Colicins are protein antibiotics produced by strains of Escherichia coli to kill non-producing strains of the same organims presumably as a method of competitive inhibition. (nottingham.ac.uk)
Here, we describe detailed protocols for producing and characterizing colicins-antimicrobial proteins that are produced by Escherichia coli hosts and inactivate nonhost E. coli strains. (bvsalud.org)
Proteins1
Active colicins can be produced with lysates containing molecular chaperones and coproduction of immunity proteins in cell-free protein synthesis reactions. (bvsalud.org)
Coli3
anp S. E. LURIA Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 Received for publication 7 October 1968 The effect of colicins E1 and K on active transport of 8-p-galactosides and of a-methyl-p-glucoside (aMG) by Escherichia coli was studied. (nih.gov)
These findings, interpreted in terms of current hypotheses of permease function (7, 9, 22), led to further studies on the effects of the colicins on aerobic catabolism of E. coli, the results of which are presented in a separate article (6). (nih.gov)
Here, we describe detailed protocols for producing and characterizing colicins-antimicrobial proteins that are produced by Escherichia coli hosts and inactivate nonhost E. coli strains. (bvsalud.org)
Protein3
Of note, transcription of immunity protein for both colicins was not upregulated in any of the conditions studied except for Colicin Ia in human serum. (vegfrinhibitors.com)
Some of the iron transporters that we study also facilitate the uptake of large protein toxins called colicins. (nih.gov)
Active colicins can be produced with lysates containing molecular chaperones and coproduction of immunity proteins in cell-free protein synthesis reactions. (bvsalud.org)
Mechanism1
The findings were interpreted by as- suming that colicins E1 and K inhibit the energy-dependent steps in the accumula- tion of TMG but do not affect facilitated diffusion of galactosides mediated by the specific transport mechanism. (nih.gov)
Transport2
Printed in U.S.A. Effects of Colicins E1 and K on Transport Systems KAY L. FIELDS! (nih.gov)
These effects of the colicins on transport resemble qualitatively those of sodium azide. (nih.gov)
Found1
Luria (14) found that colicins E1(ML) and K(K235) inhibited the accumulation of substrates by several specific "permease" systems (2) and interpreted this inhibition in terms of the effects on energy metabolism observed by Levinthal and Levinthal. (nih.gov)
Conditions1
The accumulation of aMG was highly resistant to inhibition by these colicins under conditions which caused com- plete suppression of TMG accumulation. (nih.gov)