found on the inner surface of the cell membrane and is translocated out of the cell during biosynthesis. (knowpia.com)
  • 18. Monoclonal antibody and synthetic peptide inhibitors of human tumor cell migration. (nih.gov)
  • Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
  • Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
  • Pancreatic stellate cells (PSCs) are critical players in PDAC microenvironment to promote the aggressiveness and pathogenesis of the disease. (biomedcentral.com)
  • The tumor microenvironment (TME) is a complex system composed of multiple cells, such as non-cancerous fibroblasts, adipocytes, immune and vascular cells, as well as signal molecules and mediators. (encyclopedia.pub)
  • Cross-talk within the cancer microenvironment can be direct by cell-to-cell contact via adhesion molecules, electrical coupling, and passage through gap junctions, or indirect through classical paracrine signaling by cytokines, growth factors, and extracellular vesicles. (encyclopedia.pub)
  • Tumor microenvironment provides crucial signals for CSCs to maintain stem cell properties and promotes tumorigenesis. (nature.com)
  • Therefore, establishment of an appropriate cell culture system to mimic the microenvironment for CSC studies is an important issue. (nature.com)
  • This hypothesis compares the viable cancer cells to 'seeds' and the suitable microenvironment of the metastatic sites to 'soil' ( 6 ). (spandidos-publications.com)
  • We further highlight the functional implications of SA genes expressed specifically in cancer and stromal cell populations in the tumor microenvironment. (molcells.org)
  • Epithelial wounds heal either by cells crawling over the wound gap, by contraction of a super-cellular actin cable ("purse string") that surrounds the wound, or some combination of the two mechanisms. (biomedcentral.com)
  • Here we test this idea by characterizing epithelial wound healing in live medusae of Clytia hemisphaerica . (biomedcentral.com)
  • This is consistent with work in other systems showing that cells marginal to a wound choose between a super-cellular actin cable or lamellipodia formation to close wounds, and suggests a mechanism underlying this decision. (biomedcentral.com)
  • 1. Cell crawling and purse string mechanisms of epithelial wound healing likely evolved before the divergence of Cnidaria from the bilaterian lineage ~ 600mya 2. (biomedcentral.com)
  • Therefore, understanding how cells in an epithelial sheet recognize and rapidly close a wound is of tremendous importance, both as a fundamental biological question and as a factor in human health. (biomedcentral.com)
  • The purpose of this study was to evaluate whether wounding and wound healing has an effect on Ca 2+ dynamics in human embryonic stem cell (hESC)-RPEs cultured different periods of time. (biomedcentral.com)
  • This acquired knowledge about Ca 2+ dynamics of wounded hESC-RPE monolayers is important for understanding the dynamics of RPE wound healing, and could offer a reliable functionality test for RPE cells. (biomedcentral.com)
  • If left untreated, choroidal neovascularization evokes hemorrhage, retinal edema, and increased damage to retinal cells, fibrosis and permanent visual loss due to the undergoing wound healing process. (biomedcentral.com)
  • Previous studies have established that the transforming growth factor β-mothers against decapentaplegic homolog 4 (SMAD4) signaling pathway is required for effective epithelial wound healing, and a conditional deletion of SMAD4 in the epidermis causes defects in skin wound healing, which are accompanied by spontaneous skin inflammation and SCC ( 6 ). (spandidos-publications.com)
  • P1a, tetraspanin protein CD151, BPAG1e, or bullous pemphigoid antigen isoform e, and BPAG2 (also known as BP180 or type 17 collagen). (wikipedia.org)
  • Specific miRNAs have been found to be expressed in cell type-specific manner, at specific developmental stages, and in disease states including cancer [ 1 , 2 ]. (hindawi.com)
  • A second, alternative, model is provided by the peritoneal BM that consists in a bilayered structure formed by laminin and type IV collagen. (medscape.com)
  • The presence of differentiation-related keratins 10, 14 and 16, FN, laminin, collagen type VII and collagen type IV was determined by immunohistochemical methods in SIS alone and in the SIS/cell composite. (usuhs.edu)
  • Following the growth of epidermal cells and fibroblasts on the SIS substratum, immunolabeling of FN, laminin, collagen type VII and collagen type IV was observed in a cell-associated pattern. (usuhs.edu)
  • Human wild-type HeLa cell line ( ab255448 ). (abcam.com)
  • Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
  • showed that the laminin 511 matrix, one type of ECM, supported and maintained the breast CSC properties 21 . (nature.com)
  • Conditioned media from rheumatoid synovial fibroblasts contained both type I and II collagen degrading activity. (uni-bielefeld.de)
  • However, degradation of type II collagen, but not that of type I collagen, was completely inhibited by 50 mu M doxycycline, suggesting specific MMP-8 activity. (uni-bielefeld.de)
  • Transdifferentiation directly convert differentiated cells from one type to another type of differentiated cell. (celulastroncales.org)
  • 6, 8 The primitive epithelial cells that are in direct contact with the portal vein mesenchyme transform into bile duct type cells by forming a single layer and later two layers (fig 1A). (bmj.com)
  • Squamous cell carcinoma (SCC) is a histologically distinct type of cancer ( 1 ). (spandidos-publications.com)
  • Stem cells differentiate and acquire, through amazing and still poorly understood epigenetic trajectories, completely different cellular phenotypes and functions. (uncommondescent.com)
  • In this procedure it is possible to use stem cells obtained from different sources. (bvsalud.org)
  • It supports growth of a variety of cells in 2D and 3D cell culture, including primary cells, human embryonic stem cells, and human induced pluripotent stem cells. (thermofisher.com)
  • Human ESC-qualified lots of Geltrex matrix have been tested for their ability to support feeder-free culture of human embryonic stem cells (hESCs) with StemPro hESC SFM. (thermofisher.com)
  • In 1995, she moved to the University of Miami where she met her mentor Dr. Gregory Conner from the Department of Cell Biology and Anatomy, who guided her through cell and molecular biology techniques applied to airway biology. (gr.jp)
  • In addition, no information is available yet on the molecules being targeted and degraded during the perforation/migration process observed. (medscape.com)
  • Neurite outgrowth is a key event in the differentiation of neuronal cells, and is regulated by extracellular environment factors such as nerve growth factor (NGF) and cell adhesion molecules such as laminins. (grantome.com)
  • In order to be effective and integrated to the receiving area, the bone graft is required to allow a strong cell adhesion, interacting with several molecules to induce migration, differentiation, and thus the mineralization of the new bone on the graft. (bvsalud.org)
  • These cell adhesion molecules (CAM) will mediate the contact between two cells or between cells and the extracellular matrix, an essential process to the success of the implant. (bvsalud.org)
  • The main reported mechanisms involve cell adhesion molecules (CAMs) and extracellular matrix components. (bvsalud.org)
  • Classical short range factors include cell to cell signaling, adhesion molecules and mechano-transduction mechanisms (e.g. (frontiersin.org)
  • The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. (knowpia.com)
  • Since respiratory secretions are constantly cleared by ciliary action, it has been believed that the rates of synthesis and secretion of different defense molecules are the main determinants of their availability and activity on the epithelial surface. (gr.jp)
  • Regardless, the increased number of fibroblast-like cells and the deposited extracellular matrix within the alveolar walls results in distorted pulmonary architecture, vascular remodelling, decreased oxygenation, respiratory failure and death 8 . (ersjournals.com)
  • However, in vivo MMP-8 expression was observed in mononuclear fibroblast-like cells in the rheumatoid synovial membrane. (uni-bielefeld.de)
  • The corneal endothelium (CE) is a single layer of hexagonal cells that separates the corneal stroma from the aqueous humor of the anterior chamber. (intechopen.com)
  • All animals have mechanisms for healing damage to the epithelial sheets that cover the body and line internal cavities. (biomedcentral.com)
  • Both cell crawling and purse string closure of epithelial wounds are widely observed across vertebrates and invertebrates, suggesting early evolution of these mechanisms. (biomedcentral.com)
  • However, many aspects of cell-to-cell communication have yet to be clarified, and, in particular, more work is needed in regard to mechanisms of bidirectional signal transfer. (encyclopedia.pub)
  • To dissect the underlying molecular mechanisms, we established LEC co-cultures with different melanoma cells originating from primary tumors or metastases. (elifesciences.org)
  • Epithelial cells in organs such as the skin, the intestine and the cornea form sheets of closely associated cells. (biomedcentral.com)
  • But researchers also suspect that vessel cells interact with the cancer cells, helping the melanoma invade distant organs. (elifesciences.org)
  • When transplanted into mice, these cancer cells spread to and invaded the rodents' distant organs. (elifesciences.org)
  • The goal of gene therapy is to introduce new genetic material into cancer cells that selectively kills them without causing toxicity to the surrounding cells. (medscape.com)
  • Expression analysis showed that exposure of cells to DMA III resulted in a gene profile different from that in cells exposed to iAs III or MMA III , and that the iAs III -induced gene profile was closest to that in the tumorigenic HUC-1-derived 3-methylcholanthrene-induced tumorigenic cell line MC-SV-HUC T2, which was derived from SV-HUC-1 cells by methylcholanthrene treatment. (nih.gov)
  • The heterogeneity of SA gene expression in cancer cells not only promotes cancer stemness but also protects these cells from chemotherapy. (molcells.org)
  • 19. Altered extracellular matrices influence cellular processes and nuclear matrix organizations of overlying human bladder urothelial cells. (nih.gov)
  • Can tumor cells invade these cellular constructs? (medscape.com)
  • PC12 cells grown on ECM Gel will show extensive neurite outgrowth containing complex 3D neural networks that are observable with an inverted microscope (Figure 2. (sigmaaldrich.com)
  • Neurite outgrowth of PC12 rat neuronal cells using ECM Gel Matrix. (sigmaaldrich.com)
  • However, the role of GM1 in laminin-1-induced neurite outgrowth is not clear. (grantome.com)
  • Additionally, we observe that restoration of miR-200c to ovarian cancer cells causes a decrease in adhesion to laminin. (hindawi.com)
  • For example, ovarian cancer cells embedded within gelatin-methacrylamide hydrogels displayed a higher drug resistance 17 . (nature.com)
  • ECM constituents mainly derive from the tumor cells themselves but also, to a large degree, from cancer-associated fibroblasts (CAF). (encyclopedia.pub)
  • Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. (celulastroncales.org)
  • Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. (sigmaaldrich.com)
  • But if you look at the origin of this membrane, it is derived from the epiblast which is the outer cell mass and the fetus is derived from the inner cell mass. (cybersight.org.cn)
  • The amniotic membrane is about 0.02-0.4mm thick and it's got an epithelial layer as we discussed. (cybersight.org.cn)
  • The CSDH outer membrane, which contains inflammatory cells, plays an important role in CSDH development. (mdpi.com)
  • This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)
  • That is specially true for important cell systems, first of all the immune system. (uncommondescent.com)
  • In addition to its involvement in defence against a range of pathogens and clearance of apoptotic and necrotic cells, C1q has also been implicated in immune and non-immune homeostasis. (explorationpub.com)
  • By virtue of the local production of C1q by immune and non-immune cells, its role in pregnancy and its complications has also been examined recently [ 4 , 5 ]. (explorationpub.com)
  • CD151, a protein of the tetraspanin superfamily, resides on the cell surface of keratinocytes and vascular endothelium. (wikipedia.org)
  • Lot-to-lot differences in BME protein concentration can cause significant variability in cell culture experiments. (thermofisher.com)
  • HN - 2024 BX - Aflatoxicosis BX - Aflatoxin Toxicity MH - AIRE Protein UI - D000096927 MN - D12.776.930.123 MS - A transcriptional regulator primarily expressed in medullary thymic epithelial cells. (nih.gov)