C23OCytochrome P-450Extradiol dioxygenaseBacteriaInhibitorsSubstratesCleavageCharacterizationCatalyzesOxygenasesPurified to homogeneityRhodococcusOxidationMuconic acidGeneMechanismSurfactantGenesDegradationManganeseReactionLigandTyrosinaseGlyceraldehyde-3-phosphate dEscherichiaExtractsHydrophobicHomologyCleaveProteinsAtomsSynthesisBiosynthesisDifferentiationProteinBRENDAVirtuallyTemperatureEndogenousMolecularFattyToxicFamiliesActiveFamily
C23O3
- The efficiency and regulatory properties of p ugp were measured using xylE and lacZ as reporter genes, which code for the corresponding enzymes catechol-2,3-dioxygenase (C23O) and β-galactosidase (βGal), respectively. (nau.edu)
- Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
- The existence of catabolic and surfactant genes namely catechol dioxygenase ( C23O ), rhamnolipid enzyme ( rhlB ) and surfactin /lichenysin enzyme ( SrfA3 /LicA3 ) genes were detected in only four (4) out of the nine (9) marine aromatic degrading bacteria with 881 base pairs sizes. (journalarrb.com)
Cytochrome P-4503
- Cytochrome P-450 Enzyme System" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
- This graph shows the total number of publications written about "Cytochrome P-450 Enzyme System" by people in this website by year, and whether "Cytochrome P-450 Enzyme System" was a major or minor topic of these publications. (wakehealth.edu)
- Below are the most recent publications written about "Cytochrome P-450 Enzyme System" by people in Profiles. (wakehealth.edu)
Extradiol dioxygenase2
- The two enzymes were identified to be a part of two separate catechol dioxygenase families: 1,2-CTD was classified as an intradiol dioxygenase while 2,3-CTD was classified as an extradiol dioxygenase. (wikipedia.org)
- It exhibited a half life of 30 min at 80 C and 81 min at 75 C, making it the most thermostable extradiol dioxygenase studied. (muni.cz)
Bacteria6
- Thus far, 1,2-CTD has been observed to exist in the following species of soil bacteria and fungi: Pseudomonas sp. (wikipedia.org)
- These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
- Enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelactone. (inrae.fr)
- The root-mean-square deviation from ideal bond distances for the model is 0.014 A and the error in atomic co-ordinates is estimated to be 0.15 A. DLH is a monomeric enzyme containing 236 amino acid residues and is a member of the beta-ketoadipate pathway found in bacteria and fungi. (inrae.fr)
- A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)
- Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)
Inhibitors3
- The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
- Substrates containing a -COOH group are inhibitors for catechol oxidase. (lookformedical.com)
- In vitro production of enzymatically active O. volvulus prolyl 4-hydroxylase should facilitate identification of specific inhibitors of the parasite enzyme. (embl.de)
Substrates4
- The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
- Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
- In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
- 2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). (wakehealth.edu)
Cleavage3
- A novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC_JF8) catalyzing the meta-cleavage of the hydroxylated biphenyl ring was purified from the thermophilic biphenyl and naphthalene degrader, Bacillus sp. (muni.cz)
- Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
- In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)
Characterization3
- Cloning and characterization of a novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB and naphthalene degrading Bacillus sp. (muni.cz)
- 1. Fischer, D., Ebenau-Jehle, C. and Grisebach, H. Phytoalexin synthesis in soybean: purification and characterization of NADPH:2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell cultures. (qmul.ac.uk)
- Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
Catalyzes7
- Dienelactone hydrolase (DLH), an enzyme from the beta-ketoadipate pathway, catalyzes the hydrolysis of dienelactone to maleylacetate. (inrae.fr)
- An enzyme that catalyzes the hydroxylation of TRYPTOPHAN to 5-HYDROXYTRYPTOPHAN in the presence of NADPH and molecular oxygen. (harvard.edu)
- An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. (nih.gov)
- An enzyme that catalyzes the oxidation of BENZOATE to 4-hydroxybenzoate. (uchicago.edu)
- An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. (lookformedical.com)
- The enzyme catalyzes the oxidation of o-diphenols to o-quinones without hydroxylating monophenols, the additional activity of tyrosinase. (lookformedical.com)
- A member of the P450 superfamily, this enzyme catalyzes the first oxidative step of the phenylpropanoid pathway in higher PLANTS by transforming trans-cinnamate into p-coumarate. (bvsalud.org)
Oxygenases2
- Oxygenases can further be grouped into two categories i.e. monooxygenases and dioxygenases on the basis of number of oxygen atoms used for oxidation. (biomedcentral.com)
- At present the database contains information of over 235 oxygenases including both dioxygenases and monooxygenases. (biomedcentral.com)
Purified to homogeneity2
- The native and recombinant BphC enzyme was purified to homogeneity. (muni.cz)
- Prophenoloxidase-activating enzyme (PPAE) was purified to homogeneity as judged by SDS-polyacrylamide gel electrophoresis from larval cuticles of the silkworm, Bombyx mori. (lookformedical.com)
Rhodococcus1
- An integrated approach of bioassay and molecular docking to study the dihydroxylation mechanism of pyrene by naphthalene dioxygenase in Rhodococcus sp. (brenda-enzymes.org)
Oxidation1
- Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp. (afpm.org.my)
Muconic acid1
- 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
Gene6
- When the xylE gene was maximally expressed, the induced enzyme constituted approx. (nau.edu)
- 6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
- Each enzyme entry contains its common name and synonym, reaction in which enzyme is involved, family and subfamily, structure and gene link and literature citation. (biomedcentral.com)
- For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
- Non-mammalian fat-1 gene prevents neoplasia when introduced to a mouse hepatocarcinogenesis model: Omega-3 fatty acids prevent liver neoplasia. (ouhsc.edu)
- For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism. (wakehealth.edu)
Mechanism2
- The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
- New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
Surfactant1
- This nanocomposite was prepared with slight modification of nanocrystalline cellulose (NCC) with cationic surfactant of cetyltriammonium bromide (CTAB) and further decorated with 3-mercaptopropionic acid (3-MPA) capped CdS QDs. (afpm.org.my)
Genes2
- The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)
- B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)
Degradation1
- Further, the centralized degradation pathways mean synthesis of fewer degradative enzymes requiring less metabolic energy. (biomedcentral.com)
Manganese1
- ICP-MS analysis confirmed the presence of 4.0 to 4.8 manganese atoms per enzyme molecule. (muni.cz)
Reaction2
- The two enzymes can be distinguished based on their reaction products and cofactors. (wikipedia.org)
- In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. (qmul.ac.uk)
Ligand1
- The second catechol hydroxyl group on carbon 3 (C3) is coordinated to Fe3+ after its deprotonation by the Tyr200 ligand. (wikipedia.org)
Tyrosinase1
- The nanocomposite material was then employed as scaffold for immobilization of tyrosinase enzyme (Tyr). (afpm.org.my)
Glyceraldehyde-3-phosphate d1
- Glyceraldehyde 3-phosphate dehydrogenase [Interproscan]. (ntu.edu.sg)
Escherichia3
- The ugp promoter (p ugp ) responsible for expression of the binding-protein-dependent sn-glycerol-3-phosphate transport system in Escherichia coli was cloned into a small multicopy plasmid pTER5, a derivative of pBR322, between the transcription terminators rpoCt and t L1 . (nau.edu)
- protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
- protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
Extracts2
- The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. (afpm.org.my)
- Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. (shengsci.com)
Hydrophobic1
- As the thiolate anion is not energetically favoured in the hydrophobic interior of the enzyme, it swings into the active site where it can be stabilized by the His202 imidazolium and the dipole of helix C. The Cys123 thiolate which now lies adjacent to the acyl carbon of the substrate, is thus generated only in the presence of substrate. (inrae.fr)
Homology1
- A 3-D homology model of BphC_JF8 provided a basis for understanding the substrate specificity, quaternary structure and stability of the enzyme. (muni.cz)
Cleave1
- In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
Proteins1
- It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. (lookformedical.com)
Atoms1
- The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
Synthesis1
- 1,2-CTD degrades phenolic hydrocarbons key to the synthesis lipid membranes. (wikipedia.org)
Biosynthesis2
- A survey of agricultural technologies influencing the biosynthesis and accumulation of phenolic compounds in crop plants is presented, including observations on the effects of light, temperature, mineral nutrition, water management, grafting, elevated atmospheric CO 2 , growth and differentiation of the plant and application of elicitors, stimulating agents and plant activators. (mdpi.com)
- The enzyme responsible for collagen maturation is prolyl 4-hydroxylase, making this enzyme a central activity in cuticle biosynthesis and a potentially important chemotherapeutic target. (embl.de)
Differentiation1
- Ran GTP benefits with differentiation, acquiring its enzyme. (evakoch.com)
Protein2
- This competitive enzyme-linked immunosorbent assay-like method, used competitive binding of free hyaluronan in the sample and biotin-labeled standard hyaluronan to hyaluronan binding protein in solid phase. (go.jp)
- Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
BRENDA1
- The entries are also linked to several external database including BRENDA, KEGG, ENZYME and UM-BBD providing wide background information. (biomedcentral.com)
Virtually2
- Enzyme activities were virtually completely repressed in the presencee of excess inorganic phosphates (P i ) and high concentrations of glucose. (nau.edu)
- It has not been observed in other hydrolytic enzymes and it is virtually inactive when it is produced by site-directed mutagenesis in the proteases. (inrae.fr)
Temperature2
- A decrease in Km accompanied an increase in temperature and the Km value of 0.095 uM for 2,3-dihydroxybiphenyl (at 60 C) is among the lowest reported. (muni.cz)
- Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
Endogenous1
- Endogenous n-3 polyunsaturated fatty acids delay progression of pancreatic ductal adenocarcinoma in Fat-1-p48(Cre/+)-LSL-Kras(G12D/+) mice. (ouhsc.edu)
Molecular1
- The enzyme has a molecular mass of 125+-10 kDa and was composed of 4 identical subunits (35 kDa). (muni.cz)
Fatty1
- Maternal single nucleotide polymorphisms in the fatty acid desaturase 1 and 2 coding regions modify the impact of prenatal supplementation with DHA on birth weight. (ouhsc.edu)
Toxic1
- Many of these compounds have been reported to be toxic to the living organisms [ 3 ]. (biomedcentral.com)
Families1
- Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and catechol 2,3-dioxygenase (2,3-CTD). (wikipedia.org)
Active1
- The first is that the binding of the terminal phospholipids alters the conformation of the active sites, implying that the tunnel acts as an effector, only allowing the enzyme to be active in certain areas of the cell. (wikipedia.org)
Family1
- A family of enzymes that catalyze the stereoselective, regioselective, or chemoselective syn-dehydrogenation reactions. (ouhsc.edu)