• Dioxygenases are oxidoreductase enzymes. (wikipedia.org)
  • Two important groups of mononuclear, non-heme iron dioxygenases are catechol dioxygenases and 2-oxoglutarate (2OG)-dependent dioxygenases. (wikipedia.org)
  • The catechol dioxygenases, some of the most well-studied dioxygenase enzymes, use dioxygen to cleave a carbon-carbon bond of an aromatic catechol ring system. (wikipedia.org)
  • Catechol dioxygenases are further classified as being "extradiol" or "intradiol," and this distinction is based on mechanistic differences in the reactions (figures 1 & 2). (wikipedia.org)
  • The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
  • Exploring the catalytic mechanism of the extradiol catechol dioxygenases. (warwick.ac.uk)
  • For example, in the α-ketoglutarate-dependent enzymes, one atom of dioxygen is incorporated into two substrates, with one always being α-ketoglutarate, and this reaction is brought about by a mononuclear iron center. (wikipedia.org)
  • Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
  • In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
  • Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)
  • It exhibited a half life of 30 min at 80 C and 81 min at 75 C, making it the most thermostable extradiol dioxygenase studied. (muni.cz)
  • Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
  • Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites. (openmicrobiologyjournal.com)
  • In silico analyses showed efficient binding of ß- and δ-isomers with enzymes leading to rapid degradation that need further validation by cloning and biochemical experiments. (bvsalud.org)
  • Further, the centralized degradation pathways mean synthesis of fewer degradative enzymes requiring less metabolic energy. (biomedcentral.com)
  • Intradiol enzymes cleave the carbon-carbon bond between the two hydroxyl groups. (wikipedia.org)
  • The most widely observed cofactor involved in dioxygenation reactions is iron, but the catalytic scheme employed by these iron-containing enzymes is highly diverse. (wikipedia.org)
  • Herein, we found that intracellular enzymes were the major contributors to E2 biodegradation by strain ES2-1. (bvsalud.org)
  • Enzymes employ many different schemes to use dioxygen, and this largely depends on the substrate and reaction at hand. (wikipedia.org)
  • The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
  • A 3-D homology model of BphC_JF8 provided a basis for understanding the substrate specificity, quaternary structure and stability of the enzyme. (muni.cz)
  • The entries are also linked to several external database including BRENDA, KEGG, ENZYME and UM-BBD providing wide background information. (biomedcentral.com)
  • Each enzyme entry contains its common name and synonym, reaction in which enzyme is involved, family and subfamily, structure and gene link and literature citation. (biomedcentral.com)
  • Extradiol members utilize ferrous iron as the active redox state, and this center is commonly coordinated octahedrally through a 2-His-1-Glu motif with labile water ligands occupying empty positions. (wikipedia.org)
  • Intradiol enzymes cleave the carbon-carbon bond between the two hydroxyl groups. (wikipedia.org)
  • Advanced oxidation processes (APOs) of aromatic compounds, particularly of phenol, yield several benzene derivatives, such as hydroquinone, catechol, and resorcinol, as intermediate metabolites of its transformation. (hindawi.com)
  • C230 purified from strains W31, PKO1, and CFS215 had a significantly greater affinity for oxygen as well as a significantly greater rate of substrate turnover than found for the analogous enzymes from the TOL plasmid (pWW0) of Pseudomonas putida PaW1, from Pseudomonas cepacia G4, or from P. putida F1. (nih.gov)