OrganismsChemicals and Drugs
HyaluronoglucosaminidaseHyaluronic AcidChamomile
RecombinantSuperoxide dismutaseFillersExtracellular matrixCollagenaseFibrin clotsDarzalex FasproInjectionsDegradesPH20PhospholipaseTissuesFillerReactionPermeabilityVialInhibitorNanoparticlesBacterialMoleculesPlasminogenMolecularTumorSnakeEquivalentPatientsConcentrationAssayIncreasesAcidMiceAdultEffectEffectsDetermineBloodTreatmentAdditionFactorStudiesActivityControl
Recombinant11
  • Four different purified hyaluronidases have been approved for use in the United States, three of animal origin and one recombinant. (wikipedia.org)
  • Human recombinant hyaluronidase (Hylenex)-approved for use in the United States in December 2005-corresponds to the soluble fragment of human HYAL5 (PH20) produced in culture by genetically engineered Chinese hamster ovary cells containing a DNA plasmid encoding the enzyme. (wikipedia.org)
  • A human recombinant hyaluronidase kit, HyQvia, was approved for use in the European Union in May 2013, and in the United States in September 2014. (wikipedia.org)
  • It is a dual vial unit with one vial of immune globulin infusion 10% (human) and one vial of recombinant human hyaluronidase. (wikipedia.org)
  • It is an immune globulin with a recombinant human hyaluronidase indicated in the United States for the treatment of primary immunodeficiency in adults. (wikipedia.org)
  • Halozyme's proprietary ENHANZE ® drug-delivery technology is based on its patented recombinant human hyaluronidase enzyme (rHuPH20). (prnewswire.com)
  • Enhanze™ technology is a proprietary drug delivery platform using Halozyme's first approved enzyme, recombinant human hyaluronidase or rHuPH20. (prnewswire.com)
  • Using a recombinant DNA-derived hyaluronidase enzyme is an approach for possibly changing dosing regimens and bioavailability of several types of agents, including biologics. (pharmtech.com)
  • CheckMate -67T is a Phase 3 randomized, open-label trial evaluating subcutaneous administration of Opdivo co-formulated with Halozyme's proprietary recombinant human hyaluronidase, rHuPH20, or subcutaneous nivolumab (nivolumab and hyaluronidase) compared to intravenous (IV) Opdivo , in patients with advanced or metastatic clear cell renal cell carcinoma (ccRCC) who have received prior systemic therapy. (businesswire.com)
  • The CheckMate -67T noninferiority trial evaluated the subcutaneous formulation of Opdivo (nivolumab) co-formulated with Halozyme's proprietary recombinant human hyaluronidase (rHuPH20) compared to intravenous Opdivo in patients with advanced or metastatic clear cell renal cell carcinoma who have received prior systemic therapy. (pharmamanufacturing.com)
  • The technology is based on Halozyme's proprietary recombinant human hyaluronidase PH20 enzyme that degrades hyaluronan. (pharmamanufacturing.com)
Superoxide dismutase3
  • Molecular docking analysis for different enzymes involved in aging (collagenase, elastase and hyaluronidase), antioxidant enzymes (superoxide dismutase, glutathione-s-transferase, glutathione peroxidase and catalase) and mitochondrial enzymes (nicotinamide adenine dinucleotide (NAD)+hydrogen (H) dehydrogenase, succinate dehydrogenase, cytochrome c oxidase and adenosine triphosphate synthase) was carried out for epigallocatechin gallate alone (1), withaferin A alone (2), epigallocatechin gallate and withaferin A in combination (3) and a reference molecule. (ijpsonline.com)
  • 1, 3) Antioxidants, such as Coenzyme Q10, alpha lipoic acid and NADH (nicotinamide adenine dinucleotide) and anti-catabolic enzymes, such as glutathione peroxidase, superoxide dismutase and catalase minimize the damage due to ROS. (life-enthusiast.com)
  • either do not elicit or actively suppress the oxidative step, whereas other organisms produce enzymes (eg, catalase, glutathione reductase, superoxide dismutase) that mitigate the oxidative products. (msdmanuals.com)
Fillers6
  • Hyaluronic acid fillers, even product that has been in the face for years can be modified using the injectable enzyme Hyaluronidase. (lidlift.com)
  • Hyaluronidase can be injected to dissolve hyaluronic acid type dermal fillers and is the best treatment option for those looking at dissolving lip filler or dealing with related complications. (wikipedia.org)
  • This is a process that requires the use of an enzyme known as hyaluronidase to dissolve your fillers. (articlecity.com)
  • Hyaluronidase: Hyaluronidase is an enzyme that can dissolve hyaluronic acid fillers. (dollverse.com)
  • Hyaluronic acid fillers are easily correctable with injectible hyaluronidase. (ringpfeildermatology.com)
  • Hyaluronidase, an enzyme, can be used to reduce or remove HA fillers if undesired results occur. (vervelaser.com)
Extracellular matrix3
  • By catalyzing the hydrolysis of hyaluronan, a constituent of the extracellular matrix, hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. (wikipedia.org)
  • To determine whether the co-injection of extracellular matrix degrading enzymes improves retinal transduction following intravitreal delivery of adeno-associated virus-2 (AAV2). (molvis.org)
  • One hypothesis is that increased hyaluronidase may help prevent tumor invasion by breaking down the extracellular matrix needed for tumor expansion. (bioassaysys.com)
Collagenase1
  • AAV2 containing cDNA encoding enhanced green fluorescent protein (GFP), under the control of a chicken β-actin promoter, was delivered by intravitreal injection to adult mice in conjunction with enzymes including collagenase, hyaluronan lyase, heparinase III, or chondroitin ABC lyase. (molvis.org)
Fibrin clots1
Darzalex Faspro1
  • medical citation needed] Hyaluronidase is available in some fixed-dose combination drug products in the United States: rituximab/hyaluronidase (Rituxan Hycela), trastuzumab/hyaluronidase-oysk (Herceptin Hylecta), daratumumab/hyaluronidase-fihj (Darzalex Faspro), and pertuzumab/trastuzumab/hyaluronidase-zzxf (Phesgo). (wikipedia.org)
Injections4
  • The study found that injections of hyaluronidase reduced muscle stiffness in people with neurological injury without causing muscle weakness like conventional treatments such as botulinum toxin. (nyulangone.org)
  • Raghavan plans to begin a clinical trial to further study the effects of hyaluronidase in a greater number of patients, and adds that more research is needed to determine the effects of repeated hyaluronidase administrations overtime, although 15 of the 20 patients returned for additional injections. (nyulangone.org)
  • This case series provides preliminary evidence for the safety and potential efficacy of hyaluronidase injections as a treatment for muscle stiffness that may enhance recovery in the spastic upper limb. (nyulangone.org)
  • Here, we removed the ECM in the primary auditory cortex (ACx) of adult Mongolian gerbils using local injections of hyaluronidase (HYase). (nature.com)
Degrades1
PH201
  • The three naturally-sourced hyaluronidases are orthologs of human HYAL5 (PH20) obtained from testicular preparations. (wikipedia.org)
Phospholipase1
  • Additionally, two enzymes , hyaluronidase and phospholipase A2 (bvPLA2), together make up between 11 and 15 percent of bee venom. (howstuffworks.com)
Tissues5
  • HYAL1 and HYAL2 are the major hyaluronidases in most tissues. (wikipedia.org)
  • More than 20 different types of collagenases exist, but only a handful are most suited for the enzyme dissociation of tissues. (marketsandmarkets.com)
  • Comment: Salicylates, when given in large systemic doses, may render tissues partially resistant to the action of hyaluronidase. (medscape.com)
  • Another gram-positive bacterium, Clostridium perfringens produces hyaluronidases that are also thought to contribute to the spreading of bacteria in tissues (2). (swirlzcupcakes.com)
  • Definition of hyaluronidase : a mucolytic enzyme that facilitates the spread of fluids through tissues by lowering the viscosity of hyaluronic acid. (swirlzcupcakes.com)
Filler3
  • The enzyme only works on the hyaluronic acid filler in the face. (lidlift.com)
  • If you have persistent lumps or bumps, your provider may recommend using hyaluronidase to dissolve the filler. (dollverse.com)
  • Your practitioner can just inject an antidotal enzyme, called hyaluronidase, to dissolve the filler. (therapieclinic.com)
Reaction4
  • Karl Meyer classified these enzymes in 1971, into three distinct groups, a scheme based on the enzyme reaction products. (wikipedia.org)
  • Scientists have identified amines, enzymes and peptides that are responsible for the pain of - or allergic reaction to - a sting. (howstuffworks.com)
  • The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. (caslab.com)
  • BioAssay Systems' Hyaluronidase Inhibitor Screening Assay Kit uses a two-step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. (bioassaysys.com)
Permeability1
  • Hyaluronidase enhances permeability of tissue that boosts the rate of incorporation of other enzymes into the target's cells. (ukessays.com)
Vial1
  • One vial of hyaluronidase typically costs $50, and a single treatment may require 4 to 8 vials, while one vial of botulinum toxin may cost at least $500, and treatment may require up to 4 or more vials. (nyulangone.org)
Inhibitor1
  • I am using a different species or a different brand of hyaluronidase, how do I determine the optimal hyaluronidase concentration for inhibitor screening? (bioassaysys.com)
Nanoparticles1
  • Poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) enhance the delivery of therapeutic enzymes for replacement therapy of lysosomal storage disorders. (ibecbarcelona.eu)
Bacterial1
  • In addition, bacterial hyaluronate lyases (EC 4.2.2.1) may also be referred to as hyaluronidases, although this is uncommon. (wikipedia.org)
Molecules2
  • Enzyme input and addition of carrier protein were evaluated, rendering NPs having, e.g., 181 nm diameter, 0.15 PDI, −36 mV ζ potential, and 538 HAse molecules encapsulated per NP. (ibecbarcelona.eu)
  • Similar NPs were coated with enzyme, which reduced loading (e.g., 292 HAse molecules/NP). (ibecbarcelona.eu)
Plasminogen1
  • It is produced naturally to convert another blood protein, known as plasminogen, into an enzyme called plasmin. (swirlzcupcakes.com)
Molecular1
  • Therefore, the researchers believed that an enzyme that breaks down such molecular sugar chains, called hyaluronidase, may reduce their concentration in muscles to reduce stiffness and increase joint movement. (nyulangone.org)
Tumor1
  • Conversely, decreasing hyaluronidase activity might prevent metastasis by stopping cancer cells from escaping primary tumor masses. (bioassaysys.com)
Snake5
  • In this work, a general biochemical characterization in snake venom of serine proteases (SVSP), phospholipases A 2 (PLA 2 ), metalloproteases (SVMP) and hyaluronidases (SVH) of Crotalus aquilus (Ca), Crotalus polystictus (Cp) and Crotalus molossus nigrescens (Cmn) was done. (mdpi.com)
  • Hyaluronidase in snake and insect venom is thought to function as a "spreading factor" by degrading host hyaluronic acid, thus allowing spread of toxin (13). (swirlzcupcakes.com)
  • Snake venom is a mixture of different enzymes and proteins which many of it not harmless to humans, but some are very toxic. (ukessays.com)
  • Thrombin-like enzymes (SVTLE) snake venom is used for fibrinogen breakdown assay and for the fibrinogen dysfunction detection. (ukessays.com)
  • As mentioned, snake venom is modified saliva which contains a variety of proteins and enzymes. (ukessays.com)
Equivalent1
  • Patients may require larger amounts of hyaluronidase for equivalent dispersing effect. (medscape.com)
Patients1
  • Hyaluronidase may provide a relatively less expensive option for patients with spasticity, according to Raghavan. (nyulangone.org)
Concentration2
  • 1. Prepare stock Hyaluronidase solution in Enzyme Buffer (start at a high concentration, you can always dilute down). (bioassaysys.com)
  • Select a concentration of enzyme that yields an OD600nm reading about half way between the NEC and NSC OD600nm readings. (bioassaysys.com)
Assay1
  • Inhibition readouts that are significantly negative may mean there is a substance interfering with the assay or that the test compound is acting as an activator to improve the enzyme activity rather than inhibit it. (bioassaysys.com)
Increases1
  • Comment: Hyaluronidase hastens the onset of local analgesia and reduces swelling, but increases systemic absorption of anesthetic. (medscape.com)
Acid5
Mice1
  • NPs were internalized, trafficked to lysosomes, released active enzyme at lysosomal conditions, and targeted both peripheral organs and the brain after i.v. administration in mice. (ibecbarcelona.eu)
Adult1
  • Here, we acutely weakened the ECM in the primary auditory cortex (ACx) of adult Mongolian gerbils ( Meriones unguiculatus ) by local microinjections of hyaluronidase (HYase). (nature.com)
Effect2
  • Hyaluronidase took effect within days to two weeks, with effects that lasted for at least three months. (nyulangone.org)
  • beclomethasone, inhaled will decrease the level or effect of hyaluronidase by unspecified interaction mechanism. (medscape.com)
Effects2
  • aspirin decreases effects of hyaluronidase by Other (see comment). (medscape.com)
  • Overall, the results indicate that epigallocatechin gallate and withaferin A, in combination, may potentially inhibit skin-aging, while enhancing antioxidant effects of various enzymes, and warrant further experimental validation. (ijpsonline.com)
Determine1
  • The study to determine hyaluronidases' exact role in cancer pathology is still ongoing. (bioassaysys.com)
Blood1
  • Hyaluronidase dilates blood vessels, triggering inflammation throughout the body. (howstuffworks.com)
Treatment3
  • A naturally occurring enzyme called hyaluronidase may be an effective alternative treatment for spasticity, or muscle stiffness, a disabling condition in people who have had a stroke or other brain injury. (nyulangone.org)
  • This enzyme is also beneficial to correct an issue that occurred during a lip treatment. (articlecity.com)
  • The role of hyaluronidases in breaking down this key factor in cell proliferation makes them a possible target for cancer treatment. (bioassaysys.com)
Addition1
  • Without the addition of enzymes, transduction was limited to occasional cells in the retinal ganglion cell layer. (molvis.org)
Factor1
Studies1
  • Previous studies examined NPs encapsulating or coated with enzymes, but these formulations have never been compared. (ibecbarcelona.eu)
Activity1
  • The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample. (bioassaysys.com)
Control1
  • 3. Transfer 40 µL of dH2O into two separate wells for the No Enzyme Control (NEC) and No Substrate Control (NSC). (bioassaysys.com)