Enzyme mechanism enzyme stability. Medical search. Frequent questions

Explain The Various Ways That Enzymes Can Be Regulated, Including Post-Translational Modifications Of Proteins And Their Degradation. (uaeu.ac.ae)
Her scientific pursuit focuses on the biochemical mechanisms of chromosome replication proteins, and molecular mechanisms of their involvement in maintaining genome integrity during chromosome replication. (wikipedia.org)
Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
SUMOylation of proteins is an important mechanism in cellular responses to environmental stress ( 21 , 22 ). (frontiersin.org)
Catalytically inactive proteins have been converted into modestly catalytic ones for two different reactions, but the observed enhancements of rate 6 , 7 were only about a millionth of those produced by naturally occurring enzymes. (nature.com)
Now, scientists at Princeton have used 'designer chromatin' templates - highly customized replicas of cellular DNA and histone proteins, the scaffolding proteins around which DNA is wrapped - to reveal new details about Suv39h1's mechanism. (princeton.edu)
In such a scenario, a set of enzymes (proteins that act as catalysts for biochemical reactions) are activated to repair the damage. (phys.org)
Enzymes liable for the hydrolysis of proteins down to amino acids are the proteases. (rockinhibitor.com)
A second major area of interest focuses on how proteins contribute to RNA stability and degradation. (mskcc.org)
In the current study, we used a crude preparation of reticulocyte proteins, which contains ubiquitin-conjugating enzymes and the proteasome, to determine how nNOS is labilized. (aspetjournals.org)
Juice and beverage clarification Likewise, the enzyme can also be applied to solve cold haze problem and extend shelf life stability of juice or beverage products by damaging haze sensitivity of proline-containing proteins. (enzymes.bio)
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. (enzymes.bio)

Describe the kinetics, mechanism and role of enzymes in living systems. (uaeu.ac.ae)
7. Analysis of multiparameter data in enzyme kinetics using N-dimensional coordinate systems. (scialert.net)
The research in the Enzyme Engineering group focuses on the improvement of the biocatalytic capacity of enzymes through protein engineering approaches, enzyme discovery, computational tools, enzyme kinetics and mechanistic enzymology. (au.dk)

Furthermore, the role of DNA thermodynamic "signatures" in lesion recognition by DNA repair enzymes, such as DNA glycosylases, is poorly understood, again because such enzymes catalyze the repair of these lesions, precluding their biochemical/structural study in corresponding protein/DNA complexes. (hhs.gov)
These properties will then be compared to biochemical data, such as equilibrium binding constants to DNA glycosylases AAG and AlkD, and will be utilized as structural probes to gain insight regarding recognition and excision mechanisms employed by these enzymes. (hhs.gov)
It is also sometimes difficult to prove that designer enzymes are truly catalytic on the basis of biochemical observations, and some exciting claims have been found to be flawed. (nature.com)
These biochemical modifications impair the activities, and possibly also the stability, of caspase 8 and 3 and thereby weaken the capacity of these enzymes to induce apoptosis. (lu.se)

One popular view is that protein cofactors acted as catalysts prior to the evolution of the protein enzymes with which they are now associated. (darkenergybiosphere.org)
Although enzymes are superb catalysts, their range of reactions is limited to those that support life. (nature.com)
Enzymes are astoundingly good catalysts: they allow reactions to occur billions of times faster than would be possible without them, at temperatures much lower than those required by typical synthetic catalysts. (nature.com)
Enzymes have great advantages over chemical catalysts, such as capability to produce a versatile set of high-value products, excellent regio- and stereo-selectivity, reusability, ambient conditions for activity leading to energy saving and biodegradability. (au.dk)
Deciphering enzyme mechanisms also inspire new chemistries and new catalysts. (au.dk)

Biocatalytic kinetic effect of α -chymotrypsin enzyme has been investigated in its free and pretreated forms (it was covered by a very thin, porous polymer layer, called enzyme nanoparticle) as well as its immobilized form into pores of polysulfone/polyamide asymmetric, hydrophilic membrane. (mdpi.com)
The reconstituted ancestral enzymes have been shown to be valuable tools in our understanding of the evolution of biocatalytic mechanisms. (europa.eu)

Rational bioengineering attempts to create new RLuc variants with fine-tuned bioluminescent properties are however hampered by the fact that its catalytic reaction mechanism remains poorly understood. (europa.eu)
However, the regulatory mechanisms of HPV infection and consequent immune escape reaction, persistent infection and cell proliferation and the regulatory mechanisms of vascular proliferation are poorly understood and require additional investigation ( 4 ). (spandidos-publications.com)
Neutrophil apoptosis can be modulated by several factors in the local environment, such as the Fas ligand (FasL), but the molecular mechanisms involved are poorly understood. (lu.se)

Moreover, the enzymes created by ASR often exhibit enhanced thermal stability and promiscuous enzymatic properties, which can be useful in various industrial settings. (europa.eu)
Now, scientists led by Dr. Gwangrog Lee from the Gwangju Institute of Science and Technology (GIST) in Korea have utilized the latest technology in single molecule detection to study enzymatic interactions and observe the mechanism of BER, filling the gap in our understanding of this coordination mechanism. (phys.org)
We aim to explore not only natural enzymatic transformations, but also potential non-natural reactions through substrate engineering and enzyme reprogramming. (au.dk)
They also develop specific strategies to increase ROS resistance, which include deviation of the glycolytic flux into the pentose phosphate pathway (PPP) or changes in other enzymatic mechanisms enhancing ROS detoxification [ 3 , 4 ]. (hindawi.com)

Dr. Lee highlights the crux of the study, saying, "Interestingly, there is perfect temporal and spatial regulation between the gap creation activity of ExoIII and the gap filling activity of Pol I, such that genomic stability is always maintained. (phys.org)
Membrane structure and function: Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport, ion pumps, mechanism of sorting and regulation of intracellular transport, electrical properties of membranes. (pathfinderacademy.in)
My seminal work into enzyme mechanisms, regulation and engineering received international acclaim evidenced by invitations to provide plenary and keynote lectures and review articles in prestigious journals. (edu.au)
In this dissertation thesis, I describe and elucidate intracellular signalling mechanisms that are involved in regulation of spontaneous and Fas-induced apoptosis in human neutrophils. (lu.se)

Recent advances in genetic engineering, structural biology and bioinformatics render us the chance to engineer existing enzymes as well as to discover new enzymes to obtain biocatalysts that are more efficient for industrial and medical purposes. (au.dk)

We investigated the stability of acetyl coenzyme A (Acetyl Co-A, the group transfer cofactor in citric acid synthesis in the TCA cycle) under early Earth conditions, as well as whether Acetyl Co-A or its small molecule analogs thioacetate or acetate can catalyze the transfer of an acetyl group onto oxaloacetate in the absence of the citrate synthase enzyme. (darkenergybiosphere.org)
Trimethoxysilyl and acrylamide-bisacrylamide polymers have been used for synthesis of enzyme nanoparticles. (mdpi.com)
This suggests that digestive enzyme synthesis and secretion are controlled through the digestive procedure (Lehane et al. (rockinhibitor.com)
I have successfully focused my research on the investigation of enzyme-catalysed reactions, their optimisation for applications in biotechnology and the synthesis of small molecules that mimic their structure and catalytic function ( i.e. biomimetics). (edu.au)

The subsequent mutagenesis of this plastic residue leads to generation of a mutant enzyme SanSyn F441V which can produce both α - and β -santalenes. (nature.com)

One of the major intentions of this project was to determine atomic-level structures of the dual-function ancestral enzyme ancHLD-RLuc complexed with bound substrate molecules, with emphasis on coelenterazine-powered monooxygenation (luciferase) catalysis. (europa.eu)

Graphene Flagship researchers show how graphene oxide suspended in water biodegrades in a reaction catalysed by a human enzyme, with the effectiveness of the breakdown dependent on the colloidal stability of the suspension. (phys.org)
Beer colloidal stability improvement The enzyme can be used to substitute PVPP in the prevention of cold haze in beer without harming foaming property of beer and health concerns. (enzymes.bio)

Researchers have discovered the two-step process that activates an essential human enzyme, called Suv39h1, which is responsible for organizing large portions of the DNA found in every living cell. (princeton.edu)
Graphene oxide suspensions before and after degradation by a human enzyme. (phys.org)

Their participation in the molecular mechanisms of resistance to cisplatin, a drug commonly used in chemotherapy, is also revised. (hindawi.com)

For advances in protein structure and enzyme mechanisms by use of affinity labeling and site-directed mutagenesis. (ornl.gov)
Ionizable residues in amino acids have been shown to play vital roles in protein binding to other molecules and in enzyme mechanisms, exerting considerable influence on protein structure, stability, and solubility [14,15,16]. (american-jiras.com)

As the primary microbial substrate after shoot cutting, the element stoichiometry of root-detritus (dying or dead roots) influences the enzyme activity in root-detritusphere. (springer.com)
Cai XQ, Lin ZW, Penttinen P, Li YF, Li YC, Luo Y, Yue T, Jiang PK, Fu WJ (2018) Effects of conversion from a natural evergreen broadleaf forest to a Moso bamboo plantation on the soil nutrient pools, microbial biomass and enzyme activities in a subtropical area. (springer.com)
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish. (enzymes.bio)

After detailing the findings of their experiment, the researchers discuss the mechanism of graphene oxide degradation, beginning with a summary of the process by which myeloperoxidase acts against infection by microbes and other invasive materials that inflame biological tissues. (phys.org)
Surface electric charge is also thought to contribute, as it does in the case of oxidised carbon nanotubes, since it favours the strong binding of graphene oxide with the enzyme, and subsequently triggers its degradation. (phys.org)
The mechanism of this selective proteolytic degradation, or in essence how the nNOS becomes labilized and recognized for ubiquitination and subsequent degradation, has not been determined. (aspetjournals.org)
Treatment of nNOS with N G -nitro- l -arginine or 7-nitroindazole led to stabilization of the dimeric nNOS and decreased proteasomal degradation of the enzyme, consistent with that observed in cells. (aspetjournals.org)

Categorize Biological Membranes And Various Mechanisms Of Transport Of Molecules Through Membranes. (uaeu.ac.ae)
Strength of evidence for public health has a classical hierarchy that has dose-response relationship at the top and biological mechanism at the bottom, Hill (1965). (whale.to)
For this bioluminescent effect, the RLuc luciferase is commonly used as a reporter enzyme in cell biological research and bioimaging technologies. (europa.eu)
But enzymes have evolved to accelerate only biological reactions, under the narrow set of conditions that are compatible with life. (nature.com)
1 have computationally designed and prepared the first enzyme capable of catalysing a non-biological reaction. (nature.com)
This biological process - and the enzymes that control it - had been studied for 30 years prior, but remained a mystery to researchers in the field until their work was described in two related studies published in Nature and the Journal of the American Chemical Society . (mskcc.org)
The unique property makes it very beneficial in brewing and beverage to prevent cold haze and improve non-biological stability. (enzymes.bio)

They describe a method for designing enzymes that catalyse unnatural reactions, and demonstrate its use for two different chemical transformations. (nature.com)
Enzymes work by lowering the activation energy of reactions, specifically by confining substrates in binding sites that stabilize the highest-energy arrangement of atoms in the reaction pathway (known as the transition state). (nature.com)
Furthermore, transition states are modelled using quantum-mechanical calculations, but currently available methods can handle only a limited number of atoms, and are often inadequate for modelling enzyme reactions. (nature.com)
I advanced understanding of the contribution of metals to enzyme-catalysed reactions and employed state-of-the-art technologies ( e.g. cryo-EM, ancestral gene reconstruction) to develop enzyme inhibitors as leads for novel biocides (herbicides, fungicides, antibacterial compounds) and engineer enzymes with enhanced catalytic properties for industrial applications in the advanced manufacturing sector ( i.e. cell-free enzyme cascades, next-gen fertilisers). (edu.au)
Thus, more basic research is needed on the chemical reactions involved in the antigen formation and the immunological mechanisms. (cdc.gov)

Yet despite the rapid advances in the clinical application of diagnostic enzymology, little is known about the basic mechanisms responsible for the enzyme perturbations measured. (marquette.edu)

We succeeded to reconstitute stable complexes of recombinantly-produced and affinity-purified ancHLD-RLuc enzyme complexed with substrate-like and product molecules. (europa.eu)
Unlike the native coelenterazine, the derivative cannot be oxidized, and therefore it represents a perfect chemical tool to study enzyme-substrate complexes. (europa.eu)

Sunson Brewing Enzyme is developed for beer brewing. (sunsonenzymes.com)

and the cells' response to chromosome replication stress and DNA damage to maintain genomic stability. (wikipedia.org)
Dr. Wang's lab also investigated how cells respond to chromosome replication stress and DNA damage to maintain the cells' genomic stability. (wikipedia.org)

Through the measurement of vast numbers of enzymes and isoenzymes, in blood and various other body fluids, a relatively non-invasive method exists for making organ specific diagnosis of various pathologic processes including infarction, inflammation, and tumor invasion as examples. (marquette.edu)
Study of the problem requires investigation of fundamental cell physiology and pathologic alterations of the physiologic processes, while the data generated provides answers to existing clinical situations and opens new avenue for clinical application of enzyme interpretation. (marquette.edu)
The sequence of processes undertaken by the enzymes to fix DNA damage is known as "base excision repair" (BER). (phys.org)
Our comprehensive analysis of the whole genome of F. equiseti provides basic data for studies of gene expression, regulatory and functional mechanisms, evolutionary processes, as well as disease prevention and control. (researchsquare.com)

The maintenance of the steady-state equilibrium between ROS generation and elimination is crucial for cell survival, while its loss causes cell death by different mechanisms triggered by oxidative damage. (hindawi.com)
The researchers found a way of initiating effective oxidative degeneration of cellulose using the copper-dependent TaGH61 enzyme to overcome the chemical inertness of the material. (wun.ac.uk)

Her lab was the first to use reverse-genetic approach to isolate the cDNA clones encoding the catalytic polypeptide of the principal chromosome replication initiation enzyme, DNA polymerase alpha, of human cells. (wikipedia.org)
BER is mainly carried out by the exonuclease III (ExoIII) and polymerase I (Pol I) enzymes. (phys.org)

Citations: Additional Possibility of Data Analysis of Enzyme Inhibition and Activation 3. (scialert.net)

We are particularly interested in discovering new biocatalysts with industrial potential and modifying known enzymes for enhanced production technologies, including cascade reaction systems, of high-value chemicals and of medically important molecules. (au.dk)
Adaptive immune mechanisms recognize and neutralize foreign molecules or microorganisms in a specific manner. (lu.se)

Antioxidant enzyme Glutathione S- Transferase (GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. (prospecbio.com)

However, even though free cofactors may be stable in a geological environmental setting, an enzyme or other mechanism to promote reaction specificity would likely be necessary for at least this particular reaction to proceed. (darkenergybiosphere.org)
Due to its high specificity, the enzyme will bring no harm to other parts of protein, keeping all other functionalities of protein. (enzymes.bio)

The expression of genes through miRNA is regulated by endogenous regulatory pathways, resulting in high stability and biocompatibility ( 5 ). (spandidos-publications.com)

In 2010, Dr. Lima - in collaboration with chemist Derek S. Tan and a multidisciplinary team of researchers at Memorial Sloan Kettering - determined the mechanism for how a family of enzymes called E1 activates ubiquitin, shedding light on a complex reaction that plays a key role in regulating cellular behavior. (mskcc.org)

E6-AP) is an important member of the ubiquitin proteasome system and a type of ubiquitin protein ligase (E3 enzyme) ( 6 ). (spandidos-publications.com)

Based on our unique structural data we were able to propose the catalytic mechanism of conversion of coelenterazine into coelenteramide, accompanied with emission of blue light, at alpha/beta-hydrolase fold. (europa.eu)

Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
We hypothesised that P fertilisation would decrease the C/P ratio of root-detritus, therefore affecting the hotspot areas and hot moments of C-acquiring and P-acquiring enzyme activities, as well as their activity ratio (C/P acquisition ratio). (springer.com)
This is mostly due to lack of improvement of efficient enzymes that suit industrial needs such as activity levels, stability, reusability and cost effectiveness. (au.dk)
Professor Paul Dupree of the University of Cambridge Bioenergy Initiative and Director of the BBSRC Sustainable Bioenergy Cell Wall Sugars programme, said "Understanding the GH61 enzyme activity is one of the most significant recent advances in the area of biomass deconstruction and release of cell wall sugars. (wun.ac.uk)
Consequently, PP2A can increase the activity of caspase 3 by dual mechanisms and thereby promote the apoptotic response in human neutrophils. (lu.se)
During exposure to organophosphates, AChE and BChE can undergo phosphorylation at their respective active site serine, form OP-ChE adducts and subsequently lose enzyme activity. (cdc.gov)
Serum elevations of ALT activity are rarely observed, except in parenchymal liver disease, since ALT is a more liver-specific enzyme than aspartate aminotransferase (AST). (cdc.gov)

We showed that a non-oxidizable coelenterazine analogue binds to the ancHLD-RLuc enzyme with low nanomolar affinity. (europa.eu)
The XPD protein acts as a helicase, which is an enzyme that attaches (binds) to particular regions of DNA and temporarily unwinds the two spiral strands. (medlineplus.gov)

While other deranged cellular events may also participate in the process, it was anticipated that the results obtained would provide an initial unifying concept concerning the mechanism responsible for enzyme release. (marquette.edu)

According to their different acting mechanisms, DNA topoisomerases can be classified as type I and type II enzymes [ 3 , 6 ]. (jcancer.org)

In Ethanol & Alcohol production process, the starches from agricultural products are converted in fermentable sugars with the help of enzymes. (sunsonenzymes.com)
Working with scientists in Novozymes laboratories at Davis, California, and Bagsvaerd, Denmark, as well as researchers at the University of Copenhagen and the University of Cambridge, they identified the molecular mechanism behind an enzyme found in fungi which can degrade the cellulose chains of plant cell walls to release shorter sugars for biofuels. (wun.ac.uk)

To overcome these limitations, ancestral sequence reconstruction (ASR) represents a powerful approach, in which a hypothetical ancestral sequence of a given present-day enzymes is predicted and reconstructed in a laboratory. (europa.eu)
This project aimed to employ in-lab reconstructed dual-function (dehalogenase/luciferase) ancestral enzyme (ancHLD-RLuc) to decipher molecular evolution steps leading to the functional divergence of modern-day HLD and RLuc enzymes, and to explore how this knowledge could be exploited biotechnologically. (europa.eu)

By constructing stability diagrams showing domains of periodic behavior as a function of the maximum rates of the kinases and phosphatases involved in the two cycles of the cascade, we investigate the role of these converter enzymes in the oscillatory mechanism. (ebi.ac.uk)
For significant advancement of welding science and technology through original and definitive research, particularly for contributions to understanding the solidification behavior of the weld pool, phase stability microstructure-property correlations in welds, and continued leadership and outstanding service to the national and international welding research community. (ornl.gov)

The thermodynamic stability of every sequence and conformation must therefore be calculated to find the lowest-energy structure (that is, the one most likely to form). (nature.com)

Proteases (peptide hydrolases, EC 3.four) are enzymes acting on peptide bonds and include things like the proteinases (endopeptidases, EC 3.four.21-24) along with the exopeptidases (EC 3.two.4.11-19). (rockinhibitor.com)

The purpose of this study was to review the pathophysiology mechanisms and clinical features of arterial and venous thrombosis and to provide guidance for further research on the pathophysiological mechanism, clinical diagnosis, and treatment of venous thrombosis. (frontiersin.org)
The aim is to provide a reference for a comprehensive understanding of venous thrombosis and a scientific understanding of various pathophysiological mechanisms and clinical features related to venous thrombosis, which will contribute to understanding the pathogenesis of intravenous stroke and provide insight into diagnosis, treatment, and prevention. (frontiersin.org)

These characteristics give insight regarding how strongly solvated DNA duplexes containing cationic alkylpurines with minor groove or major groove lesions are, electrostatic/ionic atmosphere changes associated with these modifications, base stacking/pairing interactions, and general B-form conformational stability. (hhs.gov)

TOP2A encodes an enzyme which is implicated in almost any process of DNA metabolism including transcription, replication, movement and untangling [ 3 , 8 , 9 ], which catalyze the passage of two DNA duplexes across each other to resolve the entanglements and coiling of cellular DNA [ 10 ]. (jcancer.org)

Although enzyme mechanisms may differ between humans and mice, gaining a better understanding of DNA damage repair might someday allow us to specifically alter ATM in cancer cells. (nih.gov)
This has led to an improved understanding of the multilayered mechanisms of this pathway transduction proceeds as well as the molecular mechanism regulating this pathway ( 28 ). (frontiersin.org)
For starters, building an accurate model of a transition state requires a detailed understanding of the reaction's mechanism, which isn't always available. (nature.com)
The gene for Kurly has also been detected in relation to human cilia disorders, so the work may have an impact on understanding the mechanisms of human disease, Grimes added. (princeton.edu)
Fully understanding the mechanism behind GH61 is important in the context of commercial production of biofuel from plant waste and a true scientific paradigm shift. (wun.ac.uk)
Understanding these mechanisms is important for manipulating ChAT's function and its involvement in various diseases such as Alzheimer's disease, schizophrenia, and congenital myasthenic syndromes (CMF). (lu.se)

Despite mitigating the high effective charge of the iron(IV) center, the substitution of the MeCN ligand with monoanionic ligands X - decreases the thermal stability of [Fe IV (O)(TPA)] 2+ complexes. (ewha.ac.kr)
In certain metabolic disorders ( ADA and PNP deficiency) enzyme substitution therapy can be applied. (lu.se)

By rearranging the various domains, the research team observed where the enzyme spread its mark across the genome. (princeton.edu)

Jungmin Yoo et al, The mechanism of gap creation by a multifunctional nuclease during base excision repair, Science Advances (2021). (phys.org)

They are also involved in various defense mechanisms within the body. (cdc.gov)

This dissertation investigated the possibility that loss of lysosomal integrity may be the inciting event for release of enzymes from cells. (marquette.edu)

However, very little information regarding the effects of minor versus major groove lesions exist, in part due to their inherent instability with respect to spontaneous and enzyme-mediated depurination. (hhs.gov)

Interestingly, HLD enzymes display remarkable sequence and structural similarity with luciferase from the marine invertebrate Renilla reniformis (RLuc), which reflects their common evolutionary history. (europa.eu)

In this work, we tested the procedures to crosslink functional groups in polar domains of humic acids by using carbodiimide coupling and analyzed to which extent influenced the modification their hydration properties and stability. (researchgate.net)

These anion-substituted complexes model the cis-X-Fe IV =O units proposed in the mechanisms of oxygen-activating nonheme iron enzymes. (ewha.ac.kr)

Specifically, our structures highlight: (i) amino acid residues involved in chemical steps, (ii) residues that deprotonate/reprotonate the coelenteramide to yield blue light (480 nm), and (iii) coordinated motions of enzyme loops carrying aromatic residues that are important for the bulky ligand binding-unbinding turnover. (europa.eu)

This study provides insights for investigation of operational mechanisms of other enzymes involved in DNA repair. (phys.org)

Using enzyme preparation in the beer production process can improve the proportion of the auxiliary materials, reduce the production cost and improve the flavor of beer. (sunsonenzymes.com)

2 are remarkable in the spectacular agreement between their computationally predicted enzyme models and the experimentally determined structures ( Fig. 1 ). (nature.com)
Stability of protein and nucleic acid structures. (pathfinderacademy.in)

A new study in mice shows that this enzyme continues to be activated and function normally, even without a chemical modification previously thought to be necessary. (nih.gov)
The present study aimed to identify the probable anti‑Condyloma acuminata (CA) mechanism of microRNA‑375 (miRNA‑375) in human papillomavirus (HPV). (spandidos-publications.com)
The present study attempted to identify and characterize the probable anti-CA mechanism of miRNA-375 on HPV. (spandidos-publications.com)

To understand how the enzyme carries out this process, the researchers synthesized complex chromatin templates that were three times larger than previously reported models. (princeton.edu)
To that end, flagship researchers led by Alberto Bianco, an organic chemist at the French National Research Council in Strasbourg, have looked in detail at the biodegradation of graphene oxide by an enzyme. (phys.org)
What the researchers found is that highly aggregated suspensions of graphene oxide fail to biodegrade in the presence of myeloperoxidase, but the more stable colloids are completely broken down by the enzyme. (phys.org)

The Hydrogen bonding is critical to DNA stability and reactivity of oligonucleotides. (rutgers.edu)

Poor crystallibility and long-term (>3 months) crystallization process of RLuc appeared to be major drawbacks in the acquisition of structural data that should provide the molecular dissection of RLuc catalytic reaction mechanism. (europa.eu)
The key step in the reaction mechanism of multicopper oxidases (MCOs)--the cleavage of the O-O bond in O2--has been investigated using combined quantum mechanical and molecular mechanical (QM/MM) methods. (lu.se)
The QM/MM calculations were further supported by multireference calculations of the MCO spectroscopic properties20 and combined extended X-ray absorption fine- structure EXAFS/QM/MM calculations.21 This has led to the consensus reaction mechanism depicted in Fig. 1. (lu.se)

ATM's activation was believed to involve a process called autophosphorylation, in which the enzyme would add a phosphate group to itself and then be released from an inactive state. (nih.gov)
Muir, T. W. " A two-state activation mechanism controls the histone methyltransferase Suv39h1 . (princeton.edu)

Organisms1

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes22

Information Science2

Health Care2

Proteins12

Kinetics3

Biochemical4

Catalysts5

Biocatalytic2

Poorly understood3

Enzymatic4

Regulation4

Engineer existin1

Synthesis4

Mutant Enzyme1

Catalysis1

Colloidal stability2

Human enzyme2

Molecular Mechanisms of Resistance1

Protein structure2

Microbial3

Degradation4

Biological7

Reactions5

Enzymology1

Substrate2

Brewing1

Genomic stability2

Processes4

Oxidative2

Polymerase2

Inhibition1

Molecules2

Antioxidant1

Specificity2

Endogenous1

Activates1

Ubiquitin1

Catalytic mechanism1

Activity7

Binds2

Deranged cellular1

Different acting mec1

Sugars2

Ancestral2

Behavior2

Thermodynamic1

Peptide1

Clinical2

Conformational1

Replication1

Understanding6

Substitution2

Genome1

Advances1

Defense1

Dissertation1

Spontaneous1

Evolutionary1

Acids1

Complexes1

Ligand1

Investigation1

Beer1

Structures2

Study3

Researchers3

Reactivity1

Reaction mechanism3

Activation2