Enzymes protein domain carrier proteins. Medical search. Frequent questions

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)

3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-3-O-((3R)- hydroxytetradecanoyl)-alpha-D-glucosamine = UDP-2-N,3-O-bis((3R)-3- hydroxytetradecanoyl)-alpha-D-glucosamine + a holo-[acyl-carrier- protein]. (cathdb.info)
LpxD from Escherichia coli prefers (R,S)-3-hydroxymyristoyl-[acyl- carrier protein] over (R,S)-3-hydroxypalmitoyl-[acyl-carrier protein]. (cathdb.info)
The α-subunits of the oxaloacetate and methylmalonyl-CoA decarboxylases are homologous to many biotin-containing enzymes including (1) pyruvate carboxylases, (2) homocitrate synthases, (3) biotin carboxyl carrier proteins, (4) isopropylmalate synthases and (5) acyl-CoA carboxylase. (tcdb.org)
Then, Acetyl-CoA and malonyl-CoA are coupled to the acyl-carrier protein domain of the rate-limiting enzyme fatty acid synthase (FASN) [ 4 ] . (encyclopedia.pub)
The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)
The human 4'-phosphopantetheine transferase is also capable of phosphopantetheinylation of peptidyl carrier and acyl carrier proteins from prokaryotes. (rhea-db.org)
Tries to crystallize the YchM STAS area resulted in spontaneous co-crystallization with acyl carrier proteins (ACP) liganded with malonyl coA (Babu et al. (exposed-skin-care.net)
Enoyl-(Acyl carrier protein) reductase [Interproscan]. (ntu.edu.sg)

The remarkable diversity of natural products (NPs) results from a similarly diverse pool of biosynthetic enzymes [ 2 ]. (biomedcentral.com)
to a membrane-scaffolded organic of fatty acidity biosynthetic enzymes (Babu et al. (exposed-skin-care.net)

The enzyme is biotin-dependent. (wikipedia.org)
The alpha subunit of PCC contains the biotin carboxylase (BC) and biotin carboxyl carrier protein (BCCP) domains. (wikipedia.org)
The native enzyme to biotin ratio has been determined to be one mole native enzyme to 4 moles biotin. (wikipedia.org)
All four enzyme porters are biotin-containing multisubunit enzymes. (tcdb.org)
The enzyme transfers CO 2 from glutaconyl-CoA to a biotin carrier protein (the γ-subunit) that is subsequently decarboxylated by the carboxybiotin decarboxylation site within the Na + pumping beta subunit (Gcdβ). (tcdb.org)
PC is a biotin-dependent mitochondrial enzyme that plays an important role in energy production and anaplerotic pathways. (medscape.com)
Some cases of partial enzyme deficiency respond to the administration of excess biotin. (medscape.com)
The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all biotin-dependent enzymes, such as acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, geranyl-CoA carboxylase, oxaloacetate decarboxylase, methylmalonyl-CoA decarboxylase, transcarboxylase and urea amidolyase. (umbc.edu)

Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)
This distribution comprises a small fraction of protein-specific hydration sites, where water rotation is strongly retarded, and a dominant fraction of generic hydration sites, where water rotation is as fast as in the hydration shells of small peptides. (lu.se)

We have structurally characterized the final peptidyl carrier protein domain of the teicoplanin non-ribosomal peptide synthetase machinery: this domain is believed to mediate the interactions with tailoring Oxy enzymes in addition to its function as a shuttle for intermediates between multiple non-ribosomal peptide synthetase domains. (rcsb.org)

COPI is recruited to the membrane primarily through binding to Arf GTPases, upon which it undergoes assembly to form coated transport intermediates responsible for trafficking numerous proteins, including Golgi-resident enzymes. (univ-lille.fr)
Built YchM deletion exhibited artificial lethality with multiple fatty acidity biosynthesis genes, as well as the YchM polypeptide bodily interacted numerous proteins from the fatty acidity biosynthetic pathway. (exposed-skin-care.net)

protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)

While we found non-cyanobacterial homologs of these enzymes to be rare, we identified a large number of genes encoding CylC-like enzymes in publicly available cyanobacterial genomes and in our in-house culture collection of cyanobacteria. (biomedcentral.com)
This domain occurred 8 times on human genes ( 20 proteins). (umbc.edu)
Epigenetic components (for example, Polycomb PRC1/2 and Trithorax group proteins) maintain the 'off' states of certain genes and the 'on' states of others, in a cell-type- and time-specific manner (the bottom panels show three genes, depicted schematically as chromatinized templates, in which transcription is triggered by specific transcription factors and silent or active states are maintained by PRC1/2 or Trithorax proteins, respectively). (nature.com)
Certainly, STAS domain-encoding SulP genes have already been found as the different parts of antibiotic operons, like the BLM bleomycin biosynthesis operon of (Du et al. (exposed-skin-care.net)
In most gram-positive bacteria, this operon consists of five genes dltXABCD but the involvement of the first ORF ( dltX ) encoding a small protein of unknown function, has never been investigated. (frontiersin.org)
In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)

When multiple copies of a polypeptide encoded by a gene form an aggregate, this protein structure is referred to as a multimer. (wikipedia.org)
At Biogen, we are developing a diverse portfolio consisting of small molecules, proteins, antisense oligonucleotides and gene therapy to target many neurodegenerative diseases. (chi-peptalk.com)
The three proteins share between 21 and 29% identity (37C50% similarity) with LCP proteins from (MsrR, SA0908, and SA2103), (CpsA2), and (TagT, TagU, and TagV) (Fig. 2maps to an AG biosynthetic gene cluster adjacent to ((genus and other AG-producing Actinobacteria, including species, and is a pseudogene in LCP protein candidates. (sicollaborative.org)

Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. (bio-techne.com)
Anaplastic lymphoma kinase (ALK) protein may be detected in most cases (60-70%) of systemic ALCL by immunohistochemistry. (medscape.com)
In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)

MftC is a radical S-adenosylmethionine (SAM) enzyme that may function to modify mycofactocin, a conserved polypeptide that might serve as an electron carrier. (nih.gov)
However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). (novusbio.com)

Loss of GORAB causes impairment of COPI-mediated retrieval of trans-Golgi enzymes, resulting in a deficit in glycosylation of secretory cargo proteins. (univ-lille.fr)

This group is composed of Mycobacterium tuberculosis putative mycofactocin radical SAM maturase MftC and similar proteins. (nih.gov)

protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74850","yeaD","Escherichia coli","D-hexose-6-phosphate epimerase-like protein [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)

To cause sensitization, a chemical must bind to macromolecules (proteins) in the skin. (cdc.gov)

Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)

The study of CylC-like enzymes will provide insights into substrate scope, mechanism and catalytic partners, and will also enable engineering these biocatalysts for similar or additional C-H activating functions. (biomedcentral.com)
Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. (nih.gov)
3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)
It has an N-terminal nucleotide-binding domain, which contains ATPase activity, and a C-terminal substrate-binding domain (1). (novusbio.com)

Despite intensive efforts to identify the ligase(s) responsible for the covalent attachment of AG to PG, no enzymes had yet been categorically implicated in the ligation process. (sicollaborative.org)

The β-subunits possess 9 transmembrane α-helical spanners (TMSs), and the protein may dip into the membrane twice between TMSs III and IV. (tcdb.org)

Defects in MFE2 are a cause of D-bifunctional protein deficiency (DBPD) [ MIM:261515 ]. (mitosciences.com)
In certain metabolic disorders ( ADA and PNP deficiency) enzyme substitution therapy can be applied. (lu.se)

The role of Albumin in Metabolic ActivitySerum albumin is a single-chain protein synthesized in and secreted from liver cells. (artscolumbia.org)

Mouse CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 2, 4). (bio-techne.com)

It is acted upon by a racemase, yielding (R)-methylmalonyl-CoA, which is then converted into succinyl-CoA by methylmalonyl-CoA mutase (one of the few metabolic enzymes which requires vitamin B12 as a cofactor). (wikipedia.org)

A domain known as the BT domain is also located on the alpha subunit and is essential for interactions with the beta subunit. (wikipedia.org)

2010). NMR answer structures have already been resolved for the STAS BIIE 0246 supplier domain name of putative sulfate transporter Rv1739c from (Sharma et al. (exposed-skin-care.net)
Open up in another window Physique 2 Structural topology style of SLC26 polypeptides displaying the brief cytoplasmic N-terminal area accompanied by a transmembrane domain name with 12 putative membrane-spanning -helices, as well as the C-terminal cytoplasmic area, largely composed of the STAS domain name. (exposed-skin-care.net)

The biosynthesis of the glycopeptide antibiotics, of which teicoplanin and vancomycin are representative members, relies on the combination of non-ribosomal peptide synthesis and modification of the peptide by cytochrome P450 (Oxy) enzymes while the peptide remains bound to the peptide synthesis machinery. (rcsb.org)
The structures exhibit the same general fold as the majority of known carrier protein structures, in spite of the complex biosynthetic role that PCP domains from the final non-ribosomal peptide synthetase module must play in glycopeptide antibiotic biosynthesis. (rcsb.org)
The enzyme catalyzes a step of lipid A biosynthesis. (cathdb.info)

Our results highlight the participation of two of these proteins in the phosphotransferase reaction leading to the ligation of AG and PG, a crucial step in the assembly of the entire complex cell wall of H37Rv yielded three candidates: Rv0822c, Rv3267 (herein renamed CpsA1), and Rv3484 (herein renamed CpsA2). (sicollaborative.org)

Transport by partitioning is distinct from both bulk flow and receptor-mediated transport, because differently from bulk flow, cargo can be concentrated in transport carriers, yet the concentrating process does not depend on specific cargo receptors ( Fig. 1 and Box 1 ). (biologists.com)
ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). (rndsystems.com)
Proteins that do not bind the receptor are directed to lysosomal degradation. (nature.com)

In the interaction with proteins, lead binds with virtually every available functional group, including sulfhydryl, amine, phosphate, and carboxyl groups, with sulfhydryl having the highest affinity. (cdc.gov)

In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)
The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
COPI is a key mediator of protein trafficking within the secretory pathway. (univ-lille.fr)

The BC and CT sites are approximately 55 Å apart, indicative of the entire BCCP domain translocating during catalysis of the carboxylation of propionyl-CoA. (wikipedia.org)

These structures thus support the hypothesis that it is subtle rearrangements, rather than dramatic conformational changes, which govern carrier protein interactions and selectivity during non-ribosomal peptide synthesis. (rcsb.org)
No pairwise interactions are available for this conserved domain. (umbc.edu)
This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
GORAB forms stable domains at the trans-Golgi that, via interactions with the COPI-binding protein Scyl1, promote COPI recruitment to these domains. (univ-lille.fr)
Pathogenic GORAB mutations perturb Scyl1 binding or GORAB assembly into domains, indicating the importance of these interactions. (univ-lille.fr)
To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)

For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
The formation of AG is set up in the cytoplasm on the decaprenyl phosphate (Dec-P) carrier lipid with formation from the linker device accompanied by the addition of Galand Araresidues (10,C12). (sicollaborative.org)

The N-terminal domain binds the glutaconyl-CoA, and the C-terminal domain binds the biotinyl lysine moiety. (tcdb.org)

Search proteins in UniProtKB for this molecule. (rhea-db.org)

Along with the deliver of drugs, it is essential to maintain normal cellular activity, which is done through by series of serum enzymes such as esterase. (artscolumbia.org)
It has similarity to the SABP2 methyl salicylate esterase from tobacco but no enzymatic activity has been identified for this protein. (or.jp)

It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. (bio-techne.com)

These enzymes are found in a variety of biosynthetic contexts, which include fatty-acid activating enzymes, type I or type III polyketide synthases, dialkylresorcinol-generating enzymes, monooxygenases or Rieske proteins. (biomedcentral.com)
In mammals, FA elongation depends on a set of enzymes termed elongation of very long-chain fatty acid enzymes (ELOVLs) [ 7 ] . (encyclopedia.pub)
1 Indeed, the concept of using molecules to manipulate other molecules in robotic fashion is an intriguing one that has some precedence in biology: for example, in metazoan fatty acid synthase a growing fatty acid chain, tethered to an embedded carrier protein, is passed between enzyme domains in the protein superstructure in a manner reminiscent of the way a robotic arm manipulates objects on a factory assembly line (Figure 1). (websitehome.co.uk)

The selectivity with which halogenating enzymes act on their substrates renders halogenases interesting targets for biocatalyst development. (biomedcentral.com)

This group appears to contain one auxillary Fe-S cluster that is similar to the second auxillary 4Fe-4S cluster (AuxII) of Clostridium perfringens anaerobic sulfatase-maturating enzyme (anSME). (nih.gov)

The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. (molvis.org)
Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. (novusbio.com)

The protein is around 70kDa and made up of 3 domains, a short chain alcohol dehydrogenase reductase, a central hydratase domain and a C terminal sterol carrier protein 2 domain. (mitosciences.com)

Distinct enzymes catalyze decarboxylation of (1) oxaloacetate, (2) methylmalonyl-CoA, (3) glutaconyl-CoA and (4) malonate. (tcdb.org)

Our results therefore identify GORAB as a COPI scaffolding factor, and support the view that defective protein glycosylation is a major disease mechanism in gerodermia osteodysplastica. (univ-lille.fr)
LCP-like proteins have RU.521 (RU320521) further been involved in the transfer of capsular polysaccharides to PG and the glycosylation of cell wall-associated proteins in a variety of CD47 other Gram-positive organisms (22,C29). (sicollaborative.org)

The STAS domain name of Rv1739c binds and adjustments conformation in response to guanine nucleotides (Sharma et al. (exposed-skin-care.net)
Lead also binds to metallothionein, a sulfhydryl-rich protein, but does not appear to displace cadmium or zinc. (cdc.gov)

The resulting sequence data holds immense opportunities for the discovery of new microbial enzymes and their associated NPs [ 4 ]. (biomedcentral.com)

Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)

A more bodies specific for the relatively conserved ectodomain of drastic antigenic change, termed antigenic shift, occurs if a matrix protein 2 and the intersubunit region of hemagglu- new HA subtype is introduced into the pool of human virus tinin. (cdc.gov)

Protein identity confirmed by mass spectrometry. (rndsystems.com)

Transport proteins that carry specific substances in the blood or across cell membranes. (reference.md)

CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)
Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12). (novusbio.com)

Nevertheless, they play crucial roles in cell morphology and division and are important for many cell envelope-dependent processes such as the activity of autolytic enzymes, the binding of divalent cations, and susceptibility to innate host defenses ( Weidenmaier and Peschel, 2008 ). (frontiersin.org)

It has been discovered that albumin not only acts as a carrier protein for drugs, but also participates in catabolic activity such as hydrolysis. (artscolumbia.org)
each of these domains have a subdomain A and subdomain B. Researchers has found these subdomains of the albumin molecule are essential for binding and transportation of antibiotics as well as other pharmaceuticals. (artscolumbia.org)
The domain II and III of albumin contain two primary drug binding sites, known as Sudlow's site I and site II (Alekseev & Rebane, 2012). (artscolumbia.org)

The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)

The active site of the dimeric enzyme lies at the interface between the two monomers. (tcdb.org)

Probable molybdopterin binding domain [Interproscan]. (ntu.edu.sg)
Molydopterin dinucleotide binding domain, Molybdopterin oxidoreductase Fe4S4 domain [Interproscan]. (ntu.edu.sg)
4Fe-4S binding domain, 4Fe-4S dicluster domain [Interproscan]. (ntu.edu.sg)
Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1-3). (bio-techne.com)
He also showed that double-stranded (ds)RNA- and ZDNA binding proteins had a role in poxvirus pathogenesis. (cdc.gov)
This perspective focuses on two areas that have yielded new useful information during the last 20 years: (i) structure-activity relationship (SAR) studies of contact allergy based on the concept of hapten-protein binding and (ii) mechanistic investigations regarding activation of nonsensitizing compounds to contact allergens by air oxidation or skin metabolism. (cdc.gov)

its ability to exchange electrons as it cycles between cuprous and cupric states has been harnessed by enzymes that catalyze a wide variety of biochemical processes [ 1 , 2 ]. (biorxiv.org)

2 However, up to now there have been no small-molecule machines (as opposed to proteins or DNA) that can transport molecular fragments in a similar manner. (websitehome.co.uk)
However, there are significant challenges due to inherent molecular complexity of proteins. (chi-peptalk.com)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)

Anatomy13

Organisms13

Diseases2

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes75

Disciplines and Occupations3

Information Science11

Health Care3

Recombinant proteins1

Acyl8

Biosynthetic enzymes2

Biotin8

Peptides2

Peptidyl carrier1

Numerous proteins2

Pyruvate1

Genes6

Gene3

Kinase3

Polypeptide2

Cargo proteins1

Similar proteins1

Escherichia3

Macromolecules1

Endoplasmic1

Substrate4

Ligase1

Subunits1

Deficiency2

Cells1

Inhibitor1

Metabolic1

Subunit1

Putative2

Biosynthesis3

Phosphotransferase1

Receptor3

Carboxyl1

Secretory3

BCCP1

Interactions9

Lipid2

Moiety1

UniProtKB1

Esterase2

Signal1

Fatty-acid3

Selectivity1

Anaerobic1

Amino2

Alcohol dehydrogenase1

Distinct1

Glycosylation2

Binds2

Microbial1

Clusters1

Antigenic1

Mass spectrometry1

Substances1

Accumulation2

Innate1

Albumin3

Bacteria1

Monomers1

Binding6

Catalyze1

Molecular3