Enzyme activity in l1210 leukemia cells. Medical search. Frequent questions

These findings indicate that nonfunctional dihydrofolate reductase in human leukemia cells share an antigenic determinant(s) with a functional form of the enzyme from L1210 murine leukemia cells. (aku.edu)
We have observed that DAC reduced the in vitro clonogenic potential in a dose-dependent manner of both human and murine leukemia and tumor cell lines. (biomedcentral.com)

In this study, we investigated the effects of Accepted 14 September 2009 nine naturally occurring compounds isolated from Citrus jambhiri Lush and Citrus pyriformis Hassk Available online 24 September 2009 (Rutaceae) for their potential to modulate the activity of P-gp in the multidrug-resistant human leukaemiacell line CEM/ADR5000. (gotomydoctor.com)

In particular, some congeners of this drug family (for example those that contain the C-8 fluorine), display high activity not only against bacterial topoisomerases, but also against eukaryotic topoisomerases and are toxic to cultured mammalian cells and in vivo tumor models. (wikipedia.org)
NK activation was evaluated, and its tumoricidal activity was assessed using in vitro and in vivo tumor models. (weeksmd.com)
In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
Tumor cell resistance to anthracyclines and epipodophyllotoxins can be due to reduced drug accumulation and/or alterations in the activity of topoisomerase II (TOPO II). (ucy.ac.cy)
However, relevant research has ebbed due to the limitation of these agents, which is the easy acquisition of tumor resistance by de novo glutamine synthesis in cancer or stromal cells. (encyclopedia.pub)
We found that MMDX cytotoxicity for cultured M5076 tumor cells was potentiated 22-fold by preincubating the drug with NADPH-supplemented liver microsomes from untreated C57BL/6 female mice. (unboundmedicine.com)
Clonogenic assays were performed on leukemic and tumor cell lines to evaluate the in vitro antineoplastic activity of DAC. (biomedcentral.com)
The in vivo antineoplastic activity of DAC administered as an i.v. infusion was evaluated in mice with murine L1210 leukemia by measurement of survival time, and in mice bearing murine EMT6 mammary tumor by excision of tumor after chemotherapy for an in vitro clonogenic assay. (biomedcentral.com)
Increasing the DAC concentration and duration of exposure produced a greater loss of clonogenicity for both human leukemic and tumor cell lines. (biomedcentral.com)
We also observed a correlation between in vitro and in vivo concentrations needed to produce optimal responses in mice with L1210 leukemia or with EMT6 tumor. (biomedcentral.com)
M.S. Webb, N.L. Boman, D. Masin, D. Yapp, E. Ramsay, P.R. Cullis and M.B. Bally, "A Cationic Liposomal Vincristine Formulation with Improved Vincristine Retention, Extended Circulation Lifetime and Increased Anti-Tumor Activity", Biochim. (ubc.ca)
2007). Encapsulation in liposomal nanoparticles enhances the immunostimulatory, adjuvant and anti-tumor activity of subcutaneously administered CpG ODN. (ubc.ca)

Antiserum raised in chickens to dihydrofolate reductase purified from L1210 leukemia cells by affinity chromatography inhibited the catalytic activity and the binding of methotrexate by the enzyme. (aku.edu)
Lysates of human chronic myelogenous leukemia cells, which had neither catalytic activity for dihydrofolate reductase nor binding of methotrexate, blocked the inhibiting effect of the antiserum on the function of the enzyme in L1210 cell lysates. (aku.edu)
Selected flavonoids were tested for their ability to inhibit the catalytic activity of DNA topoisomerase (topo) I and II. (ucy.ac.cy)
upon elevation of Ca2+ and IP4 concentrations, the PH domain binds to IP4 thereby stimulating catalytic activity, while at the same time the C2a domain takes over the role of membrane tether. (sdbonline.org)

Furthermore, in tumour necrosis factor alpha (TNFa)-stimulated Ewing's sarcoma (ES) cells, NF-jB is reported to prevent activation of an apoptotic cascade through inhibition of c-Jun N-terminal kinase (JNK) [23] and upregulation of anti-oxidant enzymes [24]. (lsd1receptor.com)

Recent studies have demonstrated a correlation between mammalian cell cytotoxicity of the quinolones and the induction of micronuclei. (wikipedia.org)
Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)

This is facilitated by glycolytic enzymes such as the M2 isoform of pyruvate kinase (PKM2) which diverts metabolites into PPP [ 6 ]. (degruyter.com)
D) dCK kinase assay using L1210 nuclear and cytoplasmic fraction lysates, [3H]-dC as substrate and performed 2 hours after exposure to 3 Gy (*, P? (biomasswars.com)
Deoxycytidine kinase (dCK) is usually a rate-limiting enzyme in the dN salvage pathway, capable of phosphorylating deoxycytidine (dC), deoxyadenosine (dA) and deoxyguanosine (dG) [3], [4]. (biomasswars.com)

Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNÎ³ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)
Typical orthotopic disease models include breast cancer, lung cancer, colon cancer, kidney cancer and diffuse large B-cell lymphoma (DLBCL), utilising mice, rats and hamsters as test subjects. (medicilon.com)

a complete loss of MMDX activity was observed in liver metastases-bearing animals receiving 40 microg/kg MMDX i.v. plus TAO. (unboundmedicine.com)

In mice with L1210 leukemia and in mice bearing EMT6 tumors, the antineoplastic action of DAC also increased with the dose. (biomedcentral.com)

Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
M5076 tumors in C57BL/6 mice and to prolong survival of DBA/2 mice with disseminated L1210 leukemia. (unboundmedicine.com)
NF-jB knockdown did not induce death under normal growth conditions, but did reduce TNFa-dependent cell survival. (lsd1receptor.com)
Around the same time, research began to show that the regulation of iron is a central function of the immune system, and that this seems to have evolved because iron is a basic requirement for the survival and growth of cells of all types, including bacteria, parasites, and cancer. (raypeatforum.com)

consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
Recent research has shown that GPNA can inhibit multiple other amino acid transporters and that GPNA's inhibition of cell viability is likely due to off-target effects from the activity of the γ-Glutamyltransferase enzyme rather than the disruption of glutamine metabolism [ 12 ] . (encyclopedia.pub)
In comparison with GPNA, V-9032 has approximately 100-fold increased potency in blocking cellular glutamine uptake, and inhibition of SLC1A5 with V-9032 was demonstrated to cause cell death, disrupt redox equilibrium, and result in disrupted development and progression of cancer [ 13 ] . (encyclopedia.pub)
In addition, dCK-deficient leukemia cell lines and ABT-888 (Veliparib) murine embryonic fibroblasts exhibited increased sensitivity to IR, indicating that pharmacologic inhibition of dCK may be an effective radiosensitization strategy. (biomasswars.com)
The reactivation of the TSGs ( p57KIP2 in HL-60 leukemic cells and p16CDKN2A in Calu-6 lung carcinoma cells) and the inhibition of global DNA methylation in HL-60 leukemic cells increased with DAC concentration. (biomedcentral.com)
The in vitro antineoplastic action of DAC correlated well with its inhibition of DNA methylation and reactivation of TSG in human neoplastic cells. (biomedcentral.com)

2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. (jenabioscience.com)
the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. (liverpool.ac.uk)
an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)

This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
Laetrile has been used for cancer treatment both as a single agent and in combination with a metabolic therapy program that consists of a specialized diet, high-dose vitamin supplements, and pancreatic enzymes . (cancer.gov)
By lowering the activation energy needed by cells to start metabolic reactions, enzymes allow biological systems to undertake necessary processes at the temperatures that exist inside the cell. (findbinaryoption.com)

It functions by inhibiting DNA gyrase, a type II topoisomerase, and topoisomerase IV, enzymes necessary to separate bacterial DNA, thereby inhibiting cell division. (wikipedia.org)
The effects of WR-1065 (2-((aminopropyl)amino)ethanethiol) on cell cycle progression, topoisomerase (topo) IIα activity, and topo IIα phosphorylation in Chinese hamster ovary (CHO) cells have been investigated. (ucy.ac.cy)

In vivo antitumor activity experiments revealed that TAO completely suppressed the ability of 90 microg/kg MMDX i.v., a dose close to the LD10, to delay growth of s.c. (unboundmedicine.com)

Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
The exact mechanism of fen-retinide-induced cell death is not fully understood, although it is reported to induce apoptosis indepen-dently of retinoic acid receptor expression [6-9], lacking the carboxyl group required for binding to these receptors [10]. (lsd1receptor.com)
These coagulation disorders are usually reversed signl rewarming.enzymes, receptors, pathological deposition), molecular imaging probes can be divided into a few large groups: 1. (findbinaryoption.com)

We have shown that intensification of the DAC dose markedly increased its antineoplastic activity in mouse models of cancer. (biomedcentral.com)

In order to restrict the proliferation of glutamine-addicted cancer cells, researchers have created various drugs that target different stages in glutamine metabolism. (encyclopedia.pub)
The assessment of cell proliferation and migration was conducted using EdU proliferation, wound healing, and transwell assays. (bvsalud.org)
CXCR7 agonist stimulated the migration of LTS derived fibroblasts significantly in vitro, with no significant influence on the cell proliferation and apoptosis. (bvsalud.org)

A hydrolase enzyme that converts L-asparagine and water to L-aspartate and NH3. (lookformedical.com)
DNMT1 operates on hemi-methylated DNA during replication, where the template strand is already methylated, but the newly synthesized strand is usually lacking methylation.27 As such, Actinomycin D biological activity DNMT1 converts the methylation of dC into an inheritable adjustment that may be transferred during duplication.28,29 Open up in another window Body 2. (thebiotechdictionary.com)

However, pregnenolone-16alpha-carbonitrile pretreatment failed to enhance MMDX activity in mice bearing either s.c. (unboundmedicine.com)
This is associated with increased tumourigenicity of ES cells in nude mice [25]. (lsd1receptor.com)

The present study examined the role of CYP3A-mediated metabolism in the in vivo antitumor activity and host toxicity of MMDX in the mouse model and investigated the potential for increasing the therapeutic effectiveness of the drug by inducing its hepatic CYP-catalyzed activation. (unboundmedicine.com)
Taken together, these findings demonstrate that an active metabolite(s) of MMDX synthesized via CYP3A contributes significantly to its in vivo antitumor activity and host toxicity. (unboundmedicine.com)

Laetrile has shown little anticancer activity in animal studies and no anticancer activity in human clinical trials. (cancer.gov)

in vitro and in vivo antiviral activity of 2'-fluorinated arabinosides of 5-(2-haloalkyl)uracil. (liverpool.ac.uk)
5-(2-fluoroethyl)-2'-deoxyuridine (fedu), its 2'-fluoroarabinofuranosyl analog (fefau) and the 2'-fluoroarabinofuranosyl analog (cefau) of the potent anti-herpesvirus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro and in vivo. (liverpool.ac.uk)

It interferes with nucleic acid synthesis by inhibiting purine metabolism and is used, usually in combination with other drugs, in the treatment of or in remission maintenance programs for leukemia. (lookformedical.com)

The NF-jB family of transcription factors are involved in the regulation of several physiological processes such as inflammation, cell cycle regulation and apoptosis. (lsd1receptor.com)

Phosphorylation of serine 74 (Ser74) was shown to be critical in regulating enzyme activity [18]C[20]. (biomasswars.com)
More recently, fenretinide-induced apoptosis has been reported to be dependent on phosphorylation of nuclear factor-kappa B (NF-jB) in SH-SY5Y neuroblastoma cells [13]. (lsd1receptor.com)
specific phosphorylation of 5-ethyl-2'-deoxyuridine by herpes simplex virus-infected cells and incorporation into viral dna. (liverpool.ac.uk)

on the contrary, phenols not substrates of tyrosinase are stable in medium and their toxicity is not reduced by scavenger enzymes. (comparewords.com)
Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)

Consistent with this hypothesis, NF-jB is constitutively acti-vated in a wide variety of tumours permitting cells to evade apoptosis [16-19,21,22]. (lsd1receptor.com)

Resolution of these problems has been approached in Drosophila by carrying out an in vivo functional analysis of Drosophila Gap1 in the context of cone cell determination in the developing Drosophila eye. (sdbonline.org)

2005) Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by zebularine on L1210 leukemia. (jenabioscience.com)
In order to clarify the dose-schedule of DAC as a guide to clinical trials, we have evaluated the antineoplastic activity of DAC both in vitro and in animal models. (biomedcentral.com)

Treatment of drug-resistant Caco-2 cells with the most active C. jambhiri and C. pyriformis compounds increased their sensitivity to doxorubicin and completely reversed doxorubicin resistance, which agrees with a decreased P- gp activity. (gotomydoctor.com)
This review report displays the structures of new compounds isolated from algae-derived and sponge-derived fungi and their activities, covering the literature from 2017 to 2021. (japsonline.com)
Several compounds showed stronger activity than the control. (japsonline.com)

Although quinolones are highly toxic to mammalian cells in culture, its mechanism of cytotoxic action is not known. (wikipedia.org)
One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)

Perwaiz Iqbal, M & Rothenberg, SP 1981, ' A nonfunctional protein in human leukemia cells reacts with antiserum to dihydrofolate reductase from L1210 leukemia cells ', Life Sciences , vol. 29, no. 7, pp. 689-696. (aku.edu)
4F10 was against the Epstein-Barr virus (EBV) Zta protein and 311 H specifically recognized EBV DNase enzyme. (karger.com)
Using mAb 4F10 as a probe, the Zta protein could be detected as a 36-kD molecule in L5 cells and as a 38-kD molecule in B95-8 cells, reflecting the fact reported by other laboratories, using rabbit polyclonal antisera, that the Zta protein was variously modified in different host cells. (karger.com)

Because cancer cells' reliance on glutamine metabolism is generally greater than in normal tissues, and glutamine deprivation in cancer cell cultures frequently results in cell death, scientists have explored depleting glutamine as a cancer therapy strategy. (encyclopedia.pub)

Fenretinide (N-(4-hydroxyphenyl)retinamide) is a synthetic vitamin A analogue with chemopreven-tive and therapeutic activity against a wide range of cancers [1]. (lsd1receptor.com)

This mechanism can also affect mammalian cell replication. (wikipedia.org)

A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. (lookformedical.com)
To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)

In double immunodiffusion, these human leukemia cell lysates formed a single precipitin line against the antiserum. (aku.edu)
20) The entropy of activation of kcat for the human enzyme was further decomposed into partially compensating electrostatic(es) (delta S*es = +15.1 cal mol-1 K-1) and nonelectrostatic(nes) (delta S*nes = -19.1 cal mol-1 K-1) terms. (comparewords.com)
infection of human epidermoid carcinoma no. 2 cells with herpes simplex virus type 1 (hsv-1) leads to a reorganization of antigens associated with both the small and heterogeneous nuclear ribonucleoprotein complexes (snrnp and hnrnp). (liverpool.ac.uk)
it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. (liverpool.ac.uk)

It kills bacteria by interfering with the enzymes that cause DNA to unwind and duplicate. (wikipedia.org)

With the goal of further improving cytokine-based immunotherapy, we examined the effects of a soybean peptide called lunasin that synergistically works with cytokines on natural killer (NK) cells. (weeksmd.com)
The key role in the pathogenesis of sarcoidosis is played by proinflammatory cytokines and chemokines, molecules crucially involved in the activation of immune and inflammatory cells and their trafficking to the site of disease [4]. (thebiotechdictionary.com)

BAY 11-7082 decreased cell viability, which was accompanied by caspase-3 cleavage. (lsd1receptor.com)

The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
BAY 11-7082-induced cell death through an NF-jB-independent mechanism and enhanced cell death when combined with fenretinide. (lsd1receptor.com)

Functional research are needed to Ezetimibe biological activity confirm biological relevance of the obtained relationships. (thebiotechdictionary.com)

Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
2009) Synthesis of a Labeled RGD-Lipid, Its Incorporation into Liposomal Nanoparticles, and Their Trafficking in Cultured Endothelial Cells. (ubc.ca)

SAM as nature's methyl iodide is usually hence reactive enough to methylate even poor nucleophilic centers such Actinomycin D biological activity as the exocyclic amino groups of A, which feature, as an sp2-hybridized N-atom only a very poor nucleophilic lone pair at the system. (thebiotechdictionary.com)

The first-generation fluoroquinolone agents, such as flumequine, had poor distribution into the body tissues and limited activity. (wikipedia.org)

Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)

Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)

Chemistry of RNA and DNA base methylation The addition of the methyl-group to DNA and RNA bases (Fig.?2) is catalyzed by DNA- and RNA-methyltransferases that use DNMTs, which methylate canonical dC bases.26 In contrast, DNMT1 maintains the methylation status Actinomycin D biological activity during cell division. (thebiotechdictionary.com)

The pioneer researcher in the role of iron in immunity believed that an excess of dietary iron contributed to the development of leukemia and lymphatic cancers. (raypeatforum.com)

In most cell types, NF-jB is sequestered in the cytoplasm by association with IjBa [20]. (lsd1receptor.com)

3). (C) Western blot of nuclear (N) and cytoplasmic (C) fractions of L1210 cell line before and 2 hours after 3 Gy exposure. (biomasswars.com)
2005). Investivgation of factors responsible for cell line cytoplasmic expression differences. (ubc.ca)

In another review in 2022, Gao and Zhang (2022) reported a total of 196 new metabolites isolated from algae-associated fungi covering from 2016 to 2021, including the chemical diversity and biological activities. (japsonline.com)
Bilirubin is a chemical that is produced when old red blood cells break down. (webmd.com)

Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
Basal levels of phosphorylated NF-jB were low in all ESFT cells. (lsd1receptor.com)

Anatomy27

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