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  • microRNA
  • We are also interested in (3) protein-RNA complexes along the microRNA (miRNA) biogenesis pathway that mediate processing of primary miRNAs to their precursor counterparts, and processes associated with miRNA guide strand-mediated cleavage, translation inhibition or degradation of target RNAs. (mskcc.org)
  • Transcription
  • During the viral life cycle, viral RNA is present in the cytoplasm of cells after fusion and before reverse transcription, which presents a target that when acted on can inhibit infection before proviral integration. (jimmunol.org)
  • In nature complementarity is the base principle of DNA replication and transcription as it is a property shared between two DNA or RNA sequences, such that when they are aligned antiparallel to each other, the nucleotide bases at each position in the sequences will be complementary, much like looking in the mirror and seeing the reverse of things. (wikipedia.org)
  • Complementarity is also utilized in DNA transcription, which generates an RNA strand from a DNA template. (wikipedia.org)
  • The sense strand is, generally, the transcribed sequence of DNA or the RNA that was generated in transcription. (wikipedia.org)
  • Silencing
  • RNA silencing mediated antiviral immunity is recognized as a broad spectrum pathogen associated molecular pattern (PAMP) triggered immunity (or PTI). (frontiersin.org)
  • In the past decade, extensive studies have been undertaken and have uncovered a comprehensive genetic frame-work of RNA silencing mediated viral immunity. (frontiersin.org)
  • However, successful virus infection can overcome antiviral RNA silencing. (frontiersin.org)
  • Structural biology of RNA silencing and its functional implications. (mskcc.org)
  • A major role for PIKfyve in synthesis of cellular PtdIns5P is suggested by data for reduced PtdIns5P mass levels upon heterologous overexpression of the enzymatically inactive PIKfyve point-mutant (PIKfyveK1831E) and PIKfyve silencing by small interfering RNAs. (wikipedia.org)
  • While some functions of RNA silencing and its machinery are understood, many are not. (wikipedia.org)
  • For example, RNA silencing has been shown to be important in the regulation of development and in the control of transposition events. (wikipedia.org)
  • RNA silencing has been shown to play a role in antiviral protection in plants as well as insects. (wikipedia.org)
  • Also in yeast, RNA silencing has been shown to maintain heterochromatin structure. (wikipedia.org)
  • demonstrating that double-stranded RNA could act as a trigger for gene silencing. (wikipedia.org)
  • Since then, various other classes of RNA silencing have been identified and characterized. (wikipedia.org)
  • It has also been observed that the major RNA silencing pathways currently identified have mechanisms of action which may involve both post-transcriptional gene silencing (PTGS) as well as chromatin-dependent gene silencing (CDGS) pathways. (wikipedia.org)
  • CDGS involves the assembly of small RNA complexes on nascent transcripts and is regarded as encompassing mechanisms of action which implicate transcriptional gene silencing (TGS) and co-transcriptional gene silencing (CTGS) events. (wikipedia.org)
  • strand
  • Sense strand and spacer (i.e. small sequence of loop present between antisense and sense strands). (ukessays.com)
  • The majority of antisense drugs function through the RNase H-dependent mechanism, in which RNase H hydrolyzes the RNA strand of the DNA/RNA heteroduplex. (wikipedia.org)
  • Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. (wikipedia.org)
  • The presence of this functional group causes the helix to mostly adopt the A-form geometry, although in single strand dinucleotide contexts, RNA can rarely also adopt the B-form most commonly observed in DNA. (wikipedia.org)
  • A complementary strand of DNA or RNA may be constructed based on nucleobase complementarity. (wikipedia.org)
  • Self-complementarity refers to the fact that a sequence of DNA or RNA may fold back on itself, creating a double-strand like structure. (wikipedia.org)
  • Ribose
  • While DNA contains deoxyribose, RNA contains ribose (in deoxyribose there is no hydroxyl group attached to the pentose ring in the 2' position). (wikipedia.org)
  • An important structural feature of RNA that distinguishes it from DNA is the presence of a hydroxyl group at the 2' position of the ribose sugar. (wikipedia.org)
  • dsRBD
  • In addition to these domains, hsDicer contains four other functional domains: two RNaseIII domains and two double stranded RNA binding domains (DUF283 and dsRBD). (wikipedia.org)
  • complex
  • Both pathways initially involve poly(A) shortening which is mediated predominantly by an enzyme complex containing Ccr4p and Caf1p ( 40 ). (asm.org)