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  • reaction
  • The optimum pH for an enzymatic reaction may shift by as much as 3 pH units upon immobilization.24 This shift is a result of both the charge of the support material and the chemical modification of the enzyme. (clicktocurecancer.info)
  • The reaction of 1 and catechol in the presence of excess triethylamine gave the catecholate (CAT) chelate b is(u -al koxo) -bridged dinuclear iron(Ill) complex [Fe(L)(CAT)12 (2). (diva-portal.org)
  • An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, shown below. (wikipedia.org)
  • If you click on the box you will change to the appropriate reaction scheme or enzyme specification. (qmul.ac.uk)
  • 3-9 Although the reaction with dioxygen is thermodynamically favourable, the electronic configuration of the O 2 molecule creates a strong kinetic barrier that requires activation, which is useful to prevent any spontaneous reaction of O 2 with organic molecules. (rsc.org)
  • In enzymology, a lactoylglutathione lyase (EC 4.4.1.5) (also known as glyoxalase I) is an enzyme that catalyzes the isomerization of hemithioacetal adducts, which are formed in a spontaneous reaction between a glutathionyl group and aldehydes such as methylglyoxal. (wikipedia.org)
  • For example, in the α-ketoglutarate-dependent enzymes, one atom of dioxygen is incorporated into two substrates, with one always being α-ketoglutarate, and this reaction is brought about by a mononuclear iron center. (wikipedia.org)
  • The two enzymes can be distinguished based on their reaction products and cofactors. (wikipedia.org)
  • The oxidation of the catechol 3',4'-dihydroxyl electron-donating groups occurs first, at very low positive potentials, and is a reversible reaction. (wikipedia.org)
  • oxygenase
  • 2. Role of oxygen in the metabolic regulation : Besides the well known … More role of oxygen in the energy metabolism, oxygen has been shown to play an important physiological role in metabolic regulation through oxygenase-catalyzed reactions. (nii.ac.jp)
  • For the elucidation of the active site structure of another well characterized oxygenase, catechol 2, 3 - dioxygense, we used o-nitrophenol, a competitive inhibitor, and found it a useful active site probe for this enzyme. (nii.ac.jp)
  • nonheme
  • His inorganic chemistry research group at the University of Minnesota focuses on iron chemistry relevant to biocatalysis, in an attempt to better understand oxygen activation mechanisms of nonheme iron enzymes. (wikipedia.org)
  • gene
  • We have determined the nucleotide (nt) sequence of the dmpB structural gene for this enzyme, and expressed and identified its polypeptide product in Escherichia coli. (diva-portal.org)
  • Structurally, the enzyme is a domain-swapped dimer in many species, although the two subunits have merged into a monomer in yeast, through gene duplication. (wikipedia.org)
  • The human gene for this enzyme is called GLO1. (wikipedia.org)
  • aromatic compounds
  • Six species of free-living nitrogen fixing bacteria, Azomonas agilis, Azospirillum brasilense, Azospirillum lipoferum, Azotobacter chroococcum, Azotobacter vinelandii , and Beijerinckia mobilis , were surveyed for their ability to grow and fix N 2 using aromatic compounds as sole carbon and energy source. (springer.com)
  • Dorn E, Knackmuss H-J (1978) Chemical structure and biodegradability of halogenated aromatic compounds: two catechol 1,2-dioxygenases from a 3-chlorobenzoate grown pseudomonad. (springer.com)
  • iron enzymes
  • His group is also working towards designing functional models for iron enzymes and capturing, observing, and categorizing highly active metal-based intermediates. (wikipedia.org)
  • intradiol
  • However, the enzyme preparations from Brevibacterium and Arthrobacter have only the intradiol cleavage activity. (wikipedia.org)
  • HL = NN-bis(2-pyridylmethyl)-N-(2hydroxyethyl)amine] of the tripodal N3O ligand was prepared as a biomimetic model for the intradiol-cleaving dioxygenase enzymes. (diva-portal.org)
  • Complex 1 displays the intradiol-cleaving dioxygenase activity, and the coordinate ethoxyl arm of the ligand is capable of accepting the proton from catechol, which mimics the function of Tyr447 in the. (diva-portal.org)
  • Intradiol enzymes cleave the carbon-carbon bond between the two hydroxyl groups. (wikipedia.org)
  • NADH
  • The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate and NAD+ to produce 3-methylcatechol, NADH and CO2. (wikipedia.org)
  • phenol
  • Catechol is produced industrially by the hydroxylation of phenol using hydrogen peroxide. (wikipedia.org)
  • Catechin /ˈkætɪtʃɪn/ is a flavan-3-ol, a type of natural phenol and antioxidant. (wikipedia.org)
  • catalytic
  • Particle size is also an important consideration: smaller particles have been observed to yield catalytic properties more closely approximating those obtained with soluble enzymes. (clicktocurecancer.info)
  • Catalytic oxidation of 3,5-di- t -butyl-catechol (3,5-DTBC) into quinone occurred without the need of an additional base when the metal complex was confined in the porous solid. (rsc.org)
  • 6-9 In particular, the manganese-dependent dioxygenase (MndD) is a system that has attracted much attention since its catalytic mechanism is still under discussion. (rsc.org)
  • The most widely observed cofactor involved in dioxygenation reactions is iron, but the catalytic scheme employed by these iron-containing enzymes is highly diverse. (wikipedia.org)
  • The enzyme resembles a boomerang in shape, and can therefore be clearly divided into three domains: two catalytic domains residing at each end of the "boomerang" and a linker domain at the center. (wikipedia.org)
  • The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
  • oxidation
  • In comparision, the oxidation of 4- t -butyl-catechol (4-TBC) that always required a basic media led to a total oxidation into the ortho -quinone, contrary to the molecular analogue. (rsc.org)
  • For instance, carbon-carbon bond cleavage, fatty acid hydroperoxidation, carbon-sulfur bond cleavage, and thiol oxidation are all reactions catalyzed by mononuclear iron dioxygenases. (wikipedia.org)
  • The ability to quench singlet oxygen seems to be in relation with the chemical structure of catechin, with the presence of the catechol moiety on ring B and the presence of a hydroxyl group activating the double bond on ring C. Electrochemical experiments show that (+)-catechin oxidation mechanism proceeds in sequential steps, related with the catechol and resorcinol groups and the oxidation is pH-dependent. (wikipedia.org)
  • Chemical oxidation of caffeic acid in acidic conditions using sodium periodate leads to the formation of dimers with a furan structure (isomers of 2,5-(3′,4′-dihydroxyphenyl)tetrahydrofuran 3,4-dicarboxylic acid). (wikipedia.org)
  • biosynthesis
  • Recently, oxygenases have been shown to be involved in the activation of peptide hormones in mammals and in the biosynthesis of nitric oxide (NO), an endothelium derived relaxation factor, therefore, much attention has been paid to this type of enzymes from the stand point of signal transduction in the cells. (nii.ac.jp)
  • caffeate
  • The transformation to ferulic acid is catalyzed by the enzyme caffeate O-methyltransferase. (wikipedia.org)
  • Dihydroxyphenylalanine ammonia-lyase was presumed to use 3,4-dihydroxy-L-phenylalanine (L-DOPA) to produce trans-caffeate and NH3. (wikipedia.org)
  • Caffeate O-methyltransferase is an enzyme responsible for the transformation of caffeic acid into ferulic acid. (wikipedia.org)
  • Caffeate 3,4-dioxygenase is an enzyme that uses caffeic acid and oxygen to produce 3-(2-carboxyethenyl)-cis,cis-muconate. (wikipedia.org)
  • bacteria
  • 3. The composition of claim 1 wherein the host organism comprises bacteria of the Bacillus or Clostridia genera. (patents.com)
  • Catechols such as DHSA are produced through the metabolism of cholesterol by bacteria such as Mycobacterium tuberculosis. (wikipedia.org)
  • nitrogen
  • strain JS42 is able to utilize 2-nitrotoluene (2NT) as its sole carbon, nitrogen, and energy source. (nih.gov)
  • All heteroatoms (N3O) of the ligand are coordinated to the iron center in complex 1 with two pyridine nitrogen atoms on the axial bonds, while one of the pyridyl arms of the ligand is left uncoordinated in complex 2. (diva-portal.org)
  • plasmid
  • The genes coding for these enzymes are located on pVI150, an IncP-2 degradative mega plasmid of this strain. (diva-portal.org)
  • Restriction enzyme digest fragment patterns of plasmid DNA generated with Xhol showed isolates 7/167 and 8/46 contain different sized plasmids, 61 kb and 95 kb, respectively. (waikato.ac.nz)
  • Assays
  • This hypothesis was supported by the results from enzyme assays of the crude cell extract of the consortium revealing catechol 1,2-dioxygenase activity which converted catechol and 4-chlorocatechol to cis,cis-muconic acid and 3-chloro-cis,cis-muconic acid respectively. (kent.ac.uk)