• Protein
  • In a facile and potentially general method for protein modification at the C‐terminus, aldehyde‐modified proteins, obtained from enzymatic protein prenylation, react rapidly with hydrazide and aminooxy surfaces and fluorophores at neutral pH and in micromolar concentration ranges of reagents. (currentprotocols.com)
  • In this work, we present the structure of the bacterial LPLAT protein LpxM, and we describe a high-throughput, label-free mass spectrometric assay to characterize acyltransferase enzymatic activity. (pnas.org)
  • This problem is exacerbated by enzymatic digestion of a protein sample into a large number of peptide products. (thermofisher.com)
  • functional groups
  • In contrast to these RNA labeling methods, introducing an artificial, extra base pair (unnatural base pair), as a third base pair, to in vitro transcription systems allows the site-specific incorporation of an unnatural base linked with functional groups into desired positions of RNA during transcription mediated by the unnatural base pair. (hindawi.com)
  • analysis
  • Considerations associated with sample derivatisation strategies, ionisation, relative quantitation through isotopic as well as isobaric labelling, metabolic/enzymatic incorporation and targeted analysis are all thoroughly discussed. (rsc.org)