• Analysis of reproducibility of the present assay demonstrated 3.96% and 5.85% intra-and inter-assay coefficient of variations, respectively, which were less than those obtained by the commercial kit. (bvsalud.org)
  • The intra-assay CV was below 10% and inter-assay CV below 15% for samples of high, medium and low cortisol concentrations. (biomedcentral.com)
  • Determination of enzyme parameters through calibration is a necessary step to track changing enzyme kinetic characteristics and improves on previous methods to determine label enzyme amounts on the calorimeter platform. (mdpi.com)
  • While this does not allow for the determination of a protein's molecular weight, the microtiter format does allow for a fast, high throughput assay and analysis of hundreds of samples at one time. (advansta.com)
  • Although prostate-specific acid phosphatase is not a cancer-specific enzyme, we conclude that its measurement may be of considerable value in monitoring prostatic disease. (aaccjnls.org)
  • This assay is based on measurement of activity of lactate dehydrogenase (LDH). (edu.au)
  • The assay was optimized with defined negative and positive bovine sera, where positive sera inhibited the binding of the epitope-specific monoclonal antibody BABB75A4. (asm.org)
  • Protein array is a versatile high-throughput assay platform used for identifying interactions of hundreds of proteins in parallel, as well as determining their functions. (edu.au)
  • Following a washing step to remove unbound secondary ligand, the extent to which a chromogenic substrate (e.g. 3,3', 5,5' tetramethyl benzidine chromogen [TMB]) is converted to a soluble coloured product by the conjugated enzyme in a given time is determined by spectrophotometry using a standard microplate absorbance reader. (ebi.ac.uk)
  • After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC 50 ) 33.6 ± 2.5 ng/mL. (mdpi.com)
  • The discovery of novel anti-cancer drugs targeting anaplastic lymphoma kinase (ALK), an oncogenic tyrosine kinase, raises the need for in vitro assays suitable for screening compounds for ALK inhibition. (haematologica.org)
  • A simple protocol for using a LDH-based cytotoxicity assay to assess the effects of death and growth inhibition at the same time. (edu.au)
  • Although the fluorescent-antibody-to-membrane-antigen (FAMA) test remains the reference standard to which other tests are compared, simpler alternative assays are needed. (eurekamag.com)
  • The assay has been validated for application to peanut butter, and demonstrates many advantages over conventional methodology, including the requirement for minimal sample preparation before assay, its technical simplicity, and its potential high sample through-put. (westminster.ac.uk)