• The main outcome measure was the mean number of lens epithelial cells attached to each lens type after 24 hours of incubation. (arvojournals.org)
  • Coating the lenses with fibronectin caused a significant increase in attachment of lens epithelial cells for all three lens types. (arvojournals.org)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • While Akt Ser 473 phosphorylation was readily detectable in AND-34 +/+ lens epithelial cells, it was markedly reduced in the AND-34 −/− lens epithelium. (molvis.org)
  • These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
  • Fibronectin also binds to other extracellular matrix proteins such as collagen, fibrin, and heparan sulfate proteoglycans (e.g. syndecans). (wikipedia.org)
  • Fibronectin also contains domains for fibrin-binding (I1-5, I10-12), collagen-binding (I6-9), fibulin-1-binding (III13-14), heparin-binding and syndecan-binding (III12-14). (wikipedia.org)
  • Deposition of collagen and proliferation of smooth muscle cells are the response to chronic inflammation and injury of the submucosa leading to fibrosis and thickening of the submucosa and the smooth muscle layers of the bowel wall. (bmj.com)
  • Immunohistochemical staining was performed using antibodies against the major components of basement membranes such as laminin, heparan sulfate proteoglycan (HSPG), Collagen IV and fibronectin. (thieme-connect.com)
  • Up-regulated genes consist of those encoding protein advertising cell migration and invasion like the mesenchymal cell-cell adhesion proteins N-cadherin the intermediate filament proteins vimentin as well as the extracellular matrix protein fibronectin Gimeracil and collagen. (bioskinrevive.com)
  • Primary cultures of human corneal epithelial cells cultivated on BSA (number of replicates: 2), Collagen type I (number of replicates: 2) and Collagen type IV (number of replicates: 2) matrix. (nih.gov)
  • Adherence was altered in one of the three cancer cell lines to the extracellular matrix proteins collagen type IV and fibronectin. (dcu.ie)
  • Furthermore, the knockdown of Sp1 inhibited the expression of collagen type I (CoL-I) and fibronectin (Fn). (ijbs.com)
  • This study was undertaken to localize fibronectin and type IV collagen in the cultured retinal pigment epithelial cell by means of immunofluorescent staining and immunocytochemical methods. (ekjo.org)
  • Immunofluorescent staining and immunocytochemical methods revealed fibronectin and type IV collagen localized on the extracellular membrane of the cultured retinal pigment epithelial cell. (ekjo.org)
  • This study demonstrated that fibronectin and type IV collagen are an integral component of the extracellular matrix of the human retinal pigment epithelial cell in vitro. (ekjo.org)
  • Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)
  • Immunization of type VII collagen in athymic nude SJL mice did not induce an autoimmune response, whereas the repletion of T cells from type VII collagen-immunized wild-type mice to the thymic mice showed autoantibody production and resulted in a blistering disease phenotype, supporting the role of T cells in the induction of epidermolysis bullosa acquisita. (medscape.com)
  • Epidermolysis bullosa acquisita (EBA) is an autoimmune disease, manifested by autoantibodies that target type VII collagen, the major protein of anchoring fibrils and the one that connects the epithelial basement membrane to the dermis. (medscape.com)
  • Current interest in this process, which is described as an epithelial-mesenchymal transition (EMT), stems from its developmental importance and its involvement in several adult pathologies. (rupress.org)
  • The EMT International Association was formed in 2002 to provide an international body for those interested in EMT and the reverse process, mesenchymal-epithelial transition, and, most importantly, to bring together those working on EMT in development, cancer, fibrosis, and pathology. (rupress.org)
  • The purpose of this study was to identify potential therapeutic strategies to slow down or prevent the expression of early-onset epithelial to mesenchymal transition (EMT) marker proteins (fibronectin and alpha smooth muscle actin, α-SMA) without sacrificing the synthesis and accumulation of the prosurvival protein vascular endothelial growth factor (VEGF) in cultured virally transformed human lens epithelial (HLE) cells. (molvis.org)
  • Lung wound-healing responses such as the epithelial-to-mesenchymal transition (EMT) can be reparative or pathologic processes. (cdc.gov)
  • metastatic potential of HCC cells by selectively regulating epithelial-mesenchymal transition (EMT) regulatory proteins such as N-cadherin, E-cadherin, vimentin and fibronectin. (nos-nop.org)
  • One important insight came from the discovery that the increased motility and invasiveness of cancer cells is reminiscent of the epithelial-mesenchymal transition (EMT) that occurs during embryonic development ( 2 ). (spandidos-publications.com)
  • One of the formation mechanisms is the epithelial-mesenchymal transition, in which epithelial cells acquire the phenotypic properties of mesenchymal cells. (medprep.info)
  • INTRODUCTION Epithelial-mesenchymal transition (EMT) is a transcriptional and morphological program that occurs during normal development and tissue remodeling and in the progression of diseases such as fibrosis and metastatic cancers. (bioskinrevive.com)
  • Fibronectin is a high-molecular weight (~500-~600 kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. (wikipedia.org)
  • HCC tissues derived from chemical-induced rat liver cancer models validated that miR-31 manifestation is significantly down-regulated, and that those cell cycle- and EMT-regulatory proteins are deregulated in rat liver cancer. (nos-nop.org)
  • Overall, we suggest that miR-31 functions like a tumor suppressor by selectively regulating cell cycle and EMT regulatory proteins in human being hepatocarcinogenesis providing a novel target FANCG for the molecular treatment of liver malignancies. (nos-nop.org)
  • Fibrosis is characterised by an excessive production of extracellular matrix components, and cells in the tissue are constantly interacting with these extracellular matrix proteins. (bmj.com)
  • Its major function in vivo is to activate the major in-gel proteins, seminogelin I, II, and fibronectin, in freshly ejaculated semen, which then liquefies semen through proteolytic fragmentation and releases progressively motile sperm. (mlo-online.com)
  • These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
  • The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. (justia.com)
  • In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. (justia.com)
  • The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. (microbiologyresearch.org)
  • Down-regulated genes consist of those encoding protein keeping epithelial cell-cell adhesions like the adherens junction proteins E-cadherin as well as the limited junctions protein claudins and occludin. (bioskinrevive.com)
  • Proteins were identified in the total cell lysates that suggest that DLKP-M is mesenchymal-like in nature as originally described and that the interconversion process observed between the clones is regulated at least by key proteins involved in protein metabolism. (dcu.ie)
  • To demonstrate differences in human lens epithelial cell adhesion to different intraocular lens biomaterials in vitro and to determine whether these differences can be influenced by coating the intraocular lens surface with commercially available fibronectin. (arvojournals.org)
  • Some reports suggest that the design of the IOL is important, 10 12 13 others state that the adhesive properties of the lens material may be relevant, 6 7 10 as soft acrylic IOLs show greater lens epithelial cell (LEC) adhesion than do PMMA and silicone lenses. (arvojournals.org)
  • He suggests that further ingrowth of epithelial cells is prevented by the adhesion of the IOL to the LEC and this then to the capsule. (arvojournals.org)
  • Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. (wikipedia.org)
  • Dr. Yamada's group explores how coordination between the three-dimensional (3D) extracellular matrix of connective tissue or basement membranes, integrins, signal transduction, and cytoskeleton mediates or regulates key biological processes - cell adhesion, migration, matrix remodeling, tissue morphogenesis, and cancer cell invasion. (nih.gov)
  • To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
  • Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
  • Binding of IgA prevented fungal epithelial adhesion and invasion resulting in a dampened proinflammatory epithelial response. (frontiersin.org)
  • These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)
  • Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3. (bdbiosciences.com)
  • Adhesion molecules in hematopoietic cells. (bdbiosciences.com)
  • RhoA has been implicated in the formation of stress fibers and cell adhesion in fibroblasts. (spandidos-publications.com)
  • These cells also had less α-smooth muscle actin and phosphorylated myosin light chain in cortical patches decreased abundance of the adhesion receptor CD44 at membrane protrusions and attenuated autophosphorylation of focal adhesion kinase. (bioskinrevive.com)
  • Collagens considerably suppressed expression of the α5 gene in CECs at confluence suggesting that the sustained staining for CI and CIV after corneal injury may play a role in controlling adhesion to the temporary FN matrix and further differentiation of CECs into suprabasal epithelial cells through a mechanism involving the α5β1 integrin. (nih.gov)
  • When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs adhesion. (jove.com)
  • When co-cultured with human mesenchymal stem cells, the nano-matrix exhibited excellent bioactivity in encouraging cell adhesion. (jove.com)
  • Therefore, it has the potential to serve as an injectable scaffold to repair bone fractures by promoting cell adhesion and function at the target location. (jove.com)
  • Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)
  • There are four fibronectin-binding domains, allowing fibronectin to associate with other fibronectin molecules. (wikipedia.org)
  • We demonstrate that down-modulation of Δ133p53 isoforms leads to changes in gene expression of the extracellular matrix molecules fibronectin (FN), EDA+-FN, laminin α5 and laminin α3 in human breast epithelial cells. (dundee.ac.uk)
  • As well as, because the obvious molecular weights of VWF and fibronectin are virtually similar, these molecules have been tough to tell apart by SDS-polyacrylamide gel electrophoresis adopted by immunoblotting. (holliseden.com)
  • Next, they evaluated the potential for these cells to release oxygen free radicals (reactive molecules that can damage lung cells), their capacity to break down fibronectin (a lung structural protein), and their ability to damage epithelial cells in tissue culture. (healtheffects.org)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • The RGD sequence (Arg-Gly-Asp) is located in III10 and is the site of cell attachment via α5β1 and αVβ3 integrins on the cell surface. (wikipedia.org)
  • Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. (bdbiosciences.com)
  • We beforehand demonstrated that Arg-Gly-Asp (RGD)-containing ligand-mimetic inhibitors of integrins are unable to dissociate pre-formed integrin-fibronectin complexes (IFCs). (holliseden.com)
  • MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
  • IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). (rndsystems.com)
  • An analysis of the basement membrane, which can modulate epithelial proliferation, reveals a dysfunction of this layer between cholesteatoma and stroma. (thieme-connect.com)
  • It is secreted by various cells, primarily fibroblasts, as a soluble protein dimer and is then assembled into an insoluble matrix in a complex cell-mediated process. (wikipedia.org)
  • Fibroblasts secrete proteases, including matrix metalloproteinases, that digest the plasma fibronectin, and then the fibroblasts secrete cellular fibronectin and assemble it into an insoluble matrix. (wikipedia.org)
  • The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. (bdbiosciences.com)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • The effect of Mast cell Tryptase to the Expression of Eotaxin-1/CCL11, Eotaxin-2/CCL24 and Eotaxin-3/CCL26 by Conjunctival Fibroblasts. (nii.ac.jp)
  • It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. (nature.com)
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
  • Corneal dystrophies commonly associated with RCES include epithelial basement membrane (Cogan's microcystic or map dot fingerprint dystrophy), Reis-Buckler's, lattice, and granular dystrophies. (bmj.com)
  • OVDs can create and preserve surgical space, pressurize the anterior chamber in the presence of an open wound, partition spaces, protect the corneal endothelial cells from fluid turbulence, and facilitate surgery. (lww.com)
  • In this study, we examined whether expression of the gene encoding the α5 subunit from the FN binding integrin α5β1 changes as corneal epithelial cells (CECs) are cultured in the presence of collagens. (nih.gov)
  • All mast cells in the muscularis propria were colocalised with patches of laminin. (bmj.com)
  • In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
  • The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (bmj.com)
  • When primary isolated human bronchial epithelial cells were treated with 1-100 nM MaR1, transforming growth factor (TGF)-B1-induced EMT processes were altered. (cdc.gov)
  • Furthermore, when conditioned medium from DHA-treated MSCs was used to culture bronchial epithelial cells, the epithelial cells exhibited modulation of protease activation. (cdc.gov)
  • Our results identify DHA and MaR1 as regulators of reparative processes after lung inflammation and wounding, including effects on two pivotal cellular players-bronchial epithelial cells and MSCs. (cdc.gov)
  • These cell lines include a lung adenocarcinoma (A549), a non small cell lung carcinoma cell line (DLKP), normal bronchial epithelial cell line (NHBE), a breast adenocarcinoma (MCF-7) and human mammary epithelial cells (HMEC). (dcu.ie)
  • Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. (microbiologyresearch.org)
  • While platelets play a crucial role in clot formation during hemostasis, inflammatory cells débride injured tissue during the inflammatory phase. (medscape.com)
  • Chemokines released by platelet activation attract inflammatory cells to the area, leading to the next phase in the healing process. (medscape.com)
  • In addition to activation of fibrin, thrombin activated by the coagulation cascades facilitates migration of inflammatory cells to the site of injury by increasing vascular permeability. (medscape.com)
  • Vasodilation is an important means by which the wound can be exposed to increased blood flow, accompanied by the necessary inflammatory cells and factors that fight infection and débride the wound of devitalized tissue. (medscape.com)
  • Renal interstitial fibrosis (RIF) is a common pathological feature of progressive injury in CKD and predominantly includes renal tubular epithelial cell atrophy, inflammatory cell infiltration, and the excessive deposition of extracellular matrix (ECM) [ 2 , 3 ]. (ijbs.com)
  • Inflammatory macrophage-enriched populations from quartz-exposed lung had the ability to cause fibronectin degradation but could not cause detachment injury to epithelial cells over and above that caused by control alveolar macrophages. (hw.ac.uk)
  • Basement membrane regulates fibronectin organization using sliding focal adhesions driven by a contractile winch. (nih.gov)
  • Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions. (nih.gov)
  • Btbd7 regulates epithelial cell dynamics and branching morphogenesis. (nih.gov)
  • Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
  • Recently, we reported that nuclear β-catenin, but not HIF-2α, regulates the expression of fibronectin and α-SMA in atmospheric oxygen. (molvis.org)
  • The fact that and are up-regulated in HCC led us to hypothesize that normal and expressions are balanced by endogenous miR-31, which selectively regulates and mRNA translation in normal hepatic liver cells. (nos-nop.org)
  • The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. (cancerindex.org)
  • In contrast, fibronectin demonstrated an intensive staining especially in the stroma adjacent to the basement membrane. (thieme-connect.com)
  • This staining pattern resembles the initial rear-rangement of the epithelial basement membrane in human skin wounds and provides evidence for a lost contact inhibition on the provisional fibronectin-layer as a reason for the hyperproliferative nature of cholesteatoma lesions. (thieme-connect.com)
  • It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. (bvsalud.org)
  • Next, to verify that miR-31 particularly binds to 3UTRs of also to interfere translation of these transcripts, mutant vectors harboring random mutation sequences of miR-31 biding sites from the 3UTR of and genes had been generated, and each vector was co-transfected with miR-31 into SNU-449 and SKHep-1 cells. (nos-nop.org)
  • This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. (cancerindex.org)
  • Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
  • The two events, while occurring simultaneously but independently, likely provide a disadvantageous situation in which the newly emerging mesenchymal cell population is more likely to be resistant to apoptosis than the epithelial cell population from which the mesenchymal cell population stemmed. (molvis.org)
  • Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. (bdbiosciences.com)
  • Each fibronectin subunit has a molecular weight of ~230-~275 kDa and contains three types of modules: type I, II, and III. (wikipedia.org)
  • The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. (bmj.com)
  • Ultrastructural immunocytochemical technique also revealed fibronectin associated with extracellular tissue. (ekjo.org)
  • Along with fibrin, plasma fibronectin is deposited at the site of injury, forming a blood clot that stops bleeding and protects the underlying tissue. (wikipedia.org)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • Notably, the F8(scFv)-DAAO-Q144R conjugate generates a stronger cytotoxicity to tumor cells than the native enzyme, particularly when an inhibitor of HO-1 is used, making it a promising candidate for a selective antitumor oxidative remedy managed by the substrate addition, within the so known as "exercise on demand", thus sparing regular tissue from injury. (holliseden.com)
  • Drs. Kenneth Donaldson and coworkers at the Institute of Occupational Medicine examined whether exposure to ozone activates white blood cells to release substances that can damage lung tissue. (healtheffects.org)
  • Fibronectin damage in vivo could be an important factor in disordering the connective tissue scaffold of the lung, thereby favoring fibrosis. (hw.ac.uk)
  • However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
  • The present data provide evidence that Snail promotes tumor cell motility and angiogenesis which is mainly mediated through the regulation of RhoA activity. (spandidos-publications.com)
  • Tumor cell motility is the hallmark of invasion and an essential step in metastasis. (spandidos-publications.com)
  • Cellular fibronectin is assembled into the extracellular matrix, an insoluble network that separates and supports the organs and tissues of an organism. (wikipedia.org)
  • Dr. Yamada's research emphasizes the characterization of the dynamic movements of cells and their extracellular matrix as tissues are remodeled in 3D in real time. (nih.gov)
  • The biological systems studied include human primary cells migrating in 3D, human tumor cells and tissues, and mouse organ development (salivary glands, lungs, and kidneys). (nih.gov)
  • The F8(scFv)-DAAO-Q144R from E. coli cells is absolutely soluble, exhibits a excessive particular exercise, is extra thermostable in blood than the native DAAO, possesses a binding affinity for EDA properly suited to environment friendly tumor accumulation, and localizes in tumor tissues. (holliseden.com)
  • Durotaxis by human cancer cells. (nih.gov)
  • Tyrosine kinase inhibitors (TKIs) against the human epidermal growth factor receptor (EGFR) are now standard treatment in the clinic for patients with advanced EGFR mutant non-small-cell lung cancer (NSCLC). (researchgate.net)
  • Human lens epithelial (HLE) cells survive under hypoxia through complex and interactive signal transduction pathways whose mechanisms of action are not well understood. (molvis.org)
  • Interestingly, MaR1 also regulated the actions of primary isolated human lung mesenchymal stem (stromal) cells (MSCs). (cdc.gov)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. (rndsystems.com)
  • Mature mouse IL-22BP consists of two tandem Fibronectin type-III domains and shares 85% and 68% amino acid sequence identity with rat IL-22BP and the short isoform of human IL-22BP, respectively (6, 14). (rndsystems.com)
  • The involvement of aberrant AID expression in human gingival epithelial cells with Streptococcus anginosus antigen on the mechanisms of oral cancer. (nii.ac.jp)
  • Extracellular matrix of the human retinal pigment epithelial cells in vitro. (ekjo.org)
  • In this study, human pancreatic islet-derived 1.1B4 cells were exposed to H2O2 and analysed using a human microarray, which revealed that heme oxygenase 1 (HMOX1), glutamate-cysteine ligase, early growth response 1, nuclear receptor subfamily 4 group A member 3 (NR4A3) and jun B proto-oncogene were upregulated, whereas superoxide dismutase 1 and catalase were not. (bvsalud.org)
  • Mesenchymal cells are able to actively secrete components of the extracellular matrix - collagens, fibronectin, which can contribute to the formation of a scar. (medprep.info)
  • On the other hand actin filaments in transdifferentiated mesenchymal cells are bundled Gimeracil into heavy contractile stress materials in the ventral cell surface area. (bioskinrevive.com)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • miR-203 drives breast cancer cell differentiation. (medscape.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
  • Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)
  • Cholesteatoma is a disease with an epithelial State of hyperproliferation based on a disturbed cell-matrix interaction. (thieme-connect.com)
  • In contrast, other evidence suggests that both receptors may contribute to modulation of transformed colon cell behavior, with a potential to promote cancer progression and/or chemoresistance. (bvsalud.org)
  • Current evidence suggests that specific ligands of both AhR and PXR can protect intestinal epithelium against inflammation and assist in the maintenance of epithelial barrier integrity. (bvsalud.org)
  • The current results indicated that B[a]P seems to induce EMT in lung cancer cells, and it also may drive disease progression in patients with lung cancer. (nih.gov)
  • Bioactive lipid mediators regulate lung epithelial and mesenchymal cell reparative processes in vitro. (cdc.gov)
  • There are two main subcategories of lung cancer: nonsmall cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (hindawi.com)
  • Here the proteomic alterations induced by IC8o 5-FU treatment of normal and cancer cells of epithelial origin of the lung and breast are investigated. (dcu.ie)
  • this process could aid leukocyte lung cell contact, allowing epithelial injury to be expressed, and could also be a factor leading to pathological change. (hw.ac.uk)
  • Breast metastatic tumors in lung can be substituted by lung-derived malignant cells transformed by alternative splicing H19 lncRNA. (medscape.com)
  • Single-cell RNA-seq identifies cell diversity in embryonic salivary glands. (nih.gov)
  • METHODS Mast cells were visualised by immunohistochemical staining of the mast cell specific proteases chymase and tryptase. (bmj.com)
  • No colocalisation of mast cells was found with fibronectin or vitronectin. (bmj.com)
  • CONCLUSIONS The large accumulation of mast cells in the muscle layer of strictured bowel suggests a functional role for these cells in the hypertrophic and fibrotic response of the smooth muscle cells. (bmj.com)
  • In fibrosing colonopathy, a complication observed in children with cystic fibrosis treated with high strength pancreatic enzyme replacement therapy, an accumulation of mast cells was observed in the intestinal strictures. (bmj.com)
  • These studies provide evidence that mast cells with their plethora of mediators may be involved in intestinal fibrosis, a notion supported by the observation of mast cell hyperplasia and activation in a variety of clinical and experimental situations in which fibrosis is prominent. (bmj.com)
  • However, liver cirrhosis and intestinal stricture formation are not common in mast cell disease, indicating that besides mast cells additional factors or specific stimulation of mast cells may be necessary to induce severe fibrosis. (bmj.com)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
  • This second class of mAbs was additionally distinguished from 13, 4B4, and AIIB2 by their potential to induce homotypic cell aggregation. (holliseden.com)
  • Phenotypes were characterised and 5-FU was found to induce and reduce invasion in various cell lines. (dcu.ie)
  • The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms. (wikipedia.org)
  • Cells that do not express detectable Δ133p53 isoforms or express low levels of these isoforms failed to form polarized structures in 3D. (dundee.ac.uk)
  • Activating the nuclear piston mechanism of 3D migration in tumor cells. (nih.gov)
  • The five yr Anisole Methoxybenzene survival rate was significantly decreased in patient with low level of miR-31 manifestation in the tumor cells (Log-rank = 0.0015*) (C) The qRT-PCR analysis for 9 paired HCC cells. (nos-nop.org)
  • MiR-31 was significantly down-regulated compared to related non-tumor cells. (nos-nop.org)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)
  • Cells from the bronchioloalveolar carcinoma cell line A549 were exposed to benzo(a)pyrene (B[a]P) for 24 weeks, and morphology, proliferative activity, and gene expression profiles were analyzed. (nih.gov)
  • Although no apparent morphologic changes were observed, the B[a]P-exposed A549 cells exhibited enhanced proliferative activity in 1% bovine serum that contained medium, and dramatic changes in expression levels were observed in a large number of genes. (nih.gov)
  • The enhancement of nuclear β-catenin looked as if it positively correlated with a significant increase in the basal expression of VEGF as well as increased expression of fibronectin and α-SMA. (molvis.org)
  • In conjunction with SB216763, coadministration of an HIF-1α translation inhibitor, but not an HIF-2α translation inhibitor, markedly suppressed the expression of fibronectin and α-SMA without affecting VEGF levels. (molvis.org)
  • MaR1 repressed TGF-B1-induced loss of E-cadherin expression and reduced matrix metalloprotease-2 activities and fibronectin production, while not impairing TGF-B1-mediated wound-healing capacity. (cdc.gov)
  • Gomez M, Cano A. Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells. (bdbiosciences.com)
  • It was demonstrated that expression of interleukin‑8 (IL‑8) mRNA was significantly upregulated in Gem‑R PaCa cells by cDNA microarray and RT‑qPCR analyses. (cancerindex.org)
  • To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
  • further, HMOX1 expression was strongly inhibited, suggesting that NR4A3 is an oxidative stress-responsive transcription factor that functions through HMOX1 expression in pancreatic islet ß-cells. (bvsalud.org)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • In addition, provided herein are conjugate vaccine carriers comprising engineered tetanus toxin variants and methods of using such conjugate vaccines to elicit T-cell dependent immune memory responses which can target a broad spectrum of microbial pathogens as a single vaccine. (justia.com)
  • Cell reports methods. (lu.se)
  • In this process, epithelial cells acquire fibroblast-like properties such as the functional loss of E-cadherin. (spandidos-publications.com)
  • To acquire correct information utilizing anti-VWF antibodies, it's needed to pay attention to the opportunity of cross-reactivity with fibronectin. (holliseden.com)
  • Besides the massive secretion of fibronectin (FN) that characterizes the early step of that process, alterations in the secretion of collagens also occurs as part of this wound healingresponse. (nih.gov)
  • In addition, immobilized 5H10-27 (MFR5) antibody reportedly costimulates the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. (bdbiosciences.com)
  • Cross-reactivity between the anti-VWF antibody and fibronectin was inhibited by pretreating the antibody with fibronectin-coated plates. (holliseden.com)
  • So as to generate an antibody directed enzyme prodrug remedy, right here we designed a chimeric protein by fusing the F8 antibody that acknowledges the EDA of fibronectin (expressed on the tumor neovasculature) and an developed variant of the ROS-generating enzyme D-amino acid oxidase (DAAO). (holliseden.com)
  • Management of fibrotic adjustments by means of the synergistic results of anti- fibronectin antibody and an RGDS-tagged type of the identical antibody . (holliseden.com)
  • Many microorganisms have mechanisms that impair antibody production by inducing suppressor cells, blocking antigen processing, and inhibiting lymphocyte mitogenesis. (msdmanuals.com)
  • Because remodeling of the actin cytoskeleton promotes morphological changes and cell migration during EMT and is also required for. (bioskinrevive.com)
  • insoluble cellular fibronectin is a major component of the extracellular matrix. (wikipedia.org)
  • One or both of the "extra" type III modules (EIIIA and EIIIB) may be present in cellular fibronectin, but they are never present in plasma fibronectin. (wikipedia.org)
  • It is present in most cellular fibronectin, but only one of the two subunits in a plasma fibronectin dimer contains a V-region sequence. (wikipedia.org)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • These changes resulted in increased actin stress fibers and enhanced migratory behavior of cells in two-dimensional culture. (dundee.ac.uk)
  • Actin filaments in epithelial cells are structured in cortical slim bundles. (bioskinrevive.com)
  • Fragmenting fibronectin further exposes its V-region, which contains the site for α4β1 integrin binding. (wikipedia.org)
  • These fragments of fibronectin are believed to enhance the binding of α4β1 integrin-expressing cells, allowing them to adhere to and forcefully contract the surrounding matrix. (wikipedia.org)
  • Right here, we examined whether or not function-blocking mAbs directed towards α5β1 can work together with the integrin after it varieties a posh with an RGD-containing fragment of fibronectin. (holliseden.com)
  • A brand new mannequin of the α5β1-fibronectin complicated helps our speculation that the epitopes of mAbs that fail to bind to the ligand-occupied state lie inside, or very near, the integrin-fibronectin interface. (holliseden.com)
  • Macrophages are essential to wound healing and perhaps are the most important cells in the early phase of wound healing. (medscape.com)
  • Neutrophils are the predominant cell type for the first 48 hours after injury but do not appear essential to the wound-healing process. (medscape.com)
  • Neutrophil-enriched populations showed the greatest activity in causing injury to epithelial cells and fibronectin in vitro. (hw.ac.uk)
  • 2009 ). These transcription elements travel EMT by repressing manifestation of epithelial genes and activating manifestation of mesenchymal genes. (bioskinrevive.com)
  • Western blot analysis was employed to detect the cytoplasmic and nuclear levels of β-catenin, as well as the total lysate content of fibronectin and α-SMA. (molvis.org)
  • Analysis of proteomic alteration was carried out using 2D-DIGE on both the total cell lysate and the hydrophobic proteomes. (dcu.ie)
  • These findings support the development of a potentially relevant therapeutic strategy to undermine the progression of normal cells to the mesenchymal phenotype in the naturally hypoxic lens without subverting cell viability. (molvis.org)
  • Fragmentation of fibronectin by proteases has been suggested to promote wound contraction, a critical step in wound healing. (wikipedia.org)
  • It had been discovered that miR-31 could suppress reporter gene activity in these cells, whereas mutants plasmids showed zero noticeable adjustments. (nos-nop.org)
  • Fibronectin plays a crucial role in wound healing. (wikipedia.org)
  • Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • Besides past service on the Councils of several professional societies, Dr. Yamada is currently an editor of The Journal of Cell Biology and serves other editorial roles at multiple journals. (nih.gov)
  • Dr. Yamada served on the NIH Cell Biology Study Section and currently serves on the NIH Committee on Scientific Conduct and Ethics, NIH Steering Committee Diversity Working Group, and various other NIH committees. (nih.gov)
  • 1 Wayne State University School of Medicine Department of Anatomy and Cell Biology USA Michigan, Detroit, MI 48201, USA. (nih.gov)
  • Also covered in detail was the basic cell biology of EMT and its role in cancer and fibrosis, as well as the identification of new markers to facilitate the observation of EMT in vivo. (rupress.org)
  • Patterned cell and matrix dynamics in branching morphogenesis. (nih.gov)
  • Fibronectin requirement in branching morphogenesis. (nih.gov)