• Her main research interests have been the study of Mur ligases from different bacterial species and the search for inhibitors of these enzymes. (degruyter.com)
  • His main research interests are the structure-based design, synthesis and evaluation of small-molecule enzyme inhibitors and receptor ligands. (degruyter.com)
  • For more than 30 years, his main research interests have been the functional and structural study of the enzymes of peptidoglycan biosynthesis, the determination of the structure of peptidoglycan from certain bacterial species, and the search for peptidoglycan biosynthesis inhibitors that could act as antibacterial agents. (degruyter.com)
  • In this review, we describe the results of research studies reported since 2018, giving particular emphasis to the use of fluorescent probes for detection and imaging of glycosidases, activity-based probes for covalent labelling of these enzymes, glycosidase inhibitors, and glycosidase-activatable prodrugs. (bvsalud.org)
  • The synthesis of the peptide stem of bacterial peptidoglycan involves four enzymes, the Mur ligases (MurC, D, E and F). Among them, MurD is responsible for the ATP-dependent addition of d -glutamic acid to UDP-MurNAc- l -Ala, a reaction which involves acyl-phosphate and tetrahedral intermediates. (degruyter.com)
  • Neueste Experimente zeigten, dass Feglymycin auch die zytoplasmatischen Enzyme MurA und MurC der bakteriellen Zellwandbiosynthese inhibiert. (tu-berlin.de)
  • Ein Alanin-Scan als eine erste Studie zur Struktur-Aktivitäts-Wirkungsbeziehung ergab, dass lediglich die Deletion der aciden Aminosäureseitenkette der C-terminalen Asparaginsäure zu einem leichten Abfall der antiretroviralen Aktivität sowie der Inhibitionswirkung auf die Enzyme MurA und MurC führt. (tu-berlin.de)
  • Latest experiments also revealed that Feglymycin inhibits the cytoplasmatic enzymes MurA and MurC of the bacterial cell wall biosynthesis. (tu-berlin.de)
  • His main research interests concern the identification of genes and enzymes involved in this pathway, the regulation of this metabolism, the mode of action of antibiotics and bacteriocins interfering with this pathway and the corresponding resistance mechanisms, as well as the molecular bases for the recognition of bacterial cell-wall peptidoglycan by host innate immunity mechanisms. (degruyter.com)
  • Like most enzymes of peptidoglycan biosynthesis, MurD constitutes an attractive target for the design and synthesis of new antibacterial agents. (degruyter.com)
  • One important driver of matrix tension is lysyl oxidase-like 2 (LOXL2), an enzyme that catalyses covalent cross-linking of ECM molecules, including fibrillar collagens. (ersjournals.com)
  • MCs can secrete nociceptor sensitizing and activating agents, such as serotonin, prostaglandins, histamine, and proteolytic enzymes that can also activate the pain-mediating transient receptor potential vanilloid channels. (frontiersin.org)
  • c) Design and construction of functionalized self assembled DNA and RNA nanostructures capable of complex biosensing, coupled with controlled action e.g. drug release, enzyme activation, and receptor signaling. (au.dk)
  • An enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance. (colorado.edu)
  • Rutin prevents experimental selenite-induced cataractogenesis in rat pups, possibly by preventing depletion of antioxidant enzymes and of GSH, and by inhibiting lipid peroxidation. (molvis.org)
  • protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
  • Cnicin blocks the enzymes necessary for bacteria replication so they are not able to reproduce and die. (wikipedia.org)
  • In general, FosA and FosX enzymes are produced by Gram-negative bacteria, whereas FosB is produced by Gram-positive bacteria. (mdwiki.org)
  • In vitro imaging studies confirmed their enzyme-activatable therapy response tracking abilities both in cancer cells and tumor spheroids. (ntno.org)
  • The Company focuses on oral treatments for rare diseases, in which unmet medical needs exist and an enzyme plays the key role in the biological pathway of the disease. (webull.com)
  • by University of Manchester Researchers from the Manchester Institute of Biotechnology (MIB) have developed a new enzyme engineering platform toimprove plastic degrading enzymes through directed evolution. (plasticstar.io)
  • These enzymes function by nucleophilic attack on carbon 1 of fosfomycin, which opens the epoxide ring and renders the drug ineffective. (mdwiki.org)
  • After 5-days, two hepatic-veins resulted recanalized in association with amelioration of liver-enzyme/function and aPL quantification. (isth.org)
  • Now the clever design of Mura Pathway's Dandelion Chai is that it is blended with other herbs in such a complimentary way so that you don't know you are drinking a bitter digestive tonic. (murapathway.com.au)
  • An enzyme variant created by engineers and scientists at The University of Texas at Austin can break down environment-throttling plastics that typically take centuries. (plasticstar.io)
  • Intrinsic resistance to antimicrobials has traditionally been attributed to reduced permeability of the cell envelope, presence of inactivating enzymes or efflux pumps that can extrude the antimicrobial agents ( Cox and Wright, 2013 ). (frontiersin.org)
  • These enzymes play a vital role in a wide variety of biological events, such as digestion of nutritional carbohydrates, lysosomal catabolism of glycoconjugates, and posttranslational modifications of glycoproteins. (bvsalud.org)
  • Owing to the physiological and pathological significance of glycosidases, the development of small molecules that target these enzymes is an active area in glycoscience and medicinal chemistry. (bvsalud.org)
  • When they are stimulated they cause an increase in gastric acid and enzymes which help us to digest our food effectively (Bone & Mills, 2013). (murapathway.com.au)
  • Prior work has established that Cys115 of Escherichia coli and Enterobacter cloacae MurA is the active site nucleophile alkylated by fosfomycin and implicated this residue in the formation of a covalent phospholactyl-enzyme adduct derived from substrate, phosphoenolpyruvate (PEP). (nih.gov)
  • On the basis of sequencing information from putative MurA homolog from Mycobacterium tuberculosis, we generated a C115D mutant of E. coli MurA that was highly active but fully resistant to time-dependent inhibition by fosfomycin. (nih.gov)
  • In this study, we were interested in the inhibition of this enzyme by a computational approach, virtual screening, followed by an in vitro test of the best molecules with high affinities for the enzyme on four pathogenic bacteria: Escherichia coli ATCC25922, Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC25923, and Methicillin resistant Staphylococcus aureus ATCC33591. (edu.dz)
  • SEDS protein RodA was recently discovered in Bacillus subtitle as a new GT enzyme, and it has shown to play a similar role in E coli. (javatpoint.com)
  • Our results revealed that transcription factor Nrf2 could induce CBR1 transcription in liver of mice during I/R. Pre-treatment with sulforaphane, an activator of Nrf2, increased CBR1 expression, decreased liver enzymes such as aspartate aminotransferase and alanine transaminase, and reduced I/R-related pathological changes. (molcells.org)
  • Fosfomycin still bound to the active site of C115D MurA, as established by the observed reversible competitive inhibition by fosfomycin. (nih.gov)
  • Fosfomycin, a well known only drug that target this enzyme. (osdd.net)
  • 14. The impact of different antioxidant agents alone or in combination on reactive oxygen species, antioxidant enzymes and cytokines in a series of advanced cancer patients at different sites: correlation with disease progression. (nih.gov)
  • Our recently published studies have demonstrated that: ROS levels and antioxidant enzymes activity (mainly Glutathione Peroxidase, GPx), serum levels of proinflammatory cytokines, leptin and C Reactive Protein of cancer patients are correlated with the most important clinical indexes of disease such as Stage and ECOG PS. (unica.it)
  • Squadrone S, Brizio P, Mancini C, Pozzi E, Cavalieri S, Abete MC, Brusco A. (2015) Blood metal levels and related antioxidant enzyme activities in patients with ataxia telangiectasia. (unito.it)
  • In agreement with these results, cytotoxic assays have revealed that PALQ has limited activity, whereas the single mutants P19A and L28Q display cytotoxic activity against malignant cells almost as large as the wild-type enzyme. (cnr.it)
  • The significant increase in the antitumor activity, brought about by the substitution of just two residues in going from the double mutant to the wild-type enzyme, suggests a new strategy to improve this important biological property by strengthening the interface that stabilizes the quaternary structure of NCD-BS. (cnr.it)
  • Mutation of Q492L, V493L, S494A resulted in only 30, 50, and 70% inhibition of the activity by PPPA, respectively (as opposed to greater than 95% with the wild type enzyme), suggesting these three residues are responsible for the selective inhibition by PPPA of ACAT2. (elsevierpure.com)
  • We have prepared and fully characterized by X-ray diffraction the double mutant P19A/L28Q (PALQ) of the seminal enzyme. (cnr.it)
  • This finding, while disclosing a potential specific role for blLAP in glutathione metabolism, raised a question about the features required for molecules to be a substrate for the enzyme. (cnr.it)
  • Statistical differences between kinetic parameters for vancomycin-resistant enterococci WT and C119D MurA were assessed by using a paired t -test. (cdc.gov)
  • in press), which sug- gests that the colicins exert their inhibitory role on specific cellular functions while remaining on the surface of the bacterial cell, and that at least the primary inhibitory functions are reversible by removal of colicin with proteolytic enzymes. (nih.gov)
  • Surprisingly, the cytotoxic and antitumor activity of the enzyme is a peculiar property of the swapped dimer. (cnr.it)
  • These enzymes are involved in enzymatic pathways that are absent from animals and thus, are attractive targets for the development of novel antimicrobials. (emporia.edu)
  • To clarify the interaction of the enzyme with sulfhydryl-containing derivatives, (Zn/Zn) - and (Mn/Zn) blLAP forms were prepared and functional-structural studies were undertaken. (cnr.it)