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  • Chapter
  • Chapters 1 and 2 of this dissertation analyzes the structure and function of the Mn-dependent enzyme oxalate decarboxylase (OxDc) and Chapter 2 presents a bioinformatic analysis of the cupin superfamily that provides the structural scaffold of the decarboxylase. (bu.edu)
  • Chapter_11_Catalysis - Chemistry 233 Fundamentals of. (coursehero.com)
  • hydrolysis
  • Water present in the organic solvent at a concentration of about 0.03% to about 2.5% is sufficient to enhance the rate of catalysis and stabilize the enzyme without substantially increasing the rate of hydrolysis when compared to the anhydrous enzyme. (uiowa.edu)
  • carbonyl
  • A difference spectrum in which 13C-substituted acyl-enzyme was subtracted from [12C]acyl-enzyme shows two bands in the ester carbonyl region and thus confirms the assignment of the features to the single ester carbonyl group. (biochemj.org)
  • In each case a lower-frequency relatively narrow band is assigned to a productive potentially reactive binding mode in which the carbonyl oxygen atom is inserted in the oxyanion hole of the enzyme active centre. (biochemj.org)
  • Measurements of carbonyl absorption frequencies in a number of solvents of the methyl esters of the acyl groups used to make acyl-enzymes have permitted determination of the apparent dielectric constants experienced by carbonyl groups in the enzyme active centre as well as a discussion of the effects of polarity. (biochemj.org)
  • Synthesis
  • The first comprehensive coverage of this unique and interdisciplinary field provides a complete overview, covering such topics as chemoenzymatic synthesis, microbial production of DNA building blocks, asymmetric transformations by coupled enzymes and much more. (wiley.com)
  • His research interests concentrate on molecular biology applied to organic synthesis and enzyme catalysis. (wiley.com)
  • fluctuations
  • We generated a mutant of Escherichia coli dihydrofolate reductase that abrogates millisecond-time-scale fluctuations in the enzyme active site without perturbing its structural and electrostatic preorganization. (sciencemag.org)
  • proteins
  • Professor Mulholland said: "As our research indicates that this type of interaction is likely to be important in other enzymes, it could ultimately help in the design of new proteins with applications in medicine and bioengineering. (phys.org)
  • OxDc is a member of the cupin superfamily, which comprises a wide variety of proteins and enzymes with great sequence and functional diversity. (bu.edu)
  • Enzymes are proteins produced by living cells. (nation2.com)
  • chemistry
  • Using molecular simulations, Dr Kara Ranaghan and Professor Adrian Mulholland from Bristol's School of Chemistry found an interesting interaction involving a particular methionine residue in the enzyme phosphite dehydrogenase. (phys.org)
  • conformational
  • In a collaboration with the group of Dr Colin Jackson, we are using a combination of theory and experiment to study the origin of enzyme catalysis and particularly the role of tunneling, conformational effects and electrostatic effects. (edu.au)
  • organic
  • Enzyme catalysis in organic solutions containing water" by Jonathan S. Dordick, Vikram M. Paradkar et al. (uiowa.edu)
  • A method is presented for catalyzing the conversion of substrate into product in an organic reaction solvent with an enzyme-surfactant ion pair. (uiowa.edu)
  • aspects
  • In particular, and with hindsight, our discussions might have been enriched by more detailed coverage of general aspects of chemical catalysis - but readers requiring this background should find adequate references herein. (springer.com)
  • remarkable
  • Another remarkable instance of entropic effects is found in enzymes that are adapted by evolution to work at low temperatures, near the freezing point of water. (diva-portal.org)
  • approaches
  • http://www.thomasyoungcentre.org/events/interface-between-experimental-and-theoretical-approaches-to-energy-related-enzyme-catalysis/ Contact: Dr. Jochen Blumberger (j.blumberger .at. (ccl.net)
  • Role
  • A multi-institutional research team led by Chris Dealwis from Case Western Reserve University has used the new IMAGINE instrument at Oak Ridge National Laboratory s (ORNL s) High Flux Isotope Reactor (HFIR) to map an enzyme that could play an important role in anti-cancer drug development. (nanowerk.com)
  • molecules
  • The team essentially watched the enzyme work its magic, binding its substrate and preparing the active site for catalysis by protonating specific atoms and correctly orienting water molecules. (nanowerk.com)
  • investigate
  • We seek to investigate the mechanism of single enzyme catalysis by state of the art electrochemical techniques, which includes low noise electronic systems that is sufficient enough to enable detecting electronic signal above the noise level, arising from a single enzyme. (ucsb.edu)
  • Structural
  • This protonation is proposed to occur directly via water, with the proton s access to the substrate regulated by the structural fluctuation of the enzyme. (nanowerk.com)
  • Understanding
  • For scientists, understanding details of how enzymes work is essential to the discovery of drugs to cure diseases and treat disorders. (nanowerk.com)
  • electrostatic
  • Low electrostatic stabilization in aqueous solutions is shown to be consistent with surprisingly large electrostatic stabilization effects in active sites of enzymes. (pnas.org)
  • experimental
  • However, despite substantial efforts from both the experimental and theoretical side, no real consensus has been reached regarding the origin of such large entropic contributions to enzyme catalysis. (diva-portal.org)
  • accord
  • It was not our intention at this meeting to present a complete up-to-the-minute review of current research in enzyme catalysis but t·ather, in accord wi th the intended spiri t of NATO ASis, to gi ve an opportunity for advanced students and researchers in a wide variety of disciplines to meet tagether and study the problern from different points of view. (springer.com)
  • rate
  • These experiments showed that replacing the methionine slowed down the rate of the reaction without significantly changing other properties of the enzyme. (phys.org)
  • active site
  • Using neutron crystallography, the team unequivocally mapped the active site of the enzyme dihydrofolate reductase (DHFR), an enzyme necessary for nucleotide biosynthesis and a classical drug target. (nanowerk.com)