Epithelial cell basal medium. Medical search. Frequent questions

To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-κB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-κB-dependent interleukin (IL)-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) along with the activation of NF-κB in non-CF and CF human bronchial gland epithelial cells. (ersjournals.com)
It was demonstrated that a relevant concentration of FP (10 −8 M) inhibited constitutive and P. aeruginosa LPS-induced IL-6 and IL-8 production of non-CF and CF bronchial epithelial cells. (ersjournals.com)
Interestingly, the expression of two IκB kinases (IKK)-α/β, the degradation of cytosolic IκB-β inhibitor and the NF-κB deoxyribonucleic acid binding activity were markedly reduced after FP treatment in both CF and non-CF bronchial epithelial cells. (ersjournals.com)
It was shown by the authors that fluticasone propionate exerts an anti-inflammatory effect by blocking a signal transduction leading to a reduced level of IκB-α/β kinases in bronchial epithelial cells. (ersjournals.com)
In particular the strong effect on the IκB-β kinase, which is known to be elevated in bronchial epithelial cells in cystic fibrosis patients, was observed. (ersjournals.com)
The present authors and previous studies, have previously demonstrated that epithelial expression of interleukin (IL)-8 is excessively elevated in the bronchial epithelial cells of human CF adults 6 . (ersjournals.com)
Primary normal human bronchial epithelial (NHBE) cells (Lonza, Walkersville, MD) from a 4-year-old healthy male donor were cultured in an air-liquid interface (ALI) system on Transwell inserts (Corning, NY). (cdc.gov)
Thus, the aim of this study was to investigate if aerosolized nZnO exposure leads to changes in the cellular composition of the airway epithelium, using air-liquid interface (ALI)-differentiated primary human bronchial epithelial cells. (cdc.gov)
Methods: Normal human bronchial epithelial cells (NHBEpC) (at ≥ 90% viability) were seeded on 12-well porous 0.4 µm- cell culture inserts in basal cell culture medium. (cdc.gov)
Media developed specifically for cultivation of bronchial epithelial cells in an air-liquid interface system. (lonza.com)
Bronchial epithelial cells (BEAS-2B cells and primary bronchial epithelial cells) were exposed to heat-inactivated Haemophilus influenzae , Pseudomonas aeruginosa or Streptococcus pneumoniae and subsequently infected with respiratory syncytial virus (RSV), type 2 human adenovirus or influenza B. Levels of pro-inflammatory cytokines, viral replication and expression of pattern recognition receptors were determined in culture supernatants and/or cell lysates. (biomedcentral.com)
Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
Primary normal human-derived bronchial epithelial cells (NHBEs) were exposed to ABS filament emissions in an ALI for 4hours. (cdc.gov)

Three-dimensional reconstruction of singly labeled intestinal cells demonstrated that the majority of the intestinal epithelial cells contact both the apical and basal surfaces. (jove.com)
Electron micrograph of the junctional complex of intestinal epithelial cells of the rat shows the apical-most zonula occludens (tight junction), the zonula adherens (medium junction) and the macula ad. (cellimagelibrary.org)

Inhaled fluticasone propionate (FP) is widely used to reduce pulmonary inflammation in chronic obstructive pulmonary disease, but the potential effects of FP on airway epithelial cells from patients with cystic fibrosis (CF) are unknown. (ersjournals.com)
In CF disease, a nonregulated inflammatory lung response occurs through exaggerated nuclear factor (NF)-κB activation and elevated pro-inflammatory cytokines production by airway epithelial cells. (ersjournals.com)
Cystic fibrosis (CF) is a lethal inherited disorder caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) in airway epithelial cells 1 . (ersjournals.com)
One of the major clinical manifestations of CFTR mutations is neutrophil dominated inflammation 2 , 3 , implying that defective CFTR affects the immune function of airway epithelial cells, in particular the production of nuclear factor (NF)-κB-dependent chemokines. (ersjournals.com)
We previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with corneal cell proliferation. (molvis.org)

Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
MEGM TM TM Mammary Epithelial Cell Growth Medium has been optimized for the growth of Mammary Epithelial cells in a serumfree environment. (legalcure.be)
Implications: Fibroblasts within mammary tumors can regulate the molecular phenotype and invasive behavior of breast cancer cells. (johnshopkins.edu)
Furthermore, certain microRNA species contained in exosomes from cultured breast cancer cells or ductal lavage samples differ from those secreted from normal mammary epithelial cells [ 14 ], suggesting the potential use of these exosome microRNAs as biomarkers for the detection of malignant breast disease. (biomedcentral.com)
MEBMTM mammary epithelial basal medium and supplementation (MEGMTM Mammary Epithelial Cell Growth Medium Kit) for MCF10-A cells, and DMEM F-12 medium (ATCC) supplemented with insulin-transferrin-selenium (ITS-G) for AML-12 cells were purchased from Lonza (Basel, Switzerland). (bioz.com)

Tracheal and lung explants were maintained on transwell inserts, and 1 mL of medium (as described for NHBE culture) was added to the basal chamber. (cdc.gov)
On the next day, the media from the apical and basal chambers was removed, and 1 mL complete ALI Medium containing necessary supplements was added to the basal chamber. (cdc.gov)

BEGM is optimized to provide the best growth for Normal Human Bronchial/Tracheal Epithelial Cells. (lonza.com)

FP induces apoptosis of eosinophils 18 and also reduces the production of several cytokines such as IL-1β, IL-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) by alveolar macrophages 19 and lymphocytes 20 , 21 . (ersjournals.com)
We investigated the potential of the ethanolic extract of saffron to induce antiproliferative and cytotoxic effects in cultured carcinomic human alveolar basal epithelial cells in comparison with non-malignant (L929) cells. (phcog.com)
For adenovirus-positive aerosol samples, 500 μL of sample was inoculated into adenocarcinomic human alveolar basal epithelial (A549) cells (ATCCR CCL 185™) with Dulbecco's Modified Eagle Medium (DMEM) 2% (v/v) Fetal Bovine Serum (FBS), and incubated at 37°C. After 72 hours, inoculated shell vials were observed for cytopathic effect (CPE) daily for ten days. (datexis.com)
Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)

Optimal differentiation was verified by qPCR and immunofluorescent (IF) staining of cell-type specific markers. (cdc.gov)
Results: qPCR analysis of the differentiation time-course shows a steady increase in markers of terminally differentiated cells, such as club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1). (cdc.gov)
The basal cell (stem/progenitor cell) marker (KRT5) shows an initial increase in expression, but did not vary much during differentiation. (cdc.gov)
Induced pluripotent stem (iPS) cells are a valuable resource for discovery of epigenetic changes critical to cell type-specific differentiation. (nih.gov)
DNA methylation profiling identified key pathways and regulators of prostatic differentiation including Wnt5a, BPM7, PTEN, and NKx3.1 using E-PZ-iPS-lke cells. (nih.gov)
These cells are pluripotent and capable of prostatic differentiation, and therefore provide a novel model for investigating epigenetic changes involved in prostate cell lineage specification. (nih.gov)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
CD24 is involved in regulating B cell apoptosis and in preventing terminal differentiation of B cells into antibody-secreting plasmablasts and plasma cells. (stemcell.com)
It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
JAG1-NOTCH signaling is required for mesenchymal stem cell (MSC) differentiation into cardiomyocytes and cranial neural crest (CNC) cells differentiation into osteoblasts, making it a regenerative candidate for clinical therapy to treat craniofacial bone loss and myocardial infarction. (bvsalud.org)
basal and luminal markers indicating prostate oncogenesis happens through disruption of an intermediate step in the prostate epithelial differentiation system. (crispr-reagents.com)
Thus we recognized a new epithelial cell differentiation switch including Myc Pten and ING4 which when disrupted prospects to prostate tumorigenesis. (crispr-reagents.com)
This is the 1st demonstration that transient ING4 manifestation is absolutely required for epithelial differentiation its manifestation is dependent on Myc and Pten and it is lost in Tacalcitol monohydrate the majority of human being prostate cancers. (crispr-reagents.com)
differentiation model in which AR-negative human being basal prostate epithelial cells can be differentiated into AR-positive and androgen-responsive post-mitotic secretory cells (12). (crispr-reagents.com)
Based on known prostate and epithelial differentiation markers and the demonstration that PSA can be secreted into the medium from your differentiated cells this model recapitulates the biology and physiology of the human being prostate Tacalcitol monohydrate gland AR (C-19) Nkx3.1 (H-50) and TMPRSS2 (H-50) were purchased from Santa Cruz. (crispr-reagents.com)
Reprogramming principal conjunctival cell cultures and following differentiation into RPE generated 14 iPSC-RPE lines from 10 No AMD donors (No AMD iPSC-RPE) and 24 lines from 16 AMD donors (AMD iPSC-RPE). (eagulf.net)

These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
MaSC/progenitor cells were identified based on their ability to retain bromodeoxyuridine for an extended period. (frontiersin.org)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Cells were harvested at 24 hours or 7 days post exposure, to investigate changes in (a) inflammation, (b) oxidative stress-related, and (c) cell-type specific markers using qPCR and/or IF staining. (cdc.gov)
Exposure to high dose of nZnO induced a significant decrease in expression of club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1) markers at mRNA levels. (cdc.gov)
Importantly, E-PZ-iPS-like cells re-expressed basal epithelial cell markers (CD44, p63, MAO-A) in response to prostate-specific medium in spheroid culture. (nih.gov)
Finally, when injected into mice, E-PZ-iPS-like cells expressed basal epithelial cell markers including CD44 and p63. (nih.gov)
Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
Collective invasion can be led by breast cancer cells expressing basal epithelial markers, typified by keratin-14 (KRT14). (johnshopkins.edu)
Outcomes evaluated were outgrowth sheet size from limbal explants, expression of stem/progenitor cell markers p63α, ABCG2 and CK15, and colony formation efficiency (CFE). (elsevierpure.com)
We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. (molcells.org)
At 24hours after the exposure, significant increases in the pro-inflammatory markers IL-12p70, IL-13, IL-15, IFN-, TNF-, IL-17A, VEGF, MCP-1, and MIP-1 were noted in the basolateral cell culture medium of ABS-exposed cells compared to non-exposed chamber control cells. (cdc.gov)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

In this study, we analyzed three bacterial toxins and five culture supernatants of selected bacteria with known toxicity as model agents exposed to the lung epithelial cell line NuLi-1. (biomedcentral.com)
We here investigated if pre-exposure to bacteria alters the response of lung epithelial cells to subsequent viral infection. (biomedcentral.com)

This flat tube of epithelium undergoes rapid proliferation, growing both in length and girth and undergoes dramatic rearrangements involving dynamic cell shape changes 1 . (jove.com)
Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex. (molvis.org)
Airway cell media are serum-free media that have been optimized for the proliferation of airway cells. (lonza.com)
miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
Innoprot MTT Cell Viability & Proliferation Assay allows simple, accurate and reliable counting of metabolically active cells, based on the conversion of. (biosave.com)
It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
When primary SSCs are derived from the testis and proliferated in vitro , mouse embryonic fibroblasts (MEFs) are the most commonly used feeder cells that play a role of such microniche by allowing secure attachment and proliferation of SSCs. (molcells.org)
MEFs used as feeder cells may also secrete certain components into the media that support SSC proliferation. (molcells.org)

In bronchial biopsies from 21 normal subjects, 11 subjects with chronic bronchitis, 9 subjects with mild asthma, and 31 subjects with severe asthma, we evaluated epithelial cell morphology: epithelial thickness, lamina reticularis (LR) thickness, and epithelial desquamation. (atsjournals.org)
Our study resulted in sequences of events marked by apoptosis, such as loss of cell viability, morphology changes that were evaluated by MTT assay and invert-microscope, respectively. (phcog.com)
Morphology of MCF7 cells treated with the ethanolic extract confirmed the MTT results. (phcog.com)
During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)
Content of the development factors, aswell as general cell morphology, uncovered no disease- or genotype-dependent distinctions. (eagulf.net)

Odontogenic keratocysts (OKCs) associated with NBCCS have occasionally been reported to transform into aggressive neoplasms such as ameloblastomas and squamous cell carcinoma . (medscape.com)
The most common epitheliomas are basal cell carcinoma and squamous cell carcinoma (skin cancers). (wikipedia.org)
Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. (medscape.com)

Coculture significantly increased KRT14 expression and invasion of organoids from both luminal and basal murine breast cancer models. (johnshopkins.edu)
C) The expression of ESRP1 in basal-like and Luminal A subtype were significantly higher than that of the Luminal B subtype of breast cancer, and the HR-sensitive subtype expressed higher level of ESRP1 than did triple-negative and Her-2 clinical subtypes (Gene Expression-Based Outcome for Breast Cancer Online, GOBO). (medscimonit.com)

Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. (thermofisher.com)
CTS™ Immune Cell Serum Replacement (SR) is designed to support expansion of in vitro cultured human immune cells such as T cells when added to a basal cell culture media such as CTS™ OpTmizer™ T cell Expansion SFM or CTS™ AIM-V™ SFM. (thermofisher.com)
Primary human corneal epithelial and trabecular meshwork cells (Science Cell, Carlsbad, CA) were cultured in manufacturer supplied media supplemented with FBS at 37°C in 5% CO2. (cdc.gov)
The adult human airway epithelium is comprised of four major cell types, basal, club, goblet, and ciliated cells. (cdc.gov)
Although iPS cells have been generated from other terminally differentiated cells, the reprogramming of normal adult human basal prostatic epithelial (E-PZ) cells to a pluripotent state has not been reported. (nih.gov)
The impact of real-world ambient air pollution exposure on human lung and olfactory cells grown at the air-liquid interface. (cas.cz)
Since olfactory mucosa is considered one of the most feasible routes of air pollutants entry into the brain [27] , we will further study the effects of air pollution exposure in human olfactory mucosa cells (hOM) obtained from 8 healthy subjects and 8 Alzheimer's disease (AD) patients (provided by a collaborative institution - Prof. Katja Kanninen, University of Eastern Finland). (cas.cz)
Human bronchial/tracheal epithelial cells isolated from the epithelial cells that line the airway above the bifurcation of the lungs. (lonza.com)
Melanocyte Growth Medium M2 is a serum-free and PMA (Phorbol Myristate Acetate)-free Medium that was developed for the optimized in vitro cultivation of human mel. (scientificlabs.co.uk)
It is also suitable for human pigment epithelial cells, neuronal cells, and melanoma cells. (scientificlabs.co.uk)
All lots of PromoCell Melanocyte Growth Medium M2 are subjected to comprehensive quality control tests using primary human melanocytes. (scientificlabs.co.uk)
Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
Human Liver Sinusoidal Endothelial cells were isolated from normal human liver tissue. (neuromics.com)
Human Liver Sinusoidal Endothelial Cells are negative for HIV-1, HBV, HCV, and mycoplasma. (neuromics.com)
Using this CF defect model in vivo, we delivered JAG1 with pediatric human bone-derived osteoblast-like (HBO) cells to demonstrate the osteo-inductive properties of JAG1 in human cells and in vitro we utilized the HBO cells to identify the downstream non-canonical JAG1 signaling intermediates as effective bone regenerative treatments. (bvsalud.org)
Exosomes were collected from the conditioned media of human breast cancer cell lines, mouse plasma of patient-derived orthotopic xenograft models (PDX), and human plasma samples. (biomedcentral.com)
Here we comparatively investigated the optimized conditions and the efficiency of limbal epithelial sheet growth in three media conditions as well as with substrate free (transwell), human amniotic membrane (HAM) sutured onto transwell inserts (HAMTW), and HAM slide scaffold (HAMS). (elsevierpure.com)
Limbal epithelial sheets with 5% human AB serum showed the greatest increase in ABCG2 efflux activity (JC1 low ), p63α expression, and CFE compared in both conditions without HAM and with HAM, respectively. (elsevierpure.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

Cultivation of limbal epithelial sheet on HAMS with xeno-free medium enhances the growth and stemness of limbal epithelial sheets. (elsevierpure.com)

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that help you meet your research needs and budget requirements. (thermofisher.com)
The ability to observe the epithelium three-dimensionally was seminal to demonstrating this point and redefining epithelial morphogenesis in the fetal intestine. (jove.com)
Importin 13 (IPO13), a member of the importin-β superfamily, was originally identified in the fetal rat lung and differentially expressed in cells of epithelial and mesenchymal origin. (molvis.org)
Fetal bovine turbinate cells (courtesy of Swedish Veterinary Institute) propagated in Eagle's minimal essential medium (BioWhittaker, Belgium) in 96 well plates were incubated with 50 μl filtered swab samples for 30 min. (datexis.com)

In the breast, intraductal papilloma (IDP) is a benign proliferative lesion that consists of a branching fibrovascular core with overlying epithelial and myoepithelial layers. (medscape.com)

The cells will be grown at the air-liquid interface (ALI = cells are grown on a membrane with their basal surfaces in contact with media, and the top of the cellular layer is exposed to the air) in our exposure system in the field conditions of localities differing in air pollution levels. (cas.cz)
It creates a positive hydrostatic pressure on the rigid cell wall by the cell membrane. (samacheer-kalvi.com)
Hence forth the pressure exerted by the ccli membrane towards the cell wall is Turgor Pressure (TP). (samacheer-kalvi.com)
Cells of hematopoietic origin are individual cells, mostly round in shape, and have a distinct cytoplasmic membrane. (vin.com)

It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use. (thermofisher.com)

Transplantation of cultivated limbal epithelial transplantation has been proven to restore the corneal surface in limbal stem cell deficiency (LSCD). (elsevierpure.com)

These cultures contain fibroblasts/stromal-like cells, globose basal cells and myofibroblasts-like cells obtained from a biopsy from the nasal septum and further processed as previously described [28] . (cas.cz)
We then developed a novel coculture model of tumor organoids with autologous stromal cells. (johnshopkins.edu)
However, stromal cell conditioned medium induced invasion but not KRT14 expression. (johnshopkins.edu)
Cancer cells released TGFb and that signaling pathway was required for stromal cell-induced invasion and KRT14 expression. (johnshopkins.edu)
Mechanistically, TGFb induced NOX4 expression in stromal cells and NOX4 inhibition reduced invasion and KRT14 expression. (johnshopkins.edu)
In summary, we developed a novel coculture model and revealed dynamic molecular interactions between stromal cells and cancer cells that regulate both basal gene expression and invasive behavior. (johnshopkins.edu)
BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) are polymorphic, adherent cells with the capability to stimulate tissue regeneration and modulate immunity. (bvsalud.org)
We identified a significant induction of epithelial alarmin (thymic stromal lymphopoietin-TSLP)-dependent interleukin-13 pathway after co-exposure, associated with increased mucin and interferon gene expression. (cdc.gov)

Basal surfaces were cultured in BEBM medium (Lonza) supplemented with SingleQuot growth factors (Lonza). (cdc.gov)
The apical surfaces of NHBEs or explants were washed with PBS and equilibrated at 37°C for 30 min with infection medium (BEBM supplemented with 0.5% BSA). (cdc.gov)
Direct immunofluorescence microscopy performed on epithelial biopsy specimen obtained from a patient with pemphigus vulgaris detects immunoglobulin G deposits at the epithelial cell surfaces. (medscape.com)

Benign epithelial cells do also have a round nucleus. (vin.com)
HGPIN is characterized by architecturally benign prostatic acini and ducts lined with atypical cells whose morphologic, histochemical, immunohistochemical, and genetic changes are similar to those of prostate cancer. (medscape.com)

The presence of all four major cell types at protein level was confirmed by IF staining of the fully differentiated airway epithelium. (cdc.gov)
Since these cells reside at the bottom of the pseudostratified airway epithelium, it's possible that they were not directly impacted by nZnO exposure, at the doses/time points used. (cdc.gov)
The mouse small intestine is formed at embryonic day 10 when a sheet of endoderm closes and seals to form a tube of epithelium surrounded by mesenchymal cells 3 . (jove.com)
In the proximal small intestine at embryonic day 14.5, condensations (clusters) of Hedgehog- and PDGF-responsive cells begin to form adjacent to the epithelium 2,5 . (jove.com)
The model represents a fully differentiated and functional respiratory epithelium consisting of basal, goblet and ciliated cells [26] . (cas.cz)
IPO13 activity was significantly increased in the basal layer of the epithelium of the pterygium. (molvis.org)
cells are the most sensitive cell type of this epithelium. (cdc.gov)

We find that when aggresomes are present, neuronal cells are unable to organise their microtubule network. (biorxiv.org)

Each intestinal villus is composed of two main tissue compartments: an epithelial surface layer and a mesenchymal core. (jove.com)
Looking for reliable and cost effective Cell and Tissue Culture products for your research? (biosave.com)
Recent efforts have been made to develop plant-based cell culture media to replace media based on materials derived from animals or bacteria, such as E. coli, bovine (casein), or porcine brain and heart tissue, which are known to contain trace amounts of contaminants, such as endotoxins, that adversely affect stem cell and other cell cultures. (biosave.com)
Tissue culture media must support living tissues, for example rabbit retinae, maintaining their metabolic and electrical responses as long as possible for the duration of a study. (biosave.com)
Cells of this type of tissue have the tendency to be connected to each other. (vin.com)
Within this type are included the cells arising from connective tissue, muscle, cartilage, and bone. (vin.com)
Smears of this type of tissue are thus also characterized by a low cellularity and the cells are mostly separate from each other, although groups of cells can also be found. (vin.com)

Recently, an in vitro study on the expression of ΔF508 CFTR (CFTR which contains a deletion of phenylalanine at position 508) in the ovary cells of Chinese hamsters, which do not express CFTR, revealed a seven-fold increase in the activation of NF-κB compared to the wild-type CFTR or the G551D mutant. (ersjournals.com)
E-PZ-iPS-like cells demonstrated pluripotency by differentiating into ectodermal, mesodermal, and endodermal cells in vitro, although lack of teratoma formation in vivo and incomplete demethylation of pluripotency genes suggested only partial reprogramming. (nih.gov)
SSCs can be isolated from the testis and cultured in vitro for longterm periods in the presence of feeder cells (often mouse embryonic fibroblasts). (molcells.org)
Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost- effective, and preserves self-renewability of SSCs. (molcells.org)
Established SSCs are useful stem cell lines that allow not only to study basic reproductive biology but also to develop an in vitro model for applications in assisted reproductive medicine. (molcells.org)

Infection with influenza B on the other hand did not affect cytokine production in BEAS-2B cells exposed to the different bacterial strains. (biomedcentral.com)

CD24 is differentially expressed during T and B cell development, and is not found on the majority of mature peripheral T cells or plasma cells. (stemcell.com)

Students can Download Tamil Nadu 11th Biology Model Question Paper 3 English Medium Pdf, Tamil Nadu 11th Biology Model Question Papers helps you to revise the complete Tamilnadu State Board New Syllabus, helps students complete homework assignments and to score high marks in board exams. (samacheer-kalvi.com)

OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion. (thermofisher.com)
During virus infections, cells were switched to serum free-medium supplemented with 1% bovine serum albumin and 0.5 g/mL TPCK treated trypsin. (cdc.gov)
Pterygium epithelial cells (PECs) were cultured in keratinocyte serum-free defined medium to examine the expression of IPO13 and K17. (molvis.org)

Increasing evidence suggests that respiratory epithelial cells with dysfunctional CFTR have exaggerated activation of NF-κB/inhibitor of NF-κB complex associated with upregulated expression of chemokines 4 , 5 . (ersjournals.com)
The effects of corticosteroids are predominantly mediated by the inhibition of NF-κB deoxyribonucleic acid (DNA)-binding activity, which controls genes encoding inflammatory cytokines, chemokines and adhesion molecules in respiratory epithelial cells 10 - 13 . (ersjournals.com)

As mesenchymal clusters form, the epithelial cells closest to the clusters begin to withdraw from the cell cycle, while the other epithelial cells continue to proliferate. (jove.com)
Those cells directly above the mesenchymal cluster that have withdrawn from the cell cycle begin to change shape as the emerging villus buckles into the lumen. (jove.com)
Bone marrow-derived mesenchymal stem cell-based treatments, which do not have the side effects of BMP2, are not currently FDA approved, and are time and resource intensive. (bvsalud.org)

Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
Current bone regenerative approaches have consistently relied on BMP-2 application with or without addition of stem cells. (bvsalud.org)
PREPARING TOOTH-LIKE STRUCTURE USING STEM CELL - The usage of a stem cell in preparation of a tooth-like structure is provided. (patentsencyclopedia.com)
Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. (molcells.org)
Spermatogonial stem cells (SSCs), also called germline stem cells, are unipotent precursor cells that self-renew and contribute to spermatogenesis in the testis. (molcells.org)

Each toxin resulted in a different but specific impedance profile over time according to their mode of action, whereas viability assay showed the metabolic activity of the cells at a chosen time point without revealing any information on their mode of action. (biomedcentral.com)
The established assays revealed the concentration dependent onset and intensity of bacterial cytotoxicity and the viability of the cells at 24 h and 48 h exposure. (biomedcentral.com)
A non-nutritional salt-based medium can be used to maintain the viability of microorganisms for transport, without significant increase in growth. (biosave.com)
The results showed that the ethanolic extract of saffron decreased cell viability in malignant cells as a concentration and time-dependent manner. (phcog.com)
However, the extract at different concentrations could not significantly decrease the cell viability in L929 cells. (phcog.com)
A - F ). Dose-dependent effect of ethanolic extract of passion fruit seeds on the viability of BEAS-2B, AML-12, and MCF-10A cell lines after 24 h of incubation. (bioz.com)

To confirm the importance of M2 splicing in the replication of H1N1 and H3N2, we treated infected cells with splicing inhibitor herboxidiene and analyzed the viral growth using plaque assay. (biomedcentral.com)
There was a greater level of apoptotic activity in the airway biopsy in subjects with severe asthma as compared with the normal subjects using the TUNEL assay (p = 0.002), suggesting increased cell death. (atsjournals.org)

Both cells were cultured in Dulbecco's modified Eagle's medium and treated with the ethanolic extract of saffron at various concentrations for two consecutive days. (phcog.com)

In parallel, the expression of basal cell marker(KRT5) was partially modulated by nZnO exposure. (cdc.gov)
Pre-exposure of epithelial cells to bacteria alters the response to subsequent viral infection depending on the types of pathogen involved. (biomedcentral.com)
A - F ). The relative amount of reactive oxygen species (ROS) assessed on BEAS-2B, AML-12, and MCF-10A cell lines after exposure to either carcinogen alone or with pretreatment of PESE or PIC. (bioz.com)

The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

The cells were observed for cytopathic effect (CPE) and infection visualized by direct immunofluorescence test using FITC Moab a-BRSV (Bio-X Diagnostics, Rochefort, Belgium). (datexis.com)

How CFTR dysfunction in CF airway cells contributes to the activation and nuclear localisation of NF-κB is still unclear. (ersjournals.com)
Pro-inflammatory 5-LOX metabolites were selectively increased in post COVID-19 MDM, suggesting that SARS-CoV-2 infection drives a pro-inflammatory eicosanoid reprogramming that contributes to long-term alterations in innate immune cell function. (nature.com)

ENDO-Growth Medium (MED001) is recommended for culture. (neuromics.com)

These E-PZ-iPS-like cells with normal karyotype gained expression of pluripotent genes typical of iPS cells (Tra-1-81, SSEA-3, Nanog, Sox2, and Oct4) and lost gene expression characteristic of basal prostatic epithelial cells (CK5, CK14, and p63). (nih.gov)

The stochastic inheritance of chromosome fragments by both newly formed daughter cells could in part contribute to the alternating DNA copy-number states that are characteristic of chromothripsis 2 . (nature.com)
The cells can have characteristic morphologic forms, of which the spindle-shaped cell is the best known. (vin.com)

A novel HEK293/Cytc-tGFP cell line has been developed through stable transfection for monitoring the cellular apoptosis level trhougth the Cytochrome c. (biosave.com)

This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)

Since dopaminergic neurons are polarised, ensuring correct localisation of organelles and the effective intracellular transport of neurotransmitter vesicles, loss of centrosome activity could contribute to loss of dopaminergic function and neuronal cell death in Parkinson's disease. (biorxiv.org)
In addition, we provide evidence that many cell types, including dopaminergic neurons, cannot form cilia when aggresomes are present, which would affect their ability to receive extracellular signals. (biorxiv.org)

Aml 12 Cells, supplied by Lonza, used in various techniques. (bioz.com)

After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. (scientificlabs.co.uk)
Product Overview MEGM TM Bulletkit TM, is also sold as separate basal medium and supplements. (legalcure.be)

We previously demonstrated that JAG1 induces murine cranial neural crest (CNC) cells towards osteoblast commitment via a NOTCH non-canonical pathway involving JAK2-STAT5 (1) and that JAG1 delivery with CNC cells elicits bone regeneration in vivo. (bvsalud.org)

Transitional epithelial cells of the bladder. (cellimagelibrary.org)

In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma). (scientificlabs.co.uk)

Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. (molcells.org)

Androgen induced expression of androgen receptor (AR), and co-culture with rat urogenital sinus further induced expression of prostate-specific antigen, a hallmark of secretory cells, suggesting that E-PZ-iPS-like cells have the capacity to differentiate into prostatic basal and secretory epithelial cells. (nih.gov)

We confirmed concentration dependent cytotoxic effects of the selected toxins in NuLi-1 cells over a period of up to 48 h. (biomedcentral.com)

The toxin forms transmembrane pores, which leads to the collapse of the cells and epithelial cells can no longer exercise their barrier function [ 6 ]. (biomedcentral.com)

Cellular toxicity was quantified by measuring lactate dehydrogenase (LDH) activity in the 'apical wash' and basolateral chamber media. (cdc.gov)
Cellular toxicity analysis showed that the higher dose of nZnO (Mean +/- SD) 4.25 +/- 0.41 µg/cm2 induced a significant increase in LDH activity (in apical wash and basolateral chamber media) at both 24 hours and 7 days' time points, as compared to the low dose nZnO (0.514 +/- 0.09 µg/cm2), and vehicle control. (cdc.gov)

fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

Donor Demographics for iPSC-RPE The somatic cell supply for the iPSC-RPE found in this research had been epithelial cells in the conjunctiva of Capromorelin adult individual donor eye. (eagulf.net)

JAG1 is a ligand that activates the NOTCH signaling pathway which plays a crucial role in determining cell fate behavior through cell-to-cell signaling. (bvsalud.org)

Anatomy70

Organisms14

Diseases9

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes54

Disciplines and Occupations2

Information Science6

Health Care1

Bronchial epithelial13

Intestinal epithelial cells2

Airway epithelial cells5

Mammary9

Added to the basal chamber2

Normal human bronchial1

Alveolar4

Differentiation16

Progenitor cells4

Markers12

Lung epithelial2

Proliferation9

Morphology5

Squamous cell carc3

Luminal2

Human20

Cultivation1

Fetal4

Myoepithelial1

Membrane4

Xeno-free medium1

Corneal1

Stromal8

Surfaces3

Benign2

Epithelium7

Neuronal cells1

Tissue7

Vitro5

Cytokine production1

Differentially expressed1

Biology1

Serum-free3

Respiratory2

Mesenchymal3

Stem5

Viability6

Assay2

Ethanolic extract1

Exposure3

Marker1

Immunofluorescence1

Contributes2

Growth Medium1

Gene expression1

Characteristic2

Apoptosis1

Lesions1

Neurons2

Lonza1

Supplements2

Murine1

Transitional1

Bacteria1

Transplantation1

Secretory cells1

Cytotoxic effects1

Transmembrane1

Toxicity2

Fluorescence-activated cell1

Adult1

Pathway1