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  • reaction
  • The two enzymes can be distinguished based on their reaction products and cofactors. (wikipedia.org)
  • If you click on the box you will change to the appropriate reaction scheme or enzyme specification. (qmul.ac.uk)
  • An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, shown below. (wikipedia.org)
  • The optimum pH for an enzymatic reaction may shift by as much as 3 pH units upon immobilization.24 This shift is a result of both the charge of the support material and the chemical modification of the enzyme. (clicktocurecancer.info)
  • section describes the catalytic activity of an enzyme, i.e. the chemical reaction it catalyzes. (uniprot.org)
  • In enzymology, a lactoylglutathione lyase (EC 4.4.1.5) (also known as glyoxalase I) is an enzyme that catalyzes the isomerization of hemithioacetal adducts, which are formed in a spontaneous reaction between a glutathionyl group and aldehydes such as methylglyoxal. (wikipedia.org)
  • The reaction of 1 and catechol in the presence of excess triethylamine gave the catecholate (CAT) chelate b is(u -al koxo) -bridged dinuclear iron(Ill) complex [Fe(L)(CAT)12 (2). (diva-portal.org)
  • For example, in the α-ketoglutarate-dependent enzymes, one atom of dioxygen is incorporated into two substrates, with one always being α-ketoglutarate, and this reaction is brought about by a mononuclear iron center. (wikipedia.org)
  • The oxidation of the catechol 3',4'-dihydroxyl electron-donating groups occurs first, at very low positive potentials, and is a reversible reaction. (wikipedia.org)
  • aromatic compounds
  • Six species of free-living nitrogen fixing bacteria, Azomonas agilis, Azospirillum brasilense, Azospirillum lipoferum, Azotobacter chroococcum, Azotobacter vinelandii , and Beijerinckia mobilis , were surveyed for their ability to grow and fix N 2 using aromatic compounds as sole carbon and energy source. (springer.com)
  • Dorn E, Knackmuss H-J (1978) Chemical structure and biodegradability of halogenated aromatic compounds: two catechol 1,2-dioxygenases from a 3-chlorobenzoate grown pseudomonad. (springer.com)
  • iron enzymes
  • His group is also working towards designing functional models for iron enzymes and capturing, observing, and categorizing highly active metal-based intermediates. (wikipedia.org)
  • intradiol
  • However, the enzyme preparations from Brevibacterium and Arthrobacter have only the intradiol cleavage activity. (wikipedia.org)
  • HL = NN-bis(2-pyridylmethyl)-N-(2hydroxyethyl)amine] of the tripodal N3O ligand was prepared as a biomimetic model for the intradiol-cleaving dioxygenase enzymes. (diva-portal.org)
  • Complex 1 displays the intradiol-cleaving dioxygenase activity, and the coordinate ethoxyl arm of the ligand is capable of accepting the proton from catechol, which mimics the function of Tyr447 in the. (diva-portal.org)
  • Intradiol enzymes cleave the carbon-carbon bond between the two hydroxyl groups. (wikipedia.org)
  • NADH
  • The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate and NAD+ to produce 3-methylcatechol, NADH and CO2. (wikipedia.org)
  • phenol
  • Catechol is produced industrially by the hydroxylation of phenol using hydrogen peroxide. (wikipedia.org)
  • Catechin /ˈkætɪtʃɪn/ is a flavan-3-ol, a type of natural phenol and antioxidant. (wikipedia.org)
  • hydroxyl
  • 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
  • In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
  • The second catechol hydroxyl group on carbon 3 (C3) is coordinated to Fe3+ after its deprotonation by the Tyr200 ligand. (wikipedia.org)
  • Catechin possesses two benzene rings (called the A- and B-rings) and a dihydropyran heterocycle (the C-ring) with a hydroxyl group on carbon 3. (wikipedia.org)
  • The ability to quench singlet oxygen seems to be in relation with the chemical structure of catechin, with the presence of the catechol moiety on ring B and the presence of a hydroxyl group activating the double bond on ring C. Electrochemical experiments show that (+)-catechin oxidation mechanism proceeds in sequential steps, related with the catechol and resorcinol groups and the oxidation is pH-dependent. (wikipedia.org)
  • Those enzymes mediate the O-methylation on a specific hydroxyl group, like on 4' (example in Catharanthus roseus) or 3' (example in rice) positions. (wikipedia.org)
  • catalytic
  • The enzyme resembles a boomerang in shape, and can therefore be clearly divided into three domains: two catalytic domains residing at each end of the "boomerang" and a linker domain at the center. (wikipedia.org)
  • The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
  • Particle size is also an important consideration: smaller particles have been observed to yield catalytic properties more closely approximating those obtained with soluble enzymes. (clicktocurecancer.info)
  • The most widely observed cofactor involved in dioxygenation reactions is iron, but the catalytic scheme employed by these iron-containing enzymes is highly diverse. (wikipedia.org)
  • XylE
  • In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
  • XylE is inefficient on its own, especially in vitro , due to instability caused by oxygen sensitivity, and oxidation of the active site iron molecule during it's functional cycle rendering the enzyme inactive 8 . (igem.org)
  • Nitrogen
  • strain JS42 is able to utilize 2-nitrotoluene (2NT) as its sole carbon, nitrogen, and energy source. (nih.gov)
  • All heteroatoms (N3O) of the ligand are coordinated to the iron center in complex 1 with two pyridine nitrogen atoms on the axial bonds, while one of the pyridyl arms of the ligand is left uncoordinated in complex 2. (diva-portal.org)
  • carbon-sulf
  • Glyoxalase I is classified as a carbon-sulfur lyase although, strictly speaking, the enzyme does not form or break a carbon-sulfur bond. (wikipedia.org)
  • bacteria
  • 3. The composition of claim 1 wherein the host organism comprises bacteria of the Bacillus or Clostridia genera. (patents.com)
  • oxygenation
  • 1. Hayaishi, O. Direct oxygenation by O 2 , oxygenases. (qmul.ac.uk)
  • Despite this common oxygenation event, the mononuclear iron dioxygenases are diverse in how dioxygen activation is used to promote certain chemical reactions. (wikipedia.org)
  • organic compounds
  • Urushiols are naturally existing organic compounds that have the catechol skeleton structure and diphenol functionality but with alkyl groups substituted onto the aromatic ring. (wikipedia.org)
  • The byproducts are stored in the form of tailings ponds where the water contains (1) toxic organic compounds such as naphthenic acids and catechol, (2) heavy metals, and (3) fine clay sediment 1 . (igem.org)
  • To address the decontamination of (1) toxic organic compounds such as naphthene and catechol found in the tailings ponds, natural pathways that can break down the toxic chemicals can be utilized. (igem.org)
  • pathways
  • in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. (wikipedia.org)
  • flavan-3-
  • The single-molecule (monomer) catechin, or isomer epicatechin (see diagram), adds four hydroxyls to flavan-3-ol, making building blocks for concatenated polymers (proanthocyanidins) and higher order polymers (anthocyanidins). (wikipedia.org)