Loading...
  • immunoassay
  • 2. The enzyme immunoassay of claim 1, wherein the binding material is immunologically immobilized within a finite zone of the inert, porous medium. (google.com)
  • 3. The enzyme immunoassay of claim 1, wherein the binding material is an antibody specific for said analyte and is immunologically immobilized within said zone by applying a solution of said antibody to said zone and applying an antiserum to said antibody to said zone, thus effecting an immunological reaction in said zone. (google.com)
  • 5. The enzyme immunoassay of claim 1, wherein the analyte is a therapeutic drug and the binding material is an antibody specific for said therapeutic drug. (google.com)
  • In vitro assays using human serum as a source of complement were used, and the C3 split product iC3b was measured by enzyme immunoassay. (arvojournals.org)
  • The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the. (google.co.uk)
  • wherein
  • 2. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of indicator compounds and dyes. (google.com)
  • 2. The method of claim 1 wherein the contacting step (c) comprises directly contacting the liquid sample with the enzyme-labelled immunologically active compound specifically bound to the specific binding partner on the porous solid phase to form the first liquid phase thereon. (google.co.uk)
  • 7. The method of claim 1 wherein the enzyme-labelled immunologically active compound of step (b) is provided as a separate liquid reagent. (google.co.uk)
  • 11. The method of claim 1 wherein the contacting step (c) comprises first contacting the liquid sample with the enzyme-labelled immunologically active compound provided as a separate liquid reagent to form a first liquid phase, and contacting the first liquid phase with the porous solid phase. (google.co.uk)
  • proteins
  • More specifically, the present invention relates to a method for the identification of compounds capable of binding and/or inhibiting cyclophilin-like proteins, as well as to methods of treating parasitic infections which are not susceptible to cyclosporin A. (google.com)
  • This enzyme catalyzes the cis-trans isomerization of proline-imidic peptide bonds in oligopeptides and has been demonstrated to accelerate the refolding of several proteins, including collagens [Bachinger, J. Biol. (google.com)
  • bound
  • Measuring the population of enzymes whose active site was occupied by a polymer chain revealed that Cel7A was bound predominantly via its active site. (embl-heidelberg.de)
  • strains
  • The reaction is confined to organisms producing β-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in strains previously considered not to produce it. (asm.org)
  • antibody
  • Both AP and HRP can be used to detect protein or antigens via an indirect or direct antibody detection test, where the enzyme is conjugated to the primary antibody which then binds to the target antigen or a secondary antibody that attaches to the primary antibody. (squarespace.com)
  • biochemical
  • In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. (google.com)
  • Despite the amount of research conducted on FlgJ from these and other bacteria over the past 15 years, no biochemical analysis had been conducted on any FlgJ and consequently confusion exists as to whether the enzyme is a peptidoglycan hydrolase or a lytic transglycosylase. (embl-heidelberg.de)
  • system
  • thus making the enzyme system very versatile and easy to use. (squarespace.com)
  • The complement system is activated through three enzyme cascades, the classical, lectin, and alternative pathways, which converge at the step involving cleavage of C3 to C3a and C3b. (arvojournals.org)
  • Drugs
  • The stably tethered structures may bind to and/or be conjugated to a variety of known effectors, such as drugs, enzymes, radionuclides, therapeutic agents and/or diagnostic agents. (patentsencyclopedia.com)
  • bind
  • The chemical similarity of cellulose and chitin supports the idea that their corresponding hydrolytic enzymes would bind beta-1,4-linked glucose residues in a similar manner. (embl-heidelberg.de)
  • The invention also relates to recombinant methods for producing the polypeptides and methods for identifying compounds that bind to and/or modulate the activity of the polypeptides. (google.com)
  • activity
  • Compounds that interact with and stimulate the activity of CANNABINOID RECEPTORS. (nih.gov)
  • Enzymes are pivotal elements in bioanalysis due to their specificity and extremely high catalytic activity. (rsc.org)
  • Here we report on a novel technique that can be used to simultaneously measure activity levels for a panel of enzymes, such as the MMPs. (duke.edu)
  • fragment
  • Complexing of C3b with the Bb fragment of factor B generates the C3 cleavage enzyme C3bBb, which, by further C3 cleavage, establishes the complement amplification loop. (arvojournals.org)
  • diseases
  • Matrix metalloproteinases (MMPs) are a family of enzymes that are up-regulated in many diseases, including osteoarthritis (OA) and rheumatoid arthritis (RA). (duke.edu)
  • results
  • Finally because of the high turnover of the enzymes, this also results in more enzyme binding to the protein and the signal emission can be deleted for a long time. (squarespace.com)
  • variety
  • HRP readily combines with hydrogen peroxide (H 2 O 2 ) and the resultant [HRP-H 2 O 2 ] complex can oxidize a wide variety of chromogenic hydrogen donors. (sigmaaldrich.com)