Invasive fungal diseases (IFDs) still represent a relevant cause of mortality in patients affected by hematological malignancies, especially acute myeloid leukaemia (AML) and myelodysplastic syndrome (MDS) undergoing remission induction chemotherapy, and in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. (bvsalud.org)
Cell Transplantation12
The following chapter describes the underlying biology and clinical techniques involved in the process of stem and progenitor cell identification, enumeration, mobilization, and collection prior to autologous stem cell transplantation (ASCT). (wustl.edu)
In Thomas' Hematopoietic Cell Transplantation: Fifth Edition (Vol. 1-2, pp. 452-462). (wustl.edu)
Stem cell transplantation is performed after high-dose chemotherapy (HDC) to restore a cancer patient's blood and immune cell production capacity. (bdbiosciences.com)
This phase I trial assessed the safety, efficacy, and immunologic responses to minor histocompatibility antigens following nonmyeloablative allogeneic hematopoietic cell transplantation as treatment for metastatic renal cell carcinoma. (aacrjournals.org)
Eight patients received conditioning with fludarabine and low-dose total body irradiation followed by hematopoietic cell transplantation from an HLA-matched sibling donor. (aacrjournals.org)
Treatment of metastatic renal cell carcinoma with allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning with fludarabine/total body irradiation is feasible and may induce tumor regression or stabilization in some patients. (aacrjournals.org)
this is used in both autologous and allogenic HSCT. (bdbiosciences.com)
Progenitor4
CD34, a transmembrane phosphoglycoprotein, is present on immature hematopoietic precursor cells and all hematopoietic colony-forming cells in bone marrow and blood, including unipotent and pluripotent progenitor cells. (bdbiosciences.com)
For many hematopoietic malignancies, collection and infusion of CD34+ hematopoietic stem/progenitor cells following chemotherapy is critical. (bdbiosciences.com)
Flow cytometric enumeration of CD34+ HSCs and progenitor cells is an established method for the evaluation of bone marrow and stem cell grafts. (bdbiosciences.com)
Flow cytometric applications for CD34+ cell identification and enumeration provide a rapid, quantitative and reproducible method to evaluate the progenitor cell population. (bdbiosciences.com)
Autoimmune1
SCID patients presenting with circulating T cells show different patterns of T-cell activity and regulatory T cells enumeration that dictates the immunodeficient and autoimmune manifestations. (hindawi.com)
Maternal1
In some cases, T cells may present, as a result of residual autologous cells or transplacentally acquired maternal lymphocytes [ 1 ]. (hindawi.com)
Letermovir prophylaxis revolutionized the approach to Cytomegalovirus infection in adult hematopoietic stem cell transplant (HCT), while data in pediatric setting are still lacking. (bvsalud.org)
Patients2
All patients achieved initial mixed hematopoietic chimerism with two patients rejecting their graft and recovering host hematopoiesis. (aacrjournals.org)
MHC-II deficient patients had slightly reduced T-cell function, normal TRECs, TCR repertoires, and normal Tregs enumeration. (hindawi.com)
Blood3
Shea, TC & DiPersio, JF 2016, Mobilization of Peripheral Blood Hematopoietic Cells for Autologous HCT . (wustl.edu)
This pharmacologically induced egress of HSCs into peripheral blood, called mobilization, is utilized as the preferred strategy for generating HSCs for transplantation. (bdbiosciences.com)
Professor Ma holds leadership or scientific membership positions in a number of national and international scientific communities including the World Network for Blood & Marrow Transplantation (a WHO affiliated NGO), the AsiaPacific BM Transplant and the Royal College of Pathology of Australasian Quality Assurance Programme. (edu.au)
4 CD34 expression is historically related to hematopoietic cells and it is considered as the marker of HSCs. (bdbiosciences.com)
BD Biosciences offers flow cytometers and assays for the enumeration of CD34+ cells. (bdbiosciences.com)
The BD ® Stem Cell Enumeration (SCE) Assay enumerates total viable CD34+ and CD45+ cells as a percentage of total viable leukocytes. (bdbiosciences.com)
Here we compared T-cell functions including the number of circulating CD3 + T cells, in vitro responses to mitogens, T-cell receptor (TCR) repertoire, TCR excision circles (TREC) levels, and regulatory T cells (Tregs) enumeration in several immunodeficinecy subtypes, clinically presenting with nonreactive residual cells (MHC-II deficiency) or reactive cells. (hindawi.com)
Residual autologous T cells are usually emerging from partial thymic maturation impairment such as in the case of Major histocompatibility complex class II (MHC-II) deficiency. (hindawi.com)
Techniques2
1 Improvements in transplantation techniques, including the wider use of cell selection, have contributed to a significant reduction in the morbidity and mortality associated with conventional transplantation. (bdbiosciences.com)
these include the improvement of diagnoses, the expanding treatment landscape of AML with molecular targeted drugs (and related drug-drug interactions with azoles), evolving transplantation techniques (and their related impacts on IFDs' risk stratification), and new antifungals and their features (rezafungin and olorofim). (bvsalud.org)
3. Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation. (nih.gov)
Autologous peripheral blood stem cellstransplantation (APBSCT) is a therapeutic option which can be used in various hematological, neoplastic disorders including lymphoproliferative disease (LPD). (omjournal.org)
All patients had a confirmed diagnosis of multiple myeloma and were enrolled in the same aggressive tandem autologous peripheral blood stem cell transplant protocol with or without the addition of thalidomide. (medscape.com)
Stem cells5
10. Extensive early apoptosis in frozen-thawed CD34-positive stem cells decreases threshold doses for haematological recovery after autologous peripheral blood progenitor cell transplantation. (nih.gov)
Hematopoietic stem cells (HSCs) are the functional units in bone marrow transplantation (BMT) and gene therapy approaches via their ability for multilineage differentiation and long-term self-renewal 1 . (elifesciences.org)
See "Overview of hematopoietic stem cells" . (medilib.ir)
Engraftment1
Patients with LPD who presented at an early stage and with no history of radiotherapy had faster neutrophil engraftment after APBSCT, while a younger age at transplantation with a higher dose of CD34+ cells may predict faster platelet engraftment. (omjournal.org)
Platelet1
Presumably, WASp mutations interfere with the proper signaling and growth of cells of the hematopoietic lineage, resulting in the platelet and immune defects observed clinically, although the exact mechanisms and defective pathways remain largely unknown. (medscape.com)
8. Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells in leukapheresis product and bone marrow for clinical transplantation: a comparison of three methods. (nih.gov)
The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. (nih.gov)
Bone marrow hematopoiesis commences by about five months of gestation and under normal circumstances is the primary source of hematopoietic cells after birth. (medilib.ir)
Dose1
15. Determining post-thaw CD34+ cell dose of cryopreserved haematopoietic progenitor cells demonstrates high recovery and confirms their integrity. (nih.gov)
Resistance1
17. Peripheral blood and BM CD34+ CD38- cells show better resistance to cryopreservation than CD34+ CD38+ cells in autologous stem cell transplantation. (nih.gov)
Potential1
4. Impact of different dimethyl sulphoxide concentrations on cell recovery, viability and clonogenic potential of cryopreserved peripheral blood hematopoietic stem and progenitor cells. (nih.gov)
Time4
7. Effects of incubation temperature and time after thawing on viability assessment of peripheral hematopoietic progenitor cells cryopreserved for transplantation. (nih.gov)
13. Automated dry thawing of cryopreserved haematopoietic cells is not adversely influenced by cryostorage time, patient age or gender. (nih.gov)
At the same time, alternative assays such as limit dilution assays or single-cell transplantation assays are cumbersome, expensive and associated with ethical concerns. (elifesciences.org)
We tagged rhesus macaque autologous HSPCs with genetic barcodes, allowing clonal tracking over time and space after transplantation. (nih.gov)
Units1
Within Lincoln and Guba's paradigm, content analysis of the data focuses on units of meaning within their context rather than enumeration of constructs. (medscape.com)