Epithelial cell line teratocarcinoma. Medical search. Frequent questions

EBs are differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. (wikipedia.org)
Similar to ESCs cultured in monolayer formats, ESCs within embryoid bodies undergo differentiation and cell specification along the three germ lineages - endoderm, ectoderm, and mesoderm - which comprise all somatic cell types. (wikipedia.org)
Therefore, the three-dimensional structure, including the establishment of complex cell adhesions and paracrine signaling within the EB microenvironment, enables differentiation and morphogenesis which yields microtissues that are similar to native tissue structures. (wikipedia.org)
When cultured as single cells in the absence of anti-differentiation factors, ESCs spontaneously aggregate to form EBs. (wikipedia.org)
EB differentiation begins with the specification of the exterior cells toward the primitive endoderm phenotype. (wikipedia.org)
It is widely used in biomedical research, mainly for studying developmental biology, stem cell biology, cell differentiation, and drug screening. (cytion.com)
As P19 cells have differentiation ability, they can be useful in investigating complex biological processes such as tissue formation and early embryonic development. (cytion.com)
Thus, P19 cells can be useful in studying cell differentiation and developmental processes. (cytion.com)
These cells are the original precursors of various cells in a tumor, whereas other intratumoral cells are limited in terms of their potential for proliferation and pluripotent differentiation. (biomedcentral.com)
In addition, normal adult stem cells (ASCs) self-renew, proliferate infinitely, have a multidirectional differentiation potential, similar to that of tumor cells, and can transform more easily into tumor stem cells than non-stem cells [ 20 , 21 ]. (biomedcentral.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Wnt ligands are secreted glycoproteins that function in intracellular signaling pathways to regulate a variety of developmental processes including cell growth, cell differentiation, cell polarity, and apoptosis. (biomedcentral.com)
Role of Otx2 in Embryonic Stem Cells (ESCs): Otx2 is an intrinsic determinant required to maintain the ESC metastable state by antagonizing ground state pluripotency and promoting commitment to differentiation. (cnr.it)
discovered the differentiation of alveolar type II epithelial cells in ECM extract [10] . (danabiosci.com)
The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
CFC1 inhibited Activin A-induced NB cell differentiation and Smad2 phosphorylation in NB cell lines, indicating its involvement in tumorigenesis related to EGF-CFC co-receptor family molecule pathways. (oncotarget.com)
Accumulating evidence has shown that cancer stem cells (CSCs) are a small subpopulation of tumor cells that exhibit the properties of self-renewal, differentiation into various tumor cell types, tumorigenicity, and chemo/irradiation resistance [ 1 ]. (oncotarget.com)

We speculate that precancerous stem cells (pCSCs) arise during the evolution of carcinomas based on long-term experimental findings, published literature, and the cancer stem cell (CSC) theory, wherein pCSCs exist in precancerous lesions and have characteristics of both CSCs and normal stem cells. (biomedcentral.com)
Carcinomas, malignancies of epithelial tissue, account for 80 to 90 % of all cancer cases since epithelial tissues are most abundantly found in the body from being present in the skin to the covering and lining of organs and internal passageways, such as the gastrointestinal tract. (solutionpharmacy.in)
Cripto-1 plays an important oncogenic role during tumorigenesis and is overexpressed in a wide range of epithelial carcinomas, yet little is known about Cripto-1 in nasopharyngeal carcinoma (NPC). (biomedcentral.com)
Six genes carrying DNVs were associated with human developmental disorders involving epithelial, connective or bone morphologies ( PXDN, RTEL1, ANKRD11, MAP2K1, CYLD, ACAN) and four linked with cholangio- and hepatocellular carcinomas (PIK3CA, TLN1 CYLD, MAP2K1) . (biomedcentral.com)

NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG1, κ Isotype Control (Cat. (bdbiosciences.com)
Three cisplatin resistant sublines (NTERA-2-R, NCCIT-R, 2102EP-R) showing 2.7-11.3-fold increase in drug resistance after intermittent exposure to increasing doses of cisplatin were compared to their parental counterparts, three well established relatively cisplatin sensitive germ cell tumor cell lines (NTERA-2, NCCIT, 2102EP). (biomedcentral.com)
The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)

Supporting differences in Wnt signaling, gain of function of Wnt-1 in C57mg mouse mammary epithelial cells leads to their morphological transformation while loss of function of Wnt-5a leads to the same transformation. (biomedcentral.com)
To test the hypothesis that a specific cell line can respond to distinct Wnts with different patterns of gene expression, we over-expressed Wnt-5a and Rfz-2 in C57mg mammary epithelial cells and compared this cell line to C57mg cells over-expressing Wnt-1. (biomedcentral.com)
the first being a class that transforms C57mg mouse mammary epithelial cells and also promotes duplication of the dorso-ventral axis when over-expressed in Xenopus embryos. (biomedcentral.com)
In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)

Errors arising in stem and progenitor cells have a much greater influence on the tissue in which they are found than errors arising in postmitotic differentiated cells. (biomedcentral.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
For example, normal stroma, the connective material that supports the cells of a tissue, appears to inhibit cancer growth. (the-scientist.com)
Degradation of the extracellular matrix allows tumor cells to detach from the primary tumor mass, invade local tissue, intravasate, extravasate and build new metastatic formations [ 1 ]. (biomedcentral.com)
When cells in some area of the body duplicate without control, the excess of tissue develops called tumor or neoplasm. (solutionpharmacy.in)
Other examples include Chondrosarcoma (of the cartilage), Leiomyosarcoma (smooth muscles), Rhabdomyosarcoma (skeletal muscles), Mesothelial sarcoma or mesothelioma (membranous lining of body cavities), Fibrosarcoma (fibrous tissue), Angiosarcoma or hemangioendothelioma (blood vessels), Liposarcoma (adipose or fatty tissue), Glioma or astrocytoma (the neurogenic connective tissue found in the brain), Myxosarcoma (primitive embryonic connective tissue) and Mesenchymous or mixed mesodermal tumor (mixed connective tissue types). (solutionpharmacy.in)
Oddly enough, the serum MCP-1 Metoclopramide HCl level elevated and peaked at six h after c-lipo pretreatment quickly, suggesting which the MCP-1 made by c-lipo-phagocytized Compact disc68+ Kupffer cells Metoclopramide HCl may recruit Compact disc11b+ macrophages in the periphery and bone tissue marrow. (enmd-2076.com)

Embryoid bodies (EBs) are three-dimensional aggregates of pluripotent stem cells. (wikipedia.org)
Additionally, EBs can be formed from embryonic stem cells derived through alternative techniques, including somatic cell nuclear transfer or the reprogramming of somatic cells to yield induced pluripotent stem cells (iPS). (wikipedia.org)
Pluripotent stem cells (PSCs) were first isolated and established from mouse embryos in 1981 [7] [8] . (danabiosci.com)
Human-induced pluripotent stem cells (iPSCs) were successfully produced by reprogramming mouse and human fibroblasts in 2006, which has had a major impact on stem cell and organoid research [12] . (danabiosci.com)
Organoids can be generated from two types of stem cells: pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), and organ-restricted adult stem cells (AdSCs). (danabiosci.com)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)

The growth of neoplastic cells exceeds and is not coordinated with that of the normal tissues around it. (solutionpharmacy.in)
laid the foundations of brain organoids by demonstrating the self-organization of human brain iPSCs into neural cells that formed polarized cortical tissues [13] . (danabiosci.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

P19 is a murine embryonal carcinoma cell line. (cytion.com)
Forkhead box P3-peptide inhibitor P60 has been shown to specifically inhibit regulatory T-cell function in murine models. (bvsalud.org)
Aim of this study was to investigate whether P60 can improve the immune response induced by vaccination with adenovirus-transduced dendritic cells expressing alpha-fetoprotein in subcutaneous and orthotopic murine models for hepatocellular carcinoma. (bvsalud.org)
Foxp3 inhibition with P60 enhanced chemosensitivity and reduced cell survival and migration in human and murine breast tumor cells. (bvsalud.org)
High-throughput profiling of soluble factors in plasma identifies fractalkine (FKN), a chemokine involved in immune chemotaxis and adhesion, as a biomarker of response to immunotherapy that also correlates with myeloid cell diversity in human patients and murine models. (bvsalud.org)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
In 2010, renal organoids were produced from murine fetus-derived kidney stem cells [15] . (danabiosci.com)

Notch signaling has been implicated in many microenvironmental processes associated with cancer progression such as vascular remodeling, immunosuppression, epithelial to mesenchymal transition (EMT), as well as maintenance of the CSC pool 21 . (nature.com)

A wide variety of hematopoietic cells exist, with the major protective lineages being lymphoid and myeloid. (justia.com)
In other cases, where certain cells are lacking, such as islets of Langerhans in the case of diabetes, or cells which secrete dopamine in the case of Parkinson's disease, or bone marrow cells in various hematopoietic diseases, or muscle cells in muscle wasting disease, or retinal epithelial cells in visual disorders, it would be desirable to be able to provide cells which could fulfill the desired function. (justia.com)

We compared microRNA expression patterns in three cisplatin resistant sublines derived from paternal cisplatin sensitive germ cell tumor cell lines in order to improve our understanding of the mechanisms of cisplatin resistance. (biomedcentral.com)
We created an in vitro model of acquired cisplatin resistance by long term exposure of three well established germ cell tumor cell lines to cisplatin, resulting in sublines with significantly increased resistance to cisplatin. (biomedcentral.com)
PHD2 is certainly the major prolyl hydroxylase that adjusts HIF-1 proteins balance in nonhypoxic cells, whereas knockdown of PHD1 or PHD3 will not really affect HIF-1 proteins amounts in many tumor cell lines (Berra range 350C1700 at a quality of 30,000. (forgetmenotinitiative.org)
The identification of CSCs was achieved by isolating highly tumorigenic tumor cells from primary tumors and tumor cell lines. (oncotarget.com)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

We found that pharmacological inhibition of the acid loader chloride/bicarbonate anion exchanger 2 (Ae2), with 4,4'-diisothiocyanatostilbene-2,2'-disulfonicacid (DIDS) enhancedCD4(+) andCD8(+) T cell function upon TCR activation in vitro, especially under low pH conditions. (unav.edu)
Notably, genetic Ae2 silencing in OVA-specificT cells improvedCD4(+)/CD8(+) T cell function in vitro as well as their antitumor activity in vivo. (unav.edu)
Similarly, genetic modification of OVA-specificT cells to overexpress Hvcn1, a selectiveH(+) outward current mediator that prevents cell acidification, significantly improved T cell function in vitro, even at low pH conditions. (unav.edu)
Here we show that suboptimal T cell receptor stimulation of CD8+ T cells upregulates FOXP3 in vitro. (unav.edu)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Inhibition of endogenous Cripto-1 by lentivirus-mediated RNAi silencing technique suppressed NPC cell growth and invasion in vitro . (biomedcentral.com)
Scientifically speaking, organoids are in vitro generated three-dimensional (3D), mini-cluster of cells that highly simulate the structure and function of the corresponding organ in vivo [1] . (danabiosci.com)
We have now pre viously shown that FE65 improved cell surface ranges of ApoER2 in vitro. (srcpathway.com)
On this review, we examined the results of FE65 on VLDLR trafficking and processing and found that FE65 increases VLDLR within the cell surface in vitro, much like the result of FE65 on ApoER2 trafficking. (srcpathway.com)

P19 is a type of pluripotent embryonal carcinoma cells originally obtained from teratocarcinoma developed in a C3H/He mouse. (cytion.com)
These mouse carcinoma cells possess an epithelial-like morphology. (cytion.com)
P19 embryonic carcinoma cell line is adherent. (cytion.com)
A humidified incubator set at 37°C with a 5% CO2 supply is essential to growing and culturing the P19 embryonic carcinoma cell line. (cytion.com)
Pluripotent embryonic carcinoma cell line P19 is a useful model for studying early embryonic development. (cytion.com)
BACKGROUND & AIMS: Vaccination with tumor-associated antigen-pulsed dendritic cells leads to specific T-cell response against hepatocellular carcinoma. (bvsalud.org)
High regulatory T-cell count is associated with poor prognosis and seems to mediate immune tolerance in hepatocellular carcinoma. (bvsalud.org)
However, P60 enhanced the antitumoral effect of a vaccination with alpha-fetoprotein-expressing dendritic cells in established subcutaneous and orthotopic hepatocellular carcinoma characterized by high Treg levels (p = .011). (bvsalud.org)
This emphasizes the importance of regulatory T-cells inhibition for obtaining an effective antitumoral immune response in hepatocellular carcinoma. (bvsalud.org)
Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells. (cusabio.com)
Results identified elevated CR-1 expression in esophageal squamous cell carcinoma (ESCC) specimens which correlated to poor prognosis of the patients. (cusabio.com)

A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. (mdpi.com)
Moreover, in vivo , the proliferation of the tumor cells was evaluated in xenotransplant nude mice model with whole-body visualizing instrument. (biomedcentral.com)
Sphere formation, cell proliferation, colony formation in soft agar, and xenograft tumor formation were analyzed. (oncotarget.com)

Abnormal cell division is a hallmark of tumor cells. (the-scientist.com)

We conducted a systematic review of the literature on the effects of vitamin D on ovarian cancer cell. (mdpi.com)
The two investigated ovarian cancer cell lines showed a distinctive difference in the number of expressed MMPs (2 vs. 10). (biomedcentral.com)

It was not until 1998 that American biologist James Thomson isolated and cultured human embryonic stem cells from human blastocysts for the first time [11] . (danabiosci.com)

The high metabolic activity and insufficient perfusion of tumors leads to the acidification of the tumor microenvironment (TME) that may inhibit the antitumor T cell activity. (unav.edu)
Our results suggest that Foxp3 exerts protumoral intrinsic effects in breast cancer cells and that gene-therapy-mediated blockade of Foxp3 could constitute a therapeutic strategy to improve the response of these tumors to standard treatment. (bvsalud.org)
According to the hypothesis, only a small group of stem cell-like tumor cells can form new tumors. (biomedcentral.com)
But nonviral tumors, which are composed of aberrant host cells, do not provide such targets, and the immune response is suppressed by defenses against autoimmune reactions. (the-scientist.com)
As a result, these liver organ immune system cells, including B cells and their cytokines, mainly become innate immune effectors against tumors and infections simply by their T helper-1 immune response in the liver organ. (enmd-2076.com)
Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
Our approach was designed to characterize the role of micro-RNAs on the presumably multifactorial phenomenon of acquired cisplatin resistance in germ cell tumors. (biomedcentral.com)

This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. (bdbiosciences.com)
So as a new therapeutic approach, stem cells are under research with a focus on reducing the burden of several kidney diseases. (biomedcentral.com)

When the host lacks the ability to raise a defense against a particular disease, there may also be an interest in administering specific T-cells or B-cells or precursors thereof which may supplement the host's compromised immune system. (justia.com)

revealed that lung organoids can be produced from the 3D coculturing of endothelial cells and adult bronchioalveolar stem cells [17] . (danabiosci.com)

The cells at the exterior then deposit extracellular matrix (ECM), containing collagen IV and laminin, similar to the composition and structure of basement membrane. (wikipedia.org)
Tumor cells are surrounded by the extracellular matrix (ECM) comprising of proteoglycanes and non-proteoglycanic matrix components (collagen, laminin, fibronectin and elastin). (biomedcentral.com)

The pluripotent cell types that comprise embryoid bodies include embryonic stem cells (ESCs) derived from the blastocyst stage of embryos from mouse (mESC), primate, and human (hESC) sources. (wikipedia.org)
CFC1, which functions in the development of embryos and decides the left-right axis, was strongly expressed in sphere-forming cells only and was related to the unfavorable prognosis of NB patients. (oncotarget.com)

The immune system which results from cells of the lymphoid and myeloid lineages is developed in vivo, so as to recognize self from non-self. (justia.com)

In contrast to monolayer cultures, however, the spheroid structures that are formed when ESCs aggregate enables the non-adherent culture of EBs in suspension, making EB cultures inherently scalable, which is useful for bioprocessing approaches, whereby large yields of cells can be produced for potential clinical applications. (wikipedia.org)
Additionally, although EBs largely exhibit heterogeneous patterns of differentiated cell types, ESCs are capable of responding to similar cues that direct embryonic development. (wikipedia.org)
In addition, technologies have also been developed to physically separate cells by forced aggregation of ESCs within individual wells or confined on adhesive substrates, which enables increased throughput, controlled formation of EBs. (wikipedia.org)

Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The importance of apoptosis is exemplified by the vertebrate adaptive immune system, in which B lymphocytes, as they differentiate from their precursor cells, rearrange the DNA of immunoglobulin genes to generate a vast number of potential antibodies. (the-scientist.com)
It is therefore of interest to find effective ways to produce cells which may function, proliferate, and differentiate as appropriate, while being safe from attack by a recipient's immune system. (justia.com)
In 2009, Hans Clevers and colleagues first demonstrated that single Lgr5 + -expressing intestinal adult stem cells (ASCs) could self-organize and differentiate to form intestinal crypt-villus structures that contained all intestinal cell types [14] . (danabiosci.com)

Role of Otx2 in Epiblast Stem Cells (EpiSCs): Otx2 stabilizes the primed EpiSC state by suppressing mesendoderm into neural fate switch in cooperation with BMP4 and Fgf2. (cnr.it)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

The aim of this study was to analyze the roles of Cripto-1 in the progression and clinical characteristics in NPC clinical samples and cell lines. (biomedcentral.com)
Because in order to avoid renal injury, the recovery of affected cells is as significant as preventing its progression towards end stage renal disease [ 1 ]. (biomedcentral.com)

4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
She is the George Barth Geller Professor and Chair of the Department of Cell Biology at Duke University Medical Center. (biologists.com)

Human Cripto-1, also known as teratocarcinoma-derived growth factor-1 (TDGF-1), is a member of the Epidermal growth factor-cripto FRL1 cryptic (EGF-CFC) family (Cripto in humans, FRL1 in Xenopus, and Cryptic in mice), which is indispensable for early embryonic development[ 4 ]. (biomedcentral.com)

In that same study, we observed that FE65 increased sApoER2 and ApoER2 CTF in COS7 cells, whilst knockdown of FE65 brought on decreased ApoER2 CTF in vivo. (srcpathway.com)
The knockdown and overexpression of CFC1 were performed using a lentiviral system in NB cell lines. (oncotarget.com)

The field of the subject invention is the use of major histocompatibility complex antigen lacking cells and organs which may serve as universal donors in cellular and organ therapies including transplantation and to produce chimeric non-human mammals. (justia.com)
Expression analysis of the human Hox Complex in teratocarcinoma cells (N-tera2/D1) upon treatment with retinoic acid and the definition of temporal colinearity concept. (cnr.it)
Molecular mechanism controlling the expression of the HOX Complex gene family in human teratocarcinoma cells (N-tera2/D1). (cnr.it)

CR-1 high cells isolated from ESCC cells possess cancer stem-like cells (ECSLC) properties. (cusabio.com)

Researchers use P19 cells to elucidate cell signaling pathways and cellular and molecular mechanisms involved in these processes. (cytion.com)
These Wnts were chosen since previous studies suggest that C57mg cells respond differently to these Wnts, and since these Wnts can activate different signaling pathways in other systems. (biomedcentral.com)

fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

These originate in the plasma cells of bone marrow. (solutionpharmacy.in)
These cancers affect the bone marrow which is the site for blood cell production. (solutionpharmacy.in)
When cancerous, the bone marrow begins to produce excessive immature white blood cells that fail to perform their usual actions and the patient is often prone to infection. (solutionpharmacy.in)

RESULTS: In a preventive setting prior to tumor engraftment, vaccination with alpha-fetoprotein-expressing dendritic cells significantly decreased tumor growth in a subcutaneous model (p = .0256), but no further effects were achieved by addition of P60. (bvsalud.org)

In this study we investigated the expression pattern of the 23 MMPs, currently known in humans, in different gynecological cancer cell lines. (biomedcentral.com)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
Cancer stem cells (CSCs) are considered to be critically involved in the recurrence and metastasis of NB and are isolated as NB spheres. (oncotarget.com)
Therefore, CSCs have become promising target cancer cells for preventing cancer relapse and improving the prognosis of tumor patients [ 3 ]. (oncotarget.com)

Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)

The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In order to find more promising treatments to stimulate renal repair, stem cell-based technology has been proposed as a potentially therapeutic option. (biomedcentral.com)

This Special Issue contains separate papers discussing undervalued ubiquitous proteins, chronic inflammation, eicosanoids, microbiome and morbid obesity, PCN, cell transition, followed by altered signaling induced by Metformin, NF-κB signaling and crosstalk during carcinogenesis, and a brief synopsis. (4open-sciences.org)

In vivo, DIDS administration delayed B16OVA tumor growth in immunocompetent mice as monotherapy or when combined with adoptive T cell transfer of OVA-specificT cells. (unav.edu)
The cell pallet is again resuspended, and cells are poured into the new flask for growth. (cytion.com)
Secreted FKN inhibits lung adenocarcinoma growth in vivo through a prominent contribution of systemic effector NK cells and increased tumor immune infiltration. (bvsalud.org)
After the inhibition of the endogenous Cripto-1, the growth, cell cycle and invasion of cells were detected by MTT, FACS and Boyden chamber assay respectively. (biomedcentral.com)
Results: The overexpression of CFC1 increased sphere formation, cell growth, and colony formation. (oncotarget.com)

Alternatively, to avoid dissociation into single cells, EBs can be formed from hESCs by manual separation of adherent colonies (or regions of colonies) and subsequently cultured in suspension. (wikipedia.org)
Is P19 an adherent cell line? (cytion.com)
Adherent cells are washed with 1 X phosphate buffer saline and incubated with Accutase until the cells dissociate. (cytion.com)
Adherent and semi-confluent layer of P19 cells at 10× and 20× magnification. (cytion.com)
Methods: The gene expression profiling of adherent (control) and sphere-forming primary NB cells was conducted using a gene expression microarray. (oncotarget.com)

This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
In mammals, the immune systems serves as the primary defense with many different types of cells and mechanisms to protect the host. (justia.com)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
It includes the possible stem cell therapy mechanisms involved and outcomes recorded so far, the limitations of using these regenerative medicines, and the progressive improvement in stem cell therapy by adopting approaches like PiggyBac, Sleeping Beauty, and the Sendai virus. (biomedcentral.com)

Knowing about a cell line's general characteristics and origin is essential before you start working with it. (cytion.com)
The P19 cell line is widely cultured in research laboratories due to its unique characteristics. (cytion.com)
The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. (bdbiosciences.com)

The improvement of cell culture conditions through mimicking the in vivo microenvironment was made in 1987. (danabiosci.com)

In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs. (justia.com)
This type of cancer originates from the epithelial layer of cells that form the lining of external parts of the body or the internal linings of organs within the body. (solutionpharmacy.in)
90% of cancer-related deaths, is a multi-step process involving cancer cell intravasation into the bloodstream, dissemination to distant sites, and seeding in new organs 1 . (nature.com)
Stem cells are most frequently cultured in Matrigel and in the presence of suitable exogenous factors including chemical small molecule inhibitors/activators, cytokines, and medium additives, and can be coaxed into forming organoids of corresponding organs. (danabiosci.com)

Normal function is to regulate cell division. (the-scientist.com)
Beta-catenin then accumulates in the nucleus where it interacts with its transcriptional co-activators, members of the Lymphoid Enhancer Factor/T Cell Factor (LEF/TCF) family of high mobility group DNA binding proteins to regulate gene expression [ 1 ]. (biomedcentral.com)
Actin filaments play an necessary function in cell motion, and many posttranslational adjustments regulate actin filament set up. (forgetmenotinitiative.org)

Different Wnts have distinct effects on gene expression in a single cell line. (biomedcentral.com)

Similarly, it opens a new path for the treatment and repair of damaged renal cells. (biomedcentral.com)

As hESC undergo apoptosis when cultured as single cells, EB formation often necessitates the use of inhibitors of the rho associated kinase (ROCK) pathway, including the small molecules Y-27632 and 2,4 disubstituted thiazole (Thiazovivin/Tzv). (wikipedia.org)
In response to the ECM deposition, EBs often form a cystic cavity, whereby the cells in contact with the basement membrane remain viable and those at the interior undergo apoptosis, resulting in a fluid-filled cavity surrounded by cells. (wikipedia.org)
Yet another group of genes associated with cancer formation regulates programmed cell death, or apoptosis. (the-scientist.com)

Besides, this improvement was also observed by the depletion or inhibition of T regulatory cells. (unav.edu)
CONCLUSION: In this study, we showed that vaccination with alpha-fetoprotein-expressing dendritic cells in combination with a specific inhibition of regulatory T-cells by using P60 leads to synergistic tumor inhibition and prolonged survival. (bvsalud.org)

mouse and cell depletion tests demonstrated that NKT cells and NK cells weren't mixed up in hepatic damage. (enmd-2076.com)

Frozen P19 cell vials should be stored at below -150°C temperature in a freezer or the vapour phase of liquid nitrogen to maintain the viability of cells for the longer term. (cytion.com)
CM-1 or CM-ACF media can be used to freeze P19 cells using a slow freezing method that protects cells from any shock and preserve their viability. (cytion.com)
We also developed an adenoviral vector encoding P60 (Ad.P60) that efficiently transduced breast tumor cells, reduced cell viability and migration, and improved the cytotoxic response to cisplatin. (bvsalud.org)

Cisplatin upregulated Foxp3 expression in HER2+ and triple-negative breast cancer (TNBC) cells. (bvsalud.org)
High-dimensional systemic immune profiling is performed in a cohort of non-small-cell lung cancer patients undergoing PD-L1/PD-1 blockade immunotherapy. (bvsalud.org)
Therefore, investigations on testing and maintaining the integrity of genomic DNA within precancerous stem cells (pCSCs), which are within the framework of the cancer stem cell (CSC) hypothesis, are necessary. (biomedcentral.com)
Their normal function is not to cause cancer but to participate in the regulation of normal cell division. (the-scientist.com)
While the three cervical cancer cell lines expressed 10-14 different MMPs, the median expression in endometrial and choriocarcinoma cells was 7 different enzymes. (biomedcentral.com)
All types of cancers are common, in that, the cancer cells are abnormal and multiply out of control. (solutionpharmacy.in)
Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
Only the has-miR-10b involved in breast cancer invasion and metastasis and has-miR-512-3p appeared to be up-regulated (2-3-fold) in all three cell lines. (biomedcentral.com)

Can precancerous stem cells be risk markers for malignant transformation in the oral mucosa? (biomedcentral.com)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

The adoptive transfer and cytotoxic assay against principal cultured hepatocytes verified the function of Compact disc11b+ Kupffer cells in CCl4-induced hepatitis. (enmd-2076.com)
Co-IP assays Cells had been lysed in altered radioimmunoprecipitation assay stream, and WCLs had been incubated over night with anti-FLAG (Sigma-Aldrich, St. Louis, MO), anti-PHD2 (Novus Biologicals, Littleton, Company), anti-PHD3 (Novus Biologicals), or anti-hydroxyproline (Abcam) antibody in the existence of proteins ACagarose beans (Novus Biologicals). (forgetmenotinitiative.org)

Stem cell research began to flourish. (danabiosci.com)

In this article, we will discuss the fundamentals of mouse-derived P19 cells. (cytion.com)
No. 554680, dashed line histogram) or PE Mouse Anti-Human Podoplanin (Cat. (bdbiosciences.com)
Cell culture and drug treatment method conditions Human neuron committed teratocarcinoma, mouse motor neuron derived cells and SMA type I pa tient fibroblasts were maintained in regular problems in Dulbeccos modified Eagle medium supplemented with 10% fetal calf serum, 1% antibiotics and two mM glutamate. (srcpathway.com)
Rabbit polyclonal antibodies (pAb) were used for preparing a gold-pAb conjugate, and the rGroEL-specific mouse monoclonal antibody was used as the antigen detection reagent at the ICT test line. (jsce-ip.com)

The immunohistochemical evaluation from the liver organ and the stream cytometry from the liver organ mononuclear cells demonstrated that clodronate liposome (c-lipo) treatment significantly reduced the spindle-shaped F4/80+ or Compact disc68+ cells, as the oval-shaped F4/80+ Compact disc11b+ cells elevated. (enmd-2076.com)
Furthermore, anti-TNF Ab pretreatment (which reduced the FasL appearance of Compact disc11b+ Kupffer cells), anti-FasL Ab mice or pretreatment attenuated the liver organ injury induced by CCl4. (enmd-2076.com)
It really is popular that liver organ HGFB NK cells and NKT cells potently generate IFN- in response to IL-12 and/or LPS [11]C[13]. (enmd-2076.com)
Oddly enough, liver organ B cells (mainly B-2 cells) generate IL-12 and IFN- however, not IgM, in response to LPS (vice versa for spleen B cells) [14]. (enmd-2076.com)
Furthermore, these IL-12-making liver organ B cells, as opposed to spleen B cells, phagocytose bacterias and eliminate them [15], [16]. (enmd-2076.com)

Thereafter, organoids were constructed and employed as a novel research model that could be used instead of traditional cell lines and heterogeneous animal models. (danabiosci.com)

We studied the role of the expression of the transcription factor FOXP3 on CD8+ T cell function and anti-tumor immunity. (unav.edu)
Flow cytometric analysis of human podoplanin expression on human teratocarcinoma cell line. (bdbiosciences.com)
Lentiviral vectors were constructed to get an efficient expression of anti-Cripto-1 siRNA in CNE-2 and C666-1 cells, with invalid RNAi sequence as control. (biomedcentral.com)
The results of real-time RT-PCR and western blot showed that the expression level of Cripto-1 was markedly higher in NPC cell lines than that in the immortalized nasopharyngeal epithelial cell at both mRNA and protein levels. (biomedcentral.com)

Anatomy51

Organisms14

Diseases16

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes57

Disciplines and Occupations1

Information Science3

Differentiation29

Carcinomas4

NCCIT3

Mammary4

Tissue7

Pluripotent stem6

Tissues3

Murine7

Mesenchymal1

Hematopoietic2

Tumor cell lines4

Progenitor cells1

Vitro10

Carcinoma11

Proliferation3

Hallmark of tumor cells1

Ovarian cancer cell2

Human embryonic stem1

Tumors7

Kidney2

Precursors1

Endothelial cells1

Extracellular2

Embryos2

Lineages1

ESCs3

Differentiate5

Neural2

Progression2

Biology3

Embryonic development1

Knockdown2

Complex3

Possess1

Pathways2

Isolation1

Bone marrow3

Subcutaneous1

Humans2

CSCs5

Phenotype1

Therapeutic2

Transition1

Growth5

Adherent5

Mechanisms4

Characteristics3

Microenvironment1

Organs4

Regulate3

Gene expression1

Renal1

Apoptosis3

Inhibition2

Depletion1

Viability3

Cancer9

Markers2

Assay2

Stem Cell Res1

Mouse4

Organ5

Heterogeneous1

Expression4