• Secretory epithelial cells display lactogenic differentiation which is characterized by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
  • To explore whether the downregulation of AP-2alpha and AP-2gamma is of functional significance for lactogenic differentiation, we analyzed the expression of the AP-2 family members during the lactogenic differentiation of HC11 mammary epithelial cells in vitro. (biomedcentral.com)
  • Differentiation of HC11 cells was induced following established protocols by applying the lactogenic hormones prolactin, dexamethasone and insulin. (biomedcentral.com)
  • Using RT-PCR we could not detect a downregulation of any of these genes during the lactogenic differentiation of HC11 cells in vitro. (biomedcentral.com)
  • The extent of lactogenic differentiation of HC11 cells in vitro is limited compared to mammary epithelium undergoing secretory differentiation in vivo. (biomedcentral.com)
  • Downregulation of AP-2 transcription factor genes is not required for lactogenic differentiation of HC11 cells but may functionally be involved in aspects of lactogenic differentiation in vivo that are not reflected by the HC11 system. (biomedcentral.com)
  • The lactogenic differentiation of the secretory epithelial cells is characterized by the synthesis of lactose and milk fat and by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
  • HC11 cells can be induced to undergo lactogenic differentiation by applying a mixture of the lactogenic hormones dexamethasone, insulin and prolactin (a mixture termed DIP in the following sections). (biomedcentral.com)
  • In this report we analyzed AP-2 transcription factor expression in HC11 cells subjected to lactogenic differentiation conditions. (biomedcentral.com)
  • For induction of differentiation, cells were grown to confluency and then kept in medium without EGF for 48 hours to induce competence. (biomedcentral.com)
  • Proportions of CD68+ cells and CD3+ cells that coexpressed MO/MA differentiation factors (CD163, CCR1, CXCR8, VCAM1, and phosphorylated cytosolic phospholipase A 2 [pcPLA 2 ]), which had demonstrated expression in EOC peritoneal samples, were determined by multicolor immunofluorescence. (biomedcentral.com)
  • Translation factors control the translation of all proteins including those vital for cell growth and differentiation as well as those involved in cellular response to chemical toxicity. (cdc.gov)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • Semaphorin 4D (Sema4D) belongs to the semaphorin family, which binds to receptors such as Pleixn-B1 (transmembrane structure receptor), CD72 (cluster of differentiation antigen 72, mainly expresses on lymphocyte cells), then activates downstream signaling molecules. (jcancer.org)
  • Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
  • These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
  • T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
  • A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
  • While abnormal keratin filament structure and function can explain hypohidrosis and epithelial differentiation abnormalities, the absence of dermatoglyphics is not well understood. (medscape.com)
  • Epsin Family Member 3 and Ribosome-Related Genes Are Associated with Late Metastasis in Estrogen Receptor-Positive Breast Cancer and Long-Term Survival in Non-Small Cell Lung Cancer Using a Genome-Wide Identification and Validation Strategy. (nih.gov)
  • Differentiating HC11 cells displayed lower expression levels of milk protein genes than mammary glands of mid-pregnant or lactating mice. (biomedcentral.com)
  • We previously showed that tumor-free peritoneum of patients with epithelial ovarian cancer (EOC) exhibited enhanced expression of several inflammatory response genes compared to peritoneum of benign disease. (biomedcentral.com)
  • Here, we examined peritoneal inflammatory cell patterns to determine their concordance with selected enhanced genes. (biomedcentral.com)
  • The strategy was developed to compare the variations in complex genomes as well as to obtain clones of those differentiating genes (Lisitsyn and Wigler 1993 Before the availability of the genome sequences RDA was applied to reveal genes distinctively or predominantly present in just one subspecies (Gorkiewicz ssp. (researchensemble.com)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • The first project focuses on E2F1, a regulator of genes important for cell cycle progression and apoptosis. (mdanderson.org)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Amongst the intestinal Wnt target genes (13), we found the Gpr49/Lgr5 gene to be unique in that it marks small cycling cells at crypt bottoms. (hubrecht.eu)
  • Two other Wnt target genes, RNF43 and ZNRF3, encode stem cell-specific E3 ligases that downregulate Wnt receptors in a negative feedback loop (35). (hubrecht.eu)
  • K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • 1997) and epithelial cell monolayer in vitro (Espinosa- Cantellano et al . (scialert.net)
  • In addition, miR‑205 overexpression reduced and miR‑205 depletion enhanced PC cell proliferation and migration in vitro. (spandidos-publications.com)
  • Notably, overexpression of RUNX2 partially reversed the inhibitory effect of miR‑205 on PC cell proliferation and migration in vitro. (spandidos-publications.com)
  • To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • A bioethics board at UCLA recently approved a clinical trial of cells Lanza has produced from human embryonic stem cells-obtained from donated in vitro fertilization embryos-to treat blindness. (robertlanza.com)
  • Lgr5 resides in Wnt receptor complexes and mediates signaling of the Wnt-agonistic R-spondins (31), explaining the unique dependence of Lgr5 stem cells on secreted R-spondins in vivo and in vitro. (hubrecht.eu)
  • Importantly, stimulation of T cells by Cd44v7- deficient macrophages induced upregulation of Foxp3 in vitro , while cotransfer of Cd44 WT macrophages into Cd44v7- deficient mice reduced Foxp3 + T-cell counts and caused colitis. (nature.com)
  • The adherence molecule Vascular Cell Adhesion Molecule-1 (VCAM1) was expressed on CD31+ endothelial cells and on a proportion of CD68+ MO/MA but rarely on CD3+ cells. (biomedcentral.com)
  • Mismatch repair proteins correct King Khalid University Hospital, King mal nuclear staining in lymphocytes the insertion and deletion mutations Saud University, Riyadh, Saudi Arabia and normal epithelial or stromal cells that occur when DNA is copied be- between 2007 and 2009 were retrieved was required serving as internal con- fore cell division. (who.int)
  • VHL disease encompasses various types of tumors, including retinal or central nervous system hemangioblastoma (RH or CNSH), clear cell renal cell carcinoma (RCC), pheochromocytoma (PHE), multiple pancreatic cysts or tumors (PCT), epididymal or broad ligament cystadenomas and endolymphatic sac tumor. (biomedcentral.com)
  • It is perhaps a paradox that the peritoneum which is organized to protect the integrity of intraabdominal organs by facilitating infiltration of inflammatory cells to sites of injury and infection, might also serve to facilitate the promotion of tumor growth and spread. (biomedcentral.com)
  • As EOC advances and penetrates the capsular layer of the ovary, it also carries the potential to expose the peritoneal surface to tumor-cell secreted products. (biomedcentral.com)
  • After sensitization with cytotoxic drugs, scFv62-TRAIL induced apoptosis only in K V 10.1-positive cancer cells, but not in non-tumor cells, nor in tumor cells lacking K V 10.1 expression. (biomedcentral.com)
  • We could design a strategy that selectively kills tumor cells based on a K V 10.1-specific antibody. (biomedcentral.com)
  • This study clearly indicates that the epithelial tumor cells synthesize different collagenases and TIMP-1. (uni-bielefeld.de)
  • No tumor-specific expression levels of protein kinase C isoenzymes and of c-fos in human breast cancer cell cultures. (shengsci.com)
  • Twenty-five of these cell cultures stemmed from normal and 21 from tumor tissues. (shengsci.com)
  • The present data provide evidence that Snail promotes tumor cell motility and angiogenesis which is mainly mediated through the regulation of RhoA activity. (spandidos-publications.com)
  • Tumor cell motility is the hallmark of invasion and an essential step in metastasis. (spandidos-publications.com)
  • Cancer genetics, DNA damage response, apoptosis, cell cycle My current research is focused on two transcription factors that respond to DNA damage and play important roles in regulating tumor development. (mdanderson.org)
  • For metastasis from a primary tumor site, cancer cells must lose cell-cell adhesion and acquire motility to invade adjacent cell layers [ 6 ]. (biomedcentral.com)
  • Translation initiation factor 5A2 (TIF5A2) and translation elongation factor 1delta (TEF1 delta) also exhibited significant overexpression (10- and 5-fold overexpression, respectively) in the tumor cells compared with the corresponding control cells. (cdc.gov)
  • Furthermore, studies have shown HIF-mediated Sema4D induction in the generation of the pro-angiogenic phenotype in oral squamous cell carcinoma (OSCC) and have determined its biological significance for tumor growth and vascularity ( 10 ). (jcancer.org)
  • Hypoxia is the most common characteristic in the microenvironment of solid tumors because tumor progression and metastasis depend on the ability of cancer cells to initiate angiogenesis and ensure the delivery of oxygen and nutrients ( 11 , 12 ). (jcancer.org)
  • Overexpression of Epsin 3 enhances migration and invasion of glioma cells by inducing epithelial‑mesenchymal transition. (nih.gov)
  • Further analysis of independent cohorts of patients with renal carcinoma revealed specific HIF2α gene expression signatures in the context of VHL Ser65Pro or Ser65Trp mutation, showing high correlations with hypoxia and epithelial-mesenchymal transition signaling activities and strong associations with poor prognosis. (biomedcentral.com)
  • One important insight came from the discovery that the increased motility and invasiveness of cancer cells is reminiscent of the epithelial-mesenchymal transition (EMT) that occurs during embryonic development ( 2 ). (spandidos-publications.com)
  • This process shares many similarities with epithelial-mesenchymal transition (EMT), which has been proposed as one of critical mechanisms for the acquisition of metastatic capacity by epithelial cancer cells [ 7 ]. (biomedcentral.com)
  • The peritoneum and its extension, the intestinal serosa, include a vast surface area for transit of inflammatory cells into the abdominal cavity. (biomedcentral.com)
  • The Wnt target gene encoding the transcription factor Achaete scute-like 2 controls intestinal stem cell state (26). (hubrecht.eu)
  • Daughters of the small intestinal stem cells, the Paneth cells, serve as crypt niche cells by providing Wnt, Notch and EGF signals (33). (hubrecht.eu)
  • Here, macrophages and T cells accumulate in the lamina propria of the intestinal tract, activate each other, destroy the regular architecture of the gut wall, and disturb the intestinal function. (nature.com)
  • Several molecules expressed by macrophages and T cells including interleukin-12 (IL-12), interferon-γ, IL-10, and Foxp3 are critical for the intestinal balance between immune attack and tolerance. (nature.com)
  • RhoA has been implicated in the formation of stress fibers and cell adhesion in fibroblasts. (spandidos-publications.com)
  • Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • In this study, we explored the contribution of c-Jun N-terminal kinases (JNK) signaling to the mesenchymal phenotypes and the aberrant motile capacity of MDR cells utilizing a well characterized MDR cell line KB/VCR, which is established from KB human epidermoid carcinoma cells by vincristine (VCR), and its parental cell line KB. (biomedcentral.com)
  • Taking advantage of experimental strategies including pharmacological tool and gene knockdown, we showed here that interference with JNK signaling pathway by targeting JNK1/2 or c-Jun reversed the mesenchymal properties of KB/VCR cells to epithelial phenotypes and suppressed the motile capacity of KB/VCR cells, such as migration and invasion. (biomedcentral.com)
  • These observations support a critical role of JNK signaling in maintaining the mesenchymal properties of KB/VCR cells. (biomedcentral.com)
  • Furthermore, we observed that JNK signaling may control the expression of both snail and twist1 in KB/VCR cells, indicating that both snail and twist1 are involved in controlling the mesenchymal characteristics of KB/VCR cells by JNK signaling. (biomedcentral.com)
  • and JNK signaling may maintain the mesenchymal characteristics of KB/VCR cells potentially through snail and twist1. (biomedcentral.com)
  • EMT, a highly conserved cellular program in many important phases of embryonic development, is a biological process through which epithelial cells lose their epithelial cobblestone phenotype and acquire fibroblastic and mesenchymal-like phenotypes. (biomedcentral.com)
  • The hallmarks of EMT are characterized by loss of epithelial adhesion molecule E-cadherin and gain of mesenchymal markers such as N-cadherin, vimentin, et al . (biomedcentral.com)
  • Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. (bio-rad-antibodies.com)
  • Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes. (bio-rad-antibodies.com)
  • The mucosa in ulcerative colitis (UC) is replete with antibody producing plasma B cells and polymorphonuclear leucocytes (PMN). (bmj.com)
  • This combination of effector cells requires a crosslinking antigen to evoke an antibody driven PMN inflammatory response via their Fc receptors. (bmj.com)
  • This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.co.jp)
  • Note: When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed. (bdbiosciences.com)
  • The K V 10.1-specific scFv62 antibody -TRAIL fusion protein was expressed in CHO-K1 cells, purified by chromatography and tested for biological activity. (biomedcentral.com)
  • In PAE cells, soluble Sema4D promoted cell migration and developed a "tubulogenic" phenotype ( 4 ). (jcancer.org)
  • Cloning and initial characterization of human epsin 3, a novel matrix-induced keratinocyte specific transcript. (nih.gov)
  • Cloning and characterization of SREHP gene from an Iranian E. histolytica isolate was also studied (Rasti et al . (scialert.net)
  • Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species. (rusmedserv.com)
  • Sakamaki K, Tomonaga M, Tsukui K, Nagata S. Molecular cloning and characterization of a chromosomal gene for human eosinophil peroxidase. (rusmedserv.com)
  • Isolation and characterization of a cDNA clone for porcine thyroid peroxidase. (rusmedserv.com)
  • Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • Epithelial ovarian cancer (EOC) results in 5 year survival rates of only 25-30% for patients with stage III and IV disease [ 1 ], contrasting with the 90% survival rates of patients with stage I disease, where notably peritoneal and serosal disease is absent. (biomedcentral.com)
  • By contrast, miR-205 expression has been identified to be significantly increased in several human cancer types, including ovarian cancer, endometrial cancer and laryngeal squamous cell carcinoma, in which it was identified to function as an oncoprotein ( 10 , 11 , 13 ). (spandidos-publications.com)
  • 2007). These proteins are important for the regulation of intracellular pH (pH i ) and play crucial roles in the epithelial absorption of HCO 3 - (e.g., in the renal proximal tubule) and secretion of HCO 3 - (e.g., in the pancreatic duct). (tcdb.org)
  • Research on vitamin D regulation of thymocyte selection, Th1 and Th17 cells, T-cell programed cell death, and T-regulatory (Treg) cells is summarized and integrated into model mechanisms. (frontiersin.org)
  • [ 10 , 11 ] In an animal model of Hartnup disorder, mice lacking SLC6A19 (B 0 AT1) transporter general neutral aminoaciduria were observed, as well as the decreased body weight, demonstrating the essential role of epithelial amino acid uptake in optimal growth and bodyweight regulation. (medscape.com)
  • In addition, these two binding sites play different roles in the regulation of Sema4D expression in HUVEC and Caco-2 cells. (jcancer.org)
  • Voted YES on expanding research to more embryonic stem cell lines. (ontheissues.org)
  • Voted YES on allowing human embryonic stem cell research. (ontheissues.org)
  • To provide for human embryonic stem cell research. (ontheissues.org)
  • There are over 3,000 Americans who die every day from diseases that could be treated with embryonic stem-cell therapies. (robertlanza.com)
  • After President Bush banned the use of federal money for most embryonic-stem-cell research in 2001, it was left to private companies (or academic labs using private money) to carry the ball. (robertlanza.com)
  • The recent findings of the pluripotent epithelial cells demonstrates how quickly the world has changed. (ontheissues.org)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • In this process, epithelial cells acquire fibroblast-like properties such as the functional loss of E-cadherin. (spandidos-publications.com)
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • In particular, in recent years, the laboratory has focused on developing an ex vivo cellular model for the functional analysis of the CFTR protein, directly from patient's nasal epithelial cells. (unina.it)
  • We set up a novel ex vivo approach for the functional analysis of the CFTR protein, directly from patient's nasal epithelial cells. (unina.it)
  • This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. (cdc.gov)
  • Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
  • Cloning and expression of the mouse SLC6A19 gene demonstrated that this transporter has all the properties of the amino acid transport system B 0 AT1. (medscape.com)
  • Consistent findings include a bandlike subepithelial mononuclear infiltrate consisting of T cells and histiocytes, increased numbers of intraepithelial T cells, and degenerating basal keratinocytes that form colloid (Civatte, hyaline, cytoid) bodies, which appear as homogenous eosinophilic globules. (medscape.com)
  • Degeneration of the basal keratinocytes and disruption of the anchoring elements of the epithelial basement membrane and basal keratinocytes (eg, hemidesmosomes, filaments, fibrils) weakens the epithelial-connective tissue interface. (medscape.com)
  • The ultrastructure of the colloid bodies suggests that they are apoptotic keratinocytes, and recent studies using the end-labeling method revealed DNA fragmentation in these cells. (medscape.com)
  • In contrast to CD44s, the CD44 isoforms containing variable regions are expressed only in distinct epithelial cell types (e.g., keratinocytes), activated immune cells, and several types of cancers. (nature.com)
  • We propose the outline of such a mechanism based on following aspects: (1) The glomerulus is a complex structure, the stability of which depends on the cooperative function of the basement membrane, mesangial cells and podocytes, counteracting the distending forces originating from the high glomerular hydrostatic pressures. (scienceopen.com)
  • Among the translation factors studied, the expression level of translation elongation factor 1A2 (TEF1A2) exhibited the highest alteration (approximately 2000-fold overexpression) in the cancer cell lines compared with the corresponding controls. (cdc.gov)
  • Meanwhile, a Texas hospital is testing whether stem cells from a patient's bone marrow will improve the effectiveness of cardiac bypass surgery. (robertlanza.com)
  • Multiple sclerosis and T1D have distinct target organs, genetic risk factors, onset ages, and female to male ratios, but target organ-specific T cells as initiators unite these diseases. (frontiersin.org)
  • The malignant clone secretes increased amounts of distinct collagenases compared to the non-tumorigenic cell line, thereby verifying a correlation between biological behaviour and the amount of collagenases. (uni-bielefeld.de)
  • their evolution from distinct cell lineages. (lu.se)
  • In addition, the results revealed that miR-205-induced downregulation of RUNX2 was associated with the inhibition of PC cell proliferation and migration. (spandidos-publications.com)
  • Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
  • Proliferation of KRT14 -expressing basal cells leads to development of dermatoglyphics during the first trimester of gestation. (medscape.com)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Role of intracellular trafficking of thyroperoxidase and involvement generated at the apical surface of thyroid cells in covalent heme binding. (rusmedserv.com)
  • The disease is due to altered salt transport across the apical border of epithelial cells, namely pancreas, lung, intestine, male genital tract and liver cells. (unina.it)
  • Cadmium -induced cell transformation and tumorigenesis were found, at least in part, to be mediated through overexpression of TIF3 and TEF-1delta. (cdc.gov)
  • A recombinant form of PDC is cloned and used to detect the presence of AMA in serum via EIA. (medscape.com)
  • J Cell Biol (2002) 157 (1): 161-172. (rupress.org)
  • Moreover, five additional members, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F, were discovered within a short period of time since 2000 to 2002 [ 3 - 9 ]. (hindawi.com)
  • 2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
  • Prostate cancer cells, either positive or negative for K V 10.1 were treated with the purified construct. (biomedcentral.com)
  • In co-cultures with K V 10.1-positive cancer cells the fusion protein also induced apoptosis in bystander K V 10.1-negative cancer cells, while normal prostate epithelial cells were not affected when present as bystander. (biomedcentral.com)
  • Similarly, expression levels of the translation factors were investigated in human prostate epithelial cells, RWPE1, exposed to a cytotoxic concentration of cadmium chloride. (cdc.gov)
  • Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45-65 nm within the entire cell volume at low light intensities (W-kW cm −2 ). (nature.com)
  • Finally, unanswered questions relating to vitamin D mechanisms in CD4 + T cells are highlighted to promote further research that may lead to a deeper understanding of autoimmune disease molecular etiology. (frontiersin.org)
  • During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
  • Molecular cloning of cDNAs encoding bovine and human lactoperoxidase. (rusmedserv.com)
  • In this regard, elucidating the molecular mechanisms underlying aberrant metastatic capacity of MDR cells is quite important, as it may provide new targets for improving the efficiency of chemotherapy. (biomedcentral.com)
  • The Organoid group, previously Clevers group, studies the molecular mechanisms of tissue development and cancer of various organs using organoids made from adult Lgr5 stem cells. (hubrecht.eu)
  • In APC-deficient colon carcinoma cells, we demonstrated that ß-catenin accumulates and is constitutively complexed with the TCF family member TCF4, providing a molecular explanation for the initiation of colon cancer (5). (hubrecht.eu)
  • Semaphorin 4D (Sema4D) has been proven to be one of the hypoxia effectors regulated by hypoxia inducible factor (HIF-1) in multiple cells, and play a role in angiogenesis like VEGF. (jcancer.org)
  • This is especially relevant in dim structures with moderately low signal to noise ratio (SNR), such as in cells exhibiting endogenous levels of rsFP fusion expression and in 3D samples where out-of-focus background dominates. (nature.com)
  • A number of studies have demonstrated that altered expression of miRs serves critical roles in human cancers by directly regulating cell behaviors ( 5 - 7 ). (spandidos-publications.com)
  • Zhang et al ( 9 ) revealed that expression of miR-205 was significantly decreased in radioresistant subpopulations of breast cancer cells and loss of miR-205 expression was associated with poor distant relapse-free survival in patients with breast cancer. (spandidos-publications.com)
  • By employing the real time PCR technique, we have investigated the expression levels of various translation factors in human cancer cell lines and their corresponding controls. (cdc.gov)
  • IHC-P: Human lung and HEK-293T (human embryonic kidney epithelial cell) transfected with a SCGB1A1 expression vector containing a his tag tissues. (abcam.com)
  • ICC/IF: HEK-293T cells transfected with a human Uteroglobin expression vector containing a myc-his tag. (abcam.com)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • was shown to encode a conjugation‐related type IVa macromolecular secretion system (T4SS) (for system classifications observe Christie and Vogel 2000 Mutational analyses confirmed the secretion machinery is definitely involved in the ability of ssp. (researchensemble.com)
  • We investigated cells and conditioned media of the three human keratinocyte cell lines HaCaT (non-tumorigenic), A5 (benign, tumorigenic) and II-4RT (malignant, tumorigenic) with regard to production and secretion of the collagenases-1 to -3 (MMP-1, MMP-8 and MMP-13) and TIMP-1 using semi-nested RT-PCR, Western blots, ELISA, immunocytochemistry and casein zymography. (uni-bielefeld.de)
  • represents a bovine clone (vehicle Bergen varieties which show considerable genetic variance (e.g. subspecies are expected to bring quick advances. (researchensemble.com)
  • VWR provides the cell culture community with access to the most reliable supply of exceptional quality Fetal Bovine Serum: VWR Life Science Seradigm. (vwr.com)
  • Moreover, of the 2000 identified gene variants, the disease-causing effect remains unproven for most of these variants, especially for missense ones. (unina.it)
  • There were three nucleotide variants (T471C/A600G/C862T) frequently detected in cancer cell lines. (jcancer.org)
  • The CF Transmembrane Conductance Regulator (CFTR) gene, which causes CF, was cloned in 1989. (unina.it)
  • However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. (bdbiosciences.com)
  • 2000. Documentation of the threshold limit values and biological indices. (cdc.gov)
  • Description: A sandwich ELISA kit for detection of Nischarin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (lotuskringpoeldijk.nl)
  • Since 2 years ago, the last Stem Cell bill, public support has surged for stem cells. (ontheissues.org)
  • Instead, we should foster all of these methods, and we should adequately fund and have ethical oversight over all ethical stem cell research. (ontheissues.org)
  • As chief medical officer of Advanced Cell Technology, a leading stem-cell company, he has no doubt that adult stem cells will fall woefully short of the promise of embryonic ones. (robertlanza.com)
  • His single-minded determination has made him a punching bag for U.S. senators, the Catholic Church, and his fellow stem-cell scientists, to name but a few who have attacked his research. (robertlanza.com)
  • We were the first to link Wnt signaling with adult stem cell biology, when we showed that TCF4 gene disruption leads to the abolition of crypts of the small intestine (8), and that TCF1 gene knockout severely disables the stem cell compartment of the thymus (2). (hubrecht.eu)
  • These cells represent the epithelial stem cells of the small intestine and colon (23), the hair follicle (24), the stomach (28) and many other tissue stem cell types. (hubrecht.eu)
  • These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (32, 47, 53). (hubrecht.eu)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues. (nature.com)
  • Observing the interplay of organelles and macromolecular complexes inside living cells and tissues calls for the continuous development of minimally invasive optical systems performing at high spatio-temporal resolution. (nature.com)
  • A nanoscope able to record robust raw data rapidly and with sub-100 nm spatial resolution across the entire 3D space of cells and tissues is still missing. (nature.com)
  • In mammals, physiological Wnt signaling is intimately involved with the biology of adult stem cells and self-renewing tissues (18,19). (hubrecht.eu)
  • Tissues and Cells. (lu.se)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • EPN3 plays oncogenic role in non-small cell lung cancer by activating the JAK1/2-STAT3 pathway. (nih.gov)
  • Alkenyl group is responsible for the disruption of microtubule network formation in human colon cancer cell line HT-29 cells. (shengsci.com)
  • Epithelial cells derived from 46 human breast tissue samples of patients suffering from breast cancer have been cultivated. (shengsci.com)
  • In addition to possess cross drug resistance characteristic, emerging evidences have shown that multiple-drug resistance (MDR) cancer cells exhibit aberrant metastatic capacity when compared to parental cells. (biomedcentral.com)
  • The Tcf4-driven target gene program in colorectal cancer cells is the malignant counterpart of a physiological gene program in selfrenewing crypts (13, 14). (hubrecht.eu)
  • Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)
  • The site variation rates of T471C/A600G/C862T were 72.7%, 18.2%, and 72.7% in cancer cells respectively. (jcancer.org)
  • Loss of sensitivity to p21 cell cycle control may be associated with prostatic cancer behavior. (scirp.org)
  • arations verified the presence of cancer cells in all samples. (lu.se)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • Unlike the normal mucosa, the UC mucosa contains large numbers of IgG plasma cells, reminiscent of a typical peripheral immune response towards an invading pathogen, leading to local deposition of IgG on epithelial cells. (bmj.com)
  • Avantor® can help equip your life sciences lab with the products, equipment, and supplies you need - whether you work in cell biology, genomics, proteomics, or other fields. (vwr.com)
  • Lgr6 marks multipotent skin stem cells (29). (hubrecht.eu)
  • Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
  • TRAIL is normally present as a membrane protein (memTRAIL) on immune effector cells, like natural killer cells. (biomedcentral.com)
  • Biofilm expres- sion by the outbreak clone was greater than that of spo- infection (8,11). (cdc.gov)
  • We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
  • Exposure of RWPE1 cells to a cytotoxic concentration of cadmium chloride also resulted in overexpression of several translation factors. (cdc.gov)
  • Furthermore, because of the antigenic and genetic diversity of 'Norwalk-like viruses'* (NLVs) and the inability to cultivate these viruses in cell lines, developing assays to detect the full spectrum of NLVs associated with outbreaks of gastroenteritis was not possible. (cdc.gov)
  • Sections were first stained by indirect immunoperoxidase and numbers of monocytes/macrophages (MO/MA), T cells, B cells, and NK cells counted. (biomedcentral.com)
  • Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. (biolegend.com)
  • Biopsy may be required to exclude malignancy or to differentiate between oral lichen planus and other white or chronic ulcerative oral lesions, including reactive keratoses, chronic hyperplastic candidosis, epithelial dysplasia, discoid lupus erythematosus, gastrointestinal disease (including oral Crohn disease), and anemic states. (medscape.com)